Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000795.1

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Reverse (-)

234598 .. 240147

Gene Structure 5550 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

atggatgatagtctatacgacgagttcgggaactatataggacccgaaatcgagtctgaccaggagagtgatagggaggaagaagatgaggagcttccggataggttagaagatagaggtgcggcatctgacgcggaggaggtgtacggtgaagatgtcgagacgttggtcatggatgaagatgcacagccccttgaacagctgattatcaagccagttcggaatattaagtttgaggttggtgtcaaggactcctcgacgtatgtgtcgacgcagtttcttgttgggcttatgtcgaaccctgctctggtttggaatgtggcgctggtggggcacttgcagcatgggaagacggtgtttatggatatgttggtagaacagactcatcatatgtctacttttgatgcaaatagcgataagcacatgaggtacactgatagtctgatacgaggattgatgaacaagagaggaggatatctattaaggctgttcccatgtcccttgtacttgaggatagcaattcaaagtcgtacctgtgcaatgtcatggatactcctggtcataagggatgaggtaaatactgagagggctatatgccatgcaatccaagagcgcctaccaattgtggttgtaatcaacaaggttgacaggttgatgacagaacttaaattgcccccaagtgatgcttacttcaagcttaggcatactctggaagtgattaataaccacataacggctgcctcttcaactgctggtcatgtacaagttattgatcctgttgctggaaacgtatgctttgcaagtgctactgcaggatggtcgttcacgttgcaatcatttgcaaagctatatatgttaagcttcatgggatcgcttttgacgcggatcagtttacttctcatctatggggagatatgtgctatcatcctgacgacagggctttcaggagaaaaccccctgcaagtggaggagatagaaaggtcgtttgtccagtttgtgcttgaacccctgtataaaatttatagccaggtgattggagaacataaaaagagtgttgaggcaactcttgctgaacttggtgtcacccttagcaatgcagcttacaaattgaatgttaggcctctactgaggttggcttgtagctcggtttttggatctgcctcgggcttcactgatatgcttgtgcaacatattccttctcctaaagatgctgcaacaaggaaagtcgaccacacatatactggaccgaaagactctttgatttacaaagccatgaaaacttgtgatcccaatggcccgttgatggtaaatgttaccaagctgtatcccaagtccgattgtagcgtgttcgatgcatttggtagggtatacagtggcaagattcagacaggacagtcagtgcgagtattgggagaagggtattcacctgacgatgaggaggacatgacagtcaaagaagtaatgaagctatggctttaccaagcgcgagataggatacctatagctgaagctcctcctggtacttgggttctcatcgaaggggtggatgcttccataatgaaaactgccactctgtgtaatgagagttatgacgaggatgtatacagattctgtcctcttcagttcaatacactgccagtggtgaaaacggcaactgagcctctaaatcccagtgagttgccaaaaatggtggagggtcttagaaagatctgcaagagttatcctcttgccataaccaaagttgaggagtctggggagcacacaatcttaggtacaggagaactgtatttggattccatcatgaaggacctcagagagctctactctgaagtagaagtcaaggttgcagatcctgttgtttcattctgcgaaacagttgtggaatcttcatcaatgaagtgttttgctgaaacaccaaacaagaagaataaaattactatgattgcagagccattagagagaggacttgcggaggacattgagaaaggtgttgtaagcattgattggtctaggagagatataggtaatttcttccagactaggtatgagtgggatgttcttgctgcacggtccatatgggcattcggtcctgataagcagggacccaacatactacttggtgacacacattcaagcgaagttgacaagagcttgctgaatgctgtgaaaggctccattgtgcaagggtttcagtggggtgctcgagaaggacctctgtgtgatgaacccatcagaaatgttaagttcaagattgttgatgcaaggattgcacctgagccgttgcatcgtggatctggtcaaatcattcccacatctcgacgtgtggcctattcatctttcctaatggcaaccccacgggtcatggagccagtatattatgtggagatacaaacgccaatcgactgtgtatctgcaatctacactgttttatctcgcaggcgtggacatgttacagccgatgttcctcaacccgggaccccagcttacatcgttaaggcatttttacccgtcatagaatcgtttggttttgagacggacttgaggtaccatactcaaggacaggctttttgtctctcagtctttgatcattgggctatagttcctggggatcctcttgacaagagtattgttctgcgaccacttgaacccgcaccgattcagcactggctcgtgaatttatggtcaagacaaggcgtagaaagggaatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

902

Amino Acids

100.95

Weight (kDa)

5.16

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 32	6.5e-07	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
EFTUD2	PF16004	37 - 84	4e-13	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU_D2	PF03144	452 - 526	3.8e-09	Elongation factor Tu domain 2
EFG_IV	PF03764	672 - 783	1.6e-17	Elongation factor G, domain IV
EFG_C	PF00679	787 - 874	5.8e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	265, 1393, 1448
Acc65I	GGTACC	1 cut(s)	2544
AccB1I	GGYRCC	1 cut(s)	2544
AccI	GTMKAC	5 cut(s)	269, 395, 1227, 1368, 1602
AccII	CGCG	3 cut(s)	134, 883, 1486
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	5 cut(s)	122, 134, 883, 1970, 2649
AclWI	GGATC	9 cut(s)	767, 877, 893, 1162, 1280, 1844, 2299, 2603, 2616
AcsI	RAATTY	2 cut(s)	1017, 2674
AcuI	CTGAAG	3 cut(s)	1527, 1604, 1848
AdeI	CACNNNGTG	1 cut(s)	1575
AfaI	GTAC	8 cut(s)	146, 431, 506, 532, 762, 1522, 1774, 2546
AfiI	CCNNNNNNNGG	6 cut(s)	307, 733, 782, 964, 1303, 2471
AflIII	ACRYGT	2 cut(s)	2320, 2446
AjiI	CACGTC	1 cut(s)	2321
AjnI	CCWGG	5 cut(s)	60, 555, 1026, 1516, 2602
Alw21I	GWGCWC	3 cut(s)	1761, 1821, 2203
Alw26I	GTCTC	3 cut(s)	155, 2525, 2576
AlwI	GGATC	9 cut(s)	767, 877, 893, 1162, 1280, 1844, 2299, 2603, 2616
AlwNI	CAGNNNCTG	2 cut(s)	1611, 1853
Ama87I	CYCGRG	3 cut(s)	1162, 2202, 2471
Aor13HI	TCCGGA	1 cut(s)	97
AoxI	GGCC	3 cut(s)	1118, 1294, 2325
ApeKI	GCWGC	5 cut(s)	340, 737, 1097, 1211, 2063
ApoI	RAATTY	2 cut(s)	1017, 2674
ArsI	GACNNNNNNTTYG	2 cut(s)	2550, 2582
AseI	ATTAAT	1 cut(s)	720
Asp718I	GGTACC	1 cut(s)	2544
AspLEI	GCGC	3 cut(s)	325, 615, 1486
AspS9I	GGNCC	9 cut(s)	41, 1244, 1295, 1807, 2070, 2087, 2102, 2210, 2475
AsuC2I	CCSGG	2 cut(s)	2472, 2473
AsuHPI	GGTGA	6 cut(s)	161, 1042, 1075, 1416, 1654, 2132
AvaI	CYCGRG	3 cut(s)	1162, 2202, 2471
AvaII	GGWCC	8 cut(s)	41, 1244, 1807, 2070, 2087, 2102, 2210, 2475
