Rmu_sc0003262.1_g000006

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003262.1
OG0008176
Physical Location & Seq
Reverse (-)
5335 .. 8785
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
atgcatggtgattgttacatcttcggttgggaggtccagctttcagagaaaggccttgaccagtcgcattgtcctttgtgccgacaacagattggcttcggacgctctcactgcttcatccataaagagagcgggctcataccggcggtgaccataccttcaaatcctccgcctccaccggcggttcctcgccacgtgttccctccccccgcggttcctccccctccccctccccgtcttccccgccgcgctctcggtatcccgcggccgccggcgtccccgacgcccgccaatgtcgagaaccacatccggcggtcgcgggcatcgtggcgccaatacgtggccgagcagcactaccggagggtttggtttgtgacgatggcttttgcccctgacccgggctatcaggccctcagcaaaggcttggacattatacaacagccatctgaagccgaagccgggcatcaggagtctttggagaaacgtattgctgaagaatggatgccttcttcacggaacatgacatatatgttcaagcagaaggtttctatgcttgataactctgggaagccagtccttgcatcaactgacagttcaaacccctggtgcgccctgctagaagaagtggtcagtaaagcttacggaacacttggtaagcctgagatttgtcctgcttcaacagatgctcactatttccagctacaaggcttgccagcaattggattctctcctatggccaacactcattttctgctccatgaccataacgaggtcgtggctgttggaagattccaaaatggtggtgcattcaatgttattccagattctgctgaacaaagggttgaagaggttgaacatgtggtaagggaagagaagttgagggaagcatatgagcttatcgagatatattgcgaacttattgcagcacgattgccaatgtttgagtcgcagaagaactgcctaattgatctggaggaagcagtatcaagtgtaatttttgcatctcctagatgtgcggacgtaccagaactcatggatatccacaagcatttcacagcaaaatatggaaaggagttcgtctctgcagctgttgaactgcgcccagatagtggtgtaagccgcatggtaatagaaaaattatccaccaaatcacctgatgtcaaatgggatcccgaggcatttgaagagaagcctgctgaggacctgctgaacggaccaaatacttttggcatggcttctgctacaccatcacctactcatgatgtcaggggacctactaatgtccaagttcctcctaatagtagcatggccaataaagctcatgtggaagctccccctggtccgcttccacctgataaacctcctaatgctgaagttccacgcagaaattatgagtttcaggatggacccgtgaactctaatgagcagattgcaagatcatcacctcgcttggaggatcctgcttctacaaatgtttctgccaacaaagcaaccacatctaccacatttcatccagaaacgatatcctcaggaagtgatcgaatggaaatgcatggggatgataattcttcgggcaggcagaactggaatatgcaatttaagggtactgcctctgcagcacaggcagctgctgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

545

Amino Acids

60.1

Weight (kDa)

5.72

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 277 - 388 1.4e-35 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1212
AccB1I GGYRCC 1 cut(s) 330
AccB7I CCANNNNNTGG 3 cut(s) 340, 719, 1109
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 4 cut(s) 212, 249, 265, 319
AclWI GGATC 4 cut(s) 1163, 1176, 1451, 1464
AcoI YGGCCR 4 cut(s) 266, 342, 735, 1308
AcuI CTGAAG 3 cut(s) 468, 513, 1392
AcvI CACGTG 1 cut(s) 196
AcyI GRCGYC 3 cut(s) 275, 284, 331
AfaI GTAC 2 cut(s) 1023, 1606
AfiI CCNNNNNNNGG 7 cut(s) 340, 397, 398, 459, 719, 769, 1109
