Rmu_sc0000328.1_g000011

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000328.1
OG0006091
Physical Location & Seq
Forward (+)
74894 .. 76302
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
atgactggcacgcaagcgtgcaggcacatggcgaagtcggcggcgcacgtagcaagtgcgcgcctcttctccacggcagccagagctgccaccactaatgcgcccgcggccaaacaactgtcctccataatcattcctctaaaggatgcggctgcgggcgcatggtggaacaacggcagcattatttaccccgttcagagggtacgcagtggtagcactctggctctcggccgtcaggagaagcagcaggaggaggctgatcatagcaactctgccgtttcggttgccggacacggtggtggtaataaggacgatgaggagaaggccatcaccagctactggggggtgggaccgaacaagattaccagggaagacggtactgaatggaaatggaattgcttcagaccatgggagacgtacaaggccgacacctcgatagatctccagaagcatcatgtgcctacaactttgctggacaaaatggcctactggactgtcaagtctctccgatggcctacggacctattcttccagaggcgatatgggtgcagggcaatgatgctggagacagtggcagcagtgcctggaatggtaggagggatgctgctgcactgcaaatcgcttaggcgattcgagcacagtggcggctggatcaaagctcttctagaagaagccgagaacgagcgtatgcacctcatgaccttcatggaagtcgccaagccccagtggtacgagcgtgcacttgtatttgctgttcaaggtgttttcttcaatgcgtacttcttgggatatatgatttcacccaagtttgctcatagaatggttggatatcttgaggaggaagcaattcactcatataccgagttcctcaatgaattagacaaaggtaacattgagaatgttccagctccagctattgctattgattattggcagctaccttccaattccaccttgagagatgttgttacagttgttagggcagacgaggctcaccaccgagatgtcaatcacttcgcttcggacatacattataatggacgtgaactgaaggaatctccagctccaattggttaccattga

Protein Analysis

360

Amino Acids

40.51

Weight (kDa)

