Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0002300

Physical Location & Seq

Genomic Coordinates Forward (+)

50382768 .. 50390470

Gene Structure 7703 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG243400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGGCTGAATCTGCTAATGCTTCAGCCATCCTCATCATAAATAACCGTCCAGAACTTTTCAAGATGGTTTGTGAAGACGATGAGCCTGATGTCGAGATAGGCATACCTGCTGTTATGCTTCCCAACAAAATTGGTGAAATCTTGGAAAATGGATTAACGAACAATTCCATAGTTTCTGTGCAGCTATACTCTCCATTGCGTCCACTGGTTGACATTGCTGAAGTATTTTTATGGCTTATGGCTGTTGGTACCATCTTATTTGCTTCTTATTGGTCTGCATGGACTGCAAGAGAAGCAGCTATTGAGCATGACAAGCTATTGAAGGATGCTTCTGACGAGTCTTCACATGTGGAAGGTGAAAGTGCCAGTCCTCTAGTGGAGATTAGCACCATGGCAGCAATTCTCTTTGTTGTGATTGCTTCATGCTTCTTGGTTATGTTTTACAAACTTATGTCCTTCTGGTTCATCGAGATTCTGGTGGTTTTGTTTTGCATTGGTGGGATAGAGGGCCTACAGACTTGCTTGGTGGCCTTGCTATCATGTTTCAGACGGTTTAAACGTGCTGGAGAATCATATGTTAAGGTGCCCTTCTTTGGAGCTGTTTCATATTTGACACTAGCTGTCGCTCCCTTCTGCATAGCATTTGCTGTTGTTTGGGCAGTGTATCGCCGTGTGTCATTTGCTTGGATTGGCCAAGATATCCTTGGTATTGCACTGATAATCACCGTTCTTCAGATTGTTCGCGTACCGAATCTCAAGGTTGGAACAGTTCTTCTCGGTTGTGCCTTTTTTTATGACATCTTCTGGGTATTTGTTTCTAAATGGTGGTTCCATGAGAGTGTCATGATAGTGGTGGCTCGTGGTGATAAAAGTGGAGAGGATGGTATCCCAATGCTACTAAAAATCCCACGGATGTTTGATCCTTGGGGTGGCTACAGCATCATCGGGTTTGGTGATATTATCTTACCTGGATTGGTTGTAGCCTTTTCACTAAGGTATGATTGGTTGACAAATAAGACTCTTCGAACGGGTTACTTTGTGTGGGCAATGACAGCTTATGGTGCAGGTCTCCTGGTCACCTATGTGGCTTTGAACTTGATGGATGGACATGGCCAACCTGCTTTGTTATACATAGTTCCATTCACACTTGGTACCTTCTTAACCTTGGGACAGATGAGAGGTGACCTCAAAGTTCTGTGGACAAGAGGAGAACCTGAGAGGCCTTGCCCGCACGTACGACTCCAATCCTCTCACTCTCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0012505 GO:0016020 GO:0016021 GO:0030176 GO:0030660 GO:0031090 GO:0031224 GO:0031227 GO:0031410 GO:0031982 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071458 GO:0071556 GO:0097708 GO:0098552 GO:0098553 GO:0098554 GO:0098562 GO:0098588 GO:0098805 GO:0098827 GO:0098852

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_A22B

Protein Analysis

420

Amino Acids

46.77

Weight (kDa)

5.47

Isoelectric Point (pI)

