Rmu_sc0003005.1_g000017
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003005.1
OG0002724
Physical Location & Seq
Reverse (-)
75007 .. 77442
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1233 bp
atggatgaacaacgctgtgacttgaaacacttgcactcggaccagagctttctggggctcagtactacttccgatcatcgtctagttgaacccccaataaaagaagaaacattattcaaagagatcaaagaagttgatttcttttccaataatagacttcccgatcaaccggcgcctggtcatgccgagaaccatgttggaccaaatcaccaaaagctacaatgggttgcaggctcagctgcgcatgatcagacttcaagagcagctgaagacagcattaatcttgatccgaagttatccttcctccatgatgaccaatcccccaaaagtagttctcatcacgatcatgatcaagaacaagttcttgctgctactaatcatgatcaggtttcttcaagaagggctagggtctccgtacgagcaagatcggaagctcccatgattagagatggatgtcaatggaggaaatacggtcaaaagatggccaagggtaatccttgtccccgtgcttactatcgctgcactatgttcgctggatgccccgttcggaaacaggtgcaaagaatggcagaggacaagaccattctgataacaacatatgaaggtaatcacaaccaccctcttcctgcagctgcgatatctatggctaataccacatcagcagcagcagccatgttgatatcgggttcaacaaccagcaaggaagctcatcatcatctaacacaccctacaggcttgttctcttctcttcagccgctctatggttcatccatggccaccttttcaacctctgcaccatttcccacaatcacactggacatgaccggcacccctaaccgtaaccctaatcccatgcagttcctggacagtagtgccacatttcctaatcctttgcagggtaatagtcatccgcttatagggcaccccatgtgtttgaatttccctcagcataatcatgcagcgctaacacaagcggcaatgcaactagcagaacaaccccacggttcgatggttgagacaataactgcagccattacttctgacccgaagttcactacagccctagctgctgccatctcaagtagaataaataatgttgaggatattaatgctggattacaaggaaataccaatggtattagttcccttaacagtaatggaaatgaggtttgcgcagcattatctggctccccacagcttcctcaatcctgtaccactttctccaccacctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

410

Amino Acids

44.52

Weight (kDa)

