Rw1G032530

At5g39865-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003557
Physical Location & Seq
Reverse (-)
60763385 .. 60764089
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032530.1

Sequence Viewer

Length: 705 bp
ATGTTTGCACCATCATGGTTGAATTTGAAATCACCAAGCAAGGTGGGAACAATCAAAACACCGCGATCACGTAGACGATTTTCTTGCTCGTCCTTTAAAGACATCCGGAGCTTGTTCCAACCCGAACCTGAAGCCCTCACCCCGAGGAGAGCTTCTATCATACATCGGGCCAAGACATCCACCACAGTCTTTCGCTTGTGGGCCCACCACATTGCGCGCCCAAGTAGGATACACGACGATGATCGCGGTGTGGTAATCTATTATACGAGCCTACGTGTAGTGCGCAGAACTTACGAGGATTGTAAGGTTGTCCGATCAATTTTTAGAGGATTTTGTGTTCCCATAGACGAACGAGATTTGTCAATGGACACAAAATTTTTCGAGGAGTTGCAAGAGCTTATGGGAAGTAGGACCTTCACTTTGCCTGTGGTCTTCATCAGTGGGATATGCATTGGTGGGGTTGAAGAGATCCGACAACTCCATGAAAGTGGTGATCTGAAGGTAATGATGCAATGGCTACCGGTGGTAGACTCTAGACCGTGTGACTTATGTGGAGGAATCAAGTTTGTGCTATGCCAAACATGTGATGGAAGTCATAGGGTTCATTATGACGGTGAATTTAGTCCTTGTACAACTTGTAATGTGAATGGTCTAGTTATGTGTCCGTCATGTTCTACCATGCGGCTCCGGATCTCACCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

234

Amino Acids

26.72

Weight (kDa)

9.08

Isoelectric Point (pI)

