Rmu_sc0000012.1_g000009

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000012.1
OG0002321
Physical Location & Seq
Forward (+)
38314 .. 51654
13341 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4308 bp
atgaacaggaacacgaagcgaggcggcggccgcggtgggggaggctcagctcccaagggcggaagaggaggagacagaaagggagccaaaaaaggcaagaggcagtcgttcaaggaagacccatttttcaccaacgaatccaagaagcggctcaaatacgacgaggacattcggagcgacgagtcgtccgacgatgaacgaaacgacgccgtcggtagtggcgaggaggaggaggaggaggaggcggctgaggagattatagaggagactcccggggagaagaagctgaggctgacgaaggagtatctgaagaggcttggagagcatgagaggaagaagaaggaagacgaggatgacgatagcgatgacgacagcgagaaggaaggcgacagagactcgcttgtcgccaaggttttgcagcagcagcagctccaggacagcggccgcctccgccgctccattgcttctagagttgtgagctttaggacttccggttttgatttgaattttgatcaggtggttttgtgtcagaactggatctatagtggcattttaattgttattgagatcctgttgtgttttgatgaagtgcattttgatcagatactagcgtcaggtttttggcttatgtatcttgttgtcgacattttacctgaaagaattagtgtttacattgtgcgttctgaagatctttgctttctgcacctcatttttgtggttcagaagccagaagctactgatgagtttcggatcttagtgaagcatcgccaatctgtcactgcagtggttctctctgatgatgatggcagaggtttttcagcatctaaggacggcgcgattttgcattgggatgtagatagtgggaagaaagagaagtatcagtggcccagtgacgaagtattgaagtcccacggggtcaaggaaccactaggtagagctacaaagcatagcaaacaaatgttagcacttgcagttagttctgatggtcggtatttggcaagtggaggattagatcgccatattcatttgtgggatactcgtaccagacagcatgttcaggcttttccgggtcacaggggacctgtatcatgtttaactttcaggcaagggagttcagaactcttttctggttcatatgatcgaacagttaagatatggaatgcagaagacagaacttacataaatacattatatggtcaccaaggtgaagtattatctatggattgcctacggaaagaacggcttttgactgttggacatgatcgaagcatgatgttattcaaggttccagaggagtcgcgtttagtattccgtgcccctgcatgctcattggagtgttgttcttttattagcaatgatgaattcttatccggctctgataatggaagtgttgagctttggaccatgctgcgaaagaagcctgtatgcatagttaagaatgctcatcctttgttagctgcacacaaagatgttgaacagaaggatggtgatagagtccccaatggtcacatagaaagctatgattgttcatcaacatattcttggatcagttcagtcacttcttgtcgaggcagtgaccttgccgcatcaggagctggtaatggctttgttcgtttatggtctattgaaagtgagagcaatgccattcagcccttacacagtctccccgtggttggatttgttaattctttagctattgcaaaatctggaggctttctagttgcaggagtcggaaaggttggatttgttaattctttagctattgcaaaatctggaggctttctagttgcaggagtcggaaaggaaccgcgtctaggaaggtggggacttatcccagctgctcagaatggagttgcaatccacacccttacgctttccagcggtcatcatgagcttaagccctctcaatttaagcctcgcgttctatctggtttgttcatcgggattcgcggatccccggttcggttcggcacaattccaaccatgttttggcgtatggccggcttgtccacggcgtctccggtggagacgattttggacaaggataatttcacattggaagagcttcttgatgaagatgagattattcaagaatgcaaagctcttaatgggcgcctaatcaatttcttgcgagaaagagtccaggtggagcaactcattcgatatattgttgaagaagctcccgaggatgctgaaaagaggcgaactttcaaatttccttttattgcgtgtgagatttttacttgtgaagttgatatcatactcaaaacattggtagaggatgaggagttgatgaatttgttgttctcctttctggaaccaaatcattcccatagcacactcttggctggctacttcagtaaggttgttgtttgcctgctgttgcgtaaggcagttccttttctgcactatattcagggtcatcaggatattgtcaagaagctagttgacctcattggaataacatcaatcatggaggttttaatacgtctggttggtgctgatgaacatatgtattcaaactatatggaagcaatgcactggatacaagatactgatgtgctagagatgattgtggacaagttcagctcttcagattctcctgaagtgcatgctaatgcagcagagacactttgtgctataactaggtttgctccacctggactttcttctaaaatctccagcccaagtttcataggaagattgtttcggcatgctttggaggactctcggccaaaatcggttcttgttaactctttgtcagtatgtatctctttgttagaccctaggaggctaactttgggaacatatcatatgtatggacgtcaaatgactcatggatccgcagttactgccaaccctgagactgttgtcggcatgttggagagcctaggtgatttgctgaaactcttggatgtttcatcagtagacaatacgttgttaactacatatggcaagttacagccacctcttggaaaacatcgcttaaagattgtagagttcatttcggtattacttacagtcggtagtgaagctgccgagaaggaattggttagtcttggagcagtgcaaagaattttagacttgtttttcaagtacccgtataacaactttgtgcatcaccacatagagaatatattagtgtcatgtttggaaagcaagaatgctcctcttgttgagcatcttcttcgcgaatgtgatctggttggaaaaatacttcaagcagaaaagaattctacattgtcagctgactcggacaagccaactattcctgctgagggtagacccgcacccaggatagggaatattggacatttaacacgtatatccaataaactcattcagctggggaataacaacagcgagattgaggcatatctgcaggagaacagcgagtggactgattggcagacaaatgttctatcgaaacgtaatgcactggagaatgtctatcagtgggcatgtgggcgaccaacagctttgcatgatcgaaatagggatagtgatgatgatgattaccaagatagggattatgacgttgcagctctcgctaataacttgagccaggcttttcgatatggcctttacagcaatgatgaattagatgagggccagggttcacttgaacgagatgatgaggatgtatactttgatgatgaatctgcagaagttgtcatatcttcactgcgtcttggagatgaccaggaaagtggttctctgtttacaaattccaactggttcgcatttgaggacgatagggcttccagtgatcgtgcaactggatcccttacctctccatctcctgataccatggaggctaatgttaccaatggtggtggtgaggataatgtcattgttggtgaagatgatgacttggttgacactgcaacatcttctccagaaccgggaacaaaacctgaagattcagaagttagcgtgcccaacaattctacagaagtgggatccagtgaaactgataagccaccggagtgggttgaatggagggaaacttcagattctggggaaccatctgatgccctacctaatggtgaacttcaaacggtatctccagatgcagcgacgagttccccatcctctgatccattggtaagtaaagatgaaattgatgctggacatcaagcagaaatatcagctgagaaaacttcaaaaccttctgagcttttagaatctggaaatgggaatccaagttctgattcaaccacttctgatgctgcagcaagtgctgttggaacaaagggcactcaagaagcagcagccactgtaggggatgaaaaggaagggaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

1435

Amino Acids

158.57

Weight (kDa)

4.97

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 246 - 478 3.8e-13 WDR55
WD40_CDC20-Fz PF24807 246 - 430 3.7e-16 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 252 - 394 4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 258 - 420 1.9e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 276 - 425 4.6e-06 WDR36/Utp21 first beta-propeller
