Rmu_sc0001878.1_g000009

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001878.1
OG0006424
Physical Location & Seq
Reverse (-)
37864 .. 42041
4178 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
atgtctgcaaatccgacgccgggttcgggtttgaaaatgggttgggtttgccggaatcgagggagagggcatttactcgattccttcatagacgacgacgctctggtcttgatctccgttcgctgcaagagactcacgcgcctcaagctccgcggctgtcgggaaatcacggacctcggaatggcggcgttcgctcagaattctactggattgaagaagctctccgtcgggtcgtgcttgttcggcaccaaggccatgaacgccgtgcttgagtactgcacggcgctcgaggagctatcggtgaagcggctccgggggatccacgacgcgtctgcaatgggtctcgggcctcaaccattgaagtcggagaagggagatccgcaaccgtcgaagtcaatgatgggaggagaagaagaaccggattccggtcatcggtcaccggatttcggttaccggtttccggaatccggacatcggtcgccggaatccggtcacctgtcgccggagaccgcgccggccactggtcaccggagcacagcaaggtggaagaagagtgtcgaacagaagttgaaattggcaattgaggaggagaagaaagcactggaggatacgcaacctaatctcctcggtgaggccaaggagacggaggaggccatcagcaacgcgtcatcaaattgtcatcacgaaacttgctctgaattgcgaccgccgatccaccaacatcggcgaccatcaatagcgtcggatctgcgccttcaccaagtatcacctccgttgacgtcatcgtcatccttgtctcgtcgccggccatctagaccagagacaagagctgaaggtcgtgcactggtgaagaacaacgcgtcgcagacagagatcaagtcggcgtataggagcttggtgaagctgcaccacccgaatgcctcgtcggaggccgaatcggaaggccaggatttcgtcgaaattctagagtcaatctaa

Protein Analysis

325

Amino Acids

35.61

Weight (kDa)

8.63

Isoelectric Point (pI)