63.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 85 - 148 7.5e-10 Glutaredoxin
GRXCR1-2_C PF23733 181 - 225 6.2e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 284
AccI GTMKAC 2 cut(s) 73, 528
AccII CGCG 3 cut(s) 64, 217, 246
AccIII TCCGGA 2 cut(s) 105, 687
AciI CCGC 3 cut(s) 62, 246, 682
AclWI GGATC 2 cut(s) 463, 698
AcsI RAATTY 3 cut(s) 22, 374, 617
AcuI CTGAAG 2 cut(s) 150, 518
AfaI GTAC 1 cut(s) 631
AflIII ACRYGT 2 cut(s) 274, 581
AgeI ACCGGT 1 cut(s) 520
AgsI TTSAA 3 cut(s) 22, 28, 464
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 3 cut(s) 111, 152, 397
AluI AGCT 3 cut(s) 111, 152, 397
AlwI GGATC 2 cut(s) 463, 698
Ama87I CYCGRG 1 cut(s) 142
Aor13HI TCCGGA 2 cut(s) 105, 687
AoxI GGCC 2 cut(s) 168, 201
ApaI GGGCCC 1 cut(s) 205
ApoI RAATTY 3 cut(s) 22, 374, 617
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AsiGI ACCGGT 1 cut(s) 520
AspLEI GCGC 3 cut(s) 217, 219, 285
AspS9I GGNCC 4 cut(s) 168, 201, 202, 411
AsuHPI GGTGA 5 cut(s) 24, 130, 503, 626, 687
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 424
BccI CCATC 2 cut(s) 19, 581
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciVI GTATCC 1 cut(s) 222
BfaI CTAG 2 cut(s) 534, 653
BfuI GTATCC 1 cut(s) 222
BisI GCNGC 1 cut(s) 683
BlsI GCNGC 1 cut(s) 684
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 4 cut(s) 168, 201, 202, 411
BmiI GGNNCC 2 cut(s) 203, 686
BmsI GCATC 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 424
BsaAI YACGTR 2 cut(s) 71, 275
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 3 cut(s) 105, 520, 687
Bse118I RCCGGY 1 cut(s) 520
Bse3DI GCAATG 2 cut(s) 210, 518
BseAI TCCGGA 2 cut(s) 105, 687
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 102, 176
BseMI GCAATG 2 cut(s) 210, 518
BsePI GCGCGC 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 160, 398
BseSI GKGCMC 1 cut(s) 205
Bsh1236I CGCG 3 cut(s) 64, 217, 246
BshFI GGCC 2 cut(s) 170, 203
BshTI ACCGGT 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 3 cut(s) 106, 521, 688
BsnI GGCC 2 cut(s) 170, 203
BsoBI CYCGRG 1 cut(s) 142
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 205
Bsp13I TCCGGA 2 cut(s) 105, 687
Bsp1407I TGTACA 1 cut(s) 629
Bsp143I GATC 6 cut(s) 65, 241, 314, 468, 493, 690
BspACI CCGC 3 cut(s) 62, 246, 682
BspANI GGCC 2 cut(s) 170, 203
BspEI TCCGGA 2 cut(s) 105, 687
BspFNI CGCG 3 cut(s) 64, 217, 246
BspLI GGNNCC 2 cut(s) 203, 686
BspPI GGATC 2 cut(s) 463, 698
BsrDI GCAATG 2 cut(s) 210, 518
BsrFI RCCGGY 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 629
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 143
BssHII GCGCGC 1 cut(s) 215
BssMI GATC 6 cut(s) 65, 241, 314, 468, 493, 690
Bst4CI ACNGT 3 cut(s) 187, 540, 614
Bst6I CTCTTC 1 cut(s) 459
BstAUI TGTACA 1 cut(s) 629
BstBAI YACGTR 2 cut(s) 71, 275
BstC8I GCNNGC 1 cut(s) 217
BstF5I GGATG 2 cut(s) 102, 176
BstFNI CGCG 3 cut(s) 64, 217, 246
BstHHI GCGC 3 cut(s) 217, 219, 285
BstKTI GATC 6 cut(s) 68, 244, 317, 471, 496, 693
BstMBI GATC 6 cut(s) 65, 241, 314, 468, 493, 690
BstNSI RCATGY 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 3 cut(s) 64, 217, 246
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 2 cut(s) 468, 690
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 2 cut(s) 468, 690
BsuI GTATCC 1 cut(s) 222
BsuRI GGCC 2 cut(s) 170, 203
BtsCI GGATG 2 cut(s) 102, 176
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 3 cut(s) 217, 219, 285
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 4 cut(s) 168, 201, 202, 411
Csp6I GTAC 1 cut(s) 630
CspAI ACCGGT 1 cut(s) 520
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 5 cut(s) 15, 482, 582, 669, 679
CviJI RGCY 9 cut(s) 111, 134, 152, 170, 203, 270, 397, 517, 685
CviKI_1 RGCY 9 cut(s) 111, 134, 152, 170, 203, 270, 397, 517, 685
CviQI GTAC 1 cut(s) 630
DpnI GATC 6 cut(s) 67, 243, 316, 470, 495, 692
DpnII GATC 6 cut(s) 65, 241, 314, 468, 493, 690
DraI TTTAAA 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 411
