Rmu_sc0003237.1_g000021

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003237.1
OG0004217
Physical Location & Seq
Forward (+)
79536 .. 81172
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
atgggaatgaagggagtgagaaagttaagtcgaaagatcttcaaaaacgtttgcaaacgtgatggagatctgtggagatcatacgaggacgcttgcaagaatgaaaaggatttcctaactgaactggacaagttcctagagtcgtataagaaatgtttgattcaaattaatgacgacagaggaattgatgatggttacatgaggattttggagaaattgaaaaatgtcaagggggcctcaggtagagtctatgatcagcaatttgcatatcaaattttgcccaaaaaggttgaatgtttgttgacgcagcgacgagatttgcaaaaagaattgaatgaccgaataccaaagcctcgcaagaagtatgattgtactcgtgcttggagacgctgtttgaggacggtcactcatatattgttcattggtgctgcagctgctgagtttgcctgcacagttgtggcagcagttatggctgctccacttgttgcactggccattgcagctgctattatcccaacactagccggacagcattggactctgtcttggattgaagattctgaaattcctttagagtattctgagtctgtaattcgcttaatgcaatccggaactgtgctttccattaaggaattgggagaaattcggacgtatctcggtagagttgtaaaggacatagaatcactcttatcccatctcaattctgtaattgaggaagatgaagatatcaatatgaaaattattgagcgtcttctaaaggacattaatgagttgaaggaaagagcaaggaatagcaagtccaagatattgaaggcatgcgatgaggagtccaaatccattggtggaaccactagaactggctggaaaatcactgaaccaaccaccagaagtcctgaaccggcccctttgtcagcctcgctcgcttcagctgctagcctcgcctccagccgccatttgccgagactgttagcctcatctcaacagcacagcgccccagcacctgttattacaggagccgccccggatttcaccttgaaatccaaagtggccctgcggggcccaccttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

355

Amino Acids

39.74

Weight (kDa)

