Rh1AG369600

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003557
Physical Location & Seq
Reverse (-)
60353441 .. 60354160
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG369600.1

Sequence Viewer

Length: 720 bp
ATGGCCATGTTCTCATCCTCATGGTTGAAATTACCGAGCAAGGTGAACCAACCCGCGCCAGCACCGCGTTCGTGCAAAAGTATTTCTTGCTCATCCTTCAGAGACATCCAAAAGGACCAAAACCTTTGCAAATCTCAACCCGAAACCGAGGTCCTGAGCCCGAGGAGTGCCTCCATTTTGCAGCGTATCAAGATGTCCACCACGGTCCTCCTGCGCCTCTGGGCTCGCGGCGTAGAGGGCCCAAGTGGCTGCAAAGTGCTCAACGGAGACCAACGTGTAGTGATTTACTACACGAGTCTCCGTGTGGTGCGGAGGACCTTTGAGGATTGCAGGGTGGTCAGATCGATCCTCGGAGGGTTTCGGGTCCCCATTGACGAGCGAGATCTGTCAATGGACTCCAAGTTTGTGCAGGAGCTGGAGGAGGTCACTGGGAGCAAGGACTTGAACTTGCCCATGGTTTTCGTCAGCGGATACTACATTGGTGGAGCCGAGGAGGTGAGGCAGCTCCATGAAAGCGGAGAGCTAAAGACAATGCTCCAAGGGCTCCCGGTGGTGGAGTCCAAAACTTGTGATGTGTGCGGAGGGCTGAGATTTATTCTCTGCCAGACTTGCAACGGAAGCCACAAACTCTACAGACAGAAAGATGGGTTTTGCCCTTGTATGGACTGCAACATGAATGGCATGATTACGTGCCCTTCATGTTCAACTGCGGGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

239

Amino Acids

26.55

Weight (kDa)

8.64

Isoelectric Point (pI)