66.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 283 - 323 1e-06 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 104, 784
AatII GACGTC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
AccIII TCCGGA 2 cut(s) 460, 467
AciI CCGC 7 cut(s) 151, 153, 185, 307, 380, 510, 707
AclWI GGATC 5 cut(s) 313, 326, 371, 706, 753
AcoI YGGCCR 2 cut(s) 516, 806
AcsI RAATTY 2 cut(s) 199, 960
AcuI CTGAAG 1 cut(s) 852
AcyI GRCGYC 2 cut(s) 17, 779
AfaI GTAC 1 cut(s) 275
AflIII ACRYGT 3 cut(s) 327, 663, 858
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 4 cut(s) 34, 214, 361, 571
AjnI CCWGG 1 cut(s) 945
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 6 cut(s) 148, 220, 295, 830, 894, 904
AluI AGCT 6 cut(s) 148, 220, 295, 830, 894, 904
Alw21I GWGCWC 2 cut(s) 536, 844
Alw26I GTCTC 6 cut(s) 124, 347, 500, 635, 801, 815
Alw44I GTGCAC 1 cut(s) 840
AlwI GGATC 5 cut(s) 313, 326, 371, 706, 753
Ama87I CYCGRG 2 cut(s) 287, 344
Aor13HI TCCGGA 2 cut(s) 460, 467
AoxI GGCC 8 cut(s) 252, 347, 516, 633, 651, 806, 930, 943
ApaLI GTGCAC 1 cut(s) 840
ApeKI GCWGC 2 cut(s) 123, 904
ApoI RAATTY 2 cut(s) 199, 960
ArsI GACNNNNNNTTYG 4 cut(s) 7, 39, 908, 940
AsiGI ACCGGT 1 cut(s) 453
AspLEI GCGC 4 cut(s) 141, 286, 514, 753
AspS9I GGNCC 2 cut(s) 172, 347
AsuC2I CCSGG 2 cut(s) 21, 314
AsuHPI GGTGA 9 cut(s) 313, 429, 485, 518, 641, 749, 759, 859, 910
AvaI CYCGRG 2 cut(s) 287, 344
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 844
BamHI GGATCC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 536, 844
BbvI GCAGC 2 cut(s) 110, 891
BccI CCATC 4 cut(s) 394, 662, 739, 817
BceAI ACGGC 2 cut(s) 248, 297
BcgI CGANNNNNNTGC 4 cut(s) 268, 302, 314, 348
BciT130I CCWGG 1 cut(s) 947
BciVI GTATCC 1 cut(s) 601
BcnI CCSGG 2 cut(s) 21, 314
BcoDI GTCTC 6 cut(s) 124, 347, 500, 635, 801, 815
BfaI CTAG 2 cut(s) 813, 965
BfoI RGCGCY 1 cut(s) 287
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 5 cut(s) 124, 154, 186, 308, 905
BlsI GCNGC 5 cut(s) 125, 155, 187, 309, 906
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 3 cut(s) 21, 314, 947
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 2 cut(s) 287, 344
BmgT120I GGNCC 2 cut(s) 172, 347
BmiI GGNNCC 3 cut(s) 247, 311, 320
BmrFI CCNGG 3 cut(s) 21, 314, 947
BoxI GACNNNNGTC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 128, 290
BpuMI CCSGG 2 cut(s) 21, 314
BsaHI GRCGYC 2 cut(s) 17, 779
BsaI GGTCTC 2 cut(s) 347, 500
BsaJI CCNNGG 6 cut(s) 151, 175, 249, 313, 625, 636
BsaWI WCCGGW 8 cut(s) 418, 425, 439, 453, 460, 467, 488, 528
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bse118I RCCGGY 3 cut(s) 453, 514, 804
Bse1I ACTGG 4 cut(s) 211, 526, 606, 849
Bse3DI GCAATG 1 cut(s) 342
BseAI TCCGGA 2 cut(s) 460, 467
BseBI CCWGG 1 cut(s) 947
BseDI CCNNGG 6 cut(s) 151, 175, 249, 313, 625, 636
BseGI GGATG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 4 cut(s) 211, 526, 606, 849
BseRI GAGGAG 6 cut(s) 305, 420, 599, 602, 614, 662
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 2 cut(s) 110, 891
BsgI GTGCAG 2 cut(s) 262, 890
Bsh1236I CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
Bsh1285I CGRYCG 2 cut(s) 479, 707
BshFI GGCC 8 cut(s) 254, 349, 518, 635, 653, 808, 932, 945
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 453
BsiEI CGRYCG 2 cut(s) 479, 707
BsiHKAI GWGCWC 2 cut(s) 536, 844
BsiHKCI CYCGRG 2 cut(s) 287, 344
BsmAI GTCTC 6 cut(s) 124, 347, 500, 635, 801, 815
BsmBI CGTCTC 1 cut(s) 635
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 8 cut(s) 254, 349, 518, 635, 653, 808, 932, 945
Bso31I GGTCTC 2 cut(s) 347, 500
BsoBI CYCGRG 2 cut(s) 287, 344
Bsp1286I GDGCHC 2 cut(s) 536, 844
Bsp13I TCCGGA 2 cut(s) 460, 467
Bsp143I GATC 6 cut(s) 111, 318, 376, 711, 745, 873
BspACI CCGC 7 cut(s) 151, 153, 185, 307, 380, 510, 707
BspANI GGCC 8 cut(s) 254, 349, 518, 635, 653, 808, 932, 945
BspCNI CTCAG 1 cut(s) 208
