Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0002300

Physical Location & Seq

Genomic Coordinates Reverse (-)

19109832 .. 19118895

Gene Structure 9064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028134

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGTTTGTGAAGACGACGAGCCTGATGTCGAGATAGGCATACCTGCTGTTATGCTTCCCAACAAAATTGGTGAAATCTTGGAAAATGGATTAACGAACAATTCCATAGTGCTGTATATGTGGCTTCAATTCTTGCTGGGTGAAGGTGCCAGTCCTCTAGTGGAGATTAGCACCATGGCAGCAATTCTCTTTGTTGTGATTGCTTCATGCTTCTTGGTTATGTTTTACAAACTTATGTCCTTCTGGTTCATCGAGATTCTGGTGGTTTTGTTTTGCATTGGTGGGATAGAGGGCCTACAGACTTGCTTGGTGGCCTTGCTATCATGTTTCAGACGGTTTAAACGTGCTGGAGAATCATATGTTAAGGTGCCCTTCTTTGGAGCTGTTTTATATTTGACACTAGCTGTCGCTCCCTTCTGCATAGCATTTGCTGTTGTTTGGGCAGTGTATCGCCGTGTGTCATTTGCTTGGATTGGCCAAGATATCCTTGGTATTGCACTGATAATCACCGTTCTTCAGATTGTTCGCGTACCGAATCTCAAGGTTGGAACAGTTCTTCTCAGTTGTGCCTTTTTTTATGACATCTTCTGGGTATTTGTTTCTAAATGGTGGTTCCATGAGAGTGTCATGATAGTGGTGGCTCGTGGTGATAAAAGTGGAGAGGATGGTATCCCAATGCTACTAAAAATCCCACGGATGTTTGATCCTTGGGGTGGCTATAGCATCATTGGGTTTGGTGATATTATCTTACCTGGATTGGTTGTAGCCTTTTCACTAAGAGTTCTGTTTTTTGACGAGAAGGAACTGGGTATTGCACTGATAATCACCGTTCTTCAGATTGTTCGTGTACCAAATCTCAAGGTTGGAACAGTTCTTCTCGGTTGTGCCTTTTTTTATGACATCTTCTGGGTATTTGTTTCTAAATGGTGGTTCCATGTGAGTGTCATGATAGTGGTGGCTCGTGGTGATAAAAGTGGAGAGGATGGTATCCCAATGCTACTAAAAATCCCACGGATGTTTGATCCTTGGGGTGGCTACAGCATCATCGGGTTTGGTGATATTATCTTACCTGGATTGGTTGTAGCCTTTTCACTAAGGTATGATTGGTTGACAAATAAGACTCTTCGAACGGGTTACTTTGTGTGGGCAATGACAGCTTATGGTGCAGGTCTCCTGGTCACCTATGTGGCTTTGAACTTGATGGATGGACATGGCCAACCTGCTTTGTTATACGTAGTTCCATTCACACTTGGTACCTTCTTAACCTTGGGACAGATGAGAGGTGACCTCAAAGTTCTGTGGACAAGAGGAGAACCAGAGAGGCCTTGCCCGCACGTACGACTCCAATCCTCTCACTCTCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0012505 GO:0016020 GO:0016021 GO:0030176 GO:0030660 GO:0031090 GO:0031224 GO:0031227 GO:0031410 GO:0031982 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071458 GO:0071556 GO:0097708 GO:0098552 GO:0098553 GO:0098554 GO:0098562 GO:0098588 GO:0098805 GO:0098827 GO:0098852

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_A22B

Protein Analysis

455

Amino Acids

50.85

Weight (kDa)