AflIII ACRYGT 2 cut(s) 195, 856
AgsI TTSAA 9 cut(s) 162, 535, 597, 678, 811, 845, 854, 1094, 1184
AjnI CCWGG 2 cut(s) 602, 1336
AluBI AGCT 8 cut(s) 40, 638, 700, 895, 1088, 1319, 1331, 1628
AluI AGCT 8 cut(s) 40, 638, 700, 895, 1088, 1319, 1331, 1628
Alw26I GTCTC 1 cut(s) 1084
AlwI GGATC 4 cut(s) 1163, 1176, 1451, 1464
AlwNI CAGNNNCTG 2 cut(s) 827, 1631
Ama87I CYCGRG 2 cut(s) 397, 1172
AoxI GGCC 6 cut(s) 52, 266, 342, 408, 735, 1308
ApeKI GCWGC 6 cut(s) 349, 923, 1085, 1616, 1625, 1628
AspLEI GCGC 4 cut(s) 251, 333, 611, 1101
AspS9I GGNCC 7 cut(s) 34, 409, 1201, 1214, 1271, 1340, 1406
AsuC2I CCSGG 3 cut(s) 398, 399, 460
AsuHPI GGTGA 5 cut(s) 20, 160, 1143, 1242, 1434
AvaI CYCGRG 2 cut(s) 397, 1172
AvaII GGWCC 6 cut(s) 34, 1201, 1214, 1271, 1340, 1406
AxyI CCTNAGG 1 cut(s) 1528
BalI TGGCCA 2 cut(s) 737, 1310
BamHI GGATCC 2 cut(s) 1168, 1456
BanI GGYRCC 1 cut(s) 330
BanII GRGCYC 1 cut(s) 138
BbrPI CACGTG 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 230
BbvCI CCTCAGC 2 cut(s) 413, 1197
BbvI GCAGC 5 cut(s) 361, 935, 1097, 1615, 1628
BccI CCATC 4 cut(s) 373, 451, 1255, 1397
BcgI CGANNNNNNTGC 2 cut(s) 902, 936
BciT130I CCWGG 2 cut(s) 604, 1338
BciVI GTATCC 1 cut(s) 269
BcnI CCSGG 3 cut(s) 398, 399, 460
BcoDI GTCTC 1 cut(s) 1084
BfaI CTAG 2 cut(s) 617, 1008
BfmI CTRYAG 2 cut(s) 1083, 1614
BfoI RGCGCY 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 1212
BfuI GTATCC 1 cut(s) 269
Bme1390I CCNGG 5 cut(s) 398, 399, 460, 604, 1338
Bme18I GGWCC 6 cut(s) 34, 1201, 1214, 1271, 1340, 1406
BmeT110I CYCGRG 2 cut(s) 397, 1172
BmgT120I GGNCC 7 cut(s) 34, 409, 1201, 1214, 1271, 1340, 1406
BmiI GGNNCC 7 cut(s) 186, 216, 332, 1170, 1272, 1408, 1458
BmrFI CCNGG 5 cut(s) 398, 399, 460, 604, 1338
BmsI GCATC 6 cut(s) 332, 472, 492, 590, 673, 1010
BpiI GAAGAC 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 1064, 1096
BpmI CTGGAG 1 cut(s) 992
Bpu10I CCTNAGC 2 cut(s) 413, 1197
BpuMI CCSGG 3 cut(s) 398, 399, 460
BsaAI YACGTR 2 cut(s) 196, 340
BsaHI GRCGYC 3 cut(s) 275, 284, 331
BsaJI CCNNGG 6 cut(s) 210, 263, 397, 602, 1173, 1336
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 7 cut(s) 340, 397, 398, 459, 719, 769, 1109
Bse118I RCCGGY 3 cut(s) 142, 178, 271
Bse1I ACTGG 3 cut(s) 61, 572, 1589
Bse21I CCTNAGG 1 cut(s) 1528
BseBI CCWGG 2 cut(s) 604, 1338
BseDI CCNNGG 6 cut(s) 210, 263, 397, 602, 1173, 1336
BseGI GGATG 6 cut(s) 117, 306, 507, 1408, 1510, 1564
BseLI CCNNNNNNNGG 7 cut(s) 340, 397, 398, 459, 719, 769, 1109
BseMII CTCAG 4 cut(s) 427, 651, 1188, 1542