39.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_A22B	PF04258	125 - 404	6.3e-103	Signal peptide peptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01650 AT1G01650 AT1G01650 AT1G01650
fragaria_vesca	FvH4_7g14350 FvH4_7g14350 FvH4_7g14350 FvH4_7g14350 FvH4_7g14350
malus_domestica	MD01G1061500.v1.1 MD07G1136600.v1.1
prunus_persica	Prupe.2G171000_v2.0.a1 Prupe.2G171000_v2.0.a1
pyrus_communis	pycom01g09170 pycom07g13430
rosa_chinensis	RchiOBHm_Chr1g0355371
rosa_laevigata	RLG00000028134 RLG00000028139 RLG00000028145 RLG00000028149
rosa_multiflora	Rmu_sc0004484.1_g000010 Rmu_sc0021229.1_g000001
rosa_roxburghii	Rroxscaffold_4G00300080 Rroxscaffold_4G00300120 Rroxscaffold_4G00300280 Rroxscaffold_4G00300370 Rroxscaffold_4G00300460
rosa_rugosa	Rorug01G0246900
rosa_samantha	Rh1AG259400 Rh1AG259500 Rh1AG259800 Rh1AG260300 Rh1AG260900 Rh1BG229000 Rh1BG229200 Rh1CG243400 Rh1CG243500 Rh1DG256900
rosa_wichuraiana	Rw1G020180 Rw1G022870 Rw1G022900 Rw1G022920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	115, 1055, 1126
Acc65I	GGTACC	2 cut(s)	248, 1151
AccB1I	GGYRCC	3 cut(s)	248, 583, 1151
AccII	CGCG	1 cut(s)	744
AciI	CCGC	1 cut(s)	1229
AclWI	GGATC	1 cut(s)	914
AcoI	YGGCCR	2 cut(s)	691, 1111
AcuI	CTGAAG	3 cut(s)	6, 240, 716
AfaI	GTAC	4 cut(s)	250, 747, 1153, 1236
AfiI	CCNNNNNNNGG	2 cut(s)	593, 929
AflIII	ACRYGT	1 cut(s)	346
AgsI	TTSAA	3 cut(s)	61, 322, 1093
AjnI	CCWGG	2 cut(s)	967, 1071
AleI	CACNNNNGTG	1 cut(s)	1082
AluBI	AGCT	6 cut(s)	184, 299, 316, 599, 620, 1055
AluI	AGCT	6 cut(s)	184, 299, 316, 599, 620, 1055
Alw26I	GTCTC	1 cut(s)	1073
AlwI	GGATC	1 cut(s)	914
AoxI	GGCC	5 cut(s)	508, 528, 691, 1111, 1220
ApeKI	GCWGC	3 cut(s)	181, 296, 395
Asp718I	GGTACC	2 cut(s)	248, 1151
AspS9I	GGNCC	1 cut(s)	508
AsuHPI	GGTGA	7 cut(s)	146, 368, 715, 875, 965, 1069, 1193
AsuII	TTCGAA	1 cut(s)	1024
BaeGI	GKGCMC	1 cut(s)	588
BalI	TGGCCA	2 cut(s)	693, 1113
BanI	GGYRCC	3 cut(s)	248, 583, 1151
BauI	CACGAG	1 cut(s)	858
BbsI	GAAGAC	2 cut(s)	81, 333
BbvI	GCAGC	3 cut(s)	193, 308, 407
BccI	CCATC	6 cut(s)	35, 58, 260, 875, 1093, 1097
BceAI	ACGGC	1 cut(s)	654
BciT130I	CCWGG	2 cut(s)	969, 1073
BciVI	GTATCC	1 cut(s)	896
