Rmu_sc0000429.1_g000047
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000429.1
OG0009680
Physical Location & Seq
Forward (+)
193763 .. 194257
495 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
atgagagaaccaaaccaagatgcagtagtttccggccgcaaaattggaccaatcatcgatgtttgcaaggaggaaggaattaaaattccagtcccaaccagaactagggacttcccagagattagtaagaagatcaatgataagcttacttatagcctgttggaacttcatttgctgcctgctcgtatttcaactgttgacgaggccttcgccactttcatgcaaattatgacgctgaccaaagaatcgctcagaaaaattcttgtgagaggggaatttgatgagttcctagatgatgagatgcatggcactggaaagctggctgagatgctcaagcagttctcggatgagcttgaccaatttcatcatgattatggtcttcgtgacctcaaaaataacttcttagagaccaagatcaactatatctttaatactcgggatgctgtcaaacaagatcatgtagatgatgcagggaagaacaaagaggagcaatga

Protein Analysis

164

Amino Acids

18.98

Weight (kDa)

5.28

Isoelectric Point (pI)

23.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_M PF01031 38 - 129 4.5e-07 Dynamin central region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 3 cut(s) 84, 258, 275
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 1 cut(s) 192
AluBI AGCT 3 cut(s) 145, 319, 352
AluI AGCT 3 cut(s) 145, 319, 352
Alw26I GTCTC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 435
AoxI GGCC 2 cut(s) 34, 204
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 3 cut(s) 84, 258, 275
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspS9I GGNCC 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 435
AvaII GGWCC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 105, 290
BisI GCNGC 2 cut(s) 37, 176
BlsI GCNGC 2 cut(s) 38, 177
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 47
BmsI GCATC 5 cut(s) 10, 291, 318, 430, 457
BpiI GAAGAC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 401
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 89, 316
BseCI ATCGAT 1 cut(s) 57
BseGI GGATG 2 cut(s) 352, 445
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 265, 315
BseNI ACTGG 2 cut(s) 89, 316
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 36, 206
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 435
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 77, 122
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 77, 122
BsnI GGCC 2 cut(s) 36, 206
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 435
Bsp143I GATC 3 cut(s) 132, 414, 454
BspACI CCGC 1 cut(s) 37
BspANI GGCC 2 cut(s) 36, 206
BspCNI CTCAG 2 cut(s) 264, 316
BspDI ATCGAT 1 cut(s) 57
BspHI TCATGA 1 cut(s) 367
BspTNI GGTCTC 1 cut(s) 401
BsrI ACTGG 2 cut(s) 89, 316
BssMI GATC 3 cut(s) 132, 414, 454
Bst4CI ACNGT 1 cut(s) 196
BstC8I GCNNGC 2 cut(s) 180, 321
BstDEI CTNAG 3 cut(s) 251, 324, 403
BstF5I GGATG 2 cut(s) 352, 445
BstKTI GATC 3 cut(s) 135, 417, 457
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 3 cut(s) 132, 414, 454
BstMCI CGRYCG 1 cut(s) 37
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 371
BstZI CGGCCG 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 57
BsuRI GGCC 2 cut(s) 36, 206
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 2 cut(s) 352, 445
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 180, 321
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 241
CviAII CATG 4 cut(s) 220, 305, 368, 458
CviJI RGCY 7 cut(s) 36, 145, 156, 206, 319, 323, 352
CviKI_1 RGCY 7 cut(s) 36, 145, 156, 206, 319, 323, 352
DdeI CTNAG 3 cut(s) 251, 324, 403
DpnI GATC 3 cut(s) 134, 416, 456
DpnII GATC 3 cut(s) 132, 414, 454
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 34
Eco147I AGGCCT 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 47
Eco52I CGGCCG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 435
EcoT22I ATGCAT 1 cut(s) 306
FaeI CATG 4 cut(s) 223, 308, 371, 461
FaiI YATR 8 cut(s) 153, 221, 230, 306, 369, 375, 423, 459
FaqI GGGAC 2 cut(s) 77, 122
FatI CATG 4 cut(s) 219, 304, 367, 457
Fnu4HI GCNGC 2 cut(s) 37, 176
FokI GGATG 2 cut(s) 359, 452
Fsp4HI GCNGC 2 cut(s) 37, 176
FspBI CTAG 2 cut(s) 105, 290
GluI GCNGC 2 cut(s) 37, 176
HaeIII GGCC 2 cut(s) 36, 206
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 241
Hin1II CATG 4 cut(s) 223, 308, 371, 461
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 1 cut(s) 245
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 2 cut(s) 254, 346
Hpy188III TCNNGA 3 cut(s) 368, 383, 437
Hpy8I GTNNAC 1 cut(s) 199
HpyAV CCTTC 2 cut(s) 68, 217
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 5 cut(s) 23, 66, 223, 304, 470
HpyF3I CTNAG 3 cut(s) 251, 324, 403
Hsp92II CATG 4 cut(s) 223, 308, 371, 461
Kzo9I GATC 3 cut(s) 132, 414, 454
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 9 cut(s) 46, 102, 112, 129, 170, 192, 297, 305, 456
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 5 cut(s) 10, 291, 318, 430, 457
MaeI CTAG 2 cut(s) 105, 290
MaeIII GTNAC 1 cut(s) 383
MalI GATC 3 cut(s) 134, 416, 456
MboI GATC 3 cut(s) 132, 414, 454
MboII GAAGA 3 cut(s) 142, 371, 487
MluCI AATT 7 cut(s) 42, 78, 84, 225, 258, 275, 359
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 5 cut(s) 64, 196, 263, 398, 478
Mph1103I ATGCAT 1 cut(s) 306
MseI TTAA 2 cut(s) 81, 429
MslI CAYNNNNRTG 3 cut(s) 218, 372, 462
MspI CCGG 1 cut(s) 33
NdeII GATC 3 cut(s) 132, 414, 454
NlaIII CATG 4 cut(s) 223, 308, 371, 461
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 306
PagI TCATGA 1 cut(s) 367
PceI AGGCCT 1 cut(s) 206
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 38, 177
PspPI GGNCC 1 cut(s) 47
RseI CAYNNNNRTG 3 cut(s) 218, 372, 462
SaqAI TTAA 2 cut(s) 81, 429
SatI GCNGC 2 cut(s) 37, 176
Sau3AI GATC 3 cut(s) 132, 414, 454
Sau96I GGNCC 1 cut(s) 47
SetI ASST 4 cut(s) 147, 321, 354, 390
SfaNI GCATC 5 cut(s) 10, 291, 318, 430, 457
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 3 cut(s) 218, 372, 462
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 7 cut(s) 42, 78, 84, 225, 258, 275, 359
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 1 cut(s) 37
SspMI CTAG 2 cut(s) 105, 290
StuI AGGCCT 1 cut(s) 206
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 1 cut(s) 57
TasI AATT 7 cut(s) 42, 78, 84, 225, 258, 275, 359
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 81, 429
Tru9I TTAA 2 cut(s) 81, 429
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 3 cut(s) 158, 208, 353
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 3 cut(s) 84, 258, 275
XspI CTAG 2 cut(s) 105, 290
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.