BseNI ACTGG 3 cut(s) 61, 572, 1589
BseX3I CGGCCG 1 cut(s) 266
BseXI GCAGC 5 cut(s) 361, 935, 1097, 1615, 1628
Bsh1236I CGCG 4 cut(s) 212, 249, 265, 319
Bsh1285I CGRYCG 2 cut(s) 269, 317
BshFI GGCC 6 cut(s) 54, 268, 344, 410, 737, 1310
BshNI GGYRCC 1 cut(s) 330
BsiEI CGRYCG 2 cut(s) 269, 317
BsiHKCI CYCGRG 2 cut(s) 397, 1172
BsiSI CCGG 7 cut(s) 143, 179, 272, 310, 358, 398, 459
BslFI GGGAC 2 cut(s) 262, 1284
BslI CCNNNNNNNGG 7 cut(s) 340, 397, 398, 459, 719, 769, 1109
BsmAI GTCTC 1 cut(s) 1084
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 262, 1284
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 6 cut(s) 54, 268, 344, 410, 737, 1310
BsoBI CYCGRG 2 cut(s) 397, 1172
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
BspANI GGCC 6 cut(s) 54, 268, 344, 410, 737, 1310
BspCNI CTCAG 4 cut(s) 426, 652, 1189, 1541
BspFNI CGCG 4 cut(s) 212, 249, 265, 319
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 7 cut(s) 186, 216, 332, 1170, 1272, 1408, 1458
BspMAI CTGCAG 2 cut(s) 1087, 1618
BspMI ACCTGC 1 cut(s) 1212
BspPI GGATC 4 cut(s) 1163, 1176, 1451, 1464
BspT107I GGYRCC 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 132
BsrFI RCCGGY 3 cut(s) 142, 178, 271
BsrI ACTGG 3 cut(s) 61, 572, 1589
BssAI RCCGGY 3 cut(s) 142, 178, 271
BssECI CCNNGG 6 cut(s) 210, 263, 397, 602, 1173, 1336
BssMI GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
BssNI GRCGYC 3 cut(s) 275, 284, 331
Bst2UI CCWGG 2 cut(s) 604, 1338
Bst4CI ACNGT 1 cut(s) 593
Bst6I CTCTTC 3 cut(s) 840, 864, 1179
BstACI GRCGYC 3 cut(s) 275, 284, 331
BstBAI YACGTR 2 cut(s) 196, 340
BstC8I GCNNGC 8 cut(s) 134, 273, 288, 321, 710, 714, 1194, 1577
BstDEI CTNAG 4 cut(s) 413, 660, 1197, 1528
BstDSI CCRYGG 2 cut(s) 210, 263
BstEII GGTNACC 1 cut(s) 148
BstF5I GGATG 6 cut(s) 117, 306, 507, 1408, 1510, 1564
BstFNI CGCG 4 cut(s) 212, 249, 265, 319
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 4 cut(s) 251, 333, 611, 1101
BstKTI GATC 5 cut(s) 970, 1171, 1439, 1459, 1540
BstMAI GTCTC 1 cut(s) 1084
BstMBI GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
BstMCI CGRYCG 2 cut(s) 269, 317
BstMWI GCNNNNNNNGC 7 cut(s) 102, 111, 1316, 1487, 1616, 1622, 1625
BstNI CCWGG 2 cut(s) 604, 1338
BstNSI RCATGY 1 cut(s) 860
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 5 cut(s) 396, 397, 458, 602, 1336
BstSFI CTRYAG 2 cut(s) 1083, 1614
BstUI CGCG 4 cut(s) 212, 249, 265, 319
BstV1I GCAGC 5 cut(s) 361, 935, 1097, 1615, 1628
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 1168, 1456
BstXI CCANNNNNNTGG 1 cut(s) 800