6.98

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 149 - 341 6e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AasI GACNNNNNNGTC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 339
AccII CGCG 2 cut(s) 61, 107
AciI CCGC 6 cut(s) 41, 105, 107, 149, 155, 645
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 2 cut(s) 108, 229
AcuI CTGAAG 2 cut(s) 385, 1070
AfaI GTAC 5 cut(s) 204, 379, 419, 731, 779
AfiI CCNNNNNNNGG 2 cut(s) 198, 339
AgsI TTSAA 2 cut(s) 758, 772
AjiI CACGTC 1 cut(s) 1043
AjnI CCWGG 2 cut(s) 365, 583
AleI CACNNNNGTG 1 cut(s) 297
AluBI AGCT 7 cut(s) 86, 336, 659, 908, 914, 937, 1064
AluI AGCT 7 cut(s) 86, 336, 659, 908, 914, 937, 1064
Alw21I GWGCWC 2 cut(s) 639, 742
Alw26I GTCTC 3 cut(s) 407, 507, 560
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 2 cut(s) 339, 584
AoxI GGCC 6 cut(s) 108, 229, 324, 423, 483, 512
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 9 cut(s) 77, 86, 152, 177, 244, 575, 604, 607, 934
Asp700I GAANNNNTTC 2 cut(s) 398, 846
AspLEI GCGC 5 cut(s) 46, 61, 63, 103, 161
AspS9I GGNCC 2 cut(s) 350, 520
AsuHPI GGTGA 3 cut(s) 322, 792, 986
AvaII GGWCC 2 cut(s) 350, 520
BaeGI GKGCMC 1 cut(s) 742
BaeI ACNNNNGTAYC 2 cut(s) 721, 754
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 2 cut(s) 639, 742
BbvI GCAGC 9 cut(s) 73, 89, 139, 189, 256, 587, 591, 594, 946
BccI CCATC 2 cut(s) 335, 504
BceAI ACGGC 4 cut(s) 90, 190, 216, 260
BcgI CGANNNNNNTGC 2 cut(s) 528, 562
BciT130I CCWGG 2 cut(s) 367, 585
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 3 cut(s) 407, 507, 560
BfaI CTAG 1 cut(s) 665
BglII AGATCT 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 367, 585
Bme18I GGWCC 2 cut(s) 350, 520
BmgBI CACGTC 1 cut(s) 1043
BmgT120I GGNCC 2 cut(s) 350, 520
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 367, 585
BmrI ACTGGG 2 cut(s) 349, 718
BmsI GCATC 4 cut(s) 136, 460, 549, 591
BmuI ACTGGG 2 cut(s) 349, 718
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 4 cut(s) 428, 584, 894, 1044
Bpu10I CCTNAGC 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 854, 976
BsaAI YACGTR 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 1008
BsaJI CCNNGG 4 cut(s) 72, 105, 366, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 339
Bse1I ACTGG 4 cut(s) 10, 344, 494, 724
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 2 cut(s) 367, 585
BseDI CCNNGG 4 cut(s) 72, 105, 366, 407
BseGI GGATG 2 cut(s) 151, 606
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 2 cut(s) 198, 339
BseMI GCAATG 1 cut(s) 561
BseNI ACTGG 4 cut(s) 10, 344, 494, 724
BsePI GCGCGC 1 cut(s) 59
BseRI GAGGAG 3 cut(s) 266, 332, 851
BseSI GKGCMC 1 cut(s) 742
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 9 cut(s) 73, 89, 139, 189, 256, 587, 591, 594, 946
BsgI GTGCAG 3 cut(s) 40, 568, 593
Bsh1236I CGCG 2 cut(s) 61, 107
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 6 cut(s) 110, 231, 326, 425, 485, 514
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 2 cut(s) 639, 742
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 2 cut(s) 198, 339
BsmAI GTCTC 3 cut(s) 407, 507, 560
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 6 cut(s) 110, 231, 326, 425, 485, 514
Bsp1286I GDGCHC 2 cut(s) 639, 742
Bsp143I GATC 3 cut(s) 259, 439, 651
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 6 cut(s) 41, 105, 107, 149, 155, 645