BaeGI	GKGCMC	1 cut(s)	336
BaeI	ACNNNNGTAYC	2 cut(s)	1512, 1545
BamHI	GGATCC	1 cut(s)	2608
BanI	GGYRCC	1 cut(s)	2544
BanII	GRGCYC	1 cut(s)	1821
BauI	CACGAG	1 cut(s)	2669
BbsI	GAAGAC	1 cut(s)	356
Bbv12I	GWGCWC	3 cut(s)	1761, 1821, 2203
BbvI	GCAGC	5 cut(s)	352, 724, 1109, 1198, 2050
BccI	CCATC	4 cut(s)	810, 1297, 1805, 2237
BceAI	ACGGC	3 cut(s)	750, 1665, 2263
BcgI	CGANNNNNNTGC	2 cut(s)	801, 835
BciT130I	CCWGG	5 cut(s)	62, 557, 1028, 1518, 2604
BciVI	GTATCC	3 cut(s)	543, 1335, 1488
BclI	TGATCA	1 cut(s)	2584
BcnI	CCSGG	2 cut(s)	2472, 2473
BcoDI	GTCTC	3 cut(s)	155, 2525, 2576
BfaI	CTAG	2 cut(s)	2010, 2040
BfmI	CTRYAG	3 cut(s)	810, 1500, 2595
BfoI	RGCGCY	2 cut(s)	326, 616
BfuI	GTATCC	3 cut(s)	543, 1335, 1488
BglII	AGATCT	1 cut(s)	1707
BisI	GCNGC	6 cut(s)	123, 341, 738, 1098, 1212, 2064
BlsI	GCNGC	6 cut(s)	124, 342, 739, 1099, 1213, 2065
Bme1390I	CCNGG	7 cut(s)	62, 557, 1028, 1518, 2472, 2473, 2604
Bme18I	GGWCC	8 cut(s)	41, 1244, 1807, 2070, 2087, 2102, 2210, 2475
BmeT110I	CYCGRG	3 cut(s)	1162, 2202, 2471
BmgBI	CACGTC	1 cut(s)	2321
BmgT120I	GGNCC	9 cut(s)	41, 1244, 1295, 1807, 2070, 2087, 2102, 2210, 2475
BmiI	GGNNCC	9 cut(s)	43, 2103, 2104, 2173, 2367, 2476, 2477, 2546, 2610
BmrFI	CCNGG	7 cut(s)	62, 557, 1028, 1518, 2472, 2473, 2604
BmrI	ACTGGG	1 cut(s)	1665
BmsI	GCATC	9 cut(s)	134, 172, 394, 673, 1198, 1342, 1537, 2248, 2293
BmuI	ACTGGG	1 cut(s)	1665
BoxI	GACNNNNGTC	1 cut(s)	167
BpiI	GAAGAC	1 cut(s)	356
BplI	GAGNNNNNCTC	4 cut(s)	1708, 1740, 1808, 1840
Bpu10I	CCTNAGC	3 cut(s)	698, 1088, 2274
BpuEI	CTTGAG	3 cut(s)	529, 2538, 2560
BpuMI	CCSGG	2 cut(s)	2472, 2473
BsaJI	CCNNGG	4 cut(s)	1161, 2354, 2471, 2603
BsaWI	WCCGGW	1 cut(s)	97
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	6 cut(s)	307, 733, 782, 964, 1303, 2471
Bse1I	ACTGG	7 cut(s)	215, 991, 1246, 1637, 1671, 2369, 2669
Bse3DI	GCAATG	2 cut(s)	546, 1099
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	5 cut(s)	62, 557, 1028, 1518, 2604
BseDI	CCNNGG	4 cut(s)	1161, 2354, 2471, 2603
BseGI	GGATG	8 cut(s)	10, 181, 574, 821, 924, 1552, 1603, 2059
BseLI	CCNNNNNNNGG	6 cut(s)	307, 733, 782, 964, 1303, 2471
BseMI	GCAATG	2 cut(s)	546, 1099
BseMII	CTCAG	6 cut(s)	573, 1118, 1647, 1825, 2265, 2589
BseNI	ACTGG	7 cut(s)	215, 991, 1246, 1637, 1671, 2369, 2669
BseRI	GAGGAG	8 cut(s)	104, 152, 244, 483, 983, 1451, 1503, 1760
BseSI	GKGCMC	1 cut(s)	336
BseXI	GCAGC	5 cut(s)	352, 724, 1109, 1198, 2050
