Rmu_sc0000657.1_g000021

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000657.1
OG0008107
Physical Location & Seq
Forward (+)
57420 .. 59186
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1767 bp
atgacaatgacccagaagctatcaaaactagttggtttggacctcttgtctgggtcttcaccaacccctcaaaacctcaccaaaatcatatctttctgcgcaaaatcggcttctttcgatgtgggtagcgccattcacgccattgttatcaagttgggtttttgttcgaatgtctatatatgcagtgctctcgtcgacgtgtacggcaaatgtggcgagtcgtggaatgcccagaaggtgttcgacgaaacgcctcacagcaatgtagtgacttggaactctttgatttctggttatttgcaagctggttcgccactgagagcaactcgtttgtttctggacatgttaagggtcggaacagagccgccgacgacgttcagcttttcgggggtgttggtgtgttgcgcacagttagaagctgaagagcttggagctcaggtgcacggtttgagcttgaaaatggggatttgtgatgatgttgtggtgggaacaggattgatagatatgtattcaaagtgttgtaaggtcagagagtcatggcgggtgtttaatcaaatgcgagacaagaatgttgtgacatggacttccatggttactgggtgtgcacagagtgggcagtctgatgaggcaatgactttagtgagagaaatgctgaggttgggtctcaagcctaattatgtcacttataatagtttgttgagttcatttgctagaatggacttttgggattgttgcaggcaaatccattcccggatagtgaaagaagggtttgattccaacgtgtatattgtggttacccttttgaccttatattcagagtctaatagtagcttggaggacttccgtgcgctttgctcatgtgtttcagtgtgggatcaaatctcatggaatgcagtaattgcgggtttttgtaatattgggagcagtgaggaagctctgaagtgtttctctgaaatgaggcaggccggtgttgctgtggattttttcacatttacaagcattttaagagcagcaggaactctttcagcacttgtagagggaaagaaaatccatgctctcatcttcaagagtggacatgcttcaaatttatgtgttcaaaatgggcttgtttcaatgtatggaagatgcggtgccatccatgatgcaaagtgggtgtttactttgatgaaagaacatgatgtggtctcctggaattcattattatcaggttatgcacatcatggatttgggaaggaaactgttgaattgtttgagcaaatgagaagaacagaggtcaaaccaaataataccaccttcctaattgtactaactgcctgtagccatgttggtttgttggacaaagggctcgagtatttcaatctaatgagaaattatgatttgcctgaaccacccaaggtggagcattacgctaccgttgttgatctttttggtcgggctggaaaccttcacgaagcggaagccttcgtcaatagcatgcccatagagccaggtgtctcagtatacaaagctctacttagtgcgtgccaagttcatggaaacagagaacttgctcttcgttctgcaacaaagcttcaacaactgtgcccaaatgatcctgcaacttacatactgctatcaaatgtgttgttgacaagaggctcctgggatgatgcagcagaagtacggaagcacatgtatgatagaggaataagaataactcctggttatagttggatacattcaacattacaatatgaagaaaaggtggatacaacttttaagatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Pkinase_Tyr

Protein Analysis

588

Amino Acids

65.21

Weight (kDa)

6.47

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 88 - 136 1.1e-07 PPR repeat family
PPR PF01535 90 - 119 5.7e-06 PPR repeat
PPR_long PF17177 176 - 268 8.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 189 - 238 5.9e-13 PPR repeat family
PPR_3 PF13812 190 - 236 5.8e-08 Pentatricopeptide repeat domain
PPR PF01535 192 - 222 1.2e-07 PPR repeat
PPR_2 PF13041 292 - 337 3.5e-11 PPR repeat family
PPR PF01535 294 - 324 3.6e-07 PPR repeat
PPR_2 PF13041 393 - 440 8.2e-12 PPR repeat family
PPR PF01535 395 - 422 2e-06 PPR repeat
E_motif PF20431 511 - 572 3.2e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
Acc16I TGCGCA 2 cut(s) 100, 406
AccB1I GGYRCC 1 cut(s) 1130
AccI GTMKAC 2 cut(s) 195, 1500