NBCH_WD40 PF20426 287 - 410 7.4e-06 Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd PF25047 295 - 425 6.6e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 308 - 368 6e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 311 - 428 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 313 - 421 3.1e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 313 - 345 8.9e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 314 - 474 2.3e-22 WDR5 beta-propeller domain
Beta-prop_EML PF23409 314 - 482 2.8e-10 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 315 - 432 2.4e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 315 - 429 9.9e-23 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 316 - 469 6.3e-10 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 318 - 476 2e-18 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 319 - 432 1.4e-14 WDR3 second beta-propeller domain
WD40 PF00400 351 - 387 8.2e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 358 - 434 8.2e-08 THOC3 beta-propeller domain
SAPS PF04499 792 - 1150 1e-63 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
AatII GACGTC 1 cut(s) 2827
AccB1I GGYRCC 1 cut(s) 2112
AccB7I CCANNNNNTGG 2 cut(s) 1989, 2972
AccBSI CCGCTC 1 cut(s) 456
AccI GTMKAC 4 cut(s) 642, 2928, 3283, 3645
AccII CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
AcoI YGGCCR 4 cut(s) 28, 442, 1998, 2732
AcsI RAATTY 7 cut(s) 505, 1361, 2213, 2296, 3075, 3232, 3727
AcuI CTGAAG 7 cut(s) 329, 705, 2341, 2577, 2625, 3939, 3996
AcyI GRCGYC 4 cut(s) 207, 2015, 2113, 2824
AdeI CACNNNGTG 1 cut(s) 2636
AfaI GTAC 2 cut(s) 1042, 3098
AflII CTTAAG 1 cut(s) 1895
AflIII ACRYGT 1 cut(s) 3320
AhdI GACNNNNNGTC 1 cut(s) 184
AjnI CCWGG 7 cut(s) 432, 2142, 2659, 3293, 3564, 3612, 3702
AjuI GAANNNNNNNTTGG 2 cut(s) 4198, 4230
AleI CACNNNNGTG 3 cut(s) 782, 1203, 3988
AloI GAACNNNNNNTCC 4 cut(s) 1944, 1976, 4192, 4224
Alw26I GTCTC 8 cut(s) 66, 260, 387, 1667, 2021, 2022, 2621, 2858
AlwNI CAGNNNCTG 4 cut(s) 1595, 2852, 4019, 4280
Ama87I CYCGRG 2 cut(s) 272, 2183
AoxI GGCC 7 cut(s) 28, 442, 884, 1998, 2732, 3580, 3610
ApoI RAATTY 7 cut(s) 505, 1361, 2213, 2296, 3075, 3232, 3727
Asp700I GAANNNNTTC 1 cut(s) 2064
AspA2I CCTAGG 2 cut(s) 2786, 2890
AspLEI GCGC 2 cut(s) 836, 2115
AspS9I GGNCC 4 cut(s) 885, 1079, 1401, 3610
AsuC2I CCSGG 5 cut(s) 273, 274, 1068, 1958, 3906
AsuHPI GGTGA 9 cut(s) 121, 1190, 1217, 1499, 2906, 3113, 3851, 3872, 4061