BspANI GGCC 6 cut(s) 110, 231, 326, 425, 485, 514
BspFNI CGCG 2 cut(s) 61, 107
BspHI TCATGA 1 cut(s) 696
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 1 cut(s) 659
BspQI GCTCTTC 1 cut(s) 666
BsrDI GCAATG 1 cut(s) 561
BsrI ACTGG 4 cut(s) 10, 344, 494, 724
BssECI CCNNGG 4 cut(s) 72, 105, 366, 407
BssHII GCGCGC 1 cut(s) 59
BssMI GATC 3 cut(s) 259, 439, 651
BssT1I CCWWGG 1 cut(s) 407
Bst2UI CCWGG 2 cut(s) 367, 585
Bst4CI ACNGT 7 cut(s) 120, 296, 377, 496, 571, 641, 973
Bst6I CTCTTC 2 cut(s) 71, 666
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 8 cut(s) 11, 15, 19, 23, 61, 105, 157, 738
BstDEI CTNAG 1 cut(s) 623
BstDSI CCRYGG 3 cut(s) 72, 105, 407
BstEII GGTNACC 1 cut(s) 1073
BstF5I GGATG 2 cut(s) 151, 606
BstFNI CGCG 2 cut(s) 61, 107
BstHHI GCGC 5 cut(s) 46, 61, 63, 103, 161
BstKTI GATC 3 cut(s) 262, 442, 654
BstMAI GTCTC 3 cut(s) 407, 507, 560
BstMBI GATC 3 cut(s) 259, 439, 651
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 2 cut(s) 367, 585
BstPI GGTNACC 1 cut(s) 1073
BstSCI CCNGG 2 cut(s) 365, 583
BstSLI GKGCMC 1 cut(s) 742
BstUI CGCG 2 cut(s) 61, 107
BstV1I GCAGC 9 cut(s) 73, 89, 139, 189, 256, 587, 591, 594, 946
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 6 cut(s) 110, 231, 326, 425, 485, 514
BtgI CCRYGG 3 cut(s) 72, 105, 407
BtrI CACGTC 1 cut(s) 1043
BtsCI GGATG 2 cut(s) 151, 606
BtsI GCAGTG 3 cut(s) 214, 585, 610
BtsIMutI CAGTG 6 cut(s) 214, 576, 585, 610, 646, 731
Cac8I GCNNGC 8 cut(s) 11, 15, 19, 23, 61, 105, 157, 738
CaiI CAGNNNCTG 2 cut(s) 339, 584
CciI TCATGA 1 cut(s) 696
CfoI GCGC 5 cut(s) 46, 61, 63, 103, 161
Cfr13I GGNCC 2 cut(s) 350, 520
Cfr42I CCGCGG 1 cut(s) 108
Csp6I GTAC 5 cut(s) 203, 378, 418, 730, 778
CviAII CATG 6 cut(s) 28, 162, 408, 455, 697, 706
CviQI GTAC 5 cut(s) 203, 378, 418, 730, 778
DdeI CTNAG 1 cut(s) 623
DpnI GATC 3 cut(s) 261, 441, 653
DpnII GATC 3 cut(s) 259, 439, 651
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 2 cut(s) 108, 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 71, 666
EarI CTCTTC 2 cut(s) 71, 666
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 407
Eco32I GATATC 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 350, 520
Eco52I CGGCCG 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 385, 1070
Eco91I GGTNACC 1 cut(s) 1073
EcoO65I GGTNACC 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 365, 583
EcoRV GATATC 1 cut(s) 830
EcoT14I CCWWGG 1 cut(s) 407
ErhI CCWWGG 1 cut(s) 407
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 6 cut(s) 31, 165, 411, 458, 700, 709
FaqI GGGAC 1 cut(s) 363
FatI CATG 6 cut(s) 27, 161, 407, 454, 696, 705
FauI CCCGC 2 cut(s) 112, 148
FbaI TGATCA 1 cut(s) 259
FokI GGATG 2 cut(s) 158, 613
FspBI CTAG 1 cut(s) 665
GlaI GCGC 5 cut(s) 45, 60, 62, 102, 160
GsuI CTGGAG 4 cut(s) 428, 584, 894, 1044
HaeIII GGCC 6 cut(s) 110, 231, 326, 425, 485, 514
HapII CCGG 1 cut(s) 288
HhaI GCGC 5 cut(s) 46, 61, 63, 103, 161
Hin1II CATG 6 cut(s) 31, 165, 411, 458, 700, 709
Hin6I GCGC 5 cut(s) 44, 59, 61, 101, 159
HinP1I GCGC 5 cut(s) 44, 59, 61, 101, 159
HinfI GANTC 2 cut(s) 630, 1055
HpaII CCGG 1 cut(s) 288
HphI GGTGA 3 cut(s) 322, 792, 986
Hpy166II GTNNAC 2 cut(s) 740, 1046
Hpy188I TCNGA 4 cut(s) 198, 404, 509, 1024
Hpy188III TCNNGA 6 cut(s) 236, 445, 532, 665, 697, 833
Hpy8I GTNNAC 2 cut(s) 740, 1046
HpyAV CCTTC 4 cut(s) 316, 712, 951, 1045