AciI CCGC 5 cut(s) 365, 541, 902, 1128, 1454
AclWI GGATC 2 cut(s) 882, 1586
AcsI RAATTY 2 cut(s) 1084, 1192
AcuI CTGAAG 2 cut(s) 441, 959
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 3 cut(s) 203, 1305, 1662
AflIII ACRYGT 4 cut(s) 198, 342, 780, 1671
AhdI GACNNNNNGTC 1 cut(s) 46
AhlI ACTAGT 1 cut(s) 28
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 4 cut(s) 1187, 1486, 1640, 1699
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
Alw21I GWGCWC 4 cut(s) 190, 436, 444, 607
Alw26I GTCTC 4 cut(s) 555, 668, 1189, 1498
Alw44I GTGCAC 2 cut(s) 440, 603
AlwI GGATC 2 cut(s) 882, 1586
Ama87I CYCGRG 1 cut(s) 1346
AoxI GGCC 1 cut(s) 963
ApaLI GTGCAC 2 cut(s) 440, 603
ApeKI GCWGC 2 cut(s) 1010, 1652
ApoI RAATTY 2 cut(s) 1084, 1192
Asp700I GAANNNNTTC 3 cut(s) 239, 944, 1021
AspLEI GCGC 4 cut(s) 101, 131, 407, 850
AspS9I GGNCC 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 2 cut(s) 51, 70
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 1346
AvaII GGWCC 1 cut(s) 40
BaeGI GKGCMC 3 cut(s) 444, 607, 1586
BanI GGYRCC 1 cut(s) 1130
BanII GRGCYC 2 cut(s) 436, 1347
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 4 cut(s) 190, 436, 444, 607
BbvCI CCTCAGC 1 cut(s) 653
BbvI GCAGC 2 cut(s) 1022, 1664
BccI CCATC 1 cut(s) 1142
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 4 cut(s) 1189, 1488, 1642, 1701
BciVI GTATCC 2 cut(s) 1707, 1741
BcnI CCSGG 1 cut(s) 751
BcoDI GTCTC 4 cut(s) 555, 668, 1189, 1498
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 2 cut(s) 29, 711
BfmI CTRYAG 1 cut(s) 1315
BfoI RGCGCY 1 cut(s) 132
BfuI GTATCC 2 cut(s) 1707, 1741
BisI GCNGC 3 cut(s) 365, 1011, 1653
BlsI GCNGC 3 cut(s) 366, 1012, 1654
Bme1390I CCNGG 5 cut(s) 751, 1189, 1488, 1642, 1701
Bme18I GGWCC 1 cut(s) 40
BmeRI GACNNNNNGTC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 1346
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 2 cut(s) 1132, 1639
BmrFI CCNGG 5 cut(s) 751, 1189, 1488, 1642, 1701
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 3 cut(s) 1115, 1132, 1639
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 310, 342
Bpu10I CCTNAGC 2 cut(s) 435, 653
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 751
BsaI GGTCTC 2 cut(s) 668, 1189
BsaJI CCNNGG 3 cut(s) 588, 1392, 1641
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse118I RCCGGY 1 cut(s) 965
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 2 cut(s) 268, 636
BseBI CCWGG 4 cut(s) 1189, 1488, 1642, 1701
BseDI CCNNGG 3 cut(s) 588, 1392, 1641
BseGI GGATG 2 cut(s) 1134, 1651
BseMI GCAATG 2 cut(s) 268, 636
BseMII CTCAG 4 cut(s) 308, 449, 644, 1509
BseNI ACTGG 1 cut(s) 601
BseSI GKGCMC 3 cut(s) 444, 607, 1586
BseXI GCAGC 2 cut(s) 1022, 1664
BshFI GGCC 1 cut(s) 965
BshNI GGYRCC 1 cut(s) 1130
BsiHKAI GWGCWC 4 cut(s) 190, 436, 444, 607
BsiHKCI CYCGRG 1 cut(s) 1346
BsiSI CCGG 2 cut(s) 751, 966
BsmAI GTCTC 4 cut(s) 555, 668, 1189, 1498
BsmI GAATGC 2 cut(s) 232, 895
BsnI GGCC 1 cut(s) 965
Bso31I GGTCTC 2 cut(s) 668, 1189
BsoBI CYCGRG 1 cut(s) 1346
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 6 cut(s) 190, 436, 444, 607, 1347, 1586
Bsp143I GATC 3 cut(s) 874, 1420, 1591
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 5 cut(s) 365, 541, 902, 1128, 1454
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 4 cut(s) 309, 448, 645, 1508
BspLI GGNNCC 2 cut(s) 1132, 1639
BspPI GGATC 2 cut(s) 882, 1586
BspQI GCTCTTC 2 cut(s) 417, 1557
BspT104I TTCGAA 1 cut(s) 167
BspT107I GGYRCC 1 cut(s) 1130
BspTNI GGTCTC 2 cut(s) 668, 1189
BsrDI GCAATG 2 cut(s) 268, 636
BsrFI RCCGGY 1 cut(s) 965
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 1 cut(s) 965
BssECI CCNNGG 3 cut(s) 588, 1392, 1641
BssMI GATC 3 cut(s) 874, 1420, 1591
BssNAI GTATAC 1 cut(s) 1501
BssT1I CCWWGG 2 cut(s) 588, 1392
Bst1107I GTATAC 1 cut(s) 1501
Bst2UI CCWGG 4 cut(s) 1189, 1488, 1642, 1701
Bst4CI ACNGT 5 cut(s) 411, 446, 1240, 1414, 1581
Bst6I CTCTTC 2 cut(s) 417, 1557
BstAPI GCANNNNNTGC 1 cut(s) 899
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 5 cut(s) 303, 737, 963, 1475, 1522
BstDEI CTNAG 5 cut(s) 317, 435, 653, 1495, 1514
BstDSI CCRYGG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 793
BstF5I GGATG 2 cut(s) 1134, 1651
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 4 cut(s) 101, 131, 407, 850
BstKTI GATC 3 cut(s) 877, 1423, 1594
BstMAI GTCTC 4 cut(s) 555, 668, 1189, 1498
BstMBI GATC 3 cut(s) 874, 1420, 1591
BstMWI GCNNNNNNNGC 6 cut(s) 107, 137, 213, 899, 971, 1483
BstNI CCWGG 4 cut(s) 1189, 1488, 1642, 1701
BstNSI RCATGY 4 cut(s) 346, 1079, 1477, 1675
BstPI GGTNACC 1 cut(s) 793
BstSCI CCNGG 5 cut(s) 749, 1187, 1486, 1640, 1699
BstSFI CTRYAG 1 cut(s) 1315
BstSLI GKGCMC 3 cut(s) 444, 607, 1586
BstV1I GCAGC 2 cut(s) 1022, 1664
BstV2I GAAGAC 1 cut(s) 48
BstXI CCANNNNNNTGG 1 cut(s) 1532
BstZ17I GTATAC 1 cut(s) 1501
BsuI GTATCC 2 cut(s) 1707, 1741
BsuRI GGCC 1 cut(s) 965
BtgI CCRYGG 1 cut(s) 588
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 2 cut(s) 1134, 1651
BtsI GCAGTG 2 cut(s) 190, 931
BtsIMutI CAGTG 4 cut(s) 190, 314, 873, 931
Cac8I GCNNGC 5 cut(s) 303, 737, 963, 1475, 1522
CfoI GCGC 4 cut(s) 101, 131, 407, 850
Cfr10I RCCGGY 1 cut(s) 965
Cfr13I GGNCC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 202, 1304, 1661
CviQI GTAC 3 cut(s) 202, 1304, 1661
DdeI CTNAG 5 cut(s) 317, 435, 653, 1495, 1514
DpnI GATC 3 cut(s) 876, 1422, 1593
DpnII GATC 3 cut(s) 874, 1420, 1591
DraIII CACNNNGTG 1 cut(s) 611
DriI GACNNNNNGTC 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 417, 1557
Eam1105I GACNNNNNGTC 1 cut(s) 46
EarI CTCTTC 2 cut(s) 417, 1557
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 2 cut(s) 588, 1392
Eco24I GRGCYC 2 cut(s) 436, 1347
