RLG00000027004

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003557
Physical Location & Seq
Forward (+)
7631008 .. 7634575
3568 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027004

Sequence Viewer

Length: 1248 bp
ATGTTCTCATCCTCATGGTTGAAATTACCGAGCAAGTTAAACAAACCCGCACCAGCATCGCGTTCGTACAGTAGCATTTCTTGCTCATCCTTCAAAGACATCCAAACCCTCTGCAAATCTCAACCAGAAACCGAGGTACTCAGCCCGAGGAGTGCCTCCATTTTGCAGCGTATCAAGATGTCCACCACGGTCCTCCTGCGCCTCTGGGCTCGCGGCGCAGGGGGCCCAAGAGGTTGCAAAGTGCCCAACGGAGACCAACGTGTAGTGATCTACTACACGAGTCTCCGCGTGGTGCGGAGGACCTTTGAGGATTGCAGGGTAGTCAGATCGATCCTCGGAGGGTTTCGGGTCCCCATTGACGAACGAGATCTGTCAATGGACTCCAAGTTTGTGCAGGAGCTGGAGGAGGTCACTGGGAGCAAGGACTTGAACTTGCCCATGGTTTTCGTCAGCGGAGACTACATTGGTGGCGCCGAGGAGGTGAGGCAGCTCCATGATAGCGGAGAGCTGAAGACAATGCTCCAAGGGCTCCCGGTGGTGGAGTCCAAAACTTGTGATGTGTGCGGAGGGCTGAGATTTATTCTCTGCCAGACTTGCAACGGAAGCCACAAACTCTACAGACAGATAGATGGGTTTTTCCCTTACATCCAGAGCTTGTTCCAACCCGATCCTGAAGCCCTCACCCCGAGGAGAGCCTCTATCATACATCGGGCCAAGACATCCACCTCAGTCTTTCGTTTGTGGGCACACCACATTGCGCGCCCAAGTAGGATACACGACGATGATCGCTGTGTGGTAATCTACTATACGAGCCTACGTGTAGTGCGCAGAACTTACGAGGATTGTAAGGTTGTCCGATCAATTTTCAGAGGATTTCGTGTTCCCATTGACGAACGAGATTTATCAATGGACACAAAAATTTTCGAGGAGTTGCAAGAGCTTATGGGAAGTAGGACCTTCACTTTGCCTATGGTCTTCATCAGTGGGATATGCATTGGTGGGGTTGAAGAGATCCGACAACTCCATGAAAGTGGTGATCTGAAGGTAATGATGCAATGGCTACCGGTGGTAGACTCTAGACCGTGTGACTTATGTGGAGGAATCAAGTTTGTGCTATGCCAAACATGTGATGGAAGTCATAGAGTCCGTTGTGACGGTGAATTTAGTCCTTGCACAACTTGTAATGTGAATGGTCTAGTTATGTGTCCGTCATGTTCTACCATGCGGTGCCGGATCTCACCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

416

Amino Acids

46.79

Weight (kDa)