HpyCH4III ACNGT 7 cut(s) 120, 296, 377, 496, 571, 641, 973
HpyCH4IV ACGT 3 cut(s) 48, 416, 1042
HpyCH4V TGCA 6 cut(s) 21, 549, 610, 615, 691, 740
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 3 cut(s) 48, 416, 1042
Hsp92II CATG 6 cut(s) 31, 165, 411, 458, 700, 709
HspAI GCGC 5 cut(s) 44, 59, 61, 101, 159
Ksp22I TGATCA 1 cut(s) 259
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 3 cut(s) 259, 439, 651
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 2 cut(s) 913, 1069
Lsp1109I GCAGC 9 cut(s) 73, 89, 139, 189, 256, 587, 591, 594, 946
LweI GCATC 4 cut(s) 136, 460, 549, 591
MaeI CTAG 1 cut(s) 665
MaeII ACGT 3 cut(s) 48, 416, 1042
MaeIII GTNAC 3 cut(s) 887, 967, 1073
MalI GATC 3 cut(s) 261, 441, 653
MboI GATC 3 cut(s) 259, 439, 651
MboII GAAGA 6 cut(s) 58, 383, 520, 653, 680, 760
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 639, 742
MluCI AATT 5 cut(s) 394, 846, 875, 946, 1068
MmeI TCCRAC 1 cut(s) 805
MroXI GAANNNNTTC 2 cut(s) 398, 846
MslI CAYNNNNRTG 3 cut(s) 297, 1002, 1035
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 367, 585
MunI CAATTG 1 cut(s) 1068
MvaI CCWGG 2 cut(s) 367, 585
MvnI CGCG 2 cut(s) 61, 107
NcoI CCATGG 1 cut(s) 407
NdeII GATC 3 cut(s) 259, 439, 651
NlaIII CATG 6 cut(s) 31, 165, 411, 458, 700, 709
NlaIV GGNNCC 1 cut(s) 351
NmeAIII GCCGAG 2 cut(s) 207, 700
OliI CACNNNNGTG 1 cut(s) 297
PagI TCATGA 1 cut(s) 696
PauI GCGCGC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 666
PdmI GAANNNNTTC 2 cut(s) 398, 846
PfeI GAWTC 2 cut(s) 630, 1055
PflMI CCANNNNNTGG 1 cut(s) 339
Ppu21I YACGTR 1 cut(s) 49
PsiI TTATAA 1 cut(s) 1035
Psp6I CCWGG 2 cut(s) 365, 583
PspEI GGTNACC 1 cut(s) 1073
PspGI CCWGG 2 cut(s) 365, 583
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 2 cut(s) 350, 520
PsrI GAACNNNNNNTAC 2 cut(s) 738, 770
PstNI CAGNNNCTG 2 cut(s) 339, 584
PsuI RGATCY 1 cut(s) 439
PteI GCGCGC 1 cut(s) 59
RsaI GTAC 5 cut(s) 204, 379, 419, 731, 779
RsaNI GTAC 5 cut(s) 203, 378, 418, 730, 778
RseI CAYNNNNRTG 3 cut(s) 297, 1002, 1035
SacII CCGCGG 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 666
Sau3AI GATC 3 cut(s) 259, 439, 651
Sau96I GGNCC 2 cut(s) 350, 520
ScrFI CCNGG 2 cut(s) 367, 585
SduI GDGCHC 2 cut(s) 639, 742
SfaNI GCATC 4 cut(s) 136, 460, 549, 591
Sfr303I CCGCGG 1 cut(s) 108
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 2 cut(s) 350, 520
SmiMI CAYNNNNRTG 3 cut(s) 297, 1002, 1035
SmlI CTYRAG 2 cut(s) 833, 955
SmoI CTYRAG 2 cut(s) 833, 955
Sse9I AATT 5 cut(s) 394, 846, 875, 946, 1068
SsiI CCGC 6 cut(s) 41, 105, 107, 149, 155, 645
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 2 cut(s) 365, 583
StyI CCWWGG 1 cut(s) 407
TaaI ACNGT 7 cut(s) 120, 296, 377, 496, 571, 641, 973
TaiI ACGT 3 cut(s) 51, 419, 1045
TaqI TCGA 2 cut(s) 434, 633
TaqII GACCGA 1 cut(s) 367
TasI AATT 5 cut(s) 394, 846, 875, 946, 1068
TauI GCSGC 4 cut(s) 44, 110, 152, 648
TfiI GAWTC 2 cut(s) 630, 1055
TscAI CASTG 6 cut(s) 214, 576, 585, 617, 646, 731
TseI GCWGC 9 cut(s) 77, 86, 152, 177, 244, 575, 604, 607, 934
TspDTI ATGAA 2 cut(s) 694, 888
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 6 cut(s) 214, 576, 585, 617, 646, 731
Van91I CCANNNNNTGG 1 cut(s) 339
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 350, 520
XbaI TCTAGA 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 2 cut(s) 398, 846
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.