Rroxscaffold_4G00286180

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003557
Physical Location & Seq
Forward (+)
7312498 .. 7313208
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286180.1

Sequence Viewer

Length: 711 bp
ATGGCCATGTTCTCATCCTCATGGTTGAAATTACCGAGCAAGTTAAATAAACCCGCACCAGCATCGCATTCGTACAGAAGCATTTCTTGCTCATCCTTCAAAGACATCCAAACCCTCTGCAAATCTCAACCCGAAACCGAGGTCCTCAGCCCGAGGAGTGCCTCCATTTTGCAGCGTATCAAGATGTCCACCACGGTCCTCCTGCGCCTCTGGGCTGGCGGCGCAGGGGGCCCAAGAGGCTGCAAAGTGCCCAACGGAGACCAACGTGTAGTGATCTACTACACGACTCTCCGCGTGGTGCGGAGGACCTTTGAGGATTGCAGGGTAGTCAGATCGATCCTTGGAGGGTTTCGTGTCCCCATTGACGAGCGAGATCTGTCAATGGACTCCAAGTTTGTGCAGGAGCTGGAGGAGGTCACTGGGAGCAAGGACTTGAACTTGCCCATGGTTTTCGTCAGCGGAGACTACATTGGTGGCGCCGAGGAGGTGAGGCAGCTCCATGAAAGCGGAGAGCTGAAGACAGTGCTCCAAGGGCTCCCGGTCGTGGAGTCCAAAACTTGTGATGTGTGCGGTGGGCTGAGATTTATTCTCTGCCAGACTTGCAACGGAAGCCACAAACTCTACAGACAGAAAGATGGGTTTTGCCCTTGTATGGACTGCAACATGAATGGCATGATTACGTGCCCTTCATGTTCAACTGCAGGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

236

Amino Acids

26.0

Weight (kDa)