8.75

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 89 - 155 2e-08 Glutaredoxin
GRXCR1-2_C PF23733 185 - 208 2.2e-09 GRXCR1/2, C-terminal domain
Glutaredoxin PF00462 266 - 329 6e-10 Glutaredoxin
GRXCR1-2_C PF23733 362 - 406 4.5e-14 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 827
AccB1I GGYRCC 2 cut(s) 470, 1227
AccI GTMKAC 1 cut(s) 1071
AccII CGCG 4 cut(s) 61, 213, 288, 760
AciI CCGC 8 cut(s) 48, 213, 286, 295, 453, 501, 564, 1225
AclWI GGATC 4 cut(s) 325, 662, 1006, 1241
AcsI RAATTY 2 cut(s) 918, 1160
AcuI CTGAAG 3 cut(s) 530, 693, 1061
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 2 cut(s) 68, 138
AfiI CCNNNNNNNGG 1 cut(s) 538
AflIII ACRYGT 3 cut(s) 259, 817, 1124
AgeI ACCGGT 1 cut(s) 1063
AgsI TTSAA 4 cut(s) 22, 94, 430, 1007
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
AluBI AGCT 5 cut(s) 400, 490, 508, 654, 940
AluI AGCT 5 cut(s) 400, 490, 508, 654, 940
Alw26I GTCTC 3 cut(s) 246, 287, 450
AlwI GGATC 4 cut(s) 325, 662, 1006, 1241
AlwNI CAGNNNCTG 1 cut(s) 400
Ama87I CYCGRG 2 cut(s) 145, 685
AoxI GGCC 2 cut(s) 223, 711
ApaI GGGCCC 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 166, 487
ApoI RAATTY 2 cut(s) 918, 1160
ArsI GACNNNNNNTTYG 4 cut(s) 371, 403, 946, 978
AsiGI ACCGGT 1 cut(s) 1063
AspLEI GCGC 6 cut(s) 201, 218, 473, 760, 762, 828
AspS9I GGNCC 7 cut(s) 190, 223, 224, 300, 349, 711, 954
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 5 cut(s) 493, 673, 1046, 1169, 1230
AvaI CYCGRG 2 cut(s) 145, 685
AvaII GGWCC 4 cut(s) 190, 300, 349, 954
BaeGI GKGCMC 3 cut(s) 227, 246, 748
BanI GGYRCC 2 cut(s) 470, 1227
BanII GRGCYC 3 cut(s) 211, 227, 531
BauI CACGAG 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 518, 967
BbvI GCAGC 2 cut(s) 178, 499
BccI CCATC 2 cut(s) 623, 1124
BcgI CGANNNNNNTGC 4 cut(s) 39, 45, 73, 79
BciVI GTATCC 1 cut(s) 765
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 246, 287, 450
BfaI CTAG 2 cut(s) 1077, 1196
BfmI CTRYAG 1 cut(s) 616
BfoI RGCGCY 1 cut(s) 474
BfuI GTATCC 1 cut(s) 765
BglII AGATCT 1 cut(s) 367
BisI GCNGC 3 cut(s) 167, 214, 488
BlsI GCNGC 3 cut(s) 168, 215, 489
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 4 cut(s) 190, 300, 349, 954
BmeT110I CYCGRG 2 cut(s) 145, 685
BmgT120I GGNCC 7 cut(s) 190, 223, 224, 300, 349, 711, 954
BmiI GGNNCC 7 cut(s) 224, 225, 350, 351, 472, 530, 1229
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 2 cut(s) 65, 1041
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 518, 967
BpmI CTGGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 533
Bsa29I ATCGAT 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 818
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 8 cut(s) 132, 146, 186, 334, 438, 474, 523, 686
BsaWI WCCGGW 1 cut(s) 1063
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 1063
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 2 cut(s) 753, 1061
