Rmu_sc0009474.1_g000016

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009474.1
OG0003557
Physical Location & Seq
Reverse (-)
58307 .. 59020
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009474.1_g000016.1.cds

Sequence Viewer

Length: 714 bp
atgttctcatcctcatggttgaaattaccgagcaaggtgaaccaacccgcaccagcaccgctttcttgcaaaagtatttcttgctcatccttcagaggcatccaaaaggaccaaaacctctgcaaatctcaacccgaaaccgaggtcctgagcccgaggagtgcctccattttgcagcgtatcaagatgtccaccacggtcctcctgcgcctatgggctcgcggcgtagagggcccaagtggctgcaaagtgcgcaacggagaccaacgtgtagtgatttactacacgagtctccgtgtggtgcggaggacctttgaggattgcagcgtggtcagatcgatcctcggagggtttcgggtccccattgacgagcgagatctgtcaatggactccaagtttgtgcaggagctggaggaggtcactgggagcaaggacttgaacttgcccatggtttttgtcagcggagactacattggtggagccgaggaggtgaggcagctccatgaaagcggagagctaaagacaatgctccaagggctcccggtggtggagtccaaaacttgtgatgtgtgcggagggctgagatttattctctgccagacttgcaacggaagccacaaactctacagacagaaagatgggttttgcccttgtatggactgcaacatgaatggcatgattacgtgcccttcatgttcaactgcgggacagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

237

Amino Acids

26.18

Weight (kDa)