8.68

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 91 - 157 5.7e-09 Glutaredoxin
GRXCR1-2_C PF23733 187 - 232 2.9e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccII CGCG 1 cut(s) 294
AciI CCGC 7 cut(s) 54, 219, 292, 301, 459, 507, 570
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 477
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 544, 652
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 4 cut(s) 28, 100, 436, 696
AluBI AGCT 3 cut(s) 406, 496, 514
AluI AGCT 3 cut(s) 406, 496, 514
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 2 cut(s) 252, 456
AlwI GGATC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 2 cut(s) 3, 229
ApaI GGGCCC 1 cut(s) 233
ApeKI GCWGC 3 cut(s) 172, 240, 493
ArsI GACNNNNNNTTYG 4 cut(s) 126, 158, 377, 409
Asp700I GAANNNNTTC 1 cut(s) 82
AspLEI GCGC 3 cut(s) 207, 224, 479
AspS9I GGNCC 5 cut(s) 142, 196, 229, 230, 306
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 1 cut(s) 499
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 3 cut(s) 142, 196, 306
BaeGI GKGCMC 3 cut(s) 233, 252, 686
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 2 cut(s) 233, 537
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 528
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 184, 227, 505
BccI CCATC 1 cut(s) 629
BcgI CGANNNNNNTGC 4 cut(s) 45, 51, 79, 85
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 2 cut(s) 252, 456
BfmI CTRYAG 2 cut(s) 622, 699
BfoI RGCGCY 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 237
BglII AGATCT 1 cut(s) 373
BisI GCNGC 4 cut(s) 173, 220, 241, 494
BlsI GCNGC 4 cut(s) 174, 221, 242, 495
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 3 cut(s) 142, 196, 306
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 5 cut(s) 142, 196, 229, 230, 306
BmiI GGNNCC 4 cut(s) 230, 231, 478, 536
BmrFI CCNGG 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 524
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 477
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 7 cut(s) 138, 152, 192, 340, 444, 480, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 544, 652
Bse1I ACTGG 1 cut(s) 424
BseCI ATCGAT 1 cut(s) 335
BseDI CCNNGG 7 cut(s) 138, 152, 192, 340, 444, 480, 529
BseGI GGATG 3 cut(s) 14, 92, 105
BseLI CCNNNNNNNGG 2 cut(s) 544, 652
BseMII CTCAG 2 cut(s) 160, 569
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 3 cut(s) 169, 425, 497
BseSI GKGCMC 3 cut(s) 233, 252, 686
BseXI GCAGC 3 cut(s) 184, 227, 505
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 543
BshFI GGCC 2 cut(s) 5, 231
BshNI GGYRCC 1 cut(s) 476
BshVI ATCGAT 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 2 cut(s) 544, 652
BsmAI GTCTC 2 cut(s) 252, 456
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 2 cut(s) 5, 231
Bso31I GGTCTC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 151
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 5 cut(s) 233, 252, 528, 537, 686
Bsp143I GATC 4 cut(s) 273, 332, 336, 373
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 7 cut(s) 54, 219, 292, 301, 459, 507, 570
BspANI GGCC 2 cut(s) 5, 231
BspCNI CTCAG 2 cut(s) 159, 570
BspDI ATCGAT 1 cut(s) 335
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 4 cut(s) 230, 231, 478, 536
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 424
BssECI CCNNGG 7 cut(s) 138, 152, 192, 340, 444, 480, 529
BssMI GATC 4 cut(s) 273, 332, 336, 373
BssNI GRCGYC 1 cut(s) 477
BssT1I CCWWGG 3 cut(s) 340, 444, 529
Bst4CI ACNGT 3 cut(s) 196, 523, 708
BstACI GRCGYC 1 cut(s) 477
BstAPI GCANNNNNTGC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 681
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 2 cut(s) 146, 578
BstDSI CCRYGG 2 cut(s) 192, 444
BstF5I GGATG 3 cut(s) 14, 92, 105
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 3 cut(s) 207, 224, 479
BstKTI GATC 4 cut(s) 276, 335, 339, 376
BstMAI GTCTC 2 cut(s) 252, 456
BstMBI GATC 4 cut(s) 273, 332, 336, 373
BstMCI CGRYCG 1 cut(s) 543
BstMWI GCNNNNNNNGC 7 cut(s) 87, 221, 228, 237, 532, 600, 609
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 2 cut(s) 622, 699
BstSLI GKGCMC 3 cut(s) 233, 252, 686
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 184, 227, 505
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
Bsu15I ATCGAT 1 cut(s) 335
BsuRI GGCC 2 cut(s) 5, 231
BsuTUI ATCGAT 1 cut(s) 335
BtgI CCRYGG 2 cut(s) 192, 444
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 3 cut(s) 14, 92, 105
BtsIMutI CAGTG 2 cut(s) 417, 528
Cac8I GCNNGC 1 cut(s) 217
CaiI CAGNNNCTG 1 cut(s) 406
CfoI GCGC 3 cut(s) 207, 224, 479
Cfr13I GGNCC 5 cut(s) 142, 196, 229, 230, 306
ClaI ATCGAT 1 cut(s) 335
Csp6I GTAC 1 cut(s) 73
CviAII CATG 7 cut(s) 7, 21, 445, 500, 664, 673, 690