AvaI CYCGRG 2 cut(s) 272, 2183
AvaII GGWCC 2 cut(s) 1079, 1401
AvrII CCTAGG 2 cut(s) 2786, 2890
BaeGI GKGCMC 3 cut(s) 1318, 3942, 4262
BaeI ACNNNNGTAYC 4 cut(s) 596, 629, 2544, 2577
BamHI GGATCC 4 cut(s) 1952, 2840, 3782, 3962
BanI GGYRCC 1 cut(s) 2112
BbsI GAAGAC 3 cut(s) 123, 351, 1173
BbvCI CCTCAGC 3 cut(s) 249, 287, 3276
BccI CCATC 6 cut(s) 797, 977, 1478, 3805, 4036, 4099
BceAI ACGGC 4 cut(s) 194, 847, 1256, 2028
BcgI CGANNNNNNTGC 4 cut(s) 2141, 2175, 3170, 3204
BciT130I CCWGG 7 cut(s) 434, 2144, 2661, 3295, 3566, 3614, 3704
BciVI GTATCC 2 cut(s) 1027, 2538
BclI TGATCA 2 cut(s) 511, 598
BcnI CCSGG 5 cut(s) 273, 274, 1068, 1958, 3906
BcoDI GTCTC 8 cut(s) 66, 260, 387, 1667, 2021, 2022, 2621, 2858
BfmI CTRYAG 7 cut(s) 541, 780, 3380, 3663, 3951, 4233, 4281
BfoI RGCGCY 1 cut(s) 2116
BfrI CTTAAG 1 cut(s) 1895
BfuI GTATCC 2 cut(s) 1027, 2538
BglII AGATCT 1 cut(s) 688
BlnI CCTAGG 2 cut(s) 2786, 2890
BlpI GCTNAGC 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 1079, 1401
BmeRI GACNNNNNGTC 1 cut(s) 184
BmeT110I CYCGRG 2 cut(s) 272, 2183
BmgT120I GGNCC 4 cut(s) 885, 1079, 1401, 3610
BmrI ACTGGG 1 cut(s) 882
BmuI ACTGGG 1 cut(s) 882
BoxI GACNNNNGTC 1 cut(s) 2870
BpiI GAAGAC 3 cut(s) 123, 351, 1173
BpmI CTGGAG 7 cut(s) 416, 1728, 1794, 2665, 3461, 3882, 4053
Bpu10I CCTNAGC 3 cut(s) 249, 287, 3276
Bpu1102I GCTNAGC 1 cut(s) 46
BpuEI CTTGAG 2 cut(s) 3580, 4248
BpuMI CCSGG 5 cut(s) 273, 274, 1068, 1958, 3906
BsaAI YACGTR 1 cut(s) 3323
BsaHI GRCGYC 4 cut(s) 207, 2015, 2113, 2824
BsaWI WCCGGW 3 cut(s) 491, 2020, 3985
BsaXI ACNNNNNCTCC 8 cut(s) 1647, 1677, 2002, 2032, 3880, 3910, 4051, 4081
Bse118I RCCGGY 1 cut(s) 2000
Bse1I ACTGG 8 cut(s) 539, 888, 2546, 3444, 3740, 3765, 3784, 3966
Bse3DI GCAATG 5 cut(s) 459, 1360, 1645, 2541, 3598
BseBI CCWGG 7 cut(s) 434, 2144, 2661, 3295, 3566, 3614, 3704
BseMI GCAATG 5 cut(s) 459, 1360, 1645, 2541, 3598
BseMII CTCAG 8 cut(s) 60, 240, 278, 1856, 2853, 3267, 4146, 4167
BseNI ACTGG 8 cut(s) 539, 888, 2546, 3444, 3740, 3765, 3784, 3966
BseSI GKGCMC 3 cut(s) 1318, 3942, 4262
BseX3I CGGCCG 2 cut(s) 28, 442
BseYI CCCAGC 2 cut(s) 1834, 3346
BsgI GTGCAG 3 cut(s) 686, 1443, 2390
Bsh1236I CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
Bsh1285I CGRYCG 2 cut(s) 31, 445
BshFI GGCC 7 cut(s) 30, 444, 886, 2000, 2734, 3582, 3612
BshNI GGYRCC 1 cut(s) 2112
BsiEI CGRYCG 2 cut(s) 31, 445
BsiHKCI CYCGRG 2 cut(s) 272, 2183
BsiSI CCGG 9 cut(s) 273, 492, 1067, 1371, 1958, 2001, 2021, 3905, 3986
BslFI GGGAC 4 cut(s) 892, 1092, 1481, 1839