8.52

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 254
AccII CGCG 1 cut(s) 222
AciI CCGC 8 cut(s) 48, 59, 222, 304, 462, 510, 573, 704
AclWI GGATC 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 76
AfiI CCNNNNNNNGG 2 cut(s) 547, 655
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 3 cut(s) 22, 439, 699
AluBI AGCT 3 cut(s) 409, 499, 517
AluI AGCT 3 cut(s) 409, 499, 517
Alw26I GTCTC 3 cut(s) 255, 296, 459
AlwI GGATC 1 cut(s) 334
AlwNI CAGNNNCTG 1 cut(s) 409
Ama87I CYCGRG 1 cut(s) 154
AoxI GGCC 1 cut(s) 232
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 4 cut(s) 175, 243, 324, 496
ArsI GACNNNNNNTTYG 4 cut(s) 129, 161, 380, 412
AspLEI GCGC 2 cut(s) 210, 255
AspS9I GGNCC 7 cut(s) 109, 145, 199, 232, 233, 309, 358
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 2 cut(s) 49, 502
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 5 cut(s) 109, 145, 199, 309, 358
BaeGI GKGCMC 2 cut(s) 236, 689
BanII GRGCYC 4 cut(s) 155, 220, 236, 540
BauI CACGAG 1 cut(s) 286
BbvI GCAGC 4 cut(s) 187, 230, 336, 508
BccI CCATC 1 cut(s) 632
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 3 cut(s) 255, 296, 459
BfmI CTRYAG 1 cut(s) 625
BglI GCCNNNNNGGC 1 cut(s) 240
BglII AGATCT 1 cut(s) 376
BisI GCNGC 5 cut(s) 176, 223, 244, 325, 497
BlsI GCNGC 5 cut(s) 177, 224, 245, 326, 498
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 5 cut(s) 109, 145, 199, 309, 358
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 7 cut(s) 109, 145, 199, 232, 233, 309, 358
BmiI GGNNCC 5 cut(s) 234, 359, 360, 481, 539
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 431
Bpu10I CCTNAGC 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 542
Bsa29I ATCGAT 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 7 cut(s) 141, 155, 195, 343, 447, 483, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 655
Bse1I ACTGG 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 338
BseDI CCNNGG 7 cut(s) 141, 155, 195, 343, 447, 483, 532
BseGI GGATG 3 cut(s) 8, 86, 99
BseLI CCNNNNNNNGG 2 cut(s) 547, 655
BseMII CTCAG 2 cut(s) 140, 572
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 3 cut(s) 172, 428, 500
BseSI GKGCMC 2 cut(s) 236, 689
BseXI GCAGC 4 cut(s) 187, 230, 336, 508
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 547, 655
BsmAI GTCTC 3 cut(s) 255, 296, 459
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 1 cut(s) 234
Bso31I GGTCTC 1 cut(s) 255
BsoBI CYCGRG 1 cut(s) 154
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 5 cut(s) 155, 220, 236, 540, 689
Bsp143I GATC 3 cut(s) 335, 339, 376
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 8 cut(s) 48, 59, 222, 304, 462, 510, 573, 704
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 141, 573
BspDI ATCGAT 1 cut(s) 338
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 5 cut(s) 234, 359, 360, 481, 539
BspPI GGATC 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 255
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 7 cut(s) 141, 155, 195, 343, 447, 483, 532
BssMI GATC 3 cut(s) 335, 339, 376
BssSI CACGAG 1 cut(s) 286
BssT1I CCWWGG 2 cut(s) 447, 532
Bst2BI CACGAG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 199, 711
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 149, 581
BstDSI CCRYGG 2 cut(s) 195, 447
BstF5I GGATG 3 cut(s) 8, 86, 99
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 2 cut(s) 210, 255
BstKTI GATC 3 cut(s) 338, 342, 379
BstMAI GTCTC 3 cut(s) 255, 296, 459
BstMBI GATC 3 cut(s) 335, 339, 376
BstMWI GCNNNNNNNGC 6 cut(s) 231, 240, 252, 535, 603, 612
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 625
BstSLI GKGCMC 2 cut(s) 236, 689
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 187, 230, 336, 508
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 338
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 338
BtgI CCRYGG 2 cut(s) 195, 447
BtsCI GGATG 3 cut(s) 8, 86, 99
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 220
CaiI CAGNNNCTG 1 cut(s) 409
CfoI GCGC 2 cut(s) 210, 255
Cfr13I GGNCC 7 cut(s) 109, 145, 199, 232, 233, 309, 358
ClaI ATCGAT 1 cut(s) 338
CviAII CATG 6 cut(s) 15, 448, 503, 667, 676, 693
DdeI CTNAG 2 cut(s) 149, 581
DpnI GATC 3 cut(s) 337, 341, 378