BseYI	CCCAGC	1 cut(s)	2479
BsgI	GTGCAG	1 cut(s)	2049
Bsh1236I	CGCG	3 cut(s)	134, 883, 1486
BshFI	GGCC	3 cut(s)	1120, 1296, 2327
BshNI	GGYRCC	1 cut(s)	2544
BsiHKAI	GWGCWC	3 cut(s)	1761, 1821, 2203
BsiHKCI	CYCGRG	3 cut(s)	1162, 2202, 2471
BsiSI	CCGG	2 cut(s)	98, 2472
BslFI	GGGAC	3 cut(s)	483, 2115, 2488
BslI	CCNNNNNNNGG	6 cut(s)	307, 733, 782, 964, 1303, 2471
BsmAI	GTCTC	3 cut(s)	155, 2525, 2576
BsmBI	CGTCTC	2 cut(s)	155, 2525
BsmFI	GGGAC	3 cut(s)	483, 2115, 2488
BsmI	GAATGC	2 cut(s)	2081, 2164
BsnI	GGCC	3 cut(s)	1120, 1296, 2327
BsoBI	CYCGRG	3 cut(s)	1162, 2202, 2471
Bsp1286I	GDGCHC	4 cut(s)	336, 1761, 1821, 2203
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	760
BspACI	CCGC	5 cut(s)	122, 134, 883, 1970, 2649
BspANI	GGCC	3 cut(s)	1120, 1296, 2327
BspCNI	CTCAG	6 cut(s)	574, 1119, 1648, 1824, 2266, 2588
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	3 cut(s)	134, 883, 1486
BspHI	TCATGA	1 cut(s)	1800
BspLI	GGNNCC	9 cut(s)	43, 2103, 2104, 2173, 2367, 2476, 2477, 2546, 2610
BspMAI	CTGCAG	1 cut(s)	814
BspPI	GGATC	9 cut(s)	767, 877, 893, 1162, 1280, 1844, 2299, 2603, 2616
BspT107I	GGYRCC	1 cut(s)	2544
BsrDI	GCAATG	2 cut(s)	546, 1099
BsrGI	TGTACA	1 cut(s)	760
BsrI	ACTGG	7 cut(s)	215, 991, 1246, 1637, 1671, 2369, 2669
BssECI	CCNNGG	4 cut(s)	1161, 2354, 2471, 2603
BssNAI	GTATAC	2 cut(s)	1369, 1603
BssSI	CACGAG	1 cut(s)	2669
Bst1107I	GTATAC	2 cut(s)	1369, 1603
Bst2BI	CACGAG	1 cut(s)	2669
Bst2UI	CCWGG	5 cut(s)	62, 557, 1028, 1518, 2604
Bst6I	CTCTTC	2 cut(s)	748, 1623
BstAPI	GCANNNNNTGC	2 cut(s)	1067, 2267
BstAUI	TGTACA	1 cut(s)	760
BstC8I	GCNNGC	2 cut(s)	2153, 2438
BstDSI	CCRYGG	1 cut(s)	2354
BstF5I	GGATG	8 cut(s)	10, 181, 574, 821, 924, 1552, 1603, 2059
BstFNI	CGCG	3 cut(s)	134, 883, 1486
BstH2I	RGCGCY	2 cut(s)	326, 616
BstHHI	GCGC	3 cut(s)	325, 615, 1486
BstMAI	GTCTC	3 cut(s)	155, 2525, 2576
BstMWI	GCNNNNNNNGC	6 cut(s)	131, 331, 340, 880, 1067, 2267
BstNI	CCWGG	5 cut(s)	62, 557, 1028, 1518, 2604
BstNSI	RCATGY	1 cut(s)	2450
BstPAI	GACNNNNGTC	1 cut(s)	167
BstSCI	CCNGG	7 cut(s)	60, 555, 1026, 1516, 2470, 2471, 2602
BstSFI	CTRYAG	3 cut(s)	810, 1500, 2595
BstSLI	GKGCMC	1 cut(s)	336
BstUI	CGCG	3 cut(s)	134, 883, 1486
BstV1I	GCAGC	5 cut(s)	352, 724, 1109, 1198, 2050
BstV2I	GAAGAC	1 cut(s)	356
BstX2I	RGATCY	5 cut(s)	1154, 1707, 1849, 2291, 2608
BstXI	CCANNNNNNTGG	1 cut(s)	1034
BstYI	RGATCY	5 cut(s)	1154, 1707, 1849, 2291, 2608
BstZ17I	GTATAC	2 cut(s)	1369, 1603