BsmAI GTCTC 8 cut(s) 66, 260, 387, 1667, 2021, 2022, 2621, 2858
BsmBI CGTCTC 2 cut(s) 2021, 2022
BsmFI GGGAC 4 cut(s) 892, 1092, 1481, 1839
BsmI GAATGC 4 cut(s) 1165, 1444, 2099, 3169
BsnI GGCC 7 cut(s) 30, 444, 886, 2000, 2734, 3582, 3612
BsoBI CYCGRG 2 cut(s) 272, 2183
Bsp1286I GDGCHC 3 cut(s) 1318, 3942, 4262
Bsp1720I GCTNAGC 1 cut(s) 46
Bsp19I CCATGG 1 cut(s) 3810
Bsp68I TCGCGA 1 cut(s) 3192
BspANI GGCC 7 cut(s) 30, 444, 886, 2000, 2734, 3582, 3612
BspCNI CTCAG 8 cut(s) 59, 241, 279, 1855, 2854, 3268, 4147, 4168
BspFNI CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
BspHI TCATGA 1 cut(s) 1888
BspMAI CTGCAG 4 cut(s) 784, 3384, 3667, 4237
BspQI GCTCTTC 2 cut(s) 2055, 2596
BspT107I GGYRCC 1 cut(s) 2112
BspTI CTTAAG 1 cut(s) 1895
BsrBI CCGCTC 1 cut(s) 456
BsrDI GCAATG 5 cut(s) 459, 1360, 1645, 2541, 3598
BsrFI RCCGGY 1 cut(s) 2000
BsrI ACTGG 8 cut(s) 539, 888, 2546, 3444, 3740, 3765, 3784, 3966
BssAI RCCGGY 1 cut(s) 2000
BssNAI GTATAC 1 cut(s) 3646
BssNI GRCGYC 4 cut(s) 207, 2015, 2113, 2824
BssT1I CCWWGG 6 cut(s) 54, 408, 1201, 2786, 2890, 3810
Bst1107I GTATAC 1 cut(s) 3646
Bst2UI CCWGG 7 cut(s) 434, 2144, 2661, 3295, 3566, 3614, 3704
Bst4CI ACNGT 7 cut(s) 1147, 1252, 1661, 2869, 3022, 4063, 4282
Bst6I CTCTTC 4 cut(s) 58, 305, 2055, 2596
BstACI GRCGYC 4 cut(s) 207, 2015, 2113, 2824
BstAFI CTTAAG 1 cut(s) 1895
BstAPI GCANNNNNTGC 2 cut(s) 2852, 4241
BstBAI YACGTR 1 cut(s) 3323
BstC8I GCNNGC 7 cut(s) 1324, 2002, 2350, 2378, 2613, 2715, 3938
BstDSI CCRYGG 5 cut(s) 31, 910, 1668, 2010, 3810
BstEII GGTNACC 1 cut(s) 1196
BstFNI CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
BstH2I RGCGCY 1 cut(s) 2116
BstHHI GCGC 2 cut(s) 836, 2115
BstMAI GTCTC 8 cut(s) 66, 260, 387, 1667, 2021, 2022, 2621, 2858
BstMCI CGRYCG 2 cut(s) 31, 445
BstNI CCWGG 7 cut(s) 434, 2144, 2661, 3295, 3566, 3614, 3704
BstNSI RCATGY 6 cut(s) 1055, 1326, 2615, 2717, 2881, 3465
BstPAI GACNNNNGTC 1 cut(s) 2870
BstPI GGTNACC 1 cut(s) 1196
BstSFI CTRYAG 7 cut(s) 541, 780, 3380, 3663, 3951, 4233, 4281
BstSLI GKGCMC 3 cut(s) 1318, 3942, 4262
BstUI CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
BstV2I GAAGAC 3 cut(s) 123, 351, 1173
BstX2I RGATCY 8 cut(s) 537, 567, 688, 750, 1952, 2840, 3782, 3962
BstXI CCANNNNNNTGG 2 cut(s) 3710, 3990
BstYI RGATCY 8 cut(s) 537, 567, 688, 750, 1952, 2840, 3782, 3962
BstZ17I GTATAC 1 cut(s) 3646
BstZI CGGCCG 2 cut(s) 28, 442
BsuI GTATCC 2 cut(s) 1027, 2538
BsuRI GGCC 7 cut(s) 30, 444, 886, 2000, 2734, 3582, 3612