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 2 cut(s) 146, 578
DinI GGCGCC 1 cut(s) 478
DpnI GATC 4 cut(s) 275, 334, 338, 375
DpnII GATC 4 cut(s) 273, 332, 336, 373
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 3 cut(s) 340, 444, 529
Eco24I GRGCYC 2 cut(s) 233, 537
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 3 cut(s) 142, 196, 306
Eco57I CTGAAG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 151
EcoO109I RGGNCCY 3 cut(s) 142, 229, 306
EcoT14I CCWWGG 3 cut(s) 340, 444, 529
EcoT38I GRGCYC 2 cut(s) 233, 537
EgeI GGCGCC 1 cut(s) 478
EheI GGCGCC 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 340, 444, 529
FaeI CATG 7 cut(s) 10, 24, 448, 503, 667, 676, 693
FaiI YATR 8 cut(s) 8, 22, 446, 501, 653, 665, 674, 691
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FaqI GGGAC 1 cut(s) 341
FatI CATG 7 cut(s) 6, 20, 444, 499, 663, 672, 689
FauI CCCGC 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 173, 220, 241, 494
FokI GGATG 2 cut(s) 79, 92
FriOI GRGCYC 2 cut(s) 233, 537
Fsp4HI GCNGC 4 cut(s) 173, 220, 241, 494
GlaI GCGC 3 cut(s) 206, 223, 478
GluI GCNGC 4 cut(s) 173, 220, 241, 494
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 2 cut(s) 5, 231
HapII CCGG 1 cut(s) 539
HhaI GCGC 3 cut(s) 207, 224, 479
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 7 cut(s) 10, 24, 448, 503, 667, 676, 693
Hin6I GCGC 3 cut(s) 205, 222, 477
HinP1I GCGC 3 cut(s) 205, 222, 477
HinfI GANTC 3 cut(s) 286, 386, 548
HpaII CCGG 1 cut(s) 539
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 1 cut(s) 332
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 189
HpyAV CCTTC 2 cut(s) 106, 696
HpyCH4III ACNGT 3 cut(s) 196, 523, 708
HpyCH4IV ACGT 2 cut(s) 265, 680
HpyCH4V TGCA 8 cut(s) 120, 172, 243, 321, 400, 603, 660, 701
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 221, 228, 237, 532, 600, 609
HpyF3I CTNAG 2 cut(s) 146, 578
HpySE526I ACGT 2 cut(s) 265, 680
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 7 cut(s) 10, 24, 448, 503, 667, 676, 693
HspAI GCGC 3 cut(s) 205, 222, 477
KasI GGCGCC 1 cut(s) 476
Kzo9I GATC 4 cut(s) 273, 332, 336, 373
LmnI GCTCC 5 cut(s) 403, 423, 501, 531, 540
Lsp1109I GCAGC 3 cut(s) 184, 227, 505
LweI GCATC 1 cut(s) 71
MaeII ACGT 2 cut(s) 265, 680
MaeIII GTNAC 1 cut(s) 415
MalI GATC 4 cut(s) 275, 334, 338, 375
MboI GATC 4 cut(s) 273, 332, 336, 373
MboII GAAGA 1 cut(s) 529
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 5 cut(s) 233, 252, 528, 537, 686
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 477
MlyI GAGTC 3 cut(s) 280, 380, 557
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 82
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 459
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 67
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 7 cut(s) 87, 221, 228, 237, 532, 600, 609
NarI GGCGCC 1 cut(s) 477
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 1 cut(s) 444
NdeII GATC 4 cut(s) 273, 332, 336, 373
NlaIII CATG 7 cut(s) 10, 24, 448, 503, 667, 676, 693
NlaIV GGNNCC 4 cut(s) 230, 231, 478, 536
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 1 cut(s) 415
PctI GAATGC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 82
PkrI GCNGC 4 cut(s) 174, 221, 242, 495
PleI GAGTC 3 cut(s) 280, 380, 556
PluTI GGCGCC 1 cut(s) 480
PpsI GAGTC 3 cut(s) 280, 380, 556
Ppu21I YACGTR 1 cut(s) 681
PpuMI RGGWCCY 2 cut(s) 142, 306
Psp5II RGGWCCY 2 cut(s) 142, 306
PspN4I GGNNCC 4 cut(s) 230, 231, 478, 536
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 5 cut(s) 142, 196, 229, 230, 306
PspPPI RGGWCCY 2 cut(s) 142, 306
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 4 cut(s) 173, 220, 241, 494
Sau3AI GATC 4 cut(s) 273, 332, 336, 373
Sau96I GGNCC 5 cut(s) 142, 196, 229, 230, 306
SchI GAGTC 3 cut(s) 280, 380, 557
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 5 cut(s) 233, 252, 528, 537, 686
SetI ASST 9 cut(s) 144, 268, 311, 408, 417, 489, 498, 516, 683
SfaNI GCATC 1 cut(s) 71
SfcI CTRYAG 2 cut(s) 622, 699
SfoI GGCGCC 1 cut(s) 478
SinI GGWCC 3 cut(s) 142, 196, 306
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 1 cut(s) 29
SsiI CCGC 7 cut(s) 54, 219, 292, 301, 459, 507, 570
SspDI GGCGCC 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 3 cut(s) 340, 444, 529
TaaI ACNGT 3 cut(s) 196, 523, 708
TaiI ACGT 2 cut(s) 268, 683
TaqI TCGA 1 cut(s) 335
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 2 cut(s) 424, 528
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 172, 240, 493
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 516, 678, 680
TspGWI ACGGA 2 cut(s) 270, 621
TspRI CASTG 2 cut(s) 424, 528
VpaK11BI GGWCC 3 cut(s) 142, 196, 306
XmnI GAANNNNTTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.