6.24

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_A22B	PF04258	54 - 261	7.5e-69	Signal peptide peptidase
Peptidase_A22B	PF04258	269 - 438	5.6e-73	Signal peptide peptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01650 AT1G01650 AT1G01650 AT1G01650
fragaria_vesca	FvH4_7g14350 FvH4_7g14350 FvH4_7g14350 FvH4_7g14350 FvH4_7g14350
malus_domestica	MD01G1061500.v1.1 MD07G1136600.v1.1
prunus_persica	Prupe.2G171000_v2.0.a1 Prupe.2G171000_v2.0.a1
pyrus_communis	pycom01g09170 pycom07g13430
rosa_chinensis	RchiOBHm_Chr1g0355371
rosa_laevigata	RLG00000028134 RLG00000028139 RLG00000028145 RLG00000028149
rosa_multiflora	Rmu_sc0004484.1_g000010 Rmu_sc0021229.1_g000001
rosa_roxburghii	Rroxscaffold_4G00300080 Rroxscaffold_4G00300120 Rroxscaffold_4G00300280 Rroxscaffold_4G00300370 Rroxscaffold_4G00300460
rosa_rugosa	Rorug01G0246900
rosa_samantha	Rh1AG259400 Rh1AG259500 Rh1AG259800 Rh1AG260300 Rh1AG260900 Rh1BG229000 Rh1BG229200 Rh1CG243400 Rh1CG243500 Rh1DG256900
rosa_wichuraiana	Rw1G020180 Rw1G022870 Rw1G022900 Rw1G022920