6.43

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 150 - 207 2.1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 243, 1174
AccB1I GGYRCC 3 cut(s) 172, 827, 921
AccBSI CCGCTC 1 cut(s) 757
AciI CCGC 3 cut(s) 755, 911, 974
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 2 cut(s) 483, 774
AcsI RAATTY 1 cut(s) 937
AcuI CTGAAG 2 cut(s) 288, 734
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 3 cut(s) 64, 417, 1213
AfeI AGCGCT 1 cut(s) 963
AfiI CCNNNNNNNGG 4 cut(s) 176, 761, 896, 917
AgsI TTSAA 8 cut(s) 25, 89, 118, 258, 396, 690, 786, 937
AjnI CCWGG 2 cut(s) 175, 861
AloI GAACNNNNNNTCC 2 cut(s) 528, 560
AluBI AGCT 9 cut(s) 48, 217, 239, 266, 434, 632, 707, 1067, 1198
AluI AGCT 9 cut(s) 48, 217, 239, 266, 434, 632, 707, 1067, 1198
Alw26I GTCTC 2 cut(s) 415, 1010
AlwI GGATC 1 cut(s) 281
AlwNI CAGNNNCTG 1 cut(s) 862
Aor51HI AGCGCT 1 cut(s) 963
AoxI GGCC 2 cut(s) 483, 774
ApoI RAATTY 1 cut(s) 937
AseI ATTAAT 2 cut(s) 279, 1107
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 4 cut(s) 175, 244, 964, 1175
AspS9I GGNCC 2 cut(s) 40, 200
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 40, 200
BaeGI GKGCMC 1 cut(s) 924
BalI TGGCCA 2 cut(s) 485, 776
BanI GGYRCC 3 cut(s) 172, 827, 921
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 945
BccI CCATC 4 cut(s) 443, 475, 1003, 1082
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 2 cut(s) 177, 863
BclI TGATCA 3 cut(s) 247, 349, 382
BcoDI GTCTC 2 cut(s) 415, 1010
BfaI CTAG 4 cut(s) 83, 405, 986, 1064
BfmI CTRYAG 4 cut(s) 627, 729, 1026, 1056
BfoI RGCGCY 2 cut(s) 176, 965
BlpI GCTNAGC 1 cut(s) 235
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 177, 863
Bme18I GGWCC 2 cut(s) 40, 200
BmgT120I GGNCC 2 cut(s) 40, 200
BmiI GGNNCC 4 cut(s) 174, 829, 923, 1189
BmrFI CCNGG 2 cut(s) 177, 863
BmsI GCATC 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 945
Bpu1102I GCTNAGC 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 1063
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 3 cut(s) 486, 771, 1000
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 761, 896, 917
Bse118I RCCGGY 2 cut(s) 169, 824
Bse1I ACTGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 984
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 2 cut(s) 177, 863
BseDI CCNNGG 3 cut(s) 486, 771, 1000
BseGI GGATG 5 cut(s) 10, 458, 542, 767, 907
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 4 cut(s) 176, 761, 896, 917
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 3 cut(s) 73, 249, 959
BseNI ACTGG 1 cut(s) 819
BseSI GKGCMC 1 cut(s) 924
BsgI GTGCAG 2 cut(s) 505, 777
BshFI GGCC 2 cut(s) 485, 776
BshNI GGYRCC 3 cut(s) 172, 827, 921
BsiSI CCGG 2 cut(s) 170, 825
BsiWI CGTACG 1 cut(s) 415
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 4 cut(s) 176, 761, 896, 917
BsmAI GTCTC 2 cut(s) 415, 1010
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 2 cut(s) 485, 776
Bso31I GGTCTC 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 60, 924
Bsp143I GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
Bsp1720I GCTNAGC 1 cut(s) 235
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 3 cut(s) 755, 911, 974
BspANI GGCC 2 cut(s) 485, 776
BspCNI CTCAG 3 cut(s) 72, 248, 958
BspHI TCATGA 2 cut(s) 346, 379
BspLI GGNNCC 4 cut(s) 174, 829, 923, 1189
BspMAI CTGCAG 2 cut(s) 631, 1030
BspPI GGATC 1 cut(s) 281
BspT107I GGYRCC 3 cut(s) 172, 827, 921
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 1 cut(s) 757
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 2 cut(s) 169, 824
BsrI ACTGG 1 cut(s) 819
BssAI RCCGGY 2 cut(s) 169, 824
BssECI CCNNGG 3 cut(s) 486, 771, 1000
BssMI GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 486, 771
Bst2UI CCWGG 2 cut(s) 177, 863
Bst4CI ACNGT 5 cut(s) 473, 839, 869, 1004, 1154
Bst6I CTCTTC 3 cut(s) 627, 748, 753
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 3 cut(s) 59, 235, 945
BstDSI CCRYGG 2 cut(s) 771, 1000
BstF5I GGATG 5 cut(s) 10, 458, 542, 767, 907
BstH2I RGCGCY 2 cut(s) 176, 965
BstHHI GCGC 4 cut(s) 175, 244, 964, 1175
BstKTI GATC 9 cut(s) 76, 126, 166, 250, 289, 346, 352, 385, 428
BstMAI GTCTC 2 cut(s) 415, 1010
BstMBI GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
BstMWI GCNNNNNNNGC 4 cut(s) 236, 668, 919, 1067
BstNI CCWGG 2 cut(s) 177, 863
BstSCI CCNGG 2 cut(s) 175, 861
BstSFI CTRYAG 4 cut(s) 627, 729, 1026, 1056
BstSLI GKGCMC 1 cut(s) 924
BstV2I GAAGAC 1 cut(s) 276
BsuRI GGCC 2 cut(s) 485, 776
BtgI CCRYGG 2 cut(s) 771, 1000
BtsCI GGATG 5 cut(s) 10, 458, 542, 767, 907
BtsIMutI CAGTG 1 cut(s) 812
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 862
CciI TCATGA 2 cut(s) 346, 379
CfoI GCGC 4 cut(s) 175, 244, 964, 1175
Cfr10I RCCGGY 2 cut(s) 169, 824
Cfr13I GGNCC 2 cut(s) 40, 200
Csp6I GTAC 3 cut(s) 63, 416, 1212
CviQI GTAC 3 cut(s) 63, 416, 1212
DdeI CTNAG 3 cut(s) 59, 235, 945
DinI GGCGCC 1 cut(s) 174
