Rmu_sc0001460.1_g000011

Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001460.1
OG0004109
Physical Location & Seq
Forward (+)
58633 .. 60906
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
atggatgggatgggtgcatctggaaaccacgacctaggaggtcacagcatcattaatgactttgagttacaagacttcatagatgatgcaaacttcgaccagttcatcaatcttattcgcggcgaaaatgaggatccagcagcagctcattttgattgcgaccttatcagtggctcgtgctttgttgataaccagtttaatccaactactccgggtagtggtttctttggtttagatcataatactactgtcggatccgatccagctccttttcttactacattaccaaattttgatggagaaatgaacgatattggggaagaagataatgacgcagactcatgtggaacaacgacaacaaccaccacaaataccacagccacgacgacaaagaagccaaaggttgatcggtccagaaccttggtttctgagaggaagaggaggggaaggatgaaagaaaaactctatgcattgcggtccttggttcctaacatcactaagatggataaagcctccatcgttggagatgctgtatcatatgtacaagatcttcaaaaacacgctaagaagctggaagctgagatttcaggtcttgaagcatccatagaaggatcaggggtaaagcaagcagtatcaacccaaaatccaaccaagaataaattagttgcagacaagtaccatttaactcccacggaaatcattcagatagatgtatcccaagtagaggagaaaagcttttatgtgaaagtgggatgcaacaaaggagtaggcgtggccacagcgctttacaaggctatcgaatcccttacaagcttcaatgttctgagttcaaacttgaacacggttaattctgggagattcgaaataacactcagtttgaatgtgaaagaaagcgagcaggagatcatcaacttaccaaacctgaaactttgggagagcaccaacctcgtcacaaggttcttttctctgcctttcggaaaaggacttctttccttacgggtaagggctttggttgtgatctcttcgcgtcaccgaatagatactcaacattgtaggttgagcagaacaatcactgggaagtttggagaaagcatgggtttaataaagcgtatctttagcttagctgggttgcgagggcattacccttacttcgctgggttgcgagggcgttacccttactgcgctagtttgcaactgggttgcaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

402

Amino Acids

44.17

Weight (kDa)

