Rmu_sc0000762.1_g000066

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000762.1
OG0006964
Physical Location & Seq
Forward (+)
242720 .. 243830
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
atgctcctcagctatgagctcccaatcggtatcactcatatcgtccgacctgtaggccatgatgaagagccagagagactttctggtgaggtggaggaagaggtgatggcggcggtggacggcctcgtcgaagagcccgacaccgtgacgactttgttgtgtagatcgcgccatgtctccaagtcgtcactggcgattgtgaggtatgcaacgacatcggtttgtcagtcgcgccttgcctctaggtcgtcgccggcgagcaacggcattgaaggtgaagatggtggtgcaagatctgatagtgccgtaccagtagtcgcgggaatcagaggtggagacaacgacgtgaaggcggtggcgctcgaattggagcaacggagaaatagaagagaagctttctcgttgaaattggcgaggagcctgacctgccgcctcgtccaaacggcgtcaccgacgtcttcaccgtcggagaagagcaaagagatcatggagtgtagaaggaaactggggcacattgtgttgccagtattctatcatgttgatccttcagatgtgaggaaacagagaggtagttttgcagaagcttttcataaaaaaaaaaaacatcatgataaaggtgaagacaaggtaaaaaggtggagagctgctcttactgaagctacaaatttatctggatgggaccttagaaacaccgccgatgggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

234

Amino Acids

25.75

Weight (kDa)

8.34

Isoelectric Point (pI)

