Rmu_sc0003115.1_g000045

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003115.1
OG0003157
Physical Location & Seq
Forward (+)
230023 .. 232265
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
atgaacattcttgtccgacagcggccctggcctaggctggccttttggcctcgccaaagctacatggctttgttgccggtagttgtgttgccagggattggaggaactcgttttgacaacgttggtttgaggtttcgaagaagtctggacaatgttagtcgtggcggcatggtagattcaatgagctccaacacaactgagacgaagaaggagaagaagggtcctcctcaaattgttaaattgaataaagctttggctttggccgaaaaatgggtgagtaacatgagtaaactgccagcagaagatgagtcttttgagacacagagtcgacctgctaggcttggactaggtgctaaagttccccgtgcatccacatttgtgccttcagatgatccccttgaaagaaagttatattacaagttgaatgctcaaaagagaaaagctgccaaaattgcagaggagttagcaccagctgctgcagatggtgatgatgacgatgaagataatgaagacagcagaacccgtgcgtttgagaagaaaaagagagcagctaatccactgagcccacctttagggaaaaagaaaaggagaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

198

Amino Acids

22.2

Weight (kDa)

10.13

Isoelectric Point (pI)

65.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 77 - 196 2.5e-12 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AccB7I CCANNNNNTGG 1 cut(s) 98
AccI GTMKAC 1 cut(s) 328
AciI CCGC 2 cut(s) 22, 165
AclI AACGTT 1 cut(s) 120
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 369
AfiI CCNNNNNNNGG 4 cut(s) 33, 98, 270, 572
AgsI TTSAA 4 cut(s) 180, 244, 401, 424
AhdI GACNNNNNGTC 1 cut(s) 324
AjnI CCWGG 2 cut(s) 26, 91
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 6 cut(s) 60, 186, 251, 443, 473, 551
AluI AGCT 6 cut(s) 60, 186, 251, 443, 473, 551
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 194, 311
AlwI GGATC 1 cut(s) 386
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 5 cut(s) 23, 29, 39, 47, 261
ApeKI GCWGC 4 cut(s) 443, 473, 476, 548
AspA2I CCTAGG 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 24, 221
AsuHPI GGTGA 2 cut(s) 286, 497
AsuII TTCGAA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 221
AvrII CCTAGG 1 cut(s) 32
BanII GRGCYC 2 cut(s) 188, 566
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 430, 460, 463, 560
BccI CCATC 1 cut(s) 476
BciT130I CCWGG 2 cut(s) 28, 93
BcoDI GTCTC 2 cut(s) 194, 311
BfaI CTAG 3 cut(s) 33, 336, 347
BfmI CTRYAG 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 6 cut(s) 23, 166, 444, 474, 477, 549
BlnI CCTAGG 1 cut(s) 32
BlsI GCNGC 6 cut(s) 24, 167, 445, 475, 478, 550
Bme1390I CCNGG 2 cut(s) 28, 93
Bme18I GGWCC 1 cut(s) 221
BmeRI GACNNNNNGTC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 24, 221
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 28, 93
BmsI GCATC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 26, 32, 92
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 98, 270, 572
Bse118I RCCGGY 1 cut(s) 76
BseBI CCWGG 2 cut(s) 28, 93
BseDI CCNNGG 3 cut(s) 26, 32, 92
BseGI GGATG 1 cut(s) 368
BseLI CCNNNNNNNGG 4 cut(s) 33, 98, 270, 572
BseMII CTCAG 2 cut(s) 189, 551
BseRI GAGGAG 2 cut(s) 216, 473
BseXI GCAGC 4 cut(s) 430, 460, 463, 560
BshFI GGCC 5 cut(s) 25, 31, 41, 49, 263
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 4 cut(s) 33, 98, 270, 572
BsmAI GTCTC 2 cut(s) 194, 311
BsmBI CGTCTC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 5 cut(s) 25, 31, 41, 49, 263
Bsp119I TTCGAA 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 188, 566
Bsp143I GATC 1 cut(s) 391
BspACI CCGC 2 cut(s) 22, 165
BspANI GGCC 5 cut(s) 25, 31, 41, 49, 263
BspCNI CTCAG 2 cut(s) 190, 552
BspLI GGNNCC 1 cut(s) 222
BspMAI CTGCAG 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 76
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 26, 32, 92
BssMI GATC 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 28, 93
BstAPI GCANNNNNTGC 1 cut(s) 473
BstBI TTCGAA 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 39, 297
BstDEI CTNAG 2 cut(s) 198, 560
BstENI CCTNNNNNAGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 368
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 2 cut(s) 194, 311
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 28, 452, 473
BstNI CCWGG 2 cut(s) 28, 93
BstSCI CCNGG 2 cut(s) 26, 91
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 430, 460, 463, 560
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 5 cut(s) 25, 31, 41, 49, 263