Eco31I GGTCTC 2 cut(s) 668, 1189
Eco47I GGWCC 1 cut(s) 40
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 2 cut(s) 441, 959
Eco88I CYCGRG 1 cut(s) 1346
Eco91I GGTNACC 1 cut(s) 793
EcoICRI GAGCTC 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 793
EcoRI GAATTC 1 cut(s) 1192
EcoRII CCWGG 4 cut(s) 1187, 1486, 1640, 1699
EcoT14I CCWWGG 2 cut(s) 588, 1392
EcoT38I GRGCYC 2 cut(s) 436, 1347
ErhI CCWWGG 2 cut(s) 588, 1392
FalI AAGNNNNNCTT 2 cut(s) 1497, 1529
FauI CCCGC 2 cut(s) 534, 895
FblI GTMKAC 2 cut(s) 195, 1500
Fnu4HI GCNGC 3 cut(s) 365, 1011, 1653
FokI GGATG 2 cut(s) 1121, 1658
FriOI GRGCYC 2 cut(s) 436, 1347
Fsp4HI GCNGC 3 cut(s) 365, 1011, 1653
FspBI CTAG 2 cut(s) 29, 711
FspI TGCGCA 2 cut(s) 100, 406
GlaI GCGC 4 cut(s) 100, 130, 406, 849
GluI GCNGC 3 cut(s) 365, 1011, 1653
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 965
HapII CCGG 2 cut(s) 751, 966
HhaI GCGC 4 cut(s) 101, 131, 407, 850
Hin6I GCGC 4 cut(s) 99, 129, 405, 848
HinP1I GCGC 4 cut(s) 99, 129, 405, 848
HincII GTYRAC 2 cut(s) 196, 1629
HindII GTYRAC 2 cut(s) 196, 1629
HindIII AAGCTT 1 cut(s) 1568
HinfI GANTC 4 cut(s) 218, 533, 773, 818
HpaII CCGG 2 cut(s) 751, 966
HphI GGTGA 2 cut(s) 51, 70
Hpy166II GTNNAC 8 cut(s) 196, 202, 442, 605, 1073, 1158, 1501, 1629
Hpy188I TCNGA 6 cut(s) 356, 530, 622, 817, 939, 952
Hpy188III TCNNGA 3 cut(s) 338, 1066, 1448
Hpy8I GTNNAC 8 cut(s) 196, 202, 442, 605, 1073, 1158, 1501, 1629
Hpy99I CGWCG 5 cut(s) 197, 200, 248, 373, 376
HpyAV CCTTC 6 cut(s) 229, 758, 1225, 1303, 1454, 1471
HpyCH4III ACNGT 5 cut(s) 411, 446, 1240, 1414, 1581
HpyCH4IV ACGT 3 cut(s) 198, 374, 780
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 137, 213, 899, 971, 1483
HpyF3I CTNAG 5 cut(s) 317, 435, 653, 1495, 1514
HpySE526I ACGT 3 cut(s) 198, 374, 780
HspAI GCGC 4 cut(s) 99, 129, 405, 848
Kzo9I GATC 3 cut(s) 874, 1420, 1591
LguI GCTCTTC 2 cut(s) 417, 1557
LmnI GCTCC 4 cut(s) 431, 921, 1399, 1643
Lsp1109I GCAGC 2 cut(s) 1022, 1664
LweI GCATC 3 cut(s) 1115, 1132, 1639
MaeI CTAG 2 cut(s) 29, 711
MaeII ACGT 3 cut(s) 198, 374, 780
MaeIII GTNAC 5 cut(s) 268, 574, 592, 679, 793
MalI GATC 3 cut(s) 876, 1422, 1593
MboI GATC 3 cut(s) 874, 1420, 1591
MboII GAAGA 7 cut(s) 48, 434, 1054, 1134, 1275, 1544, 1748
MhlI GDGCHC 6 cut(s) 190, 436, 444, 607, 1347, 1586
MluCI AATT 7 cut(s) 673, 897, 1084, 1192, 1244, 1299, 1369
MlyI GAGTC 3 cut(s) 227, 542, 827
MmeI TCCRAC 4 cut(s) 334, 801, 1314, 1691
MroXI GAANNNNTTC 3 cut(s) 239, 944, 1021
MseI TTAA 4 cut(s) 347, 549, 1004, 1758
MslI CAYNNNNRTG 2 cut(s) 261, 1674
MspI CCGG 2 cut(s) 751, 966
MspR9I CCNGG 5 cut(s) 751, 1189, 1488, 1642, 1701
Mva1269I GAATGC 2 cut(s) 232, 895
MvaI CCWGG 4 cut(s) 1189, 1488, 1642, 1701
MwoI GCNNNNNNNGC 6 cut(s) 107, 137, 213, 899, 971, 1483
NciI CCSGG 1 cut(s) 751
NcoI CCATGG 1 cut(s) 588
NdeII GATC 3 cut(s) 874, 1420, 1591