DpnI GATC 9 cut(s) 75, 125, 165, 249, 288, 345, 351, 384, 427
DpnII GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
EaeI YGGCCR 2 cut(s) 483, 774
Eam1104I CTCTTC 3 cut(s) 627, 748, 753
EarI CTCTTC 3 cut(s) 627, 748, 753
Eco130I CCWWGG 2 cut(s) 486, 771
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 2 cut(s) 639, 681
Eco47I GGWCC 2 cut(s) 40, 200
Eco47III AGCGCT 1 cut(s) 963
Eco57I CTGAAG 2 cut(s) 288, 734
EcoRII CCWGG 2 cut(s) 175, 861
EcoRV GATATC 2 cut(s) 639, 681
EcoT14I CCWWGG 2 cut(s) 486, 771
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 486, 771
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 1 cut(s) 486
FauNDI CATATG 1 cut(s) 598
FbaI TGATCA 3 cut(s) 247, 349, 382
FokI GGATG 5 cut(s) 17, 465, 549, 754, 894
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 4 cut(s) 83, 405, 986, 1064
FspI TGCGCA 2 cut(s) 243, 1174
GlaI GCGC 4 cut(s) 174, 243, 963, 1174
HaeII RGCGCY 2 cut(s) 176, 965
HaeIII GGCC 2 cut(s) 485, 776
HapII CCGG 2 cut(s) 170, 825
HhaI GCGC 4 cut(s) 175, 244, 964, 1175
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 4 cut(s) 173, 242, 962, 1173
HinP1I GCGC 4 cut(s) 173, 242, 962, 1173
HpaII CCGG 2 cut(s) 170, 825
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 1053
Hpy188I TCNGA 8 cut(s) 40, 73, 252, 291, 430, 549, 588, 1042
Hpy188III TCNNGA 8 cut(s) 161, 258, 284, 341, 347, 353, 380, 396
Hpy8I GTNNAC 1 cut(s) 1053
HpyAV CCTTC 3 cut(s) 310, 393, 596
HpyCH4III ACNGT 5 cut(s) 473, 839, 869, 1004, 1154
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 668, 919, 1067
HpyF3I CTNAG 3 cut(s) 59, 235, 945
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 4 cut(s) 173, 242, 962, 1173
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 3 cut(s) 247, 349, 382
Kzo9I GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
LmnI GCTCC 2 cut(s) 439, 1193
LweI GCATC 1 cut(s) 527
MaeI CTAG 4 cut(s) 83, 405, 986, 1064
MaeIII GTNAC 2 cut(s) 17, 839
MalI GATC 9 cut(s) 75, 125, 165, 249, 288, 345, 351, 384, 427
MbiI CCGCTC 1 cut(s) 757
MboI GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
MboII GAAGA 6 cut(s) 116, 281, 384, 614, 735, 740
MhlI GDGCHC 2 cut(s) 60, 924
MlsI TGGCCA 2 cut(s) 485, 776
MluCI AATT 1 cut(s) 937
MluNI TGGCCA 2 cut(s) 485, 776
Mly113I GGCGCC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 9 cut(s) 314, 456, 565, 630, 799, 954, 1093, 1159, 1212
Mox20I TGGCCA 2 cut(s) 485, 776
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 2 cut(s) 485, 776
MseI TTAA 3 cut(s) 279, 1107, 1149
MslI CAYNNNNRTG 2 cut(s) 345, 954
Msp20I TGGCCA 2 cut(s) 485, 776
MspA1I CMGCKG 3 cut(s) 239, 266, 632
MspI CCGG 2 cut(s) 170, 825
MspR9I CCNGG 2 cut(s) 177, 863
MvaI CCWGG 2 cut(s) 177, 863
MwoI GCNNNNNNNGC 4 cut(s) 236, 668, 919, 1067
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 771
NdeI CATATG 1 cut(s) 598
NdeII GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
NlaIV GGNNCC 4 cut(s) 174, 829, 923, 1189
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 2 cut(s) 243, 1174
PagI TCATGA 2 cut(s) 346, 379
PdmI GAANNNNTTC 1 cut(s) 360
Pfl23II CGTACG 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 861
PluTI GGCGCC 1 cut(s) 176
PshBI ATTAAT 2 cut(s) 279, 1107
Psp6I CCWGG 2 cut(s) 175, 861
PspGI CCWGG 2 cut(s) 175, 861
PspLI CGTACG 1 cut(s) 415
PspN4I GGNNCC 4 cut(s) 174, 829, 923, 1189
PspPI GGNCC 2 cut(s) 40, 200
PstI CTGCAG 2 cut(s) 631, 1030
PstNI CAGNNNCTG 1 cut(s) 862
PvuII CAGCTG 3 cut(s) 239, 266, 632
RsaI GTAC 3 cut(s) 64, 417, 1213
RsaNI GTAC 3 cut(s) 63, 416, 1212
RseI CAYNNNNRTG 2 cut(s) 345, 954
SaqAI TTAA 3 cut(s) 279, 1107, 1149
Sau3AI GATC 9 cut(s) 73, 123, 163, 247, 286, 343, 349, 382, 425
Sau96I GGNCC 2 cut(s) 40, 200
ScaI AGTACT 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 177, 863
SduI GDGCHC 2 cut(s) 60, 924
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 4 cut(s) 627, 729, 1026, 1056
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 40, 200
SmiMI CAYNNNNRTG 2 cut(s) 345, 954
SmlI CTYRAG 1 cut(s) 1078
SmoI CTYRAG 1 cut(s) 1078
Sse9I AATT 1 cut(s) 937
SsiI CCGC 3 cut(s) 755, 911, 974
SspDI GGCGCC 1 cut(s) 172
SspMI CTAG 4 cut(s) 83, 405, 986, 1064
StyD4I CCNGG 2 cut(s) 175, 861
StyI CCWWGG 2 cut(s) 486, 771
TaaI ACNGT 5 cut(s) 473, 839, 869, 1004, 1154
TaqI TCGA 1 cut(s) 1007
TasI AATT 1 cut(s) 937
TatI WGTACW 1 cut(s) 62
TauI GCSGC 2 cut(s) 757, 977
Tru1I TTAA 3 cut(s) 279, 1107, 1149
Tru9I TTAA 3 cut(s) 279, 1107, 1149
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 3 cut(s) 21, 615, 756
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 819
VpaK11BI GGWCC 2 cut(s) 40, 200
VspI ATTAAT 2 cut(s) 279, 1107
XapI RAATTY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 2 cut(s) 811, 859
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 4 cut(s) 83, 405, 986, 1064
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.