DpnII GATC 3 cut(s) 335, 339, 376
Eco130I CCWWGG 2 cut(s) 447, 532
Eco24I GRGCYC 4 cut(s) 155, 220, 236, 540
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 5 cut(s) 109, 145, 199, 309, 358
Eco57I CTGAAG 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 154
EcoO109I RGGNCCY 4 cut(s) 145, 232, 309, 358
EcoT14I CCWWGG 2 cut(s) 447, 532
EcoT38I GRGCYC 4 cut(s) 155, 220, 236, 540
ErhI CCWWGG 2 cut(s) 447, 532
FaeI CATG 6 cut(s) 18, 451, 506, 670, 679, 696
FaiI YATR 8 cut(s) 16, 214, 449, 504, 656, 668, 677, 694
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FaqI GGGAC 1 cut(s) 344
FatI CATG 6 cut(s) 14, 447, 502, 666, 675, 692
FauI CCCGC 2 cut(s) 55, 697
Fnu4HI GCNGC 5 cut(s) 176, 223, 244, 325, 497
FokI GGATG 2 cut(s) 73, 86
FriOI GRGCYC 4 cut(s) 155, 220, 236, 540
Fsp4HI GCNGC 5 cut(s) 176, 223, 244, 325, 497
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 2 cut(s) 209, 254
GluI GCNGC 5 cut(s) 176, 223, 244, 325, 497
GsuI CTGGAG 1 cut(s) 431
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 542
HhaI GCGC 2 cut(s) 210, 255
Hin1II CATG 6 cut(s) 18, 451, 506, 670, 679, 696
Hin6I GCGC 2 cut(s) 208, 253
HinP1I GCGC 2 cut(s) 208, 253
HinfI GANTC 3 cut(s) 289, 389, 551
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 49, 502
Hpy166II GTNNAC 2 cut(s) 40, 192
Hpy188I TCNGA 3 cut(s) 95, 335, 347
Hpy188III TCNNGA 2 cut(s) 148, 184
Hpy8I GTNNAC 2 cut(s) 40, 192
HpyAV CCTTC 2 cut(s) 100, 699
HpyCH4III ACNGT 2 cut(s) 199, 711
HpyCH4IV ACGT 2 cut(s) 268, 683
HpyCH4V TGCA 8 cut(s) 69, 123, 175, 246, 324, 403, 606, 663
HpyF10VI GCNNNNNNNGC 6 cut(s) 231, 240, 252, 535, 603, 612
HpyF3I CTNAG 2 cut(s) 149, 581
HpySE526I ACGT 2 cut(s) 268, 683
Hsp92II CATG 6 cut(s) 18, 451, 506, 670, 679, 696
HspAI GCGC 2 cut(s) 208, 253
KflI GGGWCCC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 335, 339, 376
LmnI GCTCC 6 cut(s) 406, 426, 479, 504, 534, 543
LpnPI CCDG 8 cut(s) 66, 161, 218, 389, 395, 408, 555, 611
Lsp1109I GCAGC 4 cut(s) 187, 230, 336, 508
LweI GCATC 1 cut(s) 108
MaeII ACGT 2 cut(s) 268, 683
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 337, 341, 378
MboI GATC 3 cut(s) 335, 339, 376
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 5 cut(s) 155, 220, 236, 540, 689
MluCI AATT 1 cut(s) 23
MlyI GAGTC 3 cut(s) 298, 383, 560
MslI CAYNNNNRTG 1 cut(s) 13
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 6 cut(s) 231, 240, 252, 535, 603, 612
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 1 cut(s) 447
NdeII GATC 3 cut(s) 335, 339, 376
NlaIII CATG 6 cut(s) 18, 451, 506, 670, 679, 696
NlaIV GGNNCC 5 cut(s) 234, 359, 360, 481, 539
NmeAIII GCCGAG 1 cut(s) 508
NmuCI GTSAC 1 cut(s) 418
NsbI TGCGCA 1 cut(s) 254
PkrI GCNGC 5 cut(s) 177, 224, 245, 326, 498
PleI GAGTC 3 cut(s) 297, 383, 559
PpsI GAGTC 3 cut(s) 297, 383, 559
Ppu21I YACGTR 1 cut(s) 684
PpuMI RGGWCCY 3 cut(s) 145, 309, 358
Psp5II RGGWCCY 3 cut(s) 145, 309, 358
PspN4I GGNNCC 5 cut(s) 234, 359, 360, 481, 539
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 7 cut(s) 109, 145, 199, 232, 233, 309, 358
PspPPI RGGWCCY 3 cut(s) 145, 309, 358
PstNI CAGNNNCTG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 13
SatI GCNGC 5 cut(s) 176, 223, 244, 325, 497
Sau3AI GATC 3 cut(s) 335, 339, 376
Sau96I GGNCC 7 cut(s) 109, 145, 199, 232, 233, 309, 358
SchI GAGTC 3 cut(s) 298, 383, 560
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 5 cut(s) 155, 220, 236, 540, 689
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 625
SinI GGWCC 5 cut(s) 109, 145, 199, 309, 358
SmiMI CAYNNNNRTG 1 cut(s) 13
Sse9I AATT 1 cut(s) 23
SsiI CCGC 8 cut(s) 48, 59, 222, 304, 462, 510, 573, 704
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 2 cut(s) 447, 532
TaaI ACNGT 2 cut(s) 199, 711
TaiI ACGT 2 cut(s) 271, 686
TaqI TCGA 1 cut(s) 338
TasI AATT 1 cut(s) 23
TauI GCSGC 1 cut(s) 225
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 4 cut(s) 175, 243, 324, 496
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 519, 681, 683
TspGWI ACGGA 3 cut(s) 273, 284, 624
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 5 cut(s) 109, 145, 199, 309, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.