BtsCI GGATG 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 557
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 39, 297
CaiI CAGNNNCTG 1 cut(s) 476
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 24, 221
CviAII CATG 3 cut(s) 64, 169, 283
DdeI CTNAG 2 cut(s) 198, 560
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
DriI GACNNNNNGTC 1 cut(s) 324
EaeI YGGCCR 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 188, 566
Eco47I GGWCC 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 26, 91
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 2 cut(s) 188, 566
ErhI CCWWGG 1 cut(s) 32
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 3 cut(s) 67, 172, 286
FaiI YATR 4 cut(s) 65, 170, 284, 412
FatI CATG 3 cut(s) 63, 168, 282
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 6 cut(s) 23, 166, 444, 474, 477, 549
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 2 cut(s) 188, 566
Fsp4HI GCNGC 6 cut(s) 23, 166, 444, 474, 477, 549
FspBI CTAG 3 cut(s) 33, 336, 347
GluI GCNGC 6 cut(s) 23, 166, 444, 474, 477, 549
HaeIII GGCC 5 cut(s) 25, 31, 41, 49, 263
HapII CCGG 1 cut(s) 77
Hin1II CATG 3 cut(s) 67, 172, 286
HincII GTYRAC 1 cut(s) 329
HindII GTYRAC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 3 cut(s) 176, 308, 325
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 286, 497
Hpy166II GTNNAC 2 cut(s) 290, 329
Hpy188I TCNGA 2 cut(s) 17, 388
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 2 cut(s) 290, 329
HpyAV CCTTC 3 cut(s) 202, 211, 393
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 3 cut(s) 368, 455, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 452, 473
HpyF3I CTNAG 2 cut(s) 198, 560
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 3 cut(s) 67, 172, 286
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 4 cut(s) 430, 460, 463, 560
LweI GCATC 1 cut(s) 377
MaeI CTAG 3 cut(s) 33, 336, 347
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 278
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 7 cut(s) 150, 217, 226, 314, 512, 521, 547
MhlI GDGCHC 2 cut(s) 188, 566
MluCI AATT 3 cut(s) 231, 239, 450
MlyI GAGTC 2 cut(s) 317, 334
MmeI TCCRAC 2 cut(s) 40, 213
MnlI CCTC 6 cut(s) 60, 95, 123, 234, 237, 451
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 22, 473
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 28, 93
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 28, 93
MwoI GCNNNNNNNGC 3 cut(s) 28, 452, 473
NdeII GATC 1 cut(s) 391
NlaIII CATG 3 cut(s) 67, 172, 286
NlaIV GGNNCC 1 cut(s) 222
NspV TTCGAA 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 377
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 98
PkrI GCNGC 6 cut(s) 24, 167, 445, 475, 478, 550
PleI GAGTC 2 cut(s) 316, 333
PpsI GAGTC 2 cut(s) 316, 333
PpuMI RGGWCCY 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 188
Psp1406I AACGTT 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 26, 91
PspGI CCWGG 2 cut(s) 26, 91
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 2 cut(s) 24, 221
PspPPI RGGWCCY 1 cut(s) 221
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 188
SalI GTCGAC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 6 cut(s) 23, 166, 444, 474, 477, 549
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 2 cut(s) 24, 221
SchI GAGTC 2 cut(s) 317, 334
ScrFI CCNGG 2 cut(s) 28, 93
SduI GDGCHC 2 cut(s) 188, 566
SfaNI GCATC 1 cut(s) 377
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 136
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 377
Sse9I AATT 3 cut(s) 231, 239, 450
SsiI CCGC 2 cut(s) 22, 165
SspMI CTAG 3 cut(s) 33, 336, 347
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 26, 91
StyI CCWWGG 1 cut(s) 32
TaiI ACGT 1 cut(s) 123
TaqI TCGA 2 cut(s) 136, 328
TasI AATT 3 cut(s) 231, 239, 450
TauI GCSGC 2 cut(s) 25, 168
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 564
TseI GCWGC 4 cut(s) 443, 473, 476, 548
TspDTI ATGAA 3 cut(s) 17, 513, 522
TspRI CASTG 1 cut(s) 564
Van91I CCANNNNNTGG 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 221
XagI CCTNNNNNAGG 1 cut(s) 31
XmaJI CCTAGG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 3 cut(s) 33, 336, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.