BcoDI	GTCTC	1 cut(s)	1073
BfaI	CTAG	2 cut(s)	374, 617
BfmI	CTRYAG	2 cut(s)	512, 934
BfuAI	ACCTGC	3 cut(s)	115, 1055, 1126
BfuI	GTATCC	1 cut(s)	896
BisI	GCNGC	3 cut(s)	182, 297, 396
BlsI	GCNGC	3 cut(s)	183, 298, 397
Bme1390I	CCNGG	2 cut(s)	969, 1073
BmgT120I	GGNCC	1 cut(s)	508
BmiI	GGNNCC	4 cut(s)	250, 585, 830, 1153
BmrFI	CCNGG	2 cut(s)	969, 1073
BmsI	GCATC	2 cut(s)	316, 948
BpiI	GAAGAC	2 cut(s)	81, 333
BplI	GAGNNNNNCTC	2 cut(s)	1170, 1202
BpmI	CTGGAG	1 cut(s)	585
Bpu14I	TTCGAA	1 cut(s)	1024
BpuEI	CTTGAG	1 cut(s)	740
BsaAI	YACGTR	1 cut(s)	1234
BsaI	GGTCTC	1 cut(s)	1073
BsaJI	CCNNGG	5 cut(s)	390, 703, 908, 923, 1164
Bsc4I	CCNNNNNNNGG	2 cut(s)	593, 929
Bse1I	ACTGG	2 cut(s)	210, 366
Bse3DI	GCAATG	3 cut(s)	194, 213, 1053
BseBI	CCWGG	2 cut(s)	969, 1073
BseDI	CCNNGG	5 cut(s)	390, 703, 908, 923, 1164
BseGI	GGATG	5 cut(s)	27, 331, 886, 918, 1108
BseLI	CCNNNNNNNGG	2 cut(s)	593, 929
BseMI	GCAATG	3 cut(s)	194, 213, 1053
BseMII	CTCAG	1 cut(s)	1206
BseNI	ACTGG	2 cut(s)	210, 366
BseRI	GAGGAG	1 cut(s)	1221
BseSI	GKGCMC	1 cut(s)	588
BseXI	GCAGC	3 cut(s)	193, 308, 407
BsgI	GTGCAG	2 cut(s)	200, 1083
Bsh1236I	CGCG	1 cut(s)	744
BshFI	GGCC	5 cut(s)	510, 530, 693, 1113, 1222
BshNI	GGYRCC	3 cut(s)	248, 583, 1151
BsiWI	CGTACG	1 cut(s)	1234
BslFI	GGGAC	1 cut(s)	1182
BslI	CCNNNNNNNGG	2 cut(s)	593, 929
BsmAI	GTCTC	1 cut(s)	1073
BsmFI	GGGAC	1 cut(s)	1182
BsnI	GGCC	5 cut(s)	510, 530, 693, 1113, 1222
Bso31I	GGTCTC	1 cut(s)	1073
Bsp119I	TTCGAA	1 cut(s)	1024
Bsp1286I	GDGCHC	1 cut(s)	588
Bsp143I	GATC	1 cut(s)	919
Bsp19I	CCATGG	1 cut(s)	390
BspACI	CCGC	1 cut(s)	1229
BspANI	GGCC	5 cut(s)	510, 530, 693, 1113, 1222
BspCNI	CTCAG	1 cut(s)	1207
BspFNI	CGCG	1 cut(s)	744
BspHI	TCATGA	1 cut(s)	843
BspLI	GGNNCC	4 cut(s)	250, 585, 830, 1153
BspMI	ACCTGC	3 cut(s)	115, 1055, 1126
BspPI	GGATC	1 cut(s)	914
BspT104I	TTCGAA	1 cut(s)	1024
BspT107I	GGYRCC	3 cut(s)	248, 583, 1151
BspTNI	GGTCTC	1 cut(s)	1073
BsrDI	GCAATG	3 cut(s)	194, 213, 1053
BsrI	ACTGG	2 cut(s)	210, 366
BssECI	CCNNGG	5 cut(s)	390, 703, 908, 923, 1164
BssMI	GATC	1 cut(s)	919
BssSI	CACGAG	1 cut(s)	858
BssT1I	CCWWGG	4 cut(s)	390, 703, 923, 1164
Bst2BI	CACGAG	1 cut(s)	858