BstYI RGATCY 2 cut(s) 1168, 1456
BstZI CGGCCG 1 cut(s) 266
Bsu36I CCTNAGG 1 cut(s) 1528
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 6 cut(s) 54, 268, 344, 410, 737, 1310
BtgI CCRYGG 2 cut(s) 210, 263
BtsCI GGATG 6 cut(s) 117, 306, 507, 1408, 1510, 1564
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 109
BveI ACCTGC 1 cut(s) 1212
Cac8I GCNNGC 8 cut(s) 134, 273, 288, 321, 710, 714, 1194, 1577
CaiI CAGNNNCTG 2 cut(s) 827, 1631
CciI TCATGA 1 cut(s) 1258
CciNI GCGGCCGC 1 cut(s) 266
CfoI GCGC 4 cut(s) 251, 333, 611, 1101
Cfr10I RCCGGY 3 cut(s) 142, 178, 271
Cfr13I GGNCC 7 cut(s) 34, 409, 1201, 1214, 1271, 1340, 1406
Cfr42I CCGCGG 2 cut(s) 213, 266
Cfr9I CCCGGG 1 cut(s) 397
CseI GACGC 3 cut(s) 111, 264, 292
Csp6I GTAC 2 cut(s) 1022, 1605
CviQI GTAC 2 cut(s) 1022, 1605
DdeI CTNAG 4 cut(s) 413, 660, 1197, 1528
DinI GGCGCC 1 cut(s) 332
DpnI GATC 5 cut(s) 969, 1170, 1438, 1458, 1539
DpnII GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
EaeI YGGCCR 4 cut(s) 266, 342, 735, 1308
EagI CGGCCG 1 cut(s) 266
Eam1104I CTCTTC 3 cut(s) 840, 864, 1179
EarI CTCTTC 3 cut(s) 840, 864, 1179
EciI GGCGGA 1 cut(s) 159
EclXI CGGCCG 1 cut(s) 266
Eco147I AGGCCT 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 138
Eco32I GATATC 2 cut(s) 1039, 1524
Eco47I GGWCC 6 cut(s) 34, 1201, 1214, 1271, 1340, 1406
Eco52I CGGCCG 1 cut(s) 266
Eco57I CTGAAG 3 cut(s) 468, 513, 1392
Eco72I CACGTG 1 cut(s) 196
Eco81I CCTNAGG 1 cut(s) 1528
Eco88I CYCGRG 2 cut(s) 397, 1172
Eco91I GGTNACC 1 cut(s) 148
EcoO109I RGGNCCY 3 cut(s) 409, 1201, 1271
EcoO65I GGTNACC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 602, 1336
EcoRV GATATC 2 cut(s) 1039, 1524
EcoT22I ATGCAT 2 cut(s) 6, 1554
EcoT38I GRGCYC 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 332
EheI GGCGCC 1 cut(s) 332
Esp3I CGTCTC 1 cut(s) 1084
FaqI GGGAC 2 cut(s) 262, 1284
FauI CCCGC 6 cut(s) 125, 217, 251, 270, 295, 312
FauNDI CATATG 1 cut(s) 889
FokI GGATG 6 cut(s) 104, 293, 514, 1415, 1497, 1571
FriOI GRGCYC 1 cut(s) 138
FspBI CTAG 2 cut(s) 617, 1008
GlaI GCGC 4 cut(s) 250, 332, 610, 1100
GsuI CTGGAG 1 cut(s) 992
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 6 cut(s) 54, 268, 344, 410, 737, 1310
HapII CCGG 7 cut(s) 143, 179, 272, 310, 358, 398, 459
HgaI GACGC 3 cut(s) 111, 264, 292
HhaI GCGC 4 cut(s) 251, 333, 611, 1101
Hin1I GRCGYC 3 cut(s) 275, 284, 331
Hin6I GCGC 4 cut(s) 249, 331, 609, 1099
HinP1I GCGC 4 cut(s) 249, 331, 609, 1099
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 5 cut(s) 470, 723, 789, 824, 944