BtgI CCRYGG 5 cut(s) 31, 910, 1668, 2010, 3810
BtgZI GCGATG 3 cut(s) 378, 749, 2966
BtsI GCAGTG 6 cut(s) 777, 789, 1579, 3072, 3683, 3882
BtuMI TCGCGA 1 cut(s) 3192
Cac8I GCNNGC 7 cut(s) 1324, 2002, 2350, 2378, 2613, 2715, 3938
CaiI CAGNNNCTG 4 cut(s) 1595, 2852, 4019, 4280
CciI TCATGA 1 cut(s) 1888
CciNI GCGGCCGC 2 cut(s) 28, 442
CfoI GCGC 2 cut(s) 836, 2115
Cfr10I RCCGGY 1 cut(s) 2000
Cfr13I GGNCC 4 cut(s) 885, 1079, 1401, 3610
Cfr42I CCGCGG 1 cut(s) 34
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 5 cut(s) 215, 600, 1799, 2004, 3677
Csp6I GTAC 2 cut(s) 1041, 3097
CspCI CAANNNNNGTGG 2 cut(s) 3817, 3852
CviQI GTAC 2 cut(s) 1041, 3097
DinI GGCGCC 1 cut(s) 2114
DraIII CACNNNGTG 1 cut(s) 2636
DrdI GACNNNNNNGTC 1 cut(s) 401
DriI GACNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 401
EaeI YGGCCR 4 cut(s) 28, 442, 1998, 2732
EagI CGGCCG 2 cut(s) 28, 442
Eam1104I CTCTTC 4 cut(s) 58, 305, 2055, 2596
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 4 cut(s) 58, 305, 2055, 2596
EciI GGCGGA 2 cut(s) 75, 440
EclXI CGGCCG 2 cut(s) 28, 442
Eco130I CCWWGG 6 cut(s) 54, 408, 1201, 2786, 2890, 3810
Eco32I GATATC 1 cut(s) 2257
Eco47I GGWCC 2 cut(s) 1079, 1401
Eco52I CGGCCG 2 cut(s) 28, 442
Eco57I CTGAAG 7 cut(s) 329, 705, 2341, 2577, 2625, 3939, 3996
Eco88I CYCGRG 2 cut(s) 272, 2183
Eco91I GGTNACC 1 cut(s) 1196
EcoO109I RGGNCCY 1 cut(s) 1079
EcoO65I GGTNACC 1 cut(s) 1196
EcoRI GAATTC 2 cut(s) 1361, 3232
EcoRII CCWGG 7 cut(s) 432, 2142, 2659, 3293, 3564, 3612, 3702
EcoRV GATATC 1 cut(s) 2257
EcoT14I CCWWGG 6 cut(s) 54, 408, 1201, 2786, 2890, 3810
EcoT22I ATGCAT 1 cut(s) 1430
EgeI GGCGCC 1 cut(s) 2114
EheI GGCGCC 1 cut(s) 2114
ErhI CCWWGG 6 cut(s) 54, 408, 1201, 2786, 2890, 3810
Esp3I CGTCTC 2 cut(s) 2021, 2022
FalI AAGNNNNNCTT 4 cut(s) 1227, 1259, 2052, 2084
FaqI GGGAC 4 cut(s) 892, 1092, 1481, 1839
FauI CCCGC 1 cut(s) 3295
FauNDI CATATG 4 cut(s) 1135, 2511, 2814, 2950
FbaI TGATCA 2 cut(s) 511, 598
FblI GTMKAC 4 cut(s) 642, 2928, 3283, 3645
GlaI GCGC 2 cut(s) 835, 2114
GsaI CCCAGC 2 cut(s) 1838, 3350
GsuI CTGGAG 7 cut(s) 416, 1728, 1794, 2665, 3461, 3882, 4053
HaeII RGCGCY 1 cut(s) 2116
HaeIII GGCC 7 cut(s) 30, 444, 886, 2000, 2734, 3582, 3612
HapII CCGG 9 cut(s) 273, 492, 1067, 1371, 1958, 2001, 2021, 3905, 3986
HgaI GACGC 5 cut(s) 215, 600, 1799, 2004, 3677
HhaI GCGC 2 cut(s) 836, 2115
Hin1I GRCGYC 4 cut(s) 207, 2015, 2113, 2824
Hin6I GCGC 2 cut(s) 834, 2113
HinP1I GCGC 2 cut(s) 834, 2113