BsuI	GTATCC	3 cut(s)	543, 1335, 1488
BsuRI	GGCC	3 cut(s)	1120, 1296, 2327
BtgI	CCRYGG	1 cut(s)	2354
BtrI	CACGTC	1 cut(s)	2321
BtsCI	GGATG	8 cut(s)	10, 181, 574, 821, 924, 1552, 1603, 2059
BtsI	GCAGTG	1 cut(s)	1631
Cac8I	GCNNGC	2 cut(s)	2153, 2438
CaiI	CAGNNNCTG	2 cut(s)	1611, 1853
CciI	TCATGA	1 cut(s)	1800
CfoI	GCGC	3 cut(s)	325, 615, 1486
Cfr13I	GGNCC	9 cut(s)	41, 1244, 1295, 1807, 2070, 2087, 2102, 2210, 2475
Cfr9I	CCCGGG	1 cut(s)	2471
CseI	GACGC	3 cut(s)	140, 280, 889
Csp6I	GTAC	8 cut(s)	145, 430, 505, 531, 761, 1521, 1773, 2545
CspCI	CAANNNNNGTGG	2 cut(s)	1671, 1706
CviQI	GTAC	8 cut(s)	145, 430, 505, 531, 761, 1521, 1773, 2545
DraIII	CACNNNGTG	1 cut(s)	1575
DrdI	GACNNNNNNGTC	3 cut(s)	265, 1393, 1448
DseDI	GACNNNNNNGTC	3 cut(s)	265, 1393, 1448
Eam1104I	CTCTTC	2 cut(s)	748, 1623
EarI	CTCTTC	2 cut(s)	748, 1623
Ecl136II	GAGCTC	1 cut(s)	1819
Eco147I	AGGCCT	1 cut(s)	1120
Eco24I	GRGCYC	1 cut(s)	1821
Eco32I	GATATC	1 cut(s)	476
Eco47I	GGWCC	8 cut(s)	41, 1244, 1807, 2070, 2087, 2102, 2210, 2475
Eco53kI	GAGCTC	1 cut(s)	1819
Eco57I	CTGAAG	3 cut(s)	1527, 1604, 1848
Eco88I	CYCGRG	3 cut(s)	1162, 2202, 2471
EcoICRI	GAGCTC	1 cut(s)	1819
EcoO109I	RGGNCCY	5 cut(s)	41, 1807, 2102, 2210, 2475
EcoRII	CCWGG	5 cut(s)	60, 555, 1026, 1516, 2602
EcoRV	GATATC	1 cut(s)	476
EcoT22I	ATGCAT	1 cut(s)	1357
EcoT38I	GRGCYC	1 cut(s)	1821
Esp3I	CGTCTC	2 cut(s)	155, 2525
FalI	AAGNNNNNCTT	2 cut(s)	670, 702
FaqI	GGGAC	3 cut(s)	483, 2115, 2488
FauI	CCCGC	1 cut(s)	2656
FauNDI	CATATG	2 cut(s)	390, 2075
FbaI	TGATCA	1 cut(s)	2584
FblI	GTMKAC	5 cut(s)	269, 395, 1227, 1368, 1602
Fnu4HI	GCNGC	6 cut(s)	123, 341, 738, 1098, 1212, 2064
FokI	GGATG	8 cut(s)	17, 188, 581, 828, 911, 1559, 1610, 2066
FriOI	GRGCYC	1 cut(s)	1821
Fsp4HI	GCNGC	6 cut(s)	123, 341, 738, 1098, 1212, 2064
FspBI	CTAG	2 cut(s)	2010, 2040
GlaI	GCGC	3 cut(s)	324, 614, 1485
GluI	GCNGC	6 cut(s)	123, 341, 738, 1098, 1212, 2064
GsaI	CCCAGC	1 cut(s)	2483
HaeII	RGCGCY	2 cut(s)	326, 616
HaeIII	GGCC	3 cut(s)	1120, 1296, 2327
HapII	CCGG	2 cut(s)	98, 2472
HgaI	GACGC	3 cut(s)	140, 280, 889
HhaI	GCGC	3 cut(s)	325, 615, 1486
Hin6I	GCGC	3 cut(s)	323, 613, 1484
HinP1I	GCGC	3 cut(s)	323, 613, 1484
HincII	GTYRAC	4 cut(s)	270, 646, 1228, 2143
HindII	GTYRAC	4 cut(s)	270, 646, 1228, 2143
HindIII	AAGCTT	2 cut(s)	695, 859
HpaII	CCGG	2 cut(s)	98, 2472
HphI	GGTGA	6 cut(s)	161, 1042, 1075, 1416, 1654, 2132
Hpy188I	TCNGA	9 cut(s)	58, 130, 222, 444, 1336, 1386, 1814, 1828, 2234