67.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 159 - 231 1.3e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 434
AccII CGCG 3 cut(s) 169, 232, 320
AciI CCGC 6 cut(s) 110, 113, 320, 353, 430, 693
AclWI GGATC 1 cut(s) 536
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 2 cut(s) 531, 675
AcyI GRCGYC 2 cut(s) 446, 455
AdeI CACNNNGTG 1 cut(s) 517
AfaI GTAC 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 699
AgsI TTSAA 2 cut(s) 272, 406
AjiI CACGTC 1 cut(s) 346
AluBI AGCT 6 cut(s) 12, 19, 395, 584, 644, 659
AluI AGCT 6 cut(s) 12, 19, 395, 584, 644, 659
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 70, 181, 330
AlwI GGATC 1 cut(s) 536
AoxI GGCC 2 cut(s) 55, 121
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 1 cut(s) 664
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 3 cut(s) 171, 234, 361
AspS9I GGNCC 1 cut(s) 679
AsuHPI GGTGA 6 cut(s) 98, 115, 287, 441, 453, 629
AvaII GGWCC 1 cut(s) 679
BaeGI GKGCMC 1 cut(s) 513
BanII GRGCYC 2 cut(s) 21, 138
BbsI GAAGAC 2 cut(s) 450, 627
Bbv12I GWGCWC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 631
BccI CCATC 4 cut(s) 100, 275, 669, 692
BceAI ACGGC 4 cut(s) 136, 280, 290, 459
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BcoDI GTCTC 3 cut(s) 70, 181, 330
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 434
BglII AGATCT 1 cut(s) 293
BisI GCNGC 3 cut(s) 111, 430, 645
BlsI GCNGC 3 cut(s) 112, 431, 646
Bme18I GGWCC 1 cut(s) 679
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 679
BmiI GGNNCC 2 cut(s) 419, 680
BmrI ACTGGG 1 cut(s) 515
BmuI ACTGGG 1 cut(s) 515
BpiI GAAGAC 2 cut(s) 450, 627
BplI GAGNNNNNCTC 2 cut(s) 631, 663
Bpu10I CCTNAGC 1 cut(s) 8
BsaHI GRCGYC 2 cut(s) 446, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 4 cut(s) 195, 311, 510, 524
BseGI GGATG 1 cut(s) 680
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 4 cut(s) 195, 311, 510, 524
BseRI GAGGAG 1 cut(s) 430
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 1 cut(s) 631
Bsh1236I CGCG 3 cut(s) 169, 232, 320
BshFI GGCC 2 cut(s) 57, 123
BsiHKAI GWGCWC 1 cut(s) 21
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 1 cut(s) 699
BsmAI GTCTC 3 cut(s) 70, 181, 330
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 2 cut(s) 57, 123
Bsp1286I GDGCHC 3 cut(s) 21, 138, 513
Bsp143I GATC 4 cut(s) 164, 293, 483, 541
BspACI CCGC 6 cut(s) 110, 113, 320, 353, 430, 693
BspANI GGCC 2 cut(s) 57, 123
BspCNI CTCAG 1 cut(s) 21
BspFNI CGCG 3 cut(s) 169, 232, 320
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 419, 680
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 1 cut(s) 536
BspQI GCTCTTC 3 cut(s) 60, 126, 467
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 4 cut(s) 195, 311, 510, 524
BssAI RCCGGY 1 cut(s) 253
BssMI GATC 4 cut(s) 164, 293, 483, 541
BssNI GRCGYC 2 cut(s) 446, 455
Bst4CI ACNGT 2 cut(s) 145, 465
Bst6I CTCTTC 5 cut(s) 60, 93, 126, 382, 467
BstACI GRCGYC 2 cut(s) 446, 455
BstC8I GCNNGC 2 cut(s) 255, 259
BstDEI CTNAG 2 cut(s) 8, 683
BstF5I GGATG 1 cut(s) 680
BstFNI CGCG 3 cut(s) 169, 232, 320
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 3 cut(s) 171, 234, 361
BstKTI GATC 4 cut(s) 167, 296, 486, 544
BstMAI GTCTC 3 cut(s) 70, 181, 330
BstMBI GATC 4 cut(s) 164, 293, 483, 541
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 51
BstSLI GKGCMC 1 cut(s) 513
BstUI CGCG 3 cut(s) 169, 232, 320
BstV1I GCAGC 1 cut(s) 631
BstV2I GAAGAC 2 cut(s) 450, 627
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
BsuRI GGCC 2 cut(s) 57, 123
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 680
BtsIMutI CAGTG 1 cut(s) 188
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 255, 259
CciI TCATGA 1 cut(s) 607
CfoI GCGC 3 cut(s) 171, 234, 361
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 679
CseI GACGC 1 cut(s) 435
Csp6I GTAC 1 cut(s) 308
CviAII CATG 5 cut(s) 59, 173, 487, 536, 608
CviQI GTAC 1 cut(s) 308
DdeI CTNAG 2 cut(s) 8, 683
DpnI GATC 4 cut(s) 166, 295, 485, 543
DpnII GATC 4 cut(s) 164, 293, 483, 541
DraIII CACNNNGTG 1 cut(s) 517
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 5 cut(s) 60, 93, 126, 382, 467
