Rmu_sc0000596.1_g000035

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000596.1
OG0004217
Physical Location & Seq
Forward (+)
184904 .. 185508
605 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
atgggaatgaagggagtgagaaagtacttaagtcgaaagatcttcaaaaacattcaaattacgaaggtgggaaacaacgacggaggaattgatgatggttacatgaggactttggagaaattgaagaatgtcaaggggccctcaggtagagtccatgatcagcattttgcagaacaaatgttgctcgaaaaggttaaatgtttgatgacgcggcgagaagatttgcatgaagaattgattagccaaaaaggaaaacctcgcaagaagtatgattgtattcgtgcttggagacgctgtttaaggatggtcactcatatattgttcattggtgctgcagctgctgagtttgcctgcacagttgtggcagcagttatggctgctccacttgttgcactggccattgctgctgctattatcccaacactagccggacagcattggactgatggactctgtcttggattgaagattgtgaaattcctttcgagtatccggatgatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

167

Amino Acids

18.7

Weight (kDa)

9.9

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 23 - 149 5.1e-10 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 211
AccIII TCCGGA 1 cut(s) 492
AciI CCGC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 1 cut(s) 26
AflII CTTAAG 1 cut(s) 28
AgsI TTSAA 4 cut(s) 46, 56, 124, 466
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw26I GTCTC 1 cut(s) 283
AlwNI CAGNNNCTG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 492
AoxI GGCC 2 cut(s) 137, 396
ApaI GGGCCC 1 cut(s) 141
ApeKI GCWGC 7 cut(s) 332, 335, 338, 365, 377, 404, 407
ApoI RAATTY 1 cut(s) 476
AspS9I GGNCC 2 cut(s) 137, 138
AxyI CCTNAGG 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 398
BanII GRGCYC 1 cut(s) 141
BbvI GCAGC 7 cut(s) 319, 325, 347, 364, 377, 391, 394
BccI CCATC 3 cut(s) 89, 298, 440
BciVI GTATCC 1 cut(s) 500
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 28
BfuI GTATCC 1 cut(s) 500
BglII AGATCT 1 cut(s) 39
BisI GCNGC 8 cut(s) 212, 333, 336, 339, 366, 378, 405, 408
BlsI GCNGC 8 cut(s) 213, 334, 337, 340, 367, 379, 406, 409
BmcAI AGTACT 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 137, 138
BmiI GGNNCC 2 cut(s) 138, 139
BsaWI WCCGGW 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bse1I ACTGG 1 cut(s) 399
Bse21I CCTNAGG 1 cut(s) 142
Bse3DI GCAATG 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 492
BseGI GGATG 2 cut(s) 309, 501
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 156, 333
BseNI ACTGG 1 cut(s) 399
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 7 cut(s) 319, 325, 347, 364, 377, 391, 394
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 139, 398
BsiSI CCGG 2 cut(s) 429, 493
BsmAI GTCTC 1 cut(s) 283
BsmBI CGTCTC 1 cut(s) 283
BsnI GGCC 2 cut(s) 139, 398
Bsp120I GGGCCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 492
Bsp143I GATC 2 cut(s) 39, 157
BspACI CCGC 1 cut(s) 211
BspANI GGCC 2 cut(s) 139, 398
BspCNI CTCAG 2 cut(s) 155, 334
BspEI TCCGGA 1 cut(s) 492
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 138, 139
BspMAI CTGCAG 1 cut(s) 337
BspTI CTTAAG 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 1 cut(s) 399
BssMI GATC 2 cut(s) 39, 157
Bst4CI ACNGT 1 cut(s) 358
BstAFI CTTAAG 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 2 cut(s) 142, 342
BstF5I GGATG 2 cut(s) 309, 501
BstFNI CGCG 1 cut(s) 211
BstKTI GATC 2 cut(s) 42, 160
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 2 cut(s) 39, 157
BstMWI GCNNNNNNNGC 4 cut(s) 338, 347, 374, 404
BstSFI CTRYAG 1 cut(s) 333
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 7 cut(s) 319, 325, 347, 364, 377, 391, 394
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
Bsu36I CCTNAGG 1 cut(s) 142
BsuI GTATCC 1 cut(s) 500
BsuRI GGCC 2 cut(s) 139, 398
BtsCI GGATG 2 cut(s) 309, 501
BtsIMutI CAGTG 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 352
CaiI CAGNNNCTG 1 cut(s) 341
Cfr13I GGNCC 2 cut(s) 137, 138
CseI GACGC 2 cut(s) 217, 300