Hpy99I	CGWCG	4 cut(s)	23, 262, 274, 2322
HpyAV	CCTTC	5 cut(s)	1206, 1409, 1532, 1798, 2201
HpyCH4IV	ACGT	5 cut(s)	164, 260, 789, 827, 2320
HpyF10VI	GCNNNNNNNGC	6 cut(s)	131, 331, 340, 880, 1067, 2267
HpySE526I	ACGT	5 cut(s)	164, 260, 789, 827, 2320
HspAI	GCGC	3 cut(s)	323, 613, 1484
KflI	GGGWCCC	2 cut(s)	2102, 2475
Kpn2I	TCCGGA	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	2548
Ksp22I	TGATCA	1 cut(s)	2584
LmnI	GCTCC	5 cut(s)	91, 1516, 1756, 2177, 2365
Lsp1109I	GCAGC	5 cut(s)	352, 724, 1109, 1198, 2050
LweI	GCATC	9 cut(s)	134, 172, 394, 673, 1198, 1342, 1537, 2248, 2293
MaeI	CTAG	2 cut(s)	2010, 2040
MaeII	ACGT	5 cut(s)	164, 260, 789, 827, 2320
MaeIII	GTNAC	4 cut(s)	1081, 1312, 2120, 2449
MfeI	CAATTG	1 cut(s)	621
MflI	RGATCY	5 cut(s)	1154, 1707, 1849, 2291, 2608
MhlI	GDGCHC	4 cut(s)	336, 1761, 1821, 2203
MluCI	AATT	8 cut(s)	519, 621, 668, 1017, 1106, 1932, 2026, 2674
MlyI	GAGTC	5 cut(s)	62, 245, 376, 1247, 1757
Mph1103I	ATGCAT	1 cut(s)	1357
MroI	TCCGGA	1 cut(s)	97
MseI	TTAA	7 cut(s)	228, 482, 666, 720, 857, 2241, 2493
MslI	CAYNNNNRTG	1 cut(s)	1175
MspA1I	CMGCKG	1 cut(s)	202
MspI	CCGG	2 cut(s)	98, 2472
MspR9I	CCNGG	7 cut(s)	62, 557, 1028, 1518, 2472, 2473, 2604
MunI	CAATTG	1 cut(s)	621
Mva1269I	GAATGC	2 cut(s)	2081, 2164
MvaI	CCWGG	5 cut(s)	62, 557, 1028, 1518, 2604
MvnI	CGCG	3 cut(s)	134, 883, 1486
MwoI	GCNNNNNNNGC	6 cut(s)	131, 331, 340, 880, 1067, 2267
NciI	CCSGG	2 cut(s)	2472, 2473
NdeI	CATATG	2 cut(s)	390, 2075
NlaIV	GGNNCC	9 cut(s)	43, 2103, 2104, 2173, 2367, 2476, 2477, 2546, 2610
NmuCI	GTSAC	2 cut(s)	1081, 2120
NsiI	ATGCAT	1 cut(s)	1357
NspI	RCATGY	1 cut(s)	2450
PaeR7I	CTCGAG	1 cut(s)	2202
PagI	TCATGA	1 cut(s)	1800
PceI	AGGCCT	1 cut(s)	1120
PciI	ACATGT	1 cut(s)	2446
PcsI	WCGNNNNNNNCGW	1 cut(s)	266
PctI	GAATGC	2 cut(s)	2081, 2164
PfeI	GAWTC	6 cut(s)	1381, 1608, 1793, 1883, 2516, 2656
PkrI	GCNGC	6 cut(s)	124, 342, 739, 1099, 1213, 2065
PleI	GAGTC	5 cut(s)	61, 245, 376, 1247, 1756
PpsI	GAGTC	5 cut(s)	61, 245, 376, 1247, 1756
PpuMI	RGGWCCY	5 cut(s)	41, 1807, 2102, 2210, 2475
PscI	ACATGT	1 cut(s)	2446
PshAI	GACNNNNGTC	1 cut(s)	167
PshBI	ATTAAT	1 cut(s)	720
Psp124BI	GAGCTC	1 cut(s)	1821
Psp5II	RGGWCCY	5 cut(s)	41, 1807, 2102, 2210, 2475
Psp6I	CCWGG	5 cut(s)	60, 555, 1026, 1516, 2602
PspFI	CCCAGC	1 cut(s)	2479
PspGI	CCWGG	5 cut(s)	60, 555, 1026, 1516, 2602
PspN4I	GGNNCC	9 cut(s)	43, 2103, 2104, 2173, 2367, 2476, 2477, 2546, 2610