Bst2UI	CCWGG	2 cut(s)	969, 1073
Bst4CI	ACNGT	4 cut(s)	47, 552, 727, 769
Bst6I	CTCTTC	1 cut(s)	1026
BstAPI	GCANNNNNTGC	1 cut(s)	284
BstBAI	YACGTR	1 cut(s)	1234
BstBI	TTCGAA	1 cut(s)	1024
BstC8I	GCNNGC	1 cut(s)	1229
BstDEI	CTNAG	2 cut(s)	992, 1215
BstDSI	CCRYGG	2 cut(s)	390, 908
BstEII	GGTNACC	2 cut(s)	1075, 1181
BstF5I	GGATG	5 cut(s)	27, 331, 886, 918, 1108
BstFNI	CGCG	1 cut(s)	744
BstKTI	GATC	1 cut(s)	922
BstMAI	GTCTC	1 cut(s)	1073
BstMBI	GATC	1 cut(s)	919
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 284, 293, 313, 1052, 1061, 1228
BstNI	CCWGG	2 cut(s)	969, 1073
BstNSI	RCATGY	1 cut(s)	350
BstPI	GGTNACC	2 cut(s)	1075, 1181
BstSCI	CCNGG	2 cut(s)	967, 1071
BstSFI	CTRYAG	2 cut(s)	512, 934
BstSLI	GKGCMC	1 cut(s)	588
BstUI	CGCG	1 cut(s)	744
BstV1I	GCAGC	3 cut(s)	193, 308, 407
BstV2I	GAAGAC	2 cut(s)	81, 333
BsuI	GTATCC	1 cut(s)	896
BsuRI	GGCC	5 cut(s)	510, 530, 693, 1113, 1222
BtgI	CCRYGG	2 cut(s)	390, 908
BtsCI	GGATG	5 cut(s)	27, 331, 886, 918, 1108
BtsI	GCAGTG	1 cut(s)	666
BtsIMutI	CAGTG	3 cut(s)	203, 666, 713
BveI	ACCTGC	3 cut(s)	115, 1055, 1126
Cac8I	GCNNGC	1 cut(s)	1229
CciI	TCATGA	1 cut(s)	843
Cfr13I	GGNCC	1 cut(s)	508
CseI	GACGC	1 cut(s)	188
Csp6I	GTAC	4 cut(s)	249, 746, 1152, 1235
CviAII	CATG	9 cut(s)	279, 308, 347, 391, 423, 540, 833, 844, 1109
CviQI	GTAC	4 cut(s)	249, 746, 1152, 1235
DdeI	CTNAG	2 cut(s)	992, 1215
DpnI	GATC	1 cut(s)	921
DpnII	GATC	1 cut(s)	919
DraI	TTTAAA	1 cut(s)	556
EaeI	YGGCCR	2 cut(s)	691, 1111
Eam1104I	CTCTTC	1 cut(s)	1026
EarI	CTCTTC	1 cut(s)	1026
Eco130I	CCWWGG	4 cut(s)	390, 703, 923, 1164
Eco147I	AGGCCT	1 cut(s)	1222
Eco31I	GGTCTC	1 cut(s)	1073
Eco32I	GATATC	1 cut(s)	700
Eco57I	CTGAAG	3 cut(s)	6, 240, 716
Eco91I	GGTNACC	2 cut(s)	1075, 1181
EcoO109I	RGGNCCY	1 cut(s)	508
EcoO65I	GGTNACC	2 cut(s)	1075, 1181
EcoRII	CCWGG	2 cut(s)	967, 1071
EcoRV	GATATC	1 cut(s)	700
EcoT14I	CCWWGG	4 cut(s)	390, 703, 923, 1164
ErhI	CCWWGG	4 cut(s)	390, 703, 923, 1164
FaeI	CATG	9 cut(s)	282, 311, 350, 394, 426, 543, 836, 847, 1112
FalI	AAGNNNNNCTT	4 cut(s)	572, 604, 687, 719
FaqI	GGGAC	1 cut(s)	1182
FatI	CATG	9 cut(s)	278, 307, 346, 390, 422, 539, 832, 843, 1108
FauI	CCCGC	1 cut(s)	1236