6.52

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 141 - 185 5.2e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1194
AccII CGCG 2 cut(s) 120, 1027
AciI CCGC 2 cut(s) 120, 475
AclWI GGATC 6 cut(s) 128, 141, 249, 254, 262, 619
AcoI YGGCCR 1 cut(s) 774
AcsI RAATTY 1 cut(s) 289
AfaI GTAC 2 cut(s) 543, 677
AfeI AGCGCT 1 cut(s) 783
AfiI CCNNNNNNNGG 2 cut(s) 218, 724
AgsI TTSAA 6 cut(s) 554, 596, 817, 831, 838, 880
AluBI AGCT 8 cut(s) 146, 266, 571, 578, 735, 813, 1119, 1124
AluI AGCT 8 cut(s) 146, 266, 571, 578, 735, 813, 1119, 1124
Alw21I GWGCWC 1 cut(s) 941
AlwI GGATC 6 cut(s) 128, 141, 249, 254, 262, 619
Aor51HI AGCGCT 1 cut(s) 783
AoxI GGCC 1 cut(s) 774
ApeKI GCWGC 2 cut(s) 140, 143
ApoI RAATTY 1 cut(s) 289
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 699
AspA2I CCTAGG 1 cut(s) 34
AspLEI GCGC 2 cut(s) 784, 1184
AspS9I GGNCC 2 cut(s) 411, 477
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 1022
AsuII TTCGAA 1 cut(s) 861
AvaII GGWCC 2 cut(s) 411, 477
AvrII CCTAGG 1 cut(s) 34
BalI TGGCCA 1 cut(s) 776
BamHI GGATCC 2 cut(s) 133, 254
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BauI CACGAG 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 941
BbvI GCAGC 2 cut(s) 152, 155
BccI CCATC 4 cut(s) 4, 290, 496, 524
BcgI CGANNNNNNTGC 2 cut(s) 928, 962
BciVI GTATCC 1 cut(s) 724
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 2 cut(s) 35, 1185
BfoI RGCGCY 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 1194
BfuI GTATCC 1 cut(s) 724
BglII AGATCT 1 cut(s) 547
BisI GCNGC 3 cut(s) 121, 141, 144
BlnI CCTAGG 1 cut(s) 34
BlpI GCTNAGC 1 cut(s) 1120
BlsI GCNGC 3 cut(s) 122, 142, 145
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 411, 477
BmgT120I GGNCC 2 cut(s) 411, 477
BmiI GGNNCC 3 cut(s) 135, 256, 486
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 2 cut(s) 1083, 1205
BmsI GCATC 6 cut(s) 26, 57, 76, 517, 608, 743
BmuI ACTGGG 2 cut(s) 1083, 1205
Bpu1102I GCTNAGC 1 cut(s) 1120
Bpu14I TTCGAA 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 34, 420, 480, 690
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 756, 786
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 724
Bse1I ACTGG 4 cut(s) 100, 193, 1078, 1200
Bse3DI GCAATG 1 cut(s) 470
BseDI CCNNGG 4 cut(s) 34, 420, 480, 690
BseGI GGATG 5 cut(s) 10, 15, 456, 599, 758
BseLI CCNNNNNNNGG 2 cut(s) 218, 724
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 4 cut(s) 420, 570, 815, 886
BseNI ACTGG 4 cut(s) 100, 193, 1078, 1200
BseRI GAGGAG 2 cut(s) 454, 740
BseXI GCAGC 2 cut(s) 152, 155
BseYI CCCAGC 2 cut(s) 1124, 1154
Bsh1236I CGCG 2 cut(s) 120, 1027
BshFI GGCC 1 cut(s) 776
BsiHKAI GWGCWC 1 cut(s) 941
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 218, 724
BsnI GGCC 1 cut(s) 776
Bsp119I TTCGAA 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 941
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
Bsp1720I GCTNAGC 1 cut(s) 1120
BspACI CCGC 2 cut(s) 120, 475
BspANI GGCC 1 cut(s) 776
BspCNI CTCAG 4 cut(s) 421, 571, 816, 885
BspFNI CGCG 2 cut(s) 120, 1027
BspLI GGNNCC 3 cut(s) 135, 256, 486
BspMI ACCTGC 1 cut(s) 1194
BspPI GGATC 6 cut(s) 128, 141, 249, 254, 262, 619
BspT104I TTCGAA 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 4 cut(s) 100, 193, 1078, 1200
BssECI CCNNGG 4 cut(s) 34, 420, 480, 690
BssMI GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
BssSI CACGAG 1 cut(s) 175
BssT1I CCWWGG 3 cut(s) 34, 420, 480
Bst2BI CACGAG 1 cut(s) 175
Bst4CI ACNGT 2 cut(s) 250, 844
Bst6I CTCTTC 2 cut(s) 431, 1027
BstAUI TGTACA 1 cut(s) 541
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 2 cut(s) 627, 896
BstDEI CTNAG 7 cut(s) 429, 498, 564, 579, 824, 872, 1120
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 5 cut(s) 10, 15, 456, 599, 758
BstFNI CGCG 2 cut(s) 120, 1027
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 2 cut(s) 784, 1184
BstKTI GATC 9 cut(s) 136, 238, 257, 262, 409, 550, 614, 906, 1020
BstMBI GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
BstSCI CCNGG 1 cut(s) 211
BstUI CGCG 2 cut(s) 120, 1027
BstV1I GCAGC 2 cut(s) 152, 155
BstX2I RGATCY 3 cut(s) 133, 254, 547
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 3 cut(s) 133, 254, 547
BsuI GTATCC 1 cut(s) 724
BsuRI GGCC 1 cut(s) 776
BtgI CCRYGG 1 cut(s) 690
BtsCI GGATG 5 cut(s) 10, 15, 456, 599, 758
BtsIMutI CAGTG 2 cut(s) 175, 1071
BveI ACCTGC 1 cut(s) 1194
Cac8I GCNNGC 2 cut(s) 627, 896
CfoI GCGC 2 cut(s) 784, 1184
Cfr13I GGNCC 2 cut(s) 411, 477
CseI GACGC 2 cut(s) 341, 1016
Csp6I GTAC 2 cut(s) 542, 676
CviAII CATG 2 cut(s) 342, 1093
CviQI GTAC 2 cut(s) 542, 676