HincII GTYRAC 5 cut(s) 643, 2449, 2752, 2943, 3880
HindII GTYRAC 5 cut(s) 643, 2449, 2752, 2943, 3880
HpaI GTTAAC 2 cut(s) 2752, 2943
HpaII CCGG 9 cut(s) 273, 492, 1067, 1371, 1958, 2001, 2021, 3905, 3986
HphI GGTGA 9 cut(s) 121, 1190, 1217, 1499, 2906, 3113, 3851, 3872, 4061
Hpy99I CGWCG 6 cut(s) 164, 182, 194, 209, 215, 4084
HpyAV CCTTC 9 cut(s) 292, 334, 373, 377, 1474, 1812, 3037, 4182, 4292
HpyCH4III ACNGT 7 cut(s) 1147, 1252, 1661, 2869, 3022, 4063, 4282
HpyCH4IV ACGT 6 cut(s) 2488, 2824, 2936, 3322, 3430, 3537
HpySE526I ACGT 6 cut(s) 2488, 2824, 2936, 3322, 3430, 3537
Hsp92I GRCGYC 4 cut(s) 207, 2015, 2113, 2824
HspAI GCGC 2 cut(s) 834, 2113
KasI GGCGCC 1 cut(s) 2112
KroI GCCGGC 1 cut(s) 2000
KroNI GCCGGC 1 cut(s) 2002
Ksp22I TGATCA 2 cut(s) 511, 598
KspAI GTTAAC 2 cut(s) 2752, 2943
KspI CCGCGG 1 cut(s) 34
LguI GCTCTTC 2 cut(s) 2055, 2596
MaeII ACGT 6 cut(s) 2488, 2824, 2936, 3322, 3430, 3537
MbiI CCGCTC 1 cut(s) 456
MflI RGATCY 8 cut(s) 537, 567, 688, 750, 1952, 2840, 3782, 3962
MhlI GDGCHC 3 cut(s) 1318, 3942, 4262
Mly113I GGCGCC 1 cut(s) 2113
Mph1103I ATGCAT 1 cut(s) 1430
MroNI GCCGGC 1 cut(s) 2000
MroXI GAANNNNTTC 1 cut(s) 2064
MslI CAYNNNNRTG 9 cut(s) 713, 782, 849, 1203, 1333, 1467, 2616, 2817, 3988
MspA1I CMGCKG 7 cut(s) 33, 441, 1838, 1881, 3248, 3346, 4154
MspCI CTTAAG 1 cut(s) 1895
MspI CCGG 9 cut(s) 273, 492, 1067, 1371, 1958, 2001, 2021, 3905, 3986
Mva1269I GAATGC 4 cut(s) 1165, 1444, 2099, 3169
MvaI CCWGG 7 cut(s) 434, 2144, 2661, 3295, 3566, 3614, 3704
MvnI CGCG 7 cut(s) 33, 836, 1300, 1810, 1920, 1950, 3192
NaeI GCCGGC 1 cut(s) 2002
NarI GGCGCC 1 cut(s) 2113
NciI CCSGG 5 cut(s) 273, 274, 1068, 1958, 3906
NcoI CCATGG 1 cut(s) 3810
NdeI CATATG 4 cut(s) 1135, 2511, 2814, 2950
NgoMIV GCCGGC 1 cut(s) 2000
NmeAIII GCCGAG 2 cut(s) 2710, 3064
NmuCI GTSAC 7 cut(s) 775, 890, 1070, 1196, 1505, 1555, 1574
NotI GCGGCCGC 2 cut(s) 28, 442
NruI TCGCGA 1 cut(s) 3192
NsiI ATGCAT 1 cut(s) 1430
NspI RCATGY 6 cut(s) 1055, 1326, 2615, 2717, 2881, 3465
OliI CACNNNNGTG 3 cut(s) 782, 1203, 3988
PaeI GCATGC 3 cut(s) 1326, 2615, 2717
PagI TCATGA 1 cut(s) 1888
PciSI GCTCTTC 2 cut(s) 2055, 2596
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 219, 354
PctI GAATGC 4 cut(s) 1165, 1444, 2099, 3169
PdiI GCCGGC 1 cut(s) 2002
PdmI GAANNNNTTC 1 cut(s) 2064
PfeI GAWTC 9 cut(s) 137, 1945, 2597, 3659, 3923, 4016, 4187, 4201, 4214
PflFI GACNNNGTC 1 cut(s) 209
PflMI CCANNNNNTGG 2 cut(s) 1989, 2972
PfoI TCCNGGA 1 cut(s) 432
PluTI GGCGCC 1 cut(s) 2116
