Rroxscaffold_4G00286190

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003557
Physical Location & Seq
Forward (+)
7315327 .. 7316031
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286190.1

Sequence Viewer

Length: 705 bp
ATGTTTGCACCATCATGGTTGAATTTGAAATCACCAAGCAAGGTGGGAACAATCAAAACACCGCGATCACGTAGACGATTTTCTTGCTCGTCCTTTAAAGACATCCGGAGCTTGTTCCAACCCGAACCTGAAGCCCTCACCCCGAGGAGAGCCTCTATCATACATCGGGCCAAGACATCCACCACAGTCTTTCGCTTGTGGGCCCACCACATTGCGCGCCCAAGTAGGATACACGACGATGATCGCCGTGTGGTAATCTACTATACGAGCCTACGTGTAGTGCGCAGAACTTACGAGGATTGTAAGGTCGTCCGATCAATTTTTAGAGGATTTTGTGTTCCCATAGACGAACGAGATTTGTCAATGGACACAAAATTTTTCGAGGAGTTGCAAGAGCTTATGGGAAGTAGGACCTTCACTTTGCCTATGGTCTTCATCAGTGGGATATGCATCGGTGGGGTTGAAGAGATCCGACAACTCCATGAAAGTGGTGATCTGAAGGTAATGATGCAATGGCTACCGGTGGTAGACTCTAGACCGTGTGACTTATGTGGAGGAATCAAGTTTGTGCTATGCCAAACATGTGATGGAAGTCATAGGGTTCATTGTGACGGTGAATTTAGTCCTTGTACAACTTGTAATGTGAATGGTCTAGTTATGTGTCCGTCATGTTCTACCATGCGGCGCCGAACCTCACCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

234

Amino Acids

26.83

Weight (kDa)

9.23

Isoelectric Point (pI)