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	52, 1157, 1228
Acc65I	GGTACC	1 cut(s)	1253
AccB1I	GGYRCC	3 cut(s)	146, 367, 1253
AccII	CGCG	1 cut(s)	528
AciI	CCGC	1 cut(s)	1331
AclWI	GGATC	2 cut(s)	698, 1016
AcoI	YGGCCR	2 cut(s)	475, 1213
AcuI	CTGAAG	2 cut(s)	500, 818
AfaI	GTAC	4 cut(s)	531, 849, 1255, 1338
AfiI	CCNNNNNNNGG	3 cut(s)	377, 713, 1031
AgsI	TTSAA	2 cut(s)	128, 1195
AjnI	CCWGG	3 cut(s)	751, 1069, 1173
AleI	CACNNNNGTG	1 cut(s)	1184
AluBI	AGCT	3 cut(s)	383, 404, 1157
AluI	AGCT	3 cut(s)	383, 404, 1157
Alw26I	GTCTC	1 cut(s)	1175
AlwI	GGATC	2 cut(s)	698, 1016
AoxI	GGCC	5 cut(s)	292, 312, 475, 1213, 1322
ApeKI	GCWGC	1 cut(s)	179
Asp718I	GGTACC	1 cut(s)	1253
AspS9I	GGNCC	1 cut(s)	292
AsuII	TTCGAA	1 cut(s)	1126
BaeGI	GKGCMC	1 cut(s)	372
BalI	TGGCCA	2 cut(s)	477, 1215
BanI	GGYRCC	3 cut(s)	146, 367, 1253
BauI	CACGAG	2 cut(s)	642, 960
BbsI	GAAGAC	1 cut(s)	18
BbvI	GCAGC	1 cut(s)	191
BccI	CCATC	4 cut(s)	659, 977, 1195, 1199
BceAI	ACGGC	1 cut(s)	438
BciT130I	CCWGG	3 cut(s)	753, 1071, 1175
BciVI	GTATCC	2 cut(s)	680, 998
BcoDI	GTCTC	1 cut(s)	1175
BfaI	CTAG	2 cut(s)	158, 401
BfmI	CTRYAG	3 cut(s)	296, 718, 1036
BfuAI	ACCTGC	3 cut(s)	52, 1157, 1228
BfuI	GTATCC	2 cut(s)	680, 998
BisI	GCNGC	1 cut(s)	180
BlsI	GCNGC	1 cut(s)	181
Bme1390I	CCNGG	3 cut(s)	753, 1071, 1175
BmgT120I	GGNCC	1 cut(s)	292
BmiI	GGNNCC	5 cut(s)	148, 369, 614, 932, 1255
BmrFI	CCNGG	3 cut(s)	753, 1071, 1175
BmrI	ACTGGG	1 cut(s)	815
BmsI	GCATC	2 cut(s)	732, 1050
BmuI	ACTGGG	1 cut(s)	815
BpiI	GAAGAC	1 cut(s)	18
BplI	GAGNNNNNCTC	2 cut(s)	1272, 1304
BpmI	CTGGAG	1 cut(s)	369
Bpu14I	TTCGAA	1 cut(s)	1126
BpuEI	CTTGAG	2 cut(s)	524, 842
BsaAI	YACGTR	2 cut(s)	1234, 1336
BsaI	GGTCTC	1 cut(s)	1175
BsaJI	CCNNGG	7 cut(s)	174, 487, 692, 707, 1010, 1025, 1266
Bsc4I	CCNNNNNNNGG	3 cut(s)	377, 713, 1031
Bse1I	ACTGG	2 cut(s)	150, 810
Bse3DI	GCAATG	1 cut(s)	1155
BseBI	CCWGG	3 cut(s)	753, 1071, 1175
BseDI	CCNNGG	7 cut(s)	174, 487, 692, 707, 1010, 1025, 1266
BseGI	GGATG	5 cut(s)	670, 702, 988, 1020, 1210
BseLI	CCNNNNNNNGG	3 cut(s)	377, 713, 1031
BseMI	GCAATG	1 cut(s)	1155
BseMII	CTCAG	1 cut(s)	574
BseNI	ACTGG	2 cut(s)	150, 810
BseRI	GAGGAG	1 cut(s)	1323
BseSI	GKGCMC	1 cut(s)	372
BseXI	GCAGC	1 cut(s)	191
BseYI	CCCAGC	1 cut(s)	136
BsgI	GTGCAG	1 cut(s)	1185
Bsh1236I	CGCG	1 cut(s)	528
BshFI	GGCC	5 cut(s)	294, 314, 477, 1215, 1324
BshNI	GGYRCC	3 cut(s)	146, 367, 1253
BsiWI	CGTACG	1 cut(s)	1336
BslFI	GGGAC	1 cut(s)	1284