FauNDI	CATATG	1 cut(s)	574
Fnu4HI	GCNGC	3 cut(s)	182, 297, 396
FokI	GGATG	5 cut(s)	14, 338, 893, 925, 1115
Fsp4HI	GCNGC	3 cut(s)	182, 297, 396
FspBI	CTAG	2 cut(s)	374, 617
GluI	GCNGC	3 cut(s)	182, 297, 396
GsuI	CTGGAG	1 cut(s)	585
HaeIII	GGCC	5 cut(s)	510, 530, 693, 1113, 1222
HgaI	GACGC	1 cut(s)	188
Hin1II	CATG	9 cut(s)	282, 311, 350, 394, 426, 543, 836, 847, 1112
HincII	GTYRAC	2 cut(s)	211, 1008
HindII	GTYRAC	2 cut(s)	211, 1008
HinfI	GANTC	7 cut(s)	8, 338, 472, 569, 751, 1018, 1239
HphI	GGTGA	7 cut(s)	146, 368, 715, 875, 965, 1069, 1193
Hpy166II	GTNNAC	4 cut(s)	203, 211, 1008, 1200
Hpy188I	TCNGA	3 cut(s)	334, 548, 735
Hpy188III	TCNNGA	5 cut(s)	50, 61, 94, 469, 844
Hpy8I	GTNNAC	4 cut(s)	203, 211, 1008, 1200
HpyAV	CCTTC	6 cut(s)	316, 347, 466, 598, 640, 1165
HpyCH4III	ACNGT	4 cut(s)	47, 552, 727, 769
HpyCH4IV	ACGT	2 cut(s)	559, 1233
HpyCH4V	TGCA	7 cut(s)	181, 278, 287, 492, 636, 713, 1064
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 284, 293, 313, 1052, 1061, 1228
HpyF3I	CTNAG	2 cut(s)	992, 1215
HpySE526I	ACGT	2 cut(s)	559, 1233
Hsp92II	CATG	9 cut(s)	282, 311, 350, 394, 426, 543, 836, 847, 1112
KpnI	GGTACC	2 cut(s)	252, 1155
Kzo9I	GATC	1 cut(s)	919
LmnI	GCTCC	2 cut(s)	596, 631
Lsp1109I	GCAGC	3 cut(s)	193, 308, 407
LweI	GCATC	2 cut(s)	316, 948
MaeI	CTAG	2 cut(s)	374, 617
MaeII	ACGT	2 cut(s)	559, 1233
MaeIII	GTNAC	3 cut(s)	1031, 1075, 1181
MalI	GATC	1 cut(s)	921
MboI	GATC	1 cut(s)	919
MboII	GAAGA	6 cut(s)	86, 333, 722, 764, 793, 1013
MhlI	GDGCHC	1 cut(s)	588
MlsI	TGGCCA	2 cut(s)	693, 1113
MluCI	AATT	3 cut(s)	129, 163, 399
MluNI	TGGCCA	2 cut(s)	693, 1113
MlyI	GAGTC	3 cut(s)	347, 1012, 1233
MmeI	TCCRAC	1 cut(s)	742
MnlI	CCTC	9 cut(s)	41, 381, 499, 871, 1172, 1196, 1199, 1212, 1258
Mox20I	TGGCCA	2 cut(s)	693, 1113
MscI	TGGCCA	2 cut(s)	693, 1113
MseI	TTAA	4 cut(s)	155, 555, 579, 1160
MslI	CAYNNNNRTG	3 cut(s)	837, 848, 1082
Msp20I	TGGCCA	2 cut(s)	693, 1113
MspR9I	CCNGG	2 cut(s)	969, 1073
MssI	GTTTAAAC	1 cut(s)	556
MvaI	CCWGG	2 cut(s)	969, 1073
MvnI	CGCG	1 cut(s)	744
MwoI	GCNNNNNNNGC	7 cut(s)	11, 284, 293, 313, 1052, 1061, 1228
NcoI	CCATGG	1 cut(s)	390
NdeI	CATATG	1 cut(s)	574
NdeII	GATC	1 cut(s)	919