BspEI TCCGGA 2 cut(s) 460, 467
BspFNI CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
BspLI GGNNCC 3 cut(s) 247, 311, 320
BspPI GGATC 5 cut(s) 313, 326, 371, 706, 753
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 2 cut(s) 347, 500
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 3 cut(s) 453, 514, 804
BsrI ACTGG 4 cut(s) 211, 526, 606, 849
BssAI RCCGGY 3 cut(s) 453, 514, 804
BssECI CCNNGG 6 cut(s) 151, 175, 249, 313, 625, 636
BssMI GATC 6 cut(s) 111, 318, 376, 711, 745, 873
BssNI GRCGYC 2 cut(s) 17, 779
BssT1I CCWWGG 2 cut(s) 249, 636
Bst2UI CCWGG 1 cut(s) 947
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 545
BstACI GRCGYC 2 cut(s) 17, 779
BstC8I GCNNGC 2 cut(s) 516, 806
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 151
BstEII GGTNACC 4 cut(s) 435, 449, 491, 524
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 1 cut(s) 788
BstFNI CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 4 cut(s) 141, 286, 514, 753
BstKTI GATC 6 cut(s) 114, 321, 379, 714, 748, 876
BstMAI GTCTC 6 cut(s) 124, 347, 500, 635, 801, 815
BstMBI GATC 6 cut(s) 111, 318, 376, 711, 745, 873
BstMCI CGRYCG 2 cut(s) 479, 707
BstMWI GCNNNNNNNGC 5 cut(s) 145, 191, 243, 260, 292
BstNI CCWGG 1 cut(s) 947
BstPAI GACNNNNGTC 1 cut(s) 475
BstPI GGTNACC 4 cut(s) 435, 449, 491, 524
BstSCI CCNGG 3 cut(s) 19, 312, 945
BstSLI GKGCMC 1 cut(s) 844
BstUI CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
BstV1I GCAGC 2 cut(s) 110, 891
BstX2I RGATCY 3 cut(s) 318, 376, 745
BstYI RGATCY 3 cut(s) 318, 376, 745
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 8 cut(s) 254, 349, 518, 635, 653, 808, 932, 945
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 788
BtsIMutI CAGTG 3 cut(s) 519, 599, 842
Cac8I GCNNGC 2 cut(s) 516, 806
CfoI GCGC 4 cut(s) 141, 286, 514, 753
Cfr10I RCCGGY 3 cut(s) 453, 514, 804
Cfr13I GGNCC 2 cut(s) 172, 347
Cfr42I CCGCGG 1 cut(s) 154
CseI GACGC 7 cut(s) 25, 107, 318, 335, 654, 729, 849
Csp6I GTAC 1 cut(s) 274
CspAI ACCGGT 1 cut(s) 453
CviAII CATG 1 cut(s) 256
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 195
DpnI GATC 6 cut(s) 113, 320, 378, 713, 747, 875
DpnII GATC 6 cut(s) 111, 318, 376, 711, 745, 873
DrdI GACNNNNNNGTC 2 cut(s) 104, 784
DseDI GACNNNNNNGTC 2 cut(s) 104, 784
EaeI YGGCCR 2 cut(s) 516, 806
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco130I CCWWGG 2 cut(s) 249, 636
Eco31I GGTCTC 2 cut(s) 347, 500
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 852
Eco88I CYCGRG 2 cut(s) 287, 344
Eco91I GGTNACC 4 cut(s) 435, 449, 491, 524
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoO65I GGTNACC 4 cut(s) 435, 449, 491, 524
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 945
EcoT14I CCWWGG 2 cut(s) 249, 636
ErhI CCWWGG 2 cut(s) 249, 636
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 1 cut(s) 259
FaiI YATR 3 cut(s) 89, 257, 888
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 5 cut(s) 124, 154, 186, 308, 905
FokI GGATG 1 cut(s) 775
Fsp4HI GCNGC 5 cut(s) 124, 154, 186, 308, 905
FspBI CTAG 2 cut(s) 813, 965
GlaI GCGC 4 cut(s) 140, 285, 513, 752
GluI GCNGC 5 cut(s) 124, 154, 186, 308, 905
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 8 cut(s) 254, 349, 518, 635, 653, 808, 932, 945
HgaI GACGC 7 cut(s) 25, 107, 318, 335, 654, 729, 849
HhaI GCGC 4 cut(s) 141, 286, 514, 753
Hin1I GRCGYC 2 cut(s) 17, 779
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 4 cut(s) 139, 284, 512, 751
HinP1I GCGC 4 cut(s) 139, 284, 512, 751
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 8 cut(s) 55, 80, 132, 422, 464, 485, 935, 968
HphI GGTGA 9 cut(s) 313, 429, 485, 518, 641, 749, 759, 859, 910
Hpy166II GTNNAC 2 cut(s) 777, 842
Hpy188I TCNGA 8 cut(s) 15, 179, 198, 367, 697, 745, 928, 940
Hpy188III TCNNGA 7 cut(s) 109, 161, 461, 468, 683, 813, 965
Hpy8I GTNNAC 2 cut(s) 777, 842