BslI	CCNNNNNNNGG	3 cut(s)	377, 713, 1031
BsmAI	GTCTC	1 cut(s)	1175
BsmFI	GGGAC	1 cut(s)	1284
BsnI	GGCC	5 cut(s)	294, 314, 477, 1215, 1324
Bso31I	GGTCTC	1 cut(s)	1175
Bsp119I	TTCGAA	1 cut(s)	1126
Bsp1286I	GDGCHC	1 cut(s)	372
Bsp143I	GATC	2 cut(s)	703, 1021
Bsp19I	CCATGG	1 cut(s)	174
BspACI	CCGC	1 cut(s)	1331
BspANI	GGCC	5 cut(s)	294, 314, 477, 1215, 1324
BspCNI	CTCAG	1 cut(s)	573
BspFNI	CGCG	1 cut(s)	528
BspHI	TCATGA	2 cut(s)	627, 945
BspLI	GGNNCC	5 cut(s)	148, 369, 614, 932, 1255
BspMI	ACCTGC	3 cut(s)	52, 1157, 1228
BspPI	GGATC	2 cut(s)	698, 1016
BspT104I	TTCGAA	1 cut(s)	1126
BspT107I	GGYRCC	3 cut(s)	146, 367, 1253
BspTNI	GGTCTC	1 cut(s)	1175
BsrDI	GCAATG	1 cut(s)	1155
BsrI	ACTGG	2 cut(s)	150, 810
BssECI	CCNNGG	7 cut(s)	174, 487, 692, 707, 1010, 1025, 1266
BssMI	GATC	2 cut(s)	703, 1021
BssSI	CACGAG	2 cut(s)	642, 960
BssT1I	CCWWGG	5 cut(s)	174, 487, 707, 1025, 1266
Bst2BI	CACGAG	2 cut(s)	642, 960
Bst2UI	CCWGG	3 cut(s)	753, 1071, 1175
Bst4CI	ACNGT	5 cut(s)	336, 511, 553, 829, 871
Bst6I	CTCTTC	1 cut(s)	1128
BstBAI	YACGTR	2 cut(s)	1234, 1336
BstBI	TTCGAA	1 cut(s)	1126
BstC8I	GCNNGC	1 cut(s)	1331
BstDEI	CTNAG	3 cut(s)	560, 776, 1094
BstDSI	CCRYGG	3 cut(s)	174, 692, 1010
BstEII	GGTNACC	2 cut(s)	1177, 1283
BstF5I	GGATG	5 cut(s)	670, 702, 988, 1020, 1210
BstFNI	CGCG	1 cut(s)	528
BstKTI	GATC	2 cut(s)	706, 1024
BstMAI	GTCTC	1 cut(s)	1175
BstMBI	GATC	2 cut(s)	703, 1021
BstMWI	GCNNNNNNNGC	3 cut(s)	1154, 1163, 1330
BstNI	CCWGG	3 cut(s)	753, 1071, 1175
BstPI	GGTNACC	2 cut(s)	1177, 1283
BstSCI	CCNGG	3 cut(s)	751, 1069, 1173
BstSFI	CTRYAG	3 cut(s)	296, 718, 1036
BstSLI	GKGCMC	1 cut(s)	372
BstSNI	TACGTA	1 cut(s)	1234
BstUI	CGCG	1 cut(s)	528
BstV1I	GCAGC	1 cut(s)	191
BstV2I	GAAGAC	1 cut(s)	18
BsuI	GTATCC	2 cut(s)	680, 998
BsuRI	GGCC	5 cut(s)	294, 314, 477, 1215, 1324
BtgI	CCRYGG	3 cut(s)	174, 692, 1010
BtsCI	GGATG	5 cut(s)	670, 702, 988, 1020, 1210
BtsI	GCAGTG	1 cut(s)	450
BtsIMutI	CAGTG	3 cut(s)	450, 497, 815
BveI	ACCTGC	3 cut(s)	52, 1157, 1228
Cac8I	GCNNGC	1 cut(s)	1331
CciI	TCATGA	2 cut(s)	627, 945
Cfr13I	GGNCC	1 cut(s)	292
Csp6I	GTAC	4 cut(s)	530, 848, 1254, 1337
CviAII	CATG	8 cut(s)	175, 207, 324, 617, 628, 935, 946, 1211
CviQI	GTAC	4 cut(s)	530, 848, 1254, 1337
DdeI	CTNAG	3 cut(s)	560, 776, 1094
DpnI	GATC	2 cut(s)	705, 1023
DpnII	GATC	2 cut(s)	703, 1021
DraI	TTTAAA	1 cut(s)	340
EaeI	YGGCCR	2 cut(s)	475, 1213
Eam1104I	CTCTTC	1 cut(s)	1128
EarI	CTCTTC	1 cut(s)	1128
Eco105I	TACGTA	1 cut(s)	1234