HpaII CCGG 7 cut(s) 143, 179, 272, 310, 358, 398, 459
HphI GGTGA 5 cut(s) 20, 160, 1143, 1242, 1434
Hpy166II GTNNAC 1 cut(s) 1414
Hpy188I TCNGA 3 cut(s) 46, 101, 448
Hpy8I GTNNAC 1 cut(s) 1414
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 3 cut(s) 168, 516, 535
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4IV ACGT 4 cut(s) 195, 339, 484, 1020
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 111, 1316, 1487, 1616, 1622, 1625
HpyF3I CTNAG 4 cut(s) 413, 660, 1197, 1528
HpySE526I ACGT 4 cut(s) 195, 339, 484, 1020
Hsp92I GRCGYC 3 cut(s) 275, 284, 331
HspAI GCGC 4 cut(s) 249, 331, 609, 1099
KasI GGCGCC 1 cut(s) 330
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
KspI CCGCGG 2 cut(s) 213, 266
Kzo9I GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
LmnI GCTCC 2 cut(s) 759, 1336
Lsp1109I GCAGC 5 cut(s) 361, 935, 1097, 1615, 1628
LweI GCATC 6 cut(s) 332, 472, 492, 590, 673, 1010
MaeI CTAG 2 cut(s) 617, 1008
MaeII ACGT 4 cut(s) 195, 339, 484, 1020
MaeIII GTNAC 3 cut(s) 14, 148, 373
MalI GATC 5 cut(s) 969, 1170, 1438, 1458, 1539
MbiI CCGCTC 1 cut(s) 132
MboI GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 1168, 1456
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 2 cut(s) 737, 1310
MluCI AATT 7 cut(s) 717, 963, 993, 1136, 1387, 1564, 1595
MluNI TGGCCA 2 cut(s) 737, 1310
Mly113I GGCGCC 1 cut(s) 331
MlyI GAGTC 2 cut(s) 479, 953
MmeI TCCRAC 1 cut(s) 763
Mox20I TGGCCA 2 cut(s) 737, 1310
Mph1103I ATGCAT 2 cut(s) 6, 1554
MreI CGCCGGCG 1 cut(s) 271
MroNI GCCGGC 1 cut(s) 271
MscI TGGCCA 2 cut(s) 737, 1310
MseI TTAA 1 cut(s) 1599
MslI CAYNNNNRTG 1 cut(s) 1557
Msp20I TGGCCA 2 cut(s) 737, 1310
MspA1I CMGCKG 4 cut(s) 212, 265, 1088, 1628
MspI CCGG 7 cut(s) 143, 179, 272, 310, 358, 398, 459
MspR9I CCNGG 5 cut(s) 398, 399, 460, 604, 1338
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 2 cut(s) 604, 1338
MvnI CGCG 4 cut(s) 212, 249, 265, 319
MwoI GCNNNNNNNGC 7 cut(s) 102, 111, 1316, 1487, 1616, 1622, 1625
NaeI GCCGGC 1 cut(s) 273
NarI GGCGCC 1 cut(s) 331
NciI CCSGG 3 cut(s) 398, 399, 460
NdeI CATATG 1 cut(s) 889
NdeII GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
NgoMIV GCCGGC 1 cut(s) 271
NlaIV GGNNCC 7 cut(s) 186, 216, 332, 1170, 1272, 1408, 1458
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 148, 373
NotI GCGGCCGC 1 cut(s) 266
NsiI ATGCAT 2 cut(s) 6, 1554
NspI RCATGY 1 cut(s) 860
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 54
PciI ACATGT 1 cut(s) 856