66.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 85 - 148 2.7e-09 Glutaredoxin
GRXCR1-2_C PF23733 181 - 225 9.7e-15 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 684
AccI GTMKAC 2 cut(s) 73, 528
AccII CGCG 2 cut(s) 64, 217
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 2 cut(s) 62, 682
AclWI GGATC 1 cut(s) 463
AcsI RAATTY 3 cut(s) 22, 374, 617
AcuI CTGAAG 2 cut(s) 150, 518
AcyI GRCGYC 1 cut(s) 685
AfaI GTAC 1 cut(s) 631
AflIII ACRYGT 2 cut(s) 274, 581
AgeI ACCGGT 1 cut(s) 520
AgsI TTSAA 3 cut(s) 22, 28, 464
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 2 cut(s) 111, 397
AluI AGCT 2 cut(s) 111, 397
AlwI GGATC 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 2 cut(s) 168, 201
ApaI GGGCCC 1 cut(s) 205
ApoI RAATTY 3 cut(s) 22, 374, 617
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AsiGI ACCGGT 1 cut(s) 520
AspLEI GCGC 4 cut(s) 217, 219, 285, 687
AspS9I GGNCC 4 cut(s) 168, 201, 202, 411
AsuHPI GGTGA 5 cut(s) 24, 130, 503, 626, 687
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 684
BanII GRGCYC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 424
BccI CCATC 2 cut(s) 19, 581
BceAI ACGGC 1 cut(s) 231
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciVI GTATCC 1 cut(s) 222
BfaI CTAG 2 cut(s) 534, 653
BfoI RGCGCY 1 cut(s) 688
BfuI GTATCC 1 cut(s) 222
BisI GCNGC 1 cut(s) 683
BlsI GCNGC 1 cut(s) 684
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 4 cut(s) 168, 201, 202, 411
BmiI GGNNCC 2 cut(s) 203, 686
BmsI GCATC 2 cut(s) 459, 498
BpiI GAAGAC 1 cut(s) 424
BsaAI YACGTR 2 cut(s) 71, 275
BsaBI GATNNNNATC 1 cut(s) 449
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 2 cut(s) 105, 520
Bse118I RCCGGY 1 cut(s) 520
Bse3DI GCAATG 2 cut(s) 210, 518
Bse8I GATNNNNATC 1 cut(s) 449
BseAI TCCGGA 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 102, 176
BseJI GATNNNNATC 1 cut(s) 449
BseMI GCAATG 2 cut(s) 210, 518
BsePI GCGCGC 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 160, 398
BseSI GKGCMC 1 cut(s) 205
Bsh1236I CGCG 2 cut(s) 64, 217
BshFI GGCC 2 cut(s) 170, 203
BshNI GGYRCC 1 cut(s) 684
BshTI ACCGGT 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 106, 521
BsnI GGCC 2 cut(s) 170, 203
BsoBI CYCGRG 1 cut(s) 142
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 205
Bsp13I TCCGGA 1 cut(s) 105
Bsp1407I TGTACA 1 cut(s) 629
Bsp143I GATC 5 cut(s) 65, 241, 314, 468, 493
BspACI CCGC 2 cut(s) 62, 682
BspANI GGCC 2 cut(s) 170, 203
BspEI TCCGGA 1 cut(s) 105
BspFNI CGCG 2 cut(s) 64, 217
BspLI GGNNCC 2 cut(s) 203, 686
BspPI GGATC 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 684
BsrDI GCAATG 2 cut(s) 210, 518
BsrFI RCCGGY 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 629
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 143
BssHII GCGCGC 1 cut(s) 215
BssMI GATC 5 cut(s) 65, 241, 314, 468, 493
BssNI GRCGYC 1 cut(s) 685
Bst4CI ACNGT 4 cut(s) 187, 540, 614, 702
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 629
BstBAI YACGTR 2 cut(s) 71, 275
BstC8I GCNNGC 1 cut(s) 217
BstF5I GGATG 2 cut(s) 102, 176
BstFNI CGCG 2 cut(s) 64, 217
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 4 cut(s) 217, 219, 285, 687
BstKTI GATC 5 cut(s) 68, 244, 317, 471, 496
BstMBI GATC 5 cut(s) 65, 241, 314, 468, 493
BstNSI RCATGY 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 2 cut(s) 64, 217
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 468
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 1 cut(s) 468
BsuI GTATCC 1 cut(s) 222
BsuRI GGCC 2 cut(s) 170, 203
BtsCI GGATG 2 cut(s) 102, 176
BtsIMutI CAGTG 2 cut(s) 445, 698
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 4 cut(s) 217, 219, 285, 687
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 4 cut(s) 168, 201, 202, 411
Csp6I GTAC 1 cut(s) 630
CspAI ACCGGT 1 cut(s) 520
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 5 cut(s) 15, 482, 582, 669, 679
CviJI RGCY 8 cut(s) 111, 134, 152, 170, 203, 270, 397, 517
CviKI_1 RGCY 8 cut(s) 111, 134, 152, 170, 203, 270, 397, 517
CviQI GTAC 1 cut(s) 630
DinI GGCGCC 1 cut(s) 686
DpnI GATC 5 cut(s) 67, 243, 316, 470, 495
DpnII GATC 5 cut(s) 65, 241, 314, 468, 493
DraI TTTAAA 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 411
Eco57I CTGAAG 2 cut(s) 150, 518