PspPI	GGNCC	9 cut(s)	41, 1244, 1295, 1807, 2070, 2087, 2102, 2210, 2475
PspPPI	RGGWCCY	5 cut(s)	41, 1807, 2102, 2210, 2475
PstI	CTGCAG	1 cut(s)	814
PstNI	CAGNNNCTG	2 cut(s)	1611, 1853
PsuI	RGATCY	5 cut(s)	1154, 1707, 1849, 2291, 2608
PvuII	CAGCTG	1 cut(s)	202
RsaI	GTAC	8 cut(s)	146, 431, 506, 532, 762, 1522, 1774, 2546
RsaNI	GTAC	8 cut(s)	145, 430, 505, 531, 761, 1521, 1773, 2545
RseI	CAYNNNNRTG	1 cut(s)	1175
SacI	GAGCTC	1 cut(s)	1821
SalI	GTCGAC	2 cut(s)	268, 1226
SaqAI	TTAA	7 cut(s)	228, 482, 666, 720, 857, 2241, 2493
SatI	GCNGC	6 cut(s)	123, 341, 738, 1098, 1212, 2064
Sau96I	GGNCC	9 cut(s)	41, 1244, 1295, 1807, 2070, 2087, 2102, 2210, 2475
SchI	GAGTC	5 cut(s)	62, 245, 376, 1247, 1757
ScrFI	CCNGG	7 cut(s)	62, 557, 1028, 1518, 2472, 2473, 2604
SduI	GDGCHC	4 cut(s)	336, 1761, 1821, 2203
SfaNI	GCATC	9 cut(s)	134, 172, 394, 673, 1198, 1342, 1537, 2248, 2293
SfcI	CTRYAG	3 cut(s)	810, 1500, 2595
Sfr274I	CTCGAG	1 cut(s)	2202
SinI	GGWCC	8 cut(s)	41, 1244, 1807, 2070, 2087, 2102, 2210, 2475
SlaI	CTCGAG	1 cut(s)	2202
SmaI	CCCGGG	1 cut(s)	2473
SmiMI	CAYNNNNRTG	1 cut(s)	1175
SmlI	CTYRAG	4 cut(s)	508, 2202, 2539, 2553
SmoI	CTYRAG	4 cut(s)	508, 2202, 2539, 2553
Sse9I	AATT	8 cut(s)	519, 621, 668, 1017, 1106, 1932, 2026, 2674
SseBI	AGGCCT	1 cut(s)	1120
SsiI	CCGC	5 cut(s)	122, 134, 883, 1970, 2649
SspI	AATATT	1 cut(s)	226
SspMI	CTAG	2 cut(s)	2010, 2040
SstI	GAGCTC	1 cut(s)	1821
StuI	AGGCCT	1 cut(s)	1120
StyD4I	CCNGG	7 cut(s)	60, 555, 1026, 1516, 2470, 2471, 2602
TaiI	ACGT	5 cut(s)	167, 263, 792, 830, 2323
TaqII	GACCGA	2 cut(s)	1261, 2075
TasI	AATT	8 cut(s)	519, 621, 668, 1017, 1106, 1932, 2026, 2674
TatI	WGTACW	2 cut(s)	504, 760
TauI	GCSGC	1 cut(s)	125
TfiI	GAWTC	6 cut(s)	1381, 1608, 1793, 1883, 2516, 2656
Tru1I	TTAA	7 cut(s)	228, 482, 666, 720, 857, 2241, 2493
Tru9I	TTAA	7 cut(s)	228, 482, 666, 720, 857, 2241, 2493
TseFI	GTSAC	2 cut(s)	1081, 2120
TseI	GCWGC	5 cut(s)	340, 737, 1097, 1211, 2063
Tsp45I	GTSAC	2 cut(s)	1081, 2120
TspGWI	ACGGA	1 cut(s)	2549
TspMI	CCCGGG	1 cut(s)	2471
VpaK11BI	GGWCC	8 cut(s)	41, 1244, 1807, 2070, 2087, 2102, 2210, 2475
VspI	ATTAAT	1 cut(s)	720
XapI	RAATTY	2 cut(s)	1017, 2674
XceI	RCATGY	1 cut(s)	2450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1238
XhoI	CTCGAG	1 cut(s)	2202
XmaI	CCCGGG	1 cut(s)	2471
XmiI	GTMKAC	5 cut(s)	269, 395, 1227, 1368, 1602
XspI	CTAG	2 cut(s)	2010, 2040
Zsp2I	ATGCAT	1 cut(s)	1357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.