NlaIII	CATG	9 cut(s)	282, 311, 350, 394, 426, 543, 836, 847, 1112
NlaIV	GGNNCC	4 cut(s)	250, 585, 830, 1153
NmuCI	GTSAC	2 cut(s)	1075, 1181
NspI	RCATGY	1 cut(s)	350
NspV	TTCGAA	1 cut(s)	1024
OliI	CACNNNNGTG	1 cut(s)	1082
PagI	TCATGA	1 cut(s)	843
PceI	AGGCCT	1 cut(s)	1222
PciI	ACATGT	1 cut(s)	346
PcsI	WCGNNNNNNNCGW	1 cut(s)	556
PfeI	GAWTC	4 cut(s)	8, 472, 569, 751
Pfl23II	CGTACG	1 cut(s)	1234
PkrI	GCNGC	3 cut(s)	183, 298, 397
PleI	GAGTC	3 cut(s)	346, 1012, 1233
PmeI	GTTTAAAC	1 cut(s)	556
PpsI	GAGTC	3 cut(s)	346, 1012, 1233
Ppu21I	YACGTR	1 cut(s)	1234
PscI	ACATGT	1 cut(s)	346
Psp6I	CCWGG	2 cut(s)	967, 1071
PspEI	GGTNACC	2 cut(s)	1075, 1181
PspGI	CCWGG	2 cut(s)	967, 1071
PspLI	CGTACG	1 cut(s)	1234
PspN4I	GGNNCC	4 cut(s)	250, 585, 830, 1153
PspPI	GGNCC	1 cut(s)	508
RsaI	GTAC	4 cut(s)	250, 747, 1153, 1236
RsaNI	GTAC	4 cut(s)	249, 746, 1152, 1235
RseI	CAYNNNNRTG	3 cut(s)	837, 848, 1082
SaqAI	TTAA	4 cut(s)	155, 555, 579, 1160
SatI	GCNGC	3 cut(s)	182, 297, 396
Sau3AI	GATC	1 cut(s)	919
Sau96I	GGNCC	1 cut(s)	508
SchI	GAGTC	3 cut(s)	347, 1012, 1233
ScrFI	CCNGG	2 cut(s)	969, 1073
SduI	GDGCHC	1 cut(s)	588
SfaNI	GCATC	2 cut(s)	316, 948
SfcI	CTRYAG	2 cut(s)	512, 934
SfuI	TTCGAA	1 cut(s)	1024
SmiMI	CAYNNNNRTG	3 cut(s)	837, 848, 1082
SmlI	CTYRAG	1 cut(s)	755
SmoI	CTYRAG	1 cut(s)	755
Sse9I	AATT	3 cut(s)	129, 163, 399
SseBI	AGGCCT	1 cut(s)	1222
SsiI	CCGC	1 cut(s)	1229
SspMI	CTAG	2 cut(s)	374, 617
StuI	AGGCCT	1 cut(s)	1222
StyD4I	CCNGG	2 cut(s)	967, 1071
StyI	CCWWGG	4 cut(s)	390, 703, 923, 1164
TaaI	ACNGT	4 cut(s)	47, 552, 727, 769
TaiI	ACGT	2 cut(s)	562, 1236
TaqI	TCGA	3 cut(s)	93, 468, 1024
TasI	AATT	3 cut(s)	129, 163, 399
TfiI	GAWTC	4 cut(s)	8, 472, 569, 751
Tru1I	TTAA	4 cut(s)	155, 555, 579, 1160
Tru9I	TTAA	4 cut(s)	155, 555, 579, 1160
TscAI	CASTG	3 cut(s)	210, 666, 720
TseFI	GTSAC	2 cut(s)	1075, 1181
TseI	GCWGC	3 cut(s)	181, 296, 395
Tsp45I	GTSAC	2 cut(s)	1075, 1181
TspDTI	ATGAA	3 cut(s)	411, 454, 594
TspGWI	ACGGA	1 cut(s)	925
TspRI	CASTG	3 cut(s)	210, 666, 720
XceI	RCATGY	1 cut(s)	350
XcmI	CCANNNNNNNNNTGG	2 cut(s)	373, 701
XspI	CTAG	2 cut(s)	374, 617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.