BseCI ATCGAT 1 cut(s) 329
BseDI CCNNGG 8 cut(s) 132, 146, 186, 334, 438, 474, 523, 686
BseGI GGATG 5 cut(s) 8, 86, 99, 645, 719
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 2 cut(s) 753, 1061
BseMII CTCAG 3 cut(s) 154, 563, 741
BseNI ACTGG 1 cut(s) 418
BsePI GCGCGC 1 cut(s) 758
BseRI GAGGAG 5 cut(s) 163, 419, 491, 703, 941
BseSI GKGCMC 3 cut(s) 227, 246, 748
BseXI GCAGC 2 cut(s) 178, 499
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 4 cut(s) 61, 213, 288, 760
BshFI GGCC 2 cut(s) 225, 713
BshNI GGYRCC 2 cut(s) 470, 1227
BshTI ACCGGT 1 cut(s) 1063
BshVI ATCGAT 1 cut(s) 329
BsiHKCI CYCGRG 2 cut(s) 145, 685
BsiSI CCGG 3 cut(s) 533, 1064, 1231
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 3 cut(s) 246, 287, 450
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 2 cut(s) 225, 713
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 2 cut(s) 145, 685
Bsp120I GGGCCC 1 cut(s) 223
Bsp1286I GDGCHC 5 cut(s) 211, 227, 246, 531, 748
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 8 cut(s) 48, 213, 286, 295, 453, 501, 564, 1225
BspANI GGCC 2 cut(s) 225, 713
BspCNI CTCAG 3 cut(s) 153, 564, 740
BspDI ATCGAT 1 cut(s) 329
BspFNI CGCG 4 cut(s) 61, 213, 288, 760
BspLI GGNNCC 7 cut(s) 224, 225, 350, 351, 472, 530, 1229
BspPI GGATC 4 cut(s) 325, 662, 1006, 1241
BspT107I GGYRCC 2 cut(s) 470, 1227
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 753, 1061
BsrFI RCCGGY 1 cut(s) 1063
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 8 cut(s) 132, 146, 186, 334, 438, 474, 523, 686
BssHII GCGCGC 1 cut(s) 758
BssNI GRCGYC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 2 cut(s) 438, 523
Bst2BI CACGAG 1 cut(s) 277
Bst4CI ACNGT 4 cut(s) 71, 190, 1083, 1157
Bst6I CTCTTC 1 cut(s) 1002
BstACI GRCGYC 1 cut(s) 471
BstAPI GCANNNNNTGC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 211, 760
BstDEI CTNAG 3 cut(s) 140, 572, 727
BstDSI CCRYGG 2 cut(s) 186, 438
BstF5I GGATG 5 cut(s) 8, 86, 99, 645, 719
BstFNI CGCG 4 cut(s) 61, 213, 288, 760
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 6 cut(s) 201, 218, 473, 760, 762, 828
BstMAI GTCTC 3 cut(s) 246, 287, 450
BstMWI GCNNNNNNNGC 6 cut(s) 81, 215, 222, 526, 594, 603
BstNSI RCATGY 1 cut(s) 1128
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 616
BstSLI GKGCMC 3 cut(s) 227, 246, 748
BstUI CGCG 4 cut(s) 61, 213, 288, 760
BstV1I GCAGC 2 cut(s) 178, 499
BstV2I GAAGAC 2 cut(s) 518, 967
BstX2I RGATCY 3 cut(s) 367, 1011, 1233
BstXI CCANNNNNNTGG 1 cut(s) 1031
BstYI RGATCY 3 cut(s) 367, 1011, 1233
Bsu15I ATCGAT 1 cut(s) 329
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 2 cut(s) 225, 713
BsuTUI ATCGAT 1 cut(s) 329
BtgI CCRYGG 2 cut(s) 186, 438
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 5 cut(s) 8, 86, 99, 645, 719
BtsIMutI CAGTG 2 cut(s) 411, 988
Cac8I GCNNGC 2 cut(s) 211, 760
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 6 cut(s) 201, 218, 473, 760, 762, 828