Eco130I	CCWWGG	5 cut(s)	174, 487, 707, 1025, 1266
Eco147I	AGGCCT	1 cut(s)	1324
Eco31I	GGTCTC	1 cut(s)	1175
Eco32I	GATATC	1 cut(s)	484
Eco57I	CTGAAG	2 cut(s)	500, 818
Eco91I	GGTNACC	2 cut(s)	1177, 1283
EcoO109I	RGGNCCY	1 cut(s)	292
EcoO65I	GGTNACC	2 cut(s)	1177, 1283
EcoRII	CCWGG	3 cut(s)	751, 1069, 1173
EcoRV	GATATC	1 cut(s)	484
EcoT14I	CCWWGG	5 cut(s)	174, 487, 707, 1025, 1266
ErhI	CCWWGG	5 cut(s)	174, 487, 707, 1025, 1266
FaeI	CATG	8 cut(s)	178, 210, 327, 620, 631, 938, 949, 1214
FalI	AAGNNNNNCTT	4 cut(s)	356, 388, 471, 503
FaqI	GGGAC	1 cut(s)	1284
FatI	CATG	8 cut(s)	174, 206, 323, 616, 627, 934, 945, 1210
FauI	CCCGC	1 cut(s)	1338
FauNDI	CATATG	1 cut(s)	358
Fnu4HI	GCNGC	1 cut(s)	180
FokI	GGATG	5 cut(s)	677, 709, 995, 1027, 1217
Fsp4HI	GCNGC	1 cut(s)	180
FspBI	CTAG	2 cut(s)	158, 401
GluI	GCNGC	1 cut(s)	180
GsaI	CCCAGC	1 cut(s)	140
GsuI	CTGGAG	1 cut(s)	369
HaeIII	GGCC	5 cut(s)	294, 314, 477, 1215, 1324
Hin1II	CATG	8 cut(s)	178, 210, 327, 620, 631, 938, 949, 1214
HincII	GTYRAC	1 cut(s)	1110
HindII	GTYRAC	1 cut(s)	1110
HinfI	GANTC	5 cut(s)	256, 353, 535, 1120, 1341
Hpy166II	GTNNAC	3 cut(s)	848, 1110, 1302
Hpy188I	TCNGA	3 cut(s)	332, 519, 837
Hpy188III	TCNNGA	4 cut(s)	31, 253, 628, 946
Hpy8I	GTNNAC	3 cut(s)	848, 1110, 1302
Hpy99I	CGWCG	1 cut(s)	20
HpyAV	CCTTC	6 cut(s)	137, 250, 382, 424, 793, 1267
HpyCH4III	ACNGT	5 cut(s)	336, 511, 553, 829, 871
HpyCH4IV	ACGT	3 cut(s)	343, 1233, 1335
HpyCH4V	TGCA	5 cut(s)	276, 420, 497, 815, 1166
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1154, 1163, 1330
HpyF3I	CTNAG	3 cut(s)	560, 776, 1094
HpySE526I	ACGT	3 cut(s)	343, 1233, 1335
Hsp92II	CATG	8 cut(s)	178, 210, 327, 620, 631, 938, 949, 1214
KpnI	GGTACC	1 cut(s)	1257
Kzo9I	GATC	2 cut(s)	703, 1021
LmnI	GCTCC	2 cut(s)	380, 415
Lsp1109I	GCAGC	1 cut(s)	191
LweI	GCATC	2 cut(s)	732, 1050
MaeI	CTAG	2 cut(s)	158, 401
MaeII	ACGT	3 cut(s)	343, 1233, 1335
MaeIII	GTNAC	3 cut(s)	1133, 1177, 1283
MalI	GATC	2 cut(s)	705, 1023
MboI	GATC	2 cut(s)	703, 1021
MboII	GAAGA	8 cut(s)	23, 506, 548, 577, 824, 866, 895, 1115
MhlI	GDGCHC	1 cut(s)	372
MlsI	TGGCCA	2 cut(s)	477, 1215
MluCI	AATT	4 cut(s)	66, 100, 128, 183
MluNI	TGGCCA	2 cut(s)	477, 1215
MlyI	GAGTC	2 cut(s)	1114, 1335
MmeI	TCCRAC	2 cut(s)	526, 844
MnlI	CCTC	9 cut(s)	165, 283, 655, 973, 1274, 1298, 1301, 1314, 1360
Mox20I	TGGCCA	2 cut(s)	477, 1215
MscI	TGGCCA	2 cut(s)	477, 1215
MseI	TTAA	4 cut(s)	92, 339, 363, 1262
MslI	CAYNNNNRTG	5 cut(s)	621, 632, 939, 950, 1184