NlaIV GGNNCC 2 cut(s) 1132, 1639
NmuCI GTSAC 3 cut(s) 268, 574, 679
NsbI TGCGCA 2 cut(s) 100, 406
NspI RCATGY 4 cut(s) 346, 1079, 1477, 1675
NspV TTCGAA 1 cut(s) 167
PaeI GCATGC 1 cut(s) 1477
PaeR7I CTCGAG 1 cut(s) 1346
PciI ACATGT 2 cut(s) 342, 1671
PciSI GCTCTTC 2 cut(s) 417, 1557
PctI GAATGC 2 cut(s) 232, 895
PdmI GAANNNNTTC 3 cut(s) 239, 944, 1021
PfeI GAWTC 1 cut(s) 773
PfoI TCCNGGA 2 cut(s) 749, 1187
PkrI GCNGC 3 cut(s) 366, 1012, 1654
PleI GAGTC 3 cut(s) 226, 541, 826
PpsI GAGTC 3 cut(s) 226, 541, 826
PscI ACATGT 2 cut(s) 342, 1671
PsiI TTATAA 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 4 cut(s) 1187, 1486, 1640, 1699
PspEI GGTNACC 1 cut(s) 793
PspGI CCWGG 4 cut(s) 1187, 1486, 1640, 1699
PspN4I GGNNCC 2 cut(s) 1132, 1639
PspPI GGNCC 1 cut(s) 40
PspXI VCTCGAGB 1 cut(s) 1346
RsaI GTAC 3 cut(s) 203, 1305, 1662
RsaNI GTAC 3 cut(s) 202, 1304, 1661
RseI CAYNNNNRTG 2 cut(s) 261, 1674
SacI GAGCTC 1 cut(s) 436
SalI GTCGAC 1 cut(s) 194
SapI GCTCTTC 2 cut(s) 417, 1557
SaqAI TTAA 4 cut(s) 347, 549, 1004, 1758
SatI GCNGC 3 cut(s) 365, 1011, 1653
Sau3AI GATC 3 cut(s) 874, 1420, 1591
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 3 cut(s) 227, 542, 827
ScrFI CCNGG 5 cut(s) 751, 1189, 1488, 1642, 1701
SduI GDGCHC 6 cut(s) 190, 436, 444, 607, 1347, 1586
SfaNI GCATC 3 cut(s) 1115, 1132, 1639
SfcI CTRYAG 1 cut(s) 1315
Sfr274I CTCGAG 1 cut(s) 1346
SfuI TTCGAA 1 cut(s) 167
SgrDI CGTCGACG 1 cut(s) 194
SinI GGWCC 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 1346
SmiMI CAYNNNNRTG 2 cut(s) 261, 1674
SmlI CTYRAG 2 cut(s) 665, 1346
SmoI CTYRAG 2 cut(s) 665, 1346
SpeI ACTAGT 1 cut(s) 28
SphI GCATGC 1 cut(s) 1477
Sse9I AATT 7 cut(s) 673, 897, 1084, 1192, 1244, 1299, 1369
SsiI CCGC 5 cut(s) 365, 541, 902, 1128, 1454
SspI AATATT 1 cut(s) 916
SspMI CTAG 2 cut(s) 29, 711
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 5 cut(s) 749, 1187, 1486, 1640, 1699
StyI CCWWGG 2 cut(s) 588, 1392
TaaI ACNGT 5 cut(s) 411, 446, 1240, 1414, 1581
TaiI ACGT 3 cut(s) 201, 377, 783
TaqI TCGA 5 cut(s) 117, 167, 195, 243, 1347
TasI AATT 7 cut(s) 673, 897, 1084, 1192, 1244, 1299, 1369
TatI WGTACW 1 cut(s) 1303
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 4 cut(s) 347, 549, 1004, 1758
Tru9I TTAA 4 cut(s) 347, 549, 1004, 1758
TscAI CASTG 4 cut(s) 190, 321, 873, 931
TseFI GTSAC 3 cut(s) 268, 574, 679
TseI GCWGC 2 cut(s) 1010, 1652
Tsp45I GTSAC 3 cut(s) 268, 574, 679
TspDTI ATGAA 5 cut(s) 693, 1181, 1185, 1520, 1749
TspGWI ACGGA 2 cut(s) 833, 1678
TspRI CASTG 4 cut(s) 190, 321, 873, 931
VneI GTGCAC 2 cut(s) 440, 603
VpaK11BI GGWCC 1 cut(s) 40
XapI RAATTY 2 cut(s) 1084, 1192
XceI RCATGY 4 cut(s) 346, 1079, 1477, 1675
XhoI CTCGAG 1 cut(s) 1346
XmiI GTMKAC 2 cut(s) 195, 1500
XmnI GAANNNNTTC 3 cut(s) 239, 944, 1021
XspI CTAG 2 cut(s) 29, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.