Cfr10I RCCGGY 1 cut(s) 1063
Cfr13I GGNCC 7 cut(s) 190, 223, 224, 300, 349, 711, 954
ClaI ATCGAT 1 cut(s) 329
Csp6I GTAC 2 cut(s) 67, 137
CspAI ACCGGT 1 cut(s) 1063
CviAII CATG 7 cut(s) 15, 439, 494, 1025, 1125, 1212, 1222
CviQI GTAC 2 cut(s) 67, 137
DdeI CTNAG 3 cut(s) 140, 572, 727
DinI GGCGCC 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
Eco130I CCWWGG 2 cut(s) 438, 523
Eco24I GRGCYC 3 cut(s) 211, 227, 531
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 190, 300, 349, 954
Eco57I CTGAAG 3 cut(s) 530, 693, 1061
Eco88I CYCGRG 2 cut(s) 145, 685
EcoO109I RGGNCCY 4 cut(s) 223, 300, 349, 954
EcoT14I CCWWGG 2 cut(s) 438, 523
EcoT22I ATGCAT 1 cut(s) 995
EcoT38I GRGCYC 3 cut(s) 211, 227, 531
EgeI GGCGCC 1 cut(s) 472
EheI GGCGCC 1 cut(s) 472
ErhI CCWWGG 2 cut(s) 438, 523
FaeI CATG 7 cut(s) 18, 442, 497, 1028, 1128, 1215, 1225
FaqI GGGAC 1 cut(s) 335
FatI CATG 7 cut(s) 14, 438, 493, 1024, 1124, 1211, 1221
FauI CCCGC 1 cut(s) 55
FblI GTMKAC 1 cut(s) 1071
Fnu4HI GCNGC 3 cut(s) 167, 214, 488
FokI GGATG 4 cut(s) 73, 86, 632, 706
FriOI GRGCYC 3 cut(s) 211, 227, 531
Fsp4HI GCNGC 3 cut(s) 167, 214, 488
FspBI CTAG 2 cut(s) 1077, 1196
FspI TGCGCA 1 cut(s) 827
GlaI GCGC 6 cut(s) 200, 217, 472, 759, 761, 827
GluI GCNGC 3 cut(s) 167, 214, 488
GsuI CTGGAG 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 2 cut(s) 225, 713
HapII CCGG 3 cut(s) 533, 1064, 1231
HhaI GCGC 6 cut(s) 201, 218, 473, 760, 762, 828
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 7 cut(s) 18, 442, 497, 1028, 1128, 1215, 1225
Hin6I GCGC 6 cut(s) 199, 216, 471, 758, 760, 826
HinP1I GCGC 6 cut(s) 199, 216, 471, 758, 760, 826
HinfI GANTC 6 cut(s) 280, 380, 542, 1073, 1101, 1143
HpaII CCGG 3 cut(s) 533, 1064, 1231
HphI GGTGA 5 cut(s) 493, 673, 1046, 1169, 1230
Hpy166II GTNNAC 2 cut(s) 183, 1072
Hpy188I TCNGA 6 cut(s) 326, 338, 857, 869, 1016, 1041
Hpy188III TCNNGA 4 cut(s) 175, 649, 671, 1077
Hpy8I GTNNAC 2 cut(s) 183, 1072
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 3 cut(s) 100, 967, 1036
HpyCH4III ACNGT 4 cut(s) 71, 190, 1083, 1157
HpyCH4IV ACGT 2 cut(s) 259, 817
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 215, 222, 526, 594, 603
HpyF3I CTNAG 3 cut(s) 140, 572, 727
HpySE526I ACGT 2 cut(s) 259, 817
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 7 cut(s) 18, 442, 497, 1028, 1128, 1215, 1225
HspAI GCGC 6 cut(s) 199, 216, 471, 758, 760, 826
KasI GGCGCC 1 cut(s) 470
KflI GGGWCCC 1 cut(s) 349
LmnI GCTCC 5 cut(s) 397, 417, 495, 525, 534
Lsp1109I GCAGC 2 cut(s) 178, 499
LweI GCATC 2 cut(s) 65, 1041
MaeI CTAG 2 cut(s) 1077, 1196
MaeII ACGT 2 cut(s) 259, 817
MaeIII GTNAC 3 cut(s) 409, 1085, 1151
MboII GAAGA 3 cut(s) 523, 967, 1019
MflI RGATCY 3 cut(s) 367, 1011, 1233
MhlI GDGCHC 5 cut(s) 211, 227, 246, 531, 748
MluCI AATT 4 cut(s) 23, 861, 918, 1160
Mly113I GGCGCC 1 cut(s) 471