Ppu21I YACGTR 1 cut(s) 3323
PpuMI RGGWCCY 1 cut(s) 1079
PshAI GACNNNNGTC 1 cut(s) 2870
Psp5II RGGWCCY 1 cut(s) 1079
Psp6I CCWGG 7 cut(s) 432, 2142, 2659, 3293, 3564, 3612, 3702
PspEI GGTNACC 1 cut(s) 1196
PspFI CCCAGC 2 cut(s) 1834, 3346
PspGI CCWGG 7 cut(s) 432, 2142, 2659, 3293, 3564, 3612, 3702
PspPI GGNCC 4 cut(s) 885, 1079, 1401, 3610
PspPPI RGGWCCY 1 cut(s) 1079
PstI CTGCAG 4 cut(s) 784, 3384, 3667, 4237
PstNI CAGNNNCTG 4 cut(s) 1595, 2852, 4019, 4280
PsuI RGATCY 8 cut(s) 537, 567, 688, 750, 1952, 2840, 3782, 3962
PsyI GACNNNGTC 1 cut(s) 209
PvuII CAGCTG 4 cut(s) 1838, 3248, 3346, 4154
RruI TCGCGA 1 cut(s) 3192
RsaI GTAC 2 cut(s) 1042, 3098
RsaNI GTAC 2 cut(s) 1041, 3097
RseI CAYNNNNRTG 9 cut(s) 713, 782, 849, 1203, 1333, 1467, 2616, 2817, 3988
SacII CCGCGG 1 cut(s) 34
SalI GTCGAC 1 cut(s) 641
SapI GCTCTTC 2 cut(s) 2055, 2596
Sau96I GGNCC 4 cut(s) 885, 1079, 1401, 3610
SduI GDGCHC 3 cut(s) 1318, 3942, 4262
SfcI CTRYAG 7 cut(s) 541, 780, 3380, 3663, 3951, 4233, 4281
SfoI GGCGCC 1 cut(s) 2114
Sfr303I CCGCGG 1 cut(s) 34
SgrBI CCGCGG 1 cut(s) 34
SinI GGWCC 2 cut(s) 1079, 1401
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 9 cut(s) 713, 782, 849, 1203, 1333, 1467, 2616, 2817, 3988
SmlI CTYRAG 3 cut(s) 1895, 3559, 4263
SmoI CTYRAG 3 cut(s) 1895, 3559, 4263
SphI GCATGC 3 cut(s) 1326, 2615, 2717
SspDI GGCGCC 1 cut(s) 2112
SspI AATATT 1 cut(s) 3307
StyI CCWWGG 6 cut(s) 54, 408, 1201, 2786, 2890, 3810
TaaI ACNGT 7 cut(s) 1147, 1252, 1661, 2869, 3022, 4063, 4282
TaiI ACGT 6 cut(s) 2491, 2827, 2939, 3325, 3433, 3540
TaqI TCGA 8 cut(s) 642, 1141, 1264, 1567, 2161, 3425, 3490, 3574
TauI GCSGC 9 cut(s) 27, 30, 33, 151, 248, 444, 447, 456, 1586
TfiI GAWTC 9 cut(s) 137, 1945, 2597, 3659, 3923, 4016, 4187, 4201, 4214
TseFI GTSAC 7 cut(s) 775, 890, 1070, 1196, 1505, 1555, 1574
Tsp45I GTSAC 7 cut(s) 775, 890, 1070, 1196, 1505, 1555, 1574
TspGWI ACGGA 2 cut(s) 1246, 1301
TspMI CCCGGG 1 cut(s) 272
Tth111I GACNNNGTC 1 cut(s) 209
Van91I CCANNNNNTGG 2 cut(s) 1989, 2972
Vha464I CTTAAG 1 cut(s) 1895
VpaK11BI GGWCC 2 cut(s) 1079, 1401
XapI RAATTY 7 cut(s) 505, 1361, 2213, 2296, 3075, 3232, 3727
XbaI TCTAGA 1 cut(s) 467
XceI RCATGY 6 cut(s) 1055, 1326, 2615, 2717, 2881, 3465
XcmI CCANNNNNNNNNTGG 1 cut(s) 1986
XmaI CCCGGG 1 cut(s) 272
XmaJI CCTAGG 2 cut(s) 2786, 2890
XmiI GTMKAC 4 cut(s) 642, 2928, 3283, 3645
XmnI GAANNNNTTC 1 cut(s) 2064
ZraI GACGTC 1 cut(s) 2825
Zsp2I ATGCAT 1 cut(s) 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.