Rmu_sc0002923.1_g000033

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002923.1
OG0002637
Physical Location & Seq
Forward (+)
139865 .. 140711
847 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atggcccagattcccaagttggacaatgctcctgtaaatctcaaatatatgagggaacagtctcagaaggagctcataaacatccttaagaatattcgaggaaggaagtgtttggtgattgaccccaagctcagtggttcaatatcattgatcattcagactgcacttctcaaagaacttgaggttgaactgcgacatctttcggctgaaacaattcaaactgactgttccaaagtggttttccttgtccgttctcagcttagtttgatgaaatacattagttcacaagtccaaaatgatacttcgaaaggtgttgcttgtgagaagatccttgaggaggaaaaagtccatgacttgttgagtataggggagtaccagctatacagtgttccattggatgaggatgttttgtcatttgaacttgatctctctctcaaggaatgccttgttgatggtgatatgagctcactttggcatattgcgaaagccattcacaagcttgagttttcttttggggtgaaaccaaatgtgagggcgaaaggaaatgcatcggtgcgtgttgctgacattctaaacggcatgcaagctgaggaacccgttagctcatcagatacgttaacatatgagggcattattgatgagtttctgcgtatcaatgatggttctgttgaggttgatccatccttcatggatgtcaaacaagaagaaaagaagatgaattga

Protein Analysis

240

Amino Acids

26.97

Weight (kDa)