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 1 cut(s) 806
PdiI GCCGGC 1 cut(s) 273
PfeI GAWTC 3 cut(s) 723, 789, 824
PflMI CCANNNNNTGG 3 cut(s) 340, 719, 1109
PleI GAGTC 2 cut(s) 478, 952
PluTI GGCGCC 1 cut(s) 334
PmaCI CACGTG 1 cut(s) 196
PmlI CACGTG 1 cut(s) 196
PpsI GAGTC 2 cut(s) 478, 952
Ppu21I YACGTR 2 cut(s) 196, 340
PpuMI RGGWCCY 2 cut(s) 1201, 1271
PscI ACATGT 1 cut(s) 856
Psp5II RGGWCCY 2 cut(s) 1201, 1271
Psp6I CCWGG 2 cut(s) 602, 1336
PspCI CACGTG 1 cut(s) 196
PspEI GGTNACC 1 cut(s) 148
PspGI CCWGG 2 cut(s) 602, 1336
PspN4I GGNNCC 7 cut(s) 186, 216, 332, 1170, 1272, 1408, 1458
PspPI GGNCC 7 cut(s) 34, 409, 1201, 1214, 1271, 1340, 1406
PspPPI RGGWCCY 2 cut(s) 1201, 1271
PstI CTGCAG 2 cut(s) 1087, 1618
PstNI CAGNNNCTG 2 cut(s) 827, 1631
PsuI RGATCY 2 cut(s) 1168, 1456
PvuII CAGCTG 2 cut(s) 1088, 1628
RsaI GTAC 2 cut(s) 1023, 1606
RsaNI GTAC 2 cut(s) 1022, 1605
RseI CAYNNNNRTG 1 cut(s) 1557
SacII CCGCGG 2 cut(s) 213, 266
SaqAI TTAA 1 cut(s) 1599
Sau3AI GATC 5 cut(s) 967, 1168, 1436, 1456, 1537
Sau96I GGNCC 7 cut(s) 34, 409, 1201, 1214, 1271, 1340, 1406
SchI GAGTC 2 cut(s) 479, 953
ScrFI CCNGG 5 cut(s) 398, 399, 460, 604, 1338
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 6 cut(s) 332, 472, 492, 590, 673, 1010
SfcI CTRYAG 2 cut(s) 1083, 1614
SfoI GGCGCC 1 cut(s) 332
Sfr303I CCGCGG 2 cut(s) 213, 266
SgrAI CRCCGGYG 2 cut(s) 178, 271
SgrBI CCGCGG 2 cut(s) 213, 266
SinI GGWCC 6 cut(s) 34, 1201, 1214, 1271, 1340, 1406
SmaI CCCGGG 1 cut(s) 399
SmiMI CAYNNNNRTG 1 cut(s) 1557
Sse9I AATT 7 cut(s) 717, 963, 993, 1136, 1387, 1564, 1595
SseBI AGGCCT 1 cut(s) 54
SspDI GGCGCC 1 cut(s) 330
SspMI CTAG 2 cut(s) 617, 1008
StuI AGGCCT 1 cut(s) 54
StyD4I CCNGG 5 cut(s) 396, 397, 458, 602, 1336
TaaI ACNGT 1 cut(s) 593
TaiI ACGT 4 cut(s) 198, 342, 487, 1023
TaqI TCGA 3 cut(s) 297, 900, 1540
TasI AATT 7 cut(s) 717, 963, 993, 1136, 1387, 1564, 1595
TauI GCSGC 4 cut(s) 249, 268, 271, 1122
TfiI GAWTC 3 cut(s) 723, 789, 824
Tru1I TTAA 1 cut(s) 1599
Tru9I TTAA 1 cut(s) 1599
TscAI CASTG 1 cut(s) 116
TseFI GTSAC 2 cut(s) 148, 373
TseI GCWGC 6 cut(s) 349, 923, 1085, 1616, 1625, 1628
Tsp45I GTSAC 2 cut(s) 148, 373
TspDTI ATGAA 2 cut(s) 106, 1499
TspGWI ACGGA 3 cut(s) 529, 657, 1227
TspMI CCCGGG 1 cut(s) 397
TspRI CASTG 1 cut(s) 116
Van91I CCANNNNNTGG 3 cut(s) 340, 719, 1109
VpaK11BI GGWCC 6 cut(s) 34, 1201, 1214, 1271, 1340, 1406
XceI RCATGY 1 cut(s) 860
XmaI CCCGGG 1 cut(s) 397
XspI CTAG 2 cut(s) 617, 1008
Zsp2I ATGCAT 2 cut(s) 6, 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.