58.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 94 - 158 3.3e-08 Glutaredoxin
GRXCR1-2_C PF23733 190 - 235 2.9e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 56, 67, 228
AciI CCGC 8 cut(s) 54, 65, 228, 310, 468, 516, 579, 710
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 553, 661
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 3 cut(s) 28, 445, 705
AluBI AGCT 3 cut(s) 415, 505, 523
AluI AGCT 3 cut(s) 415, 505, 523
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 3 cut(s) 96, 261, 302
AlwI GGATC 1 cut(s) 340
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 2 cut(s) 3, 238
ApaI GGGCCC 1 cut(s) 242
ApeKI GCWGC 3 cut(s) 181, 249, 502
ArsI GACNNNNNNTTYG 4 cut(s) 135, 167, 386, 418
AspLEI GCGC 2 cut(s) 58, 216
AspS9I GGNCC 7 cut(s) 115, 151, 205, 238, 239, 315, 364
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 2 cut(s) 55, 508
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 5 cut(s) 115, 151, 205, 315, 364
BaeGI GKGCMC 2 cut(s) 242, 695
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 4 cut(s) 161, 226, 242, 546
BauI CACGAG 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 3 cut(s) 193, 236, 514
BccI CCATC 1 cut(s) 638
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BciVI GTATCC 1 cut(s) 464
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 3 cut(s) 96, 261, 302
BfmI CTRYAG 1 cut(s) 631
BfuI GTATCC 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 246
BglII AGATCT 1 cut(s) 382
BisI GCNGC 4 cut(s) 182, 229, 250, 503
BlsI GCNGC 4 cut(s) 183, 230, 251, 504
Bme1390I CCNGG 1 cut(s) 548
Bme18I GGWCC 5 cut(s) 115, 151, 205, 315, 364
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 7 cut(s) 115, 151, 205, 238, 239, 315, 364
BmiI GGNNCC 5 cut(s) 240, 365, 366, 487, 545
BmrFI CCNGG 1 cut(s) 548
BmrI ACTGGG 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 548
Bsa29I ATCGAT 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 7 cut(s) 147, 161, 201, 349, 453, 489, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 553, 661
Bse1I ACTGG 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 344
BseDI CCNNGG 7 cut(s) 147, 161, 201, 349, 453, 489, 538
BseGI GGATG 3 cut(s) 14, 92, 105
BseLI CCNNNNNNNGG 2 cut(s) 553, 661
BseMII CTCAG 2 cut(s) 146, 578
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 178, 434, 506
BseSI GKGCMC 2 cut(s) 242, 695
BseXI GCAGC 3 cut(s) 193, 236, 514
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 3 cut(s) 56, 67, 228
BshFI GGCC 2 cut(s) 5, 240
BshVI ATCGAT 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 553, 661
BsmAI GTCTC 3 cut(s) 96, 261, 302
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 5, 240
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 160
Bsp120I GGGCCC 1 cut(s) 238
Bsp1286I GDGCHC 6 cut(s) 161, 226, 242, 261, 546, 695
Bsp143I GATC 3 cut(s) 341, 345, 382
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 8 cut(s) 54, 65, 228, 310, 468, 516, 579, 710
BspANI GGCC 2 cut(s) 5, 240
BspCNI CTCAG 2 cut(s) 147, 579
BspDI ATCGAT 1 cut(s) 344
BspFNI CGCG 3 cut(s) 56, 67, 228
BspLI GGNNCC 5 cut(s) 240, 365, 366, 487, 545
BspPI GGATC 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 261
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 7 cut(s) 147, 161, 201, 349, 453, 489, 538
BssMI GATC 3 cut(s) 341, 345, 382
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 2 cut(s) 453, 538
Bst2BI CACGAG 1 cut(s) 292
Bst4CI ACNGT 2 cut(s) 205, 717
BstBAI YACGTR 1 cut(s) 690
BstC8I GCNNGC 2 cut(s) 60, 226
BstDEI CTNAG 2 cut(s) 155, 587
BstDSI CCRYGG 2 cut(s) 201, 453
BstF5I GGATG 3 cut(s) 14, 92, 105
BstFNI CGCG 3 cut(s) 56, 67, 228
BstHHI GCGC 2 cut(s) 58, 216
BstKTI GATC 3 cut(s) 344, 348, 385
BstMAI GTCTC 3 cut(s) 96, 261, 302
BstMBI GATC 3 cut(s) 341, 345, 382
BstMWI GCNNNNNNNGC 6 cut(s) 64, 237, 246, 541, 609, 618
BstSCI CCNGG 1 cut(s) 546
BstSFI CTRYAG 1 cut(s) 631
BstSLI GKGCMC 2 cut(s) 242, 695
BstUI CGCG 3 cut(s) 56, 67, 228
BstV1I GCAGC 3 cut(s) 193, 236, 514
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 344
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 2 cut(s) 5, 240
BsuTUI ATCGAT 1 cut(s) 344
BtgI CCRYGG 2 cut(s) 201, 453
BtsCI GGATG 3 cut(s) 14, 92, 105
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 60, 226