EarI CTCTTC 5 cut(s) 60, 93, 126, 382, 467
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 21, 138
Eco47I GGWCC 1 cut(s) 679
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 531, 675
EcoICRI GAGCTC 1 cut(s) 19
EcoO109I RGGNCCY 1 cut(s) 679
EcoT38I GRGCYC 2 cut(s) 21, 138
FaeI CATG 5 cut(s) 62, 176, 490, 539, 611
FaiI YATR 9 cut(s) 15, 39, 60, 174, 207, 488, 537, 591, 609
FalI AAGNNNNNCTT 2 cut(s) 379, 411
FaqI GGGAC 1 cut(s) 692
FatI CATG 5 cut(s) 58, 172, 486, 535, 607
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 111, 430, 645
FokI GGATG 1 cut(s) 687
FriOI GRGCYC 2 cut(s) 21, 138
Fsp4HI GCNGC 3 cut(s) 111, 430, 645
FspBI CTAG 1 cut(s) 243
GlaI GCGC 3 cut(s) 170, 233, 360
GluI GCNGC 3 cut(s) 111, 430, 645
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 2 cut(s) 57, 123
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 435
HhaI GCGC 3 cut(s) 171, 234, 361
Hin1I GRCGYC 2 cut(s) 446, 455
Hin1II CATG 5 cut(s) 62, 176, 490, 539, 611
Hin6I GCGC 3 cut(s) 169, 232, 359
HinP1I GCGC 3 cut(s) 169, 232, 359
HindIII AAGCTT 2 cut(s) 393, 582
HinfI GANTC 1 cut(s) 324
HpaII CCGG 1 cut(s) 254
HphI GGTGA 6 cut(s) 98, 115, 287, 441, 453, 629
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 5 cut(s) 47, 298, 329, 469, 550
Hpy188III TCNNGA 2 cut(s) 608, 672
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 5 cut(s) 131, 253, 347, 457, 469
HpyAV CCTTC 4 cut(s) 266, 343, 492, 555
HpyCH4III ACNGT 2 cut(s) 145, 465
HpyCH4IV ACGT 2 cut(s) 345, 455
HpyCH4V TGCA 3 cut(s) 209, 290, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 2 cut(s) 8, 683
HpySE526I ACGT 2 cut(s) 345, 455
Hsp92I GRCGYC 2 cut(s) 446, 455
Hsp92II CATG 5 cut(s) 62, 176, 490, 539, 611
HspAI GCGC 3 cut(s) 169, 232, 359
KroI GCCGGC 1 cut(s) 253
KroNI GCCGGC 1 cut(s) 255
Kzo9I GATC 4 cut(s) 164, 293, 483, 541
LguI GCTCTTC 3 cut(s) 60, 126, 467
LmnI GCTCC 4 cut(s) 9, 24, 370, 417
Lsp1109I GCAGC 1 cut(s) 631
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 345, 455
MaeIII GTNAC 3 cut(s) 145, 186, 447
MalI GATC 4 cut(s) 166, 295, 485, 543
MboI GATC 4 cut(s) 164, 293, 483, 541
MboII GAAGA 8 cut(s) 77, 110, 143, 290, 399, 450, 484, 632
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 3 cut(s) 21, 138, 513
MluCI AATT 3 cut(s) 365, 407, 664
MmeI TCCRAC 2 cut(s) 70, 447
MreI CGCCGGCG 1 cut(s) 253
MroNI GCCGGC 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 585
MspI CCGG 1 cut(s) 254
MvnI CGCG 3 cut(s) 169, 232, 320
MwoI GCNNNNNNNGC 1 cut(s) 426
NaeI GCCGGC 1 cut(s) 255
NdeII GATC 4 cut(s) 164, 293, 483, 541
NgoMIV GCCGGC 1 cut(s) 253
NlaIII CATG 5 cut(s) 62, 176, 490, 539, 611
NlaIV GGNNCC 2 cut(s) 419, 680
NmuCI GTSAC 3 cut(s) 145, 186, 447
PagI TCATGA 1 cut(s) 607
PciSI GCTCTTC 3 cut(s) 60, 126, 467
PcsI WCGNNNNNNNCGW 6 cut(s) 126, 135, 191, 254, 449, 461
PdiI GCCGGC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 3 cut(s) 112, 431, 646
PpuMI RGGWCCY 1 cut(s) 679
Psp124BI GAGCTC 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 679
PspN4I GGNNCC 2 cut(s) 419, 680
PspPI GGNCC 1 cut(s) 679
PspPPI RGGWCCY 1 cut(s) 679
PsuI RGATCY 1 cut(s) 293
RsaI GTAC 1 cut(s) 309
RsaNI GTAC 1 cut(s) 308
SacI GAGCTC 1 cut(s) 21
SapI GCTCTTC 3 cut(s) 60, 126, 467
SatI GCNGC 3 cut(s) 111, 430, 645
Sau3AI GATC 4 cut(s) 164, 293, 483, 541
Sau96I GGNCC 1 cut(s) 679
SduI GDGCHC 3 cut(s) 21, 138, 513
SfcI CTRYAG 1 cut(s) 51
SgrAI CRCCGGYG 1 cut(s) 253
SinI GGWCC 1 cut(s) 679
Sse9I AATT 3 cut(s) 365, 407, 664
SsiI CCGC 6 cut(s) 110, 113, 320, 353, 430, 693
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 21
TaaI ACNGT 2 cut(s) 145, 465
TaiI ACGT 2 cut(s) 348, 458
TaqI TCGA 2 cut(s) 129, 363
TasI AATT 3 cut(s) 365, 407, 664
TauI GCSGC 2 cut(s) 113, 432
TfiI GAWTC 1 cut(s) 324
TscAI CASTG 1 cut(s) 195
TseFI GTSAC 3 cut(s) 145, 186, 447
TseI GCWGC 1 cut(s) 644
Tsp45I GTSAC 3 cut(s) 145, 186, 447
TspDTI ATGAA 2 cut(s) 78, 578
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 679
XapI RAATTY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 243
ZraI GACGTC 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.