HpyAV CCTTC 5 cut(s) 94, 364, 764, 827, 935
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 779
HpyCH4V TGCA 6 cut(s) 8, 126, 279, 335, 842, 907
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 191, 243, 260, 292
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 779
Hsp92I GRCGYC 2 cut(s) 17, 779
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 4 cut(s) 139, 284, 512, 751
Kpn2I TCCGGA 2 cut(s) 460, 467
KroI GCCGGC 2 cut(s) 514, 804
KroNI GCCGGC 2 cut(s) 516, 806
KspI CCGCGG 1 cut(s) 154
Kzo9I GATC 6 cut(s) 111, 318, 376, 711, 745, 873
LmnI GCTCC 5 cut(s) 153, 292, 315, 531, 891
Lsp1109I GCAGC 2 cut(s) 110, 891
MaeI CTAG 2 cut(s) 813, 965
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 4 cut(s) 435, 449, 491, 524
MalI GATC 6 cut(s) 113, 320, 378, 713, 747, 875
MboI GATC 6 cut(s) 111, 318, 376, 711, 745, 873
MboII GAAGA 7 cut(s) 226, 422, 425, 559, 562, 604, 862
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 3 cut(s) 318, 376, 745
MhlI GDGCHC 2 cut(s) 536, 844
MluCI AATT 6 cut(s) 199, 572, 579, 673, 698, 960
MluI ACGCGT 3 cut(s) 327, 663, 858
MlyI GAGTC 2 cut(s) 126, 977
MmeI TCCRAC 4 cut(s) 38, 345, 723, 906
MroI TCCGGA 2 cut(s) 460, 467
MroNI GCCGGC 2 cut(s) 514, 804
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 153
MspR9I CCNGG 3 cut(s) 21, 314, 947
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 1 cut(s) 947
MvnI CGCG 6 cut(s) 139, 153, 329, 512, 665, 860
MwoI GCNNNNNNNGC 5 cut(s) 145, 191, 243, 260, 292
NaeI GCCGGC 2 cut(s) 516, 806
NciI CCSGG 2 cut(s) 21, 314
NdeII GATC 6 cut(s) 111, 318, 376, 711, 745, 873
NgoMIV GCCGGC 2 cut(s) 514, 804
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 247, 311, 320
NmuCI GTSAC 3 cut(s) 435, 491, 524
PaeR7I CTCGAG 1 cut(s) 287
PctI GAATGC 1 cut(s) 922
PdiI GCCGGC 2 cut(s) 516, 806
PfeI GAWTC 6 cut(s) 55, 80, 422, 464, 485, 935
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 5 cut(s) 125, 155, 187, 309, 906
PleI GAGTC 2 cut(s) 126, 976
PpsI GAGTC 2 cut(s) 126, 976
PshAI GACNNNNGTC 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 945
PspEI GGTNACC 4 cut(s) 435, 449, 491, 524
PspGI CCWGG 1 cut(s) 945
PspN4I GGNNCC 3 cut(s) 247, 311, 320
PspPI GGNCC 2 cut(s) 172, 347
PspXI VCTCGAGB 1 cut(s) 287
PsuI RGATCY 3 cut(s) 318, 376, 745
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 915
SacII CCGCGG 1 cut(s) 154
SatI GCNGC 5 cut(s) 124, 154, 186, 308, 905
Sau3AI GATC 6 cut(s) 111, 318, 376, 711, 745, 873
Sau96I GGNCC 2 cut(s) 172, 347
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 126, 977
ScrFI CCNGG 3 cut(s) 21, 314, 947
SduI GDGCHC 2 cut(s) 536, 844
Sfr274I CTCGAG 1 cut(s) 287
Sfr303I CCGCGG 1 cut(s) 154
SgrBI CCGCGG 1 cut(s) 154
SinI GGWCC 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 3 cut(s) 143, 269, 287
SmoI CTYRAG 3 cut(s) 143, 269, 287
Sse9I AATT 6 cut(s) 199, 572, 579, 673, 698, 960
SsiI CCGC 7 cut(s) 151, 153, 185, 307, 380, 510, 707
SspMI CTAG 2 cut(s) 813, 965
StyD4I CCNGG 3 cut(s) 19, 312, 945
StyI CCWWGG 2 cut(s) 249, 636
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 782
TaqI TCGA 6 cut(s) 58, 78, 288, 389, 558, 957
TaqII GACCGA 2 cut(s) 423, 465
TasI AATT 6 cut(s) 199, 572, 579, 673, 698, 960
TatI WGTACW 1 cut(s) 273
TauI GCSGC 3 cut(s) 156, 188, 310
TfiI GAWTC 6 cut(s) 55, 80, 422, 464, 485, 935
TscAI CASTG 3 cut(s) 526, 606, 849
TseFI GTSAC 3 cut(s) 435, 491, 524
TseI GCWGC 2 cut(s) 123, 904
Tsp45I GTSAC 3 cut(s) 435, 491, 524
TspDTI ATGAA 2 cut(s) 76, 272
TspGWI ACGGA 5 cut(s) 106, 185, 214, 659, 762
TspRI CASTG 3 cut(s) 526, 606, 849
VneI GTGCAC 1 cut(s) 840
VpaK11BI GGWCC 1 cut(s) 172
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 2 cut(s) 199, 960
XbaI TCTAGA 2 cut(s) 812, 964
XhoI CTCGAG 1 cut(s) 287
XspI CTAG 2 cut(s) 813, 965
ZraI GACGTC 1 cut(s) 780
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.