Eco88I CYCGRG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 411
EcoT22I ATGCAT 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 686
EheI GGCGCC 1 cut(s) 686
FaeI CATG 5 cut(s) 18, 485, 585, 672, 682
FatI CATG 5 cut(s) 14, 481, 581, 668, 678
FblI GTMKAC 2 cut(s) 73, 528
Fnu4HI GCNGC 1 cut(s) 683
FokI GGATG 2 cut(s) 89, 163
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 1 cut(s) 683
FspBI CTAG 2 cut(s) 534, 653
FspI TGCGCA 1 cut(s) 284
GlaI GCGC 4 cut(s) 216, 218, 284, 686
GluI GCNGC 1 cut(s) 683
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 2 cut(s) 170, 203
HapII CCGG 2 cut(s) 106, 521
HhaI GCGC 4 cut(s) 217, 219, 285, 687
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 5 cut(s) 18, 485, 585, 672, 682
Hin6I GCGC 4 cut(s) 215, 217, 283, 685
HinP1I GCGC 4 cut(s) 215, 217, 283, 685
HinfI GANTC 2 cut(s) 530, 558
HpaII CCGG 2 cut(s) 106, 521
HphI GGTGA 5 cut(s) 24, 130, 503, 626, 687
Hpy166II GTNNAC 2 cut(s) 74, 529
Hpy188I TCNGA 3 cut(s) 314, 473, 498
Hpy188III TCNNGA 2 cut(s) 106, 534
Hpy8I GTNNAC 2 cut(s) 74, 529
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 424, 493
HpyCH4III ACNGT 4 cut(s) 187, 540, 614, 702
HpyCH4IV ACGT 2 cut(s) 70, 274
HpyCH4V TGCA 4 cut(s) 8, 391, 450, 511
HpySE526I ACGT 2 cut(s) 70, 274
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 5 cut(s) 18, 485, 585, 672, 682
HspAI GCGC 4 cut(s) 215, 217, 283, 685
KasI GGCGCC 1 cut(s) 684
Kpn2I TCCGGA 1 cut(s) 105
Kzo9I GATC 5 cut(s) 65, 241, 314, 468, 493
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 3 cut(s) 119, 141, 534
LweI GCATC 2 cut(s) 459, 498
MaeI CTAG 2 cut(s) 534, 653
MaeII ACGT 2 cut(s) 70, 274
MaeIII GTNAC 2 cut(s) 542, 608
MalI GATC 5 cut(s) 67, 243, 316, 470, 495
MboI GATC 5 cut(s) 65, 241, 314, 468, 493
MboII GAAGA 2 cut(s) 424, 476
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 4 cut(s) 22, 318, 374, 617
Mly113I GGCGCC 1 cut(s) 685
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 142, 496
MnlI CCTC 8 cut(s) 138, 146, 163, 289, 320, 376, 548, 703
Mph1103I ATGCAT 1 cut(s) 452
MroI TCCGGA 1 cut(s) 105
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 3 cut(s) 13, 237, 486
MspI CCGG 2 cut(s) 106, 521
MvnI CGCG 2 cut(s) 64, 217
NarI GGCGCC 1 cut(s) 685
NdeII GATC 5 cut(s) 65, 241, 314, 468, 493
NlaIII CATG 5 cut(s) 18, 485, 585, 672, 682
NlaIV GGNNCC 2 cut(s) 203, 686
NmuCI GTSAC 2 cut(s) 542, 608
NsbI TGCGCA 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 1 cut(s) 585
PauI GCGCGC 1 cut(s) 215
PciI ACATGT 1 cut(s) 581
PfeI GAWTC 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 520
PkrI GCNGC 1 cut(s) 684
PleI GAGTC 1 cut(s) 524
PluTI GGCGCC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 524
Ppu21I YACGTR 2 cut(s) 71, 275
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 411
PspN4I GGNNCC 2 cut(s) 203, 686
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 4 cut(s) 168, 201, 202, 411
PspPPI RGGWCCY 1 cut(s) 411
PsuI RGATCY 1 cut(s) 468
PteI GCGCGC 1 cut(s) 215
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
RseI CAYNNNNRTG 3 cut(s) 13, 237, 486
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 1 cut(s) 683
Sau3AI GATC 5 cut(s) 65, 241, 314, 468, 493
Sau96I GGNCC 4 cut(s) 168, 201, 202, 411
SchI GAGTC 1 cut(s) 524
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 2 cut(s) 459, 498
SfoI GGCGCC 1 cut(s) 686
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 3 cut(s) 13, 237, 486
Sse9I AATT 4 cut(s) 22, 318, 374, 617
SsiI CCGC 2 cut(s) 62, 682
SspDI GGCGCC 1 cut(s) 684
SspMI CTAG 2 cut(s) 534, 653
TaaI ACNGT 4 cut(s) 187, 540, 614, 702
TaiI ACGT 2 cut(s) 73, 277
TaqI TCGA 1 cut(s) 381
TasI AATT 4 cut(s) 22, 318, 374, 617
TatI WGTACW 1 cut(s) 629
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 1 cut(s) 558
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 445, 705
TseFI GTSAC 2 cut(s) 542, 608
Tsp45I GTSAC 2 cut(s) 542, 608
TspDTI ATGAA 3 cut(s) 424, 498, 593
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 2 cut(s) 445, 705
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 3 cut(s) 22, 374, 617
XbaI TCTAGA 1 cut(s) 533
XceI RCATGY 1 cut(s) 585
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmiI GTMKAC 2 cut(s) 73, 528
XspI CTAG 2 cut(s) 534, 653
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.