Rmu_sc0002923.1_g000034

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002923.1
OG0002637
Physical Location & Seq
Forward (+)
140879 .. 141681
803 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
atgcactcattgccagaagtgacaaggcacataaatcttgctaagcatctgatgactttcacgtcgaagccatcatttcgtggactacttgacgtggaacaaacaatcgttgagactgagagttttgacatatgcttagattacattgaagaaatgatgcataagcaggagcctcttgagacaaaaggcaagcggctgagagttaagcgtgccttacaacttgtcattgaacacagtgacacagctaattccaatgacattgactatgtcttttctggatatgcacctcttagcattcgccttgttcagcatgctattcaatctggatggcgtccgattgaagaaactctaaagctgttacccggaccacattcagaaacaaaaagaggcccgttctcaagcagcgcatcatttgacaattttcagggggcagaaaccaatgtagacaagctagggatcgcatatgatttcatagttgggacaacgaaaatggttagtggccatagcttgactgaaacatttgtggggaagatggaggacctgtaa

Protein Analysis

181

Amino Acids

20.41

Weight (kDa)

5.73

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 7 - 67 7.7e-06 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 444
AciI CCGC 1 cut(s) 193
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 499
AcyI GRCGYC 1 cut(s) 331
AgsI TTSAA 4 cut(s) 149, 230, 320, 341
AjiI CACGTC 2 cut(s) 63, 94
AluBI AGCT 4 cut(s) 245, 355, 451, 507
AluI AGCT 4 cut(s) 245, 355, 451, 507
Alw26I GTCTC 2 cut(s) 107, 173
AlwI GGATC 1 cut(s) 464
AoxI GGCC 2 cut(s) 388, 499
ApeKI GCWGC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 3 cut(s) 365, 389, 538
AsuC2I CCSGG 1 cut(s) 363
AvaII GGWCC 2 cut(s) 365, 538
BalI TGGCCA 1 cut(s) 501
BbvI GCAGC 1 cut(s) 414
BccI CCATC 3 cut(s) 79, 321, 526
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 107, 173
BfaI CTAG 1 cut(s) 452
BisI GCNGC 2 cut(s) 194, 403
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 2 cut(s) 195, 404
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 365, 538
BmgBI CACGTC 2 cut(s) 63, 94
BmgT120I GGNCC 3 cut(s) 365, 389, 538
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 3 cut(s) 55, 147, 416
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 197, 382
BpuMI CCSGG 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 331
BsaXI ACNNNNNCTCC 1 cut(s) 527
Bse3DI GCAATG 1 cut(s) 8
BseGI GGATG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 8
BseMII CTCAG 2 cut(s) 108, 188
BseXI GCAGC 1 cut(s) 414
BshFI GGCC 2 cut(s) 390, 501
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 1 cut(s) 493
BsmAI GTCTC 2 cut(s) 107, 173
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 2 cut(s) 390, 501
Bsp143I GATC 1 cut(s) 456
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 1 cut(s) 193
BspANI GGCC 2 cut(s) 390, 501
BspCNI CTCAG 2 cut(s) 109, 189
BspLI GGNNCC 1 cut(s) 171
BspPI GGATC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 8
BssMI GATC 1 cut(s) 456
BssNI GRCGYC 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 331
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 191, 210, 312
BstDEI CTNAG 5 cut(s) 42, 117, 136, 197, 290
BstF5I GGATG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 1 cut(s) 459
BstMAI GTCTC 2 cut(s) 107, 173
BstMBI GATC 1 cut(s) 456
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 361
BstV1I GCAGC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 390, 501
BtrI CACGTC 2 cut(s) 63, 94
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 3 cut(s) 191, 210, 312