Eco57I CTGAAG 2 cut(s) 150, 518
Eco88I CYCGRG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 411
EcoT22I ATGCAT 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 5 cut(s) 18, 485, 585, 672, 682
FatI CATG 5 cut(s) 14, 481, 581, 668, 678
FblI GTMKAC 2 cut(s) 73, 528
Fnu4HI GCNGC 1 cut(s) 683
FokI GGATG 2 cut(s) 89, 163
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 1 cut(s) 683
FspBI CTAG 2 cut(s) 534, 653
FspI TGCGCA 1 cut(s) 284
GlaI GCGC 3 cut(s) 216, 218, 284
GluI GCNGC 1 cut(s) 683
HaeIII GGCC 2 cut(s) 170, 203
HapII CCGG 3 cut(s) 106, 521, 688
HhaI GCGC 3 cut(s) 217, 219, 285
Hin1II CATG 5 cut(s) 18, 485, 585, 672, 682
Hin6I GCGC 3 cut(s) 215, 217, 283
HinP1I GCGC 3 cut(s) 215, 217, 283
HinfI GANTC 2 cut(s) 530, 558
HpaII CCGG 3 cut(s) 106, 521, 688
HphI GGTGA 5 cut(s) 24, 130, 503, 626, 687
Hpy166II GTNNAC 2 cut(s) 74, 529
Hpy188I TCNGA 3 cut(s) 314, 473, 498
Hpy188III TCNNGA 3 cut(s) 106, 534, 688
Hpy8I GTNNAC 2 cut(s) 74, 529
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 424, 493
HpyCH4III ACNGT 3 cut(s) 187, 540, 614
HpyCH4IV ACGT 2 cut(s) 70, 274
HpyCH4V TGCA 4 cut(s) 8, 391, 450, 511
HpySE526I ACGT 2 cut(s) 70, 274
Hsp92II CATG 5 cut(s) 18, 485, 585, 672, 682
HspAI GCGC 3 cut(s) 215, 217, 283
Kpn2I TCCGGA 2 cut(s) 105, 687
Kzo9I GATC 6 cut(s) 65, 241, 314, 468, 493, 690
LmnI GCTCC 2 cut(s) 108, 690
LpnPI CCDG 5 cut(s) 119, 141, 438, 534, 701
LweI GCATC 1 cut(s) 498
MaeI CTAG 2 cut(s) 534, 653
MaeII ACGT 2 cut(s) 70, 274
MaeIII GTNAC 1 cut(s) 542
MalI GATC 6 cut(s) 67, 243, 316, 470, 495, 692
MboI GATC 6 cut(s) 65, 241, 314, 468, 493, 690
MboII GAAGA 2 cut(s) 424, 476
MflI RGATCY 2 cut(s) 468, 690
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 4 cut(s) 22, 318, 374, 617
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 142, 496
MnlI CCTC 6 cut(s) 138, 146, 289, 320, 376, 548
Mph1103I ATGCAT 1 cut(s) 452
MroI TCCGGA 2 cut(s) 105, 687
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 3 cut(s) 13, 237, 486
MspI CCGG 3 cut(s) 106, 521, 688
MvnI CGCG 3 cut(s) 64, 217, 246
NdeII GATC 6 cut(s) 65, 241, 314, 468, 493, 690
NlaIII CATG 5 cut(s) 18, 485, 585, 672, 682
NlaIV GGNNCC 2 cut(s) 203, 686
NmuCI GTSAC 1 cut(s) 542
NsbI TGCGCA 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 1 cut(s) 585
PauI GCGCGC 1 cut(s) 215
PciI ACATGT 1 cut(s) 581
PfeI GAWTC 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 520
PkrI GCNGC 1 cut(s) 684
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Ppu21I YACGTR 2 cut(s) 71, 275
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 411
PspN4I GGNNCC 2 cut(s) 203, 686
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 4 cut(s) 168, 201, 202, 411
PspPPI RGGWCCY 1 cut(s) 411
PsuI RGATCY 2 cut(s) 468, 690
PteI GCGCGC 1 cut(s) 215
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
RseI CAYNNNNRTG 3 cut(s) 13, 237, 486
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 1 cut(s) 683
Sau3AI GATC 6 cut(s) 65, 241, 314, 468, 493, 690
Sau96I GGNCC 4 cut(s) 168, 201, 202, 411
SchI GAGTC 1 cut(s) 524
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 1 cut(s) 498
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 3 cut(s) 13, 237, 486
Sse9I AATT 4 cut(s) 22, 318, 374, 617
SsiI CCGC 3 cut(s) 62, 246, 682
SspMI CTAG 2 cut(s) 534, 653
TaaI ACNGT 3 cut(s) 187, 540, 614
TaiI ACGT 2 cut(s) 73, 277
TaqI TCGA 1 cut(s) 381
TasI AATT 4 cut(s) 22, 318, 374, 617
TatI WGTACW 1 cut(s) 629
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 1 cut(s) 558
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 3 cut(s) 424, 498, 593
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 3 cut(s) 22, 374, 617
XbaI TCTAGA 1 cut(s) 533
XceI RCATGY 1 cut(s) 585
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmiI GTMKAC 2 cut(s) 73, 528
XspI CTAG 2 cut(s) 534, 653
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.