Msp20I	TGGCCA	2 cut(s)	477, 1215
MspR9I	CCNGG	3 cut(s)	753, 1071, 1175
MssI	GTTTAAAC	1 cut(s)	340
MvaI	CCWGG	3 cut(s)	753, 1071, 1175
MvnI	CGCG	1 cut(s)	528
MwoI	GCNNNNNNNGC	3 cut(s)	1154, 1163, 1330
NcoI	CCATGG	1 cut(s)	174
NdeI	CATATG	1 cut(s)	358
NdeII	GATC	2 cut(s)	703, 1021
NlaIII	CATG	8 cut(s)	178, 210, 327, 620, 631, 938, 949, 1214
NlaIV	GGNNCC	5 cut(s)	148, 369, 614, 932, 1255
NmuCI	GTSAC	2 cut(s)	1177, 1283
NspV	TTCGAA	1 cut(s)	1126
OliI	CACNNNNGTG	1 cut(s)	1184
PagI	TCATGA	2 cut(s)	627, 945
PceI	AGGCCT	1 cut(s)	1324
PcsI	WCGNNNNNNNCGW	1 cut(s)	340
PfeI	GAWTC	3 cut(s)	256, 353, 535
Pfl23II	CGTACG	1 cut(s)	1336
PkrI	GCNGC	1 cut(s)	181
PleI	GAGTC	2 cut(s)	1114, 1335
PmeI	GTTTAAAC	1 cut(s)	340
PpsI	GAGTC	2 cut(s)	1114, 1335
Ppu21I	YACGTR	2 cut(s)	1234, 1336
Psp6I	CCWGG	3 cut(s)	751, 1069, 1173
PspEI	GGTNACC	2 cut(s)	1177, 1283
PspFI	CCCAGC	1 cut(s)	136
PspGI	CCWGG	3 cut(s)	751, 1069, 1173
PspLI	CGTACG	1 cut(s)	1336
PspN4I	GGNNCC	5 cut(s)	148, 369, 614, 932, 1255
PspPI	GGNCC	1 cut(s)	292
RsaI	GTAC	4 cut(s)	531, 849, 1255, 1338
RsaNI	GTAC	4 cut(s)	530, 848, 1254, 1337
RseI	CAYNNNNRTG	5 cut(s)	621, 632, 939, 950, 1184
SaqAI	TTAA	4 cut(s)	92, 339, 363, 1262
SatI	GCNGC	1 cut(s)	180
Sau3AI	GATC	2 cut(s)	703, 1021
Sau96I	GGNCC	1 cut(s)	292
SchI	GAGTC	2 cut(s)	1114, 1335
ScrFI	CCNGG	3 cut(s)	753, 1071, 1175
SduI	GDGCHC	1 cut(s)	372
SfaNI	GCATC	2 cut(s)	732, 1050
SfcI	CTRYAG	3 cut(s)	296, 718, 1036
SfuI	TTCGAA	1 cut(s)	1126
SmiMI	CAYNNNNRTG	5 cut(s)	621, 632, 939, 950, 1184
SmlI	CTYRAG	2 cut(s)	539, 857
SmoI	CTYRAG	2 cut(s)	539, 857
SnaBI	TACGTA	1 cut(s)	1234
Sse9I	AATT	4 cut(s)	66, 100, 128, 183
SseBI	AGGCCT	1 cut(s)	1324
SsiI	CCGC	1 cut(s)	1331
SspMI	CTAG	2 cut(s)	158, 401
StuI	AGGCCT	1 cut(s)	1324
StyD4I	CCNGG	3 cut(s)	751, 1069, 1173
StyI	CCWWGG	5 cut(s)	174, 487, 707, 1025, 1266
TaaI	ACNGT	5 cut(s)	336, 511, 553, 829, 871
TaiI	ACGT	3 cut(s)	346, 1236, 1338
TaqI	TCGA	3 cut(s)	30, 252, 1126
TasI	AATT	4 cut(s)	66, 100, 128, 183
TfiI	GAWTC	3 cut(s)	256, 353, 535
Tru1I	TTAA	4 cut(s)	92, 339, 363, 1262
Tru9I	TTAA	4 cut(s)	92, 339, 363, 1262
TscAI	CASTG	3 cut(s)	450, 504, 822
TseFI	GTSAC	2 cut(s)	1177, 1283
TseI	GCWGC	1 cut(s)	179
Tsp45I	GTSAC	2 cut(s)	1177, 1283
TspDTI	ATGAA	2 cut(s)	195, 238
TspGWI	ACGGA	2 cut(s)	709, 1027
TspRI	CASTG	3 cut(s)	450, 504, 822
XcmI	CCANNNNNNNNNTGG	2 cut(s)	157, 485
XspI	CTAG	2 cut(s)	158, 401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.