Csp6I GTAC 1 cut(s) 25
CviAII CATG 3 cut(s) 103, 155, 227
CviJI RGCY 6 cut(s) 139, 243, 338, 377, 398, 428
CviKI_1 RGCY 6 cut(s) 139, 243, 338, 377, 398, 428
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 142, 342
DpnI GATC 2 cut(s) 41, 159
DpnII GATC 2 cut(s) 39, 157
EaeI YGGCCR 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 142
EcoO109I RGGNCCY 2 cut(s) 137, 138
EcoT38I GRGCYC 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 3 cut(s) 106, 158, 230
FaiI YATR 7 cut(s) 104, 156, 228, 270, 315, 317, 374
FatI CATG 3 cut(s) 102, 154, 226
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 8 cut(s) 212, 333, 336, 339, 366, 378, 405, 408
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 8 cut(s) 212, 333, 336, 339, 366, 378, 405, 408
FspBI CTAG 1 cut(s) 425
GluI GCNGC 8 cut(s) 212, 333, 336, 339, 366, 378, 405, 408
HaeIII GGCC 2 cut(s) 139, 398
HapII CCGG 2 cut(s) 429, 493
HgaI GACGC 2 cut(s) 217, 300
Hin1II CATG 3 cut(s) 106, 158, 230
HinfI GANTC 2 cut(s) 150, 450
HpaII CCGG 2 cut(s) 429, 493
Hpy188III TCNNGA 1 cut(s) 493
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 4, 58
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 5 cut(s) 170, 226, 335, 354, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 347, 374, 404
HpyF3I CTNAG 2 cut(s) 142, 342
Hsp92II CATG 3 cut(s) 106, 158, 230
Kpn2I TCCGGA 1 cut(s) 492
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 39, 157
LmnI GCTCC 1 cut(s) 385
LpnPI CCDG 4 cut(s) 129, 364, 380, 442
Lsp1109I GCAGC 7 cut(s) 319, 325, 347, 364, 377, 391, 394
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 98, 307
MalI GATC 2 cut(s) 41, 159
MboI GATC 2 cut(s) 39, 157
MboII GAAGA 5 cut(s) 34, 136, 230, 242, 478
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 5 cut(s) 57, 87, 119, 233, 476
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 2 cut(s) 159, 444
MnlI CCTC 4 cut(s) 77, 99, 151, 267
Mox20I TGGCCA 1 cut(s) 398
MroI TCCGGA 1 cut(s) 492
MscI TGGCCA 1 cut(s) 398
MseI TTAA 3 cut(s) 29, 195, 299
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 28
MspI CCGG 2 cut(s) 429, 493
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 4 cut(s) 338, 347, 374, 404
NdeII GATC 2 cut(s) 39, 157
NlaIII CATG 3 cut(s) 106, 158, 230
NlaIV GGNNCC 2 cut(s) 138, 139
NmuCI GTSAC 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 359
PflFI GACNNNGTC 1 cut(s) 453
PkrI GCNGC 8 cut(s) 213, 334, 337, 340, 367, 379, 406, 409
PleI GAGTC 2 cut(s) 158, 444
PpsI GAGTC 2 cut(s) 158, 444
PspN4I GGNNCC 2 cut(s) 138, 139
PspOMI GGGCCC 1 cut(s) 137
PspPI GGNCC 2 cut(s) 137, 138
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 39
PsyI GACNNNGTC 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 3 cut(s) 29, 195, 299
SatI GCNGC 8 cut(s) 212, 333, 336, 339, 366, 378, 405, 408
Sau3AI GATC 2 cut(s) 39, 157
Sau96I GGNCC 2 cut(s) 137, 138
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 2 cut(s) 159, 444
SduI GDGCHC 1 cut(s) 141
SetI ASST 5 cut(s) 69, 148, 195, 259, 340
SfcI CTRYAG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 5 cut(s) 57, 87, 119, 233, 476
SsiI CCGC 1 cut(s) 211
SspMI CTAG 1 cut(s) 425
TaaI ACNGT 1 cut(s) 358
TaqI TCGA 3 cut(s) 34, 186, 485
TasI AATT 5 cut(s) 57, 87, 119, 233, 476
TatI WGTACW 1 cut(s) 24
TauI GCSGC 1 cut(s) 214
Tru1I TTAA 3 cut(s) 29, 195, 299
Tru9I TTAA 3 cut(s) 29, 195, 299
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 7 cut(s) 332, 335, 338, 365, 377, 404, 407
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 23, 243, 313
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 1 cut(s) 399
Tth111I GACNNNGTC 1 cut(s) 453
Vha464I CTTAAG 1 cut(s) 28
XapI RAATTY 1 cut(s) 476
XspI CTAG 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.