9.25

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 64 - 263 1.4e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 608
AciI CCGC 3 cut(s) 949, 1017, 1054
AclI AACGTT 1 cut(s) 48
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 3 cut(s) 273, 564, 642
AcuI CTGAAG 1 cut(s) 909
AfaI GTAC 1 cut(s) 373
AgsI TTSAA 9 cut(s) 43, 164, 220, 293, 334, 554, 775, 811, 1036
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 3 cut(s) 434, 503, 929
AluI AGCT 3 cut(s) 434, 503, 929
Alw26I GTCTC 2 cut(s) 379, 955
AlwNI CAGNNNCTG 2 cut(s) 437, 1001
Aor13HI TCCGGA 1 cut(s) 608
AoxI GGCC 5 cut(s) 234, 492, 900, 1047, 1057
ApaI GGGCCC 1 cut(s) 1061
ApeKI GCWGC 9 cut(s) 307, 428, 431, 434, 461, 473, 500, 503, 929
ApoI RAATTY 3 cut(s) 273, 564, 642
AseI ATTAAT 2 cut(s) 168, 765
AspLEI GCGC 1 cut(s) 992
AspS9I GGNCC 5 cut(s) 234, 901, 1048, 1057, 1058
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 1 cut(s) 1021
AsuNHI GCTAGC 1 cut(s) 932
AxyI CCTNAGG 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 1061
BalI TGGCCA 1 cut(s) 494
BanII GRGCYC 1 cut(s) 1061
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 9 cut(s) 319, 415, 421, 443, 460, 473, 490, 512, 916
BccI CCATC 3 cut(s) 56, 185, 702
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 2 cut(s) 379, 955
BfaI CTAG 4 cut(s) 137, 521, 852, 933
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 993
BglII AGATCT 2 cut(s) 36, 67
Bme1390I CCNGG 1 cut(s) 1022
BmgT120I GGNCC 5 cut(s) 234, 901, 1048, 1057, 1058
BmiI GGNNCC 6 cut(s) 235, 847, 903, 1015, 1058, 1059
BmrFI CCNGG 1 cut(s) 1022
BmtI GCTAGC 1 cut(s) 936
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 928
BpuMI CCSGG 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 608
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse118I RCCGGY 1 cut(s) 898
Bse1I ACTGG 3 cut(s) 129, 495, 862
Bse21I CCTNAGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 1020
BseJI GATNNNNATC 1 cut(s) 66
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 3 cut(s) 252, 429, 573
BseNI ACTGG 3 cut(s) 129, 495, 862
BseRI GAGGAG 1 cut(s) 839
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 9 cut(s) 319, 415, 421, 443, 460, 473, 490, 512, 916
BseYI CCCAGC 1 cut(s) 994
BsgI GTGCAG 1 cut(s) 433
BshFI GGCC 5 cut(s) 236, 494, 902, 1049, 1059
BsiSI CCGG 4 cut(s) 525, 609, 899, 1022
BsmAI GTCTC 2 cut(s) 379, 955
BsmBI CGTCTC 1 cut(s) 379
BsnI GGCC 5 cut(s) 236, 494, 902, 1049, 1059
Bsp120I GGGCCC 1 cut(s) 1057
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp13I TCCGGA 1 cut(s) 608
Bsp143I GATC 4 cut(s) 36, 67, 77, 253
BspACI CCGC 3 cut(s) 949, 1017, 1054
BspANI GGCC 5 cut(s) 236, 494, 902, 1049, 1059
BspCNI CTCAG 3 cut(s) 251, 430, 574
BspEI TCCGGA 1 cut(s) 608
BspLI GGNNCC 6 cut(s) 235, 847, 903, 1015, 1058, 1059
BspMAI CTGCAG 1 cut(s) 433
BspOI GCTAGC 1 cut(s) 936
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 898
BsrI ACTGG 3 cut(s) 129, 495, 862
BssAI RCCGGY 1 cut(s) 898
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 4 cut(s) 36, 67, 77, 253
BssSI CACGAG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 375
Bst4CI ACNGT 4 cut(s) 403, 454, 616, 966
BstC8I GCNNGC 5 cut(s) 94, 448, 817, 921, 934
BstDEI CTNAG 4 cut(s) 238, 438, 582, 1065
BstH2I RGCGCY 1 cut(s) 993
BstHHI GCGC 1 cut(s) 992
BstKTI GATC 4 cut(s) 39, 70, 80, 256
BstMAI GTCTC 2 cut(s) 379, 955
BstMBI GATC 4 cut(s) 36, 67, 77, 253
BstMWI GCNNNNNNNGC 7 cut(s) 434, 443, 470, 500, 920, 929, 938
BstNSI RCATGY 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 1020
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 1061
BstV1I GCAGC 9 cut(s) 319, 415, 421, 443, 460, 473, 490, 512, 916
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 36, 67
BstYI RGATCY 2 cut(s) 36, 67
Bsu36I CCTNAGG 1 cut(s) 238
BsuRI GGCC 5 cut(s) 236, 494, 902, 1049, 1059
BtgZI GCGATG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 488, 870
Cac8I GCNNGC 5 cut(s) 94, 448, 817, 921, 934
CaiI CAGNNNCTG 2 cut(s) 437, 1001
CfoI GCGC 1 cut(s) 992
Cfr10I RCCGGY 1 cut(s) 898
Cfr13I GGNCC 5 cut(s) 234, 901, 1048, 1057, 1058
CseI GACGC 4 cut(s) 98, 313, 396, 737
Csp6I GTAC 1 cut(s) 372
CviAII CATG 2 cut(s) 199, 816
CviQI GTAC 1 cut(s) 372
DdeI CTNAG 4 cut(s) 238, 438, 582, 1065
DpnI GATC 4 cut(s) 38, 69, 79, 255
DpnII GATC 4 cut(s) 36, 67, 77, 253
EaeI YGGCCR 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 1061
Eco32I GATATC 1 cut(s) 727
Eco57I CTGAAG 1 cut(s) 909
Eco81I CCTNAGG 1 cut(s) 238