5.01

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 36 - 193 2.6e-17 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 322, 671
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 337
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 4 cut(s) 141, 188, 218, 419
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 8 cut(s) 73, 130, 259, 379, 465, 499, 587, 603
AluI AGCT 8 cut(s) 73, 130, 259, 379, 465, 499, 587, 603
Alw21I GWGCWC 2 cut(s) 75, 467
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 2 cut(s) 322, 671
AoxI GGCC 1 cut(s) 3
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 127, 467, 529
AsuII TTCGAA 1 cut(s) 305
BanII GRGCYC 2 cut(s) 75, 467
Bbv12I GWGCWC 2 cut(s) 75, 467
BbvCI CCTCAGC 1 cut(s) 588
BccI CCATC 3 cut(s) 446, 653, 688
BceAI ACGGC 1 cut(s) 592
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 66
BfrI CTTAAG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 594
BmsI GCATC 1 cut(s) 557
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 305
BpuEI CTTGAG 4 cut(s) 200, 353, 419, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 337
BseGI GGATG 5 cut(s) 81, 403, 409, 680, 697
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMII CTCAG 4 cut(s) 77, 145, 269, 579
BseRI GAGGAG 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 75, 467
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 75, 467
Bsp143I GATC 4 cut(s) 150, 327, 424, 676
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 76, 144, 268, 580
BspLI GGNNCC 1 cut(s) 594
BspPI GGATC 2 cut(s) 322, 671
BspT104I TTCGAA 1 cut(s) 305
BspTI CTTAAG 1 cut(s) 86
BssMI GATC 4 cut(s) 150, 327, 424, 676
Bst4CI ACNGT 3 cut(s) 60, 227, 386
BstAFI CTTAAG 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 581, 585
BstDEI CTNAG 5 cut(s) 63, 131, 255, 260, 588
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 5 cut(s) 81, 403, 409, 680, 697
BstKTI GATC 4 cut(s) 153, 330, 427, 679
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 4 cut(s) 150, 327, 424, 676
BstNSI RCATGY 1 cut(s) 583
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 5 cut(s) 81, 403, 409, 680, 697
BtsIMutI CAGTG 2 cut(s) 139, 391
Cac8I GCNNGC 2 cut(s) 581, 585
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 3 cut(s) 350, 580, 688
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 5 cut(s) 63, 131, 255, 260, 588
DpnI GATC 4 cut(s) 152, 329, 426, 678
DpnII GATC 4 cut(s) 150, 327, 424, 676
Ecl136II GAGCTC 2 cut(s) 73, 465
Eco24I GRGCYC 2 cut(s) 75, 467
Eco53kI GAGCTC 2 cut(s) 73, 465
EcoICRI GAGCTC 2 cut(s) 73, 465
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 75, 467
FaeI CATG 3 cut(s) 353, 583, 691
FatI CATG 3 cut(s) 349, 579, 687
FauNDI CATATG 1 cut(s) 622
FbaI TGATCA 1 cut(s) 150
FokI GGATG 5 cut(s) 68, 410, 416, 667, 704
FriOI GRGCYC 2 cut(s) 75, 467
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 3 cut(s) 353, 583, 691
HincII GTYRAC 1 cut(s) 618
HindII GTYRAC 1 cut(s) 618
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 10
HpaI GTTAAC 1 cut(s) 618
HphI GGTGA 3 cut(s) 127, 467, 529
Hpy166II GTNNAC 2 cut(s) 284, 618
Hpy188I TCNGA 3 cut(s) 66, 159, 610
Hpy8I GTNNAC 2 cut(s) 284, 618
HpyAV CCTTC 3 cut(s) 61, 96, 694
HpyCH4III ACNGT 3 cut(s) 60, 227, 386
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 3 cut(s) 164, 548, 583
HpyF3I CTNAG 5 cut(s) 63, 131, 255, 260, 588
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 3 cut(s) 353, 583, 691
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 618
Kzo9I GATC 4 cut(s) 150, 327, 424, 676
LmnI GCTCC 2 cut(s) 34, 70
LpnPI CCDG 3 cut(s) 20, 45, 389
LweI GCATC 1 cut(s) 557
MaeII ACGT 1 cut(s) 614
MalI GATC 4 cut(s) 152, 329, 426, 678
MboI GATC 4 cut(s) 150, 327, 424, 676
MboII GAAGA 2 cut(s) 337, 716
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 2 cut(s) 75, 467
MluCI AATT 2 cut(s) 213, 718
Mph1103I ATGCAT 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 2 cut(s) 87, 617
MspCI CTTAAG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 446
NdeI CATATG 1 cut(s) 622
NdeII GATC 4 cut(s) 150, 327, 424, 676
NlaIII CATG 3 cut(s) 353, 583, 691
NlaIV GGNNCC 1 cut(s) 594
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 583
NspV TTCGAA 1 cut(s) 305
PaeI GCATGC 1 cut(s) 583
PctI GAATGC 1 cut(s) 446
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 10
Psp124BI GAGCTC 2 cut(s) 75, 467
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SacI GAGCTC 2 cut(s) 75, 467
SaqAI TTAA 2 cut(s) 87, 617
Sau3AI GATC 4 cut(s) 150, 327, 424, 676
Sau96I GGNCC 1 cut(s) 4
SduI GDGCHC 2 cut(s) 75, 467
SfaNI GCATC 1 cut(s) 557
SfuI TTCGAA 1 cut(s) 305
SmlI CTYRAG 5 cut(s) 86, 179, 332, 434, 500
SmoI CTYRAG 5 cut(s) 86, 179, 332, 434, 500
SphI GCATGC 1 cut(s) 583
Sse9I AATT 2 cut(s) 213, 718
SspI AATATT 1 cut(s) 94
SstI GAGCTC 2 cut(s) 75, 467
TaaI ACNGT 3 cut(s) 60, 227, 386
TaiI ACGT 1 cut(s) 617
TaqI TCGA 2 cut(s) 97, 305
TasI AATT 2 cut(s) 213, 718
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 2 cut(s) 87, 617
Tru9I TTAA 2 cut(s) 87, 617
TscAI CASTG 2 cut(s) 139, 391
TspDTI ATGAA 2 cut(s) 284, 676
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 2 cut(s) 139, 391
Vha464I CTTAAG 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 335
XceI RCATGY 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.