DdeI CTNAG 7 cut(s) 429, 498, 564, 579, 824, 872, 1120
DpnI GATC 9 cut(s) 135, 237, 256, 261, 408, 549, 613, 905, 1019
DpnII GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
EaeI YGGCCR 1 cut(s) 774
Eam1104I CTCTTC 2 cut(s) 431, 1027
EarI CTCTTC 2 cut(s) 431, 1027
Eco130I CCWWGG 3 cut(s) 34, 420, 480
Eco47I GGWCC 2 cut(s) 411, 477
Eco47III AGCGCT 1 cut(s) 783
EcoT14I CCWWGG 3 cut(s) 34, 420, 480
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 3 cut(s) 34, 420, 480
FaeI CATG 2 cut(s) 345, 1096
FaiI YATR 9 cut(s) 80, 240, 343, 468, 538, 540, 605, 741, 1094
FalI AAGNNNNNCTT 4 cut(s) 972, 1004, 1097, 1129
FatI CATG 2 cut(s) 341, 1092
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 121, 141, 144
FokI GGATG 5 cut(s) 17, 22, 463, 586, 765
Fsp4HI GCNGC 3 cut(s) 121, 141, 144
FspBI CTAG 2 cut(s) 35, 1185
GlaI GCGC 2 cut(s) 783, 1183
GluI GCNGC 3 cut(s) 121, 141, 144
GsaI CCCAGC 2 cut(s) 1128, 1158
HaeII RGCGCY 1 cut(s) 785
HaeIII GGCC 1 cut(s) 776
HapII CCGG 1 cut(s) 212
HgaI GACGC 2 cut(s) 341, 1016
HhaI GCGC 2 cut(s) 784, 1184
Hin1II CATG 2 cut(s) 345, 1096
Hin6I GCGC 2 cut(s) 782, 1182
HinP1I GCGC 2 cut(s) 782, 1182
HindIII AAGCTT 2 cut(s) 733, 811
HinfI GANTC 3 cut(s) 338, 800, 858
HpaII CCGG 1 cut(s) 212
HphI GGTGA 1 cut(s) 1022
Hpy188I TCNGA 6 cut(s) 254, 259, 430, 705, 825, 977
Hpy188III TCNNGA 3 cut(s) 21, 414, 593
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 441, 602
HpyCH4III ACNGT 2 cut(s) 250, 844
HpyCH4V TGCA 7 cut(s) 17, 89, 470, 668, 756, 1192, 1203
HpyF3I CTNAG 7 cut(s) 429, 498, 564, 579, 824, 872, 1120
Hsp92II CATG 2 cut(s) 345, 1096
HspAI GCGC 2 cut(s) 782, 1182
Kzo9I GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
LmnI GCTCC 1 cut(s) 271
Lsp1109I GCAGC 2 cut(s) 152, 155
LweI GCATC 6 cut(s) 26, 57, 76, 517, 608, 743
MaeI CTAG 2 cut(s) 35, 1185
MaeIII GTNAC 5 cut(s) 41, 66, 949, 1028, 1169
MalI GATC 9 cut(s) 135, 237, 256, 261, 408, 549, 613, 905, 1019
MboI GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
MboII GAAGA 5 cut(s) 332, 335, 448, 542, 1014
MflI RGATCY 3 cut(s) 133, 254, 547
MhlI GDGCHC 1 cut(s) 941
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 3 cut(s) 289, 659, 847
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 4 cut(s) 227, 232, 502, 671
Mox20I TGGCCA 1 cut(s) 776
Mph1103I ATGCAT 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 699
MscI TGGCCA 1 cut(s) 776
MseI TTAA 5 cut(s) 54, 198, 683, 846, 1100
MslI CAYNNNNRTG 1 cut(s) 500
Msp20I TGGCCA 1 cut(s) 776
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 213
MvnI CGCG 2 cut(s) 120, 1027
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 538
NdeII GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
NlaIII CATG 2 cut(s) 345, 1096
NlaIV GGNNCC 3 cut(s) 135, 256, 486
NmuCI GTSAC 3 cut(s) 41, 949, 1028
NsiI ATGCAT 1 cut(s) 472
NspV TTCGAA 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 2 cut(s) 800, 858
PkrI GCNGC 3 cut(s) 122, 142, 145
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PshBI ATTAAT 1 cut(s) 54
PspFI CCCAGC 2 cut(s) 1124, 1154
PspN4I GGNNCC 3 cut(s) 135, 256, 486
PspPI GGNCC 2 cut(s) 411, 477
PsuI RGATCY 3 cut(s) 133, 254, 547
RsaI GTAC 2 cut(s) 543, 677
RsaNI GTAC 2 cut(s) 542, 676
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 5 cut(s) 54, 198, 683, 846, 1100
SatI GCNGC 3 cut(s) 121, 141, 144
Sau3AI GATC 9 cut(s) 133, 235, 254, 259, 406, 547, 611, 903, 1017
Sau96I GGNCC 2 cut(s) 411, 477
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 941
SfaNI GCATC 6 cut(s) 26, 57, 76, 517, 608, 743
SfuI TTCGAA 1 cut(s) 861
SinI GGWCC 2 cut(s) 411, 477
SmiMI CAYNNNNRTG 1 cut(s) 500
Sse9I AATT 3 cut(s) 289, 659, 847
SsiI CCGC 2 cut(s) 120, 475
SspMI CTAG 2 cut(s) 35, 1185
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 3 cut(s) 34, 420, 480
TaaI ACNGT 2 cut(s) 250, 844
TaqI TCGA 3 cut(s) 96, 798, 861
TaqII GACCGA 1 cut(s) 399
TasI AATT 3 cut(s) 289, 659, 847
TatI WGTACW 1 cut(s) 541
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 2 cut(s) 800, 858
Tru1I TTAA 5 cut(s) 54, 198, 683, 846, 1100
Tru9I TTAA 5 cut(s) 54, 198, 683, 846, 1100
TscAI CASTG 2 cut(s) 175, 1078
TseFI GTSAC 3 cut(s) 41, 949, 1028
TseI GCWGC 2 cut(s) 140, 143
Tsp45I GTSAC 3 cut(s) 41, 949, 1028
TspDTI ATGAA 4 cut(s) 67, 94, 320, 467
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 2 cut(s) 175, 1078
VpaK11BI GGWCC 2 cut(s) 411, 477
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 289
XmaJI CCTAGG 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 2 cut(s) 35, 1185
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.