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 2 cut(s) 58, 216
Cfr13I GGNCC 7 cut(s) 115, 151, 205, 238, 239, 315, 364
ClaI ATCGAT 1 cut(s) 344
CviAII CATG 7 cut(s) 7, 21, 454, 509, 673, 682, 699
DdeI CTNAG 2 cut(s) 155, 587
DpnI GATC 3 cut(s) 343, 347, 384
DpnII GATC 3 cut(s) 341, 345, 382
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 453, 538
Eco24I GRGCYC 4 cut(s) 161, 226, 242, 546
Eco31I GGTCTC 1 cut(s) 261
Eco47I GGWCC 5 cut(s) 115, 151, 205, 315, 364
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 160
EcoO109I RGGNCCY 4 cut(s) 151, 238, 315, 364
EcoT14I CCWWGG 2 cut(s) 453, 538
EcoT38I GRGCYC 4 cut(s) 161, 226, 242, 546
ErhI CCWWGG 2 cut(s) 453, 538
FaeI CATG 7 cut(s) 10, 24, 457, 512, 676, 685, 702
FaiI YATR 8 cut(s) 8, 22, 455, 510, 662, 674, 683, 700
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FaqI GGGAC 1 cut(s) 350
FatI CATG 7 cut(s) 6, 20, 453, 508, 672, 681, 698
FauI CCCGC 2 cut(s) 61, 703
Fnu4HI GCNGC 4 cut(s) 182, 229, 250, 503
FokI GGATG 2 cut(s) 79, 92
FriOI GRGCYC 4 cut(s) 161, 226, 242, 546
Fsp4HI GCNGC 4 cut(s) 182, 229, 250, 503
GlaI GCGC 2 cut(s) 57, 215
GluI GCNGC 4 cut(s) 182, 229, 250, 503
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 2 cut(s) 5, 240
HapII CCGG 1 cut(s) 548
HhaI GCGC 2 cut(s) 58, 216
Hin1II CATG 7 cut(s) 10, 24, 457, 512, 676, 685, 702
Hin6I GCGC 2 cut(s) 56, 214
HinP1I GCGC 2 cut(s) 56, 214
HinfI GANTC 3 cut(s) 295, 395, 557
HpaII CCGG 1 cut(s) 548
HphI GGTGA 2 cut(s) 55, 508
Hpy166II GTNNAC 2 cut(s) 46, 198
Hpy188I TCNGA 3 cut(s) 101, 341, 353
Hpy188III TCNNGA 2 cut(s) 154, 190
Hpy8I GTNNAC 2 cut(s) 46, 198
HpyAV CCTTC 2 cut(s) 106, 705
HpyCH4III ACNGT 2 cut(s) 205, 717
HpyCH4IV ACGT 2 cut(s) 274, 689
HpyCH4V TGCA 8 cut(s) 75, 129, 181, 252, 330, 409, 612, 669
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 237, 246, 541, 609, 618
HpyF3I CTNAG 2 cut(s) 155, 587
HpySE526I ACGT 2 cut(s) 274, 689
Hsp92II CATG 7 cut(s) 10, 24, 457, 512, 676, 685, 702
HspAI GCGC 2 cut(s) 56, 214
KflI GGGWCCC 1 cut(s) 364
Kzo9I GATC 3 cut(s) 341, 345, 382
LmnI GCTCC 6 cut(s) 412, 432, 485, 510, 540, 549
Lsp1109I GCAGC 3 cut(s) 193, 236, 514
MaeII ACGT 2 cut(s) 274, 689
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 343, 347, 384
MboI GATC 3 cut(s) 341, 345, 382
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 6 cut(s) 161, 226, 242, 261, 546, 695
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 304, 389, 566
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 468
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 548
MvnI CGCG 3 cut(s) 56, 67, 228
MwoI GCNNNNNNNGC 6 cut(s) 64, 237, 246, 541, 609, 618
NciI CCSGG 1 cut(s) 548
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 341, 345, 382
NlaIII CATG 7 cut(s) 10, 24, 457, 512, 676, 685, 702
NlaIV GGNNCC 5 cut(s) 240, 365, 366, 487, 545
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 424
PkrI GCNGC 4 cut(s) 183, 230, 251, 504
PleI GAGTC 3 cut(s) 303, 389, 565
PpsI GAGTC 3 cut(s) 303, 389, 565
Ppu21I YACGTR 1 cut(s) 690
PpuMI RGGWCCY 3 cut(s) 151, 315, 364
Psp5II RGGWCCY 3 cut(s) 151, 315, 364
PspN4I GGNNCC 5 cut(s) 240, 365, 366, 487, 545
PspOMI GGGCCC 1 cut(s) 238
PspPI GGNCC 7 cut(s) 115, 151, 205, 238, 239, 315, 364
PspPPI RGGWCCY 3 cut(s) 151, 315, 364
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 19
SatI GCNGC 4 cut(s) 182, 229, 250, 503
Sau3AI GATC 3 cut(s) 341, 345, 382
Sau96I GGNCC 7 cut(s) 115, 151, 205, 238, 239, 315, 364
SchI GAGTC 3 cut(s) 304, 389, 566
ScrFI CCNGG 1 cut(s) 548
SduI GDGCHC 6 cut(s) 161, 226, 242, 261, 546, 695
SfcI CTRYAG 1 cut(s) 631
SinI GGWCC 5 cut(s) 115, 151, 205, 315, 364
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 1 cut(s) 29
SsiI CCGC 8 cut(s) 54, 65, 228, 310, 468, 516, 579, 710
StyD4I CCNGG 1 cut(s) 546
StyI CCWWGG 2 cut(s) 453, 538
TaaI ACNGT 2 cut(s) 205, 717
TaiI ACGT 2 cut(s) 277, 692
TaqI TCGA 1 cut(s) 344
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 231
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 181, 249, 502
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 525, 687, 689
TspGWI ACGGA 3 cut(s) 279, 290, 630
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 5 cut(s) 115, 151, 205, 315, 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.