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 365, 389, 538
CseI GACGC 1 cut(s) 320
CviAII CATG 1 cut(s) 311
CviJI RGCY 9 cut(s) 70, 172, 196, 245, 355, 390, 451, 501, 507
CviKI_1 RGCY 9 cut(s) 70, 172, 196, 245, 355, 390, 451, 501, 507
DdeI CTNAG 5 cut(s) 42, 117, 136, 197, 290
DpnI GATC 1 cut(s) 458
DpnII GATC 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 365, 538
EcoO109I RGGNCCY 1 cut(s) 538
EcoT22I ATGCAT 1 cut(s) 162
FaeI CATG 1 cut(s) 314
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 310
FauNDI CATATG 2 cut(s) 131, 463
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 194, 403
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 2 cut(s) 194, 403
FspBI CTAG 1 cut(s) 452
GlaI GCGC 1 cut(s) 406
GluI GCNGC 2 cut(s) 194, 403
HaeIII GGCC 2 cut(s) 390, 501
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HpaII CCGG 1 cut(s) 363
Hpy166II GTNNAC 2 cut(s) 83, 445
Hpy188I TCNGA 3 cut(s) 51, 336, 376
Hpy188III TCNNGA 3 cut(s) 176, 276, 324
Hpy8I GTNNAC 2 cut(s) 83, 445
Hpy99I CGWCG 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 2 cut(s) 62, 93
HpyCH4V TGCA 3 cut(s) 4, 160, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 5 cut(s) 42, 117, 136, 197, 290
HpySE526I ACGT 2 cut(s) 62, 93
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 456
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 6 cut(s) 27, 152, 261, 309, 376, 410
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 3 cut(s) 55, 147, 416
MaeI CTAG 1 cut(s) 452
MaeII ACGT 2 cut(s) 62, 93
MaeIII GTNAC 3 cut(s) 19, 236, 357
MalI GATC 1 cut(s) 458
MboI GATC 1 cut(s) 456
MboII GAAGA 3 cut(s) 161, 353, 541
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 2 cut(s) 247, 418
MluNI TGGCCA 1 cut(s) 501
MnlI CCTC 4 cut(s) 183, 297, 380, 529
Mox20I TGGCCA 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 162
MscI TGGCCA 1 cut(s) 501
MseI TTAA 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 501
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 363
NdeI CATATG 2 cut(s) 131, 463
NdeII GATC 1 cut(s) 456
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 2 cut(s) 19, 236
NsiI ATGCAT 1 cut(s) 162
NspI RCATGY 1 cut(s) 314
PaeI GCATGC 1 cut(s) 314
PctI GAATGC 1 cut(s) 294
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 2 cut(s) 195, 404
PpuMI RGGWCCY 1 cut(s) 538
Psp5II RGGWCCY 1 cut(s) 538
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 3 cut(s) 365, 389, 538
PspPPI RGGWCCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 194, 403
Sau3AI GATC 1 cut(s) 456
Sau96I GGNCC 3 cut(s) 365, 389, 538
ScrFI CCNGG 1 cut(s) 363
SetI ASST 8 cut(s) 65, 96, 247, 289, 357, 453, 509, 543
SfaNI GCATC 3 cut(s) 55, 147, 416
SinI GGWCC 2 cut(s) 365, 538
SmlI CTYRAG 2 cut(s) 176, 397
SmoI CTYRAG 2 cut(s) 176, 397
SphI GCATGC 1 cut(s) 314
Sse9I AATT 2 cut(s) 247, 418
SsiI CCGC 1 cut(s) 193
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 2 cut(s) 65, 96
TaqI TCGA 1 cut(s) 65
TasI AATT 2 cut(s) 247, 418
TauI GCSGC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 2 cut(s) 19, 236
TseI GCWGC 1 cut(s) 402
Tsp45I GTSAC 2 cut(s) 19, 236
TspDTI ATGAA 1 cut(s) 460
TspRI CASTG 1 cut(s) 241
Tth111I GACNNNGTC 1 cut(s) 266
VpaK11BI GGWCC 2 cut(s) 365, 538
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.