EcoO109I RGGNCCY 2 cut(s) 234, 1057
EcoRV GATATC 1 cut(s) 727
EcoT38I GRGCYC 1 cut(s) 1061
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 2 cut(s) 202, 819
FatI CATG 2 cut(s) 198, 815
FauI CCCGC 1 cut(s) 1047
FbaI TGATCA 1 cut(s) 253
FriOI GRGCYC 1 cut(s) 1061
FspBI CTAG 4 cut(s) 137, 521, 852, 933
GlaI GCGC 1 cut(s) 991
GsaI CCCAGC 1 cut(s) 998
GsuI CTGGAG 1 cut(s) 928
HaeII RGCGCY 1 cut(s) 993
HaeIII GGCC 5 cut(s) 236, 494, 902, 1049, 1059
HapII CCGG 4 cut(s) 525, 609, 899, 1022
HgaI GACGC 4 cut(s) 98, 313, 396, 737
HhaI GCGC 1 cut(s) 992
Hin1II CATG 2 cut(s) 202, 819
Hin6I GCGC 1 cut(s) 990
HinP1I GCGC 1 cut(s) 990
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 8 cut(s) 140, 160, 246, 538, 557, 584, 680, 827
HpaII CCGG 4 cut(s) 525, 609, 899, 1022
HphI GGTGA 1 cut(s) 1021
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 3 cut(s) 562, 583, 648
Hpy188III TCNNGA 2 cut(s) 609, 893
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 1 cut(s) 315
HpyAV CCTTC 3 cut(s) 4, 769, 805
HpyCH4III ACNGT 4 cut(s) 403, 454, 616, 966
HpyCH4IV ACGT 3 cut(s) 48, 58, 650
HpyCH4V TGCA 9 cut(s) 54, 96, 266, 322, 431, 450, 488, 500, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 434, 443, 470, 500, 920, 929, 938
HpyF3I CTNAG 4 cut(s) 238, 438, 582, 1065
HpySE526I ACGT 3 cut(s) 48, 58, 650
Hsp92II CATG 2 cut(s) 202, 819
HspAI GCGC 1 cut(s) 990
Kpn2I TCCGGA 1 cut(s) 608
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 36, 67, 77, 253
LmnI GCTCC 2 cut(s) 481, 1013
Lsp1109I GCAGC 9 cut(s) 319, 415, 421, 443, 460, 473, 490, 512, 916
MaeI CTAG 4 cut(s) 137, 521, 852, 933
MaeII ACGT 3 cut(s) 48, 58, 650
MaeIII GTNAC 2 cut(s) 194, 403
MalI GATC 4 cut(s) 38, 69, 79, 255
MboI GATC 4 cut(s) 36, 67, 77, 253
MboII GAAGA 5 cut(s) 31, 566, 728, 734, 743
MflI RGATCY 2 cut(s) 36, 67
MhlI GDGCHC 1 cut(s) 1061
MlsI TGGCCA 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 5 cut(s) 149, 255, 532, 593, 836
Mox20I TGGCCA 1 cut(s) 494
MroI TCCGGA 1 cut(s) 608
MscI TGGCCA 1 cut(s) 494
MseI TTAA 5 cut(s) 26, 168, 599, 627, 765
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 3 cut(s) 434, 503, 929
MspI CCGG 4 cut(s) 525, 609, 899, 1022
MspR9I CCNGG 1 cut(s) 1022
MwoI GCNNNNNNNGC 7 cut(s) 434, 443, 470, 500, 920, 929, 938
NciI CCSGG 1 cut(s) 1022
NdeII GATC 4 cut(s) 36, 67, 77, 253
NheI GCTAGC 1 cut(s) 932
NlaIII CATG 2 cut(s) 202, 819
NlaIV GGNNCC 6 cut(s) 235, 847, 903, 1015, 1058, 1059
NmeAIII GCCGAG 1 cut(s) 984
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 1 cut(s) 819
OliI CACNNNNGTG 1 cut(s) 455
PaeI GCATGC 1 cut(s) 819
PfeI GAWTC 3 cut(s) 160, 557, 680
PflFI GACNNNGTC 1 cut(s) 541
PleI GAGTC 5 cut(s) 148, 254, 532, 592, 835
PpsI GAGTC 5 cut(s) 148, 254, 532, 592, 835
PshBI ATTAAT 2 cut(s) 168, 765
Psp1406I AACGTT 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 994
PspN4I GGNNCC 6 cut(s) 235, 847, 903, 1015, 1058, 1059
PspOMI GGGCCC 1 cut(s) 1057
PspPI GGNCC 5 cut(s) 234, 901, 1048, 1057, 1058
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 2 cut(s) 437, 1001
PsuI RGATCY 2 cut(s) 36, 67
PsyI GACNNNGTC 1 cut(s) 541
PvuII CAGCTG 3 cut(s) 434, 503, 929
RsaI GTAC 1 cut(s) 373
RsaNI GTAC 1 cut(s) 372
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 5 cut(s) 26, 168, 599, 627, 765
Sau3AI GATC 4 cut(s) 36, 67, 77, 253
Sau96I GGNCC 5 cut(s) 234, 901, 1048, 1057, 1058
SchI GAGTC 5 cut(s) 149, 255, 532, 593, 836
ScrFI CCNGG 1 cut(s) 1022
SduI GDGCHC 1 cut(s) 1061
SfcI CTRYAG 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 455
SphI GCATGC 1 cut(s) 819
SsiI CCGC 3 cut(s) 949, 1017, 1054
SspMI CTAG 4 cut(s) 137, 521, 852, 933
StyD4I CCNGG 1 cut(s) 1020
TaaI ACNGT 4 cut(s) 403, 454, 616, 966
TaiI ACGT 3 cut(s) 51, 61, 653
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 1 cut(s) 354
TatI WGTACW 1 cut(s) 371
TauI GCSGC 2 cut(s) 951, 1019
TfiI GAWTC 3 cut(s) 160, 557, 680
Tru1I TTAA 5 cut(s) 26, 168, 599, 627, 765
Tru9I TTAA 5 cut(s) 26, 168, 599, 627, 765
TscAI CASTG 2 cut(s) 495, 877
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 9 cut(s) 307, 428, 431, 434, 461, 473, 500, 503, 929
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 5 cut(s) 23, 117, 409, 735, 749
TspRI CASTG 2 cut(s) 495, 877
Tth111I GACNNNGTC 1 cut(s) 541
VspI ATTAAT 2 cut(s) 168, 765
XapI RAATTY 3 cut(s) 273, 564, 642
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XspI CTAG 4 cut(s) 137, 521, 852, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.