MlyI GAGTC 5 cut(s) 289, 374, 551, 1067, 1152
MmeI TCCRAC 2 cut(s) 685, 1039
Mph1103I ATGCAT 1 cut(s) 995
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 3 cut(s) 13, 780, 1029
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 3 cut(s) 533, 1064, 1231
MspR9I CCNGG 1 cut(s) 533
MvnI CGCG 4 cut(s) 61, 213, 288, 760
MwoI GCNNNNNNNGC 6 cut(s) 81, 215, 222, 526, 594, 603
NarI GGCGCC 1 cut(s) 471
NciI CCSGG 1 cut(s) 533
NcoI CCATGG 1 cut(s) 438
NlaIII CATG 7 cut(s) 18, 442, 497, 1028, 1128, 1215, 1225
NlaIV GGNNCC 7 cut(s) 224, 225, 350, 351, 472, 530, 1229
NmeAIII GCCGAG 1 cut(s) 499
NmuCI GTSAC 3 cut(s) 409, 1085, 1151
NsbI TGCGCA 1 cut(s) 827
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 1 cut(s) 1128
PauI GCGCGC 1 cut(s) 758
PciI ACATGT 1 cut(s) 1124
PfeI GAWTC 1 cut(s) 1101
PinAI ACCGGT 1 cut(s) 1063
PkrI GCNGC 3 cut(s) 168, 215, 489
PleI GAGTC 5 cut(s) 288, 374, 550, 1067, 1151
PluTI GGCGCC 1 cut(s) 474
PpsI GAGTC 5 cut(s) 288, 374, 550, 1067, 1151
Ppu21I YACGTR 1 cut(s) 818
PpuMI RGGWCCY 3 cut(s) 300, 349, 954
PscI ACATGT 1 cut(s) 1124
Psp5II RGGWCCY 3 cut(s) 300, 349, 954
PspN4I GGNNCC 7 cut(s) 224, 225, 350, 351, 472, 530, 1229
PspOMI GGGCCC 1 cut(s) 223
PspPI GGNCC 7 cut(s) 190, 223, 224, 300, 349, 711, 954
PspPPI RGGWCCY 3 cut(s) 300, 349, 954
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 3 cut(s) 367, 1011, 1233
PteI GCGCGC 1 cut(s) 758
RsaI GTAC 2 cut(s) 68, 138
RsaNI GTAC 2 cut(s) 67, 137
RseI CAYNNNNRTG 3 cut(s) 13, 780, 1029
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 3 cut(s) 167, 214, 488
Sau96I GGNCC 7 cut(s) 190, 223, 224, 300, 349, 711, 954
SchI GAGTC 5 cut(s) 289, 374, 551, 1067, 1152
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 5 cut(s) 211, 227, 246, 531, 748
SfaNI GCATC 2 cut(s) 65, 1041
SfcI CTRYAG 1 cut(s) 616
SfoI GGCGCC 1 cut(s) 472
SinI GGWCC 4 cut(s) 190, 300, 349, 954
SmiMI CAYNNNNRTG 3 cut(s) 13, 780, 1029
Sse9I AATT 4 cut(s) 23, 861, 918, 1160
SsiI CCGC 8 cut(s) 48, 213, 286, 295, 453, 501, 564, 1225
SspDI GGCGCC 1 cut(s) 470
SspMI CTAG 2 cut(s) 1077, 1196
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 2 cut(s) 438, 523
TaaI ACNGT 4 cut(s) 71, 190, 1083, 1157
TaiI ACGT 2 cut(s) 262, 820
TaqI TCGA 2 cut(s) 329, 924
TasI AATT 4 cut(s) 23, 861, 918, 1160
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 1101
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 418, 988
TseFI GTSAC 3 cut(s) 409, 1085, 1151
TseI GCWGC 2 cut(s) 166, 487
Tsp45I GTSAC 3 cut(s) 409, 1085, 1151
TspDTI ATGAA 2 cut(s) 967, 1041
TspGWI ACGGA 4 cut(s) 264, 615, 1136, 1197
TspRI CASTG 2 cut(s) 418, 988
VpaK11BI GGWCC 4 cut(s) 190, 300, 349, 954
XapI RAATTY 2 cut(s) 918, 1160
XbaI TCTAGA 1 cut(s) 1076
XceI RCATGY 1 cut(s) 1128
XcmI CCANNNNNNNNNTGG 1 cut(s) 1127
XmiI GTMKAC 1 cut(s) 1071
XspI CTAG 2 cut(s) 1077, 1196
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.