Rmu_sc0003368.1_g000013

Putative adipose-regulatory protein (Seipin)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003368.1
OG0003222
Physical Location & Seq
Reverse (-)
54517 .. 57447
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
atggaggattcaagaattctcgaagctcatgatctttctgataatggccccgaagggttctcgcggggttttgaactcgaatgtgatgttgaatcacaaaggttgttccatgtgctgaaatgttctatgctcaaattggctctggatggggctgacaaggtgttcaaggatcatagaacactgaatagtaacatatctgtacttcgatgtcgagctagatttgctggtgatcatggaggagaaaggaggtctagtctggattcaacccaagtatttgaagaaaatgttgttgaagaaagaagagatggagctataagacccccttcggagggaaacaaacctgctaactctttgctacatcatcaatcgagcaaagattgcgttaatgtatcacaagtgctccaaaaccagcttggtgtgcaaaccgtctcttttgctgctgtgattgcgtgggagtgccagcaccgtggggggattctggcatctggatttgtgctaagtgggtttgtgatgaaacacctggttgagaagcctattaagaccatagagaccctcaactttgattacaccaagccaagtccagtagctttggtgccactcatgtcttctaatgttggtgttataccttatggtcacaaattgaagatgattgtttcgttgactgtgcctgactcggaatacaatcacagacttggtgtctttcatccatccatgcatgctgcaattcaatacattcagacctttctgagaagtgctcctcttatcgctggtttacaatcagaaacccaagttctgaacatcaaaatcaatgaacacgctgaaggacttgagccaactacccgcttgaaggatactggaacaaagagcagaattccaccatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Seipin

Protein Analysis

294

Amino Acids

32.63

Weight (kDa)

6.67

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 159 - 238 2.2e-13 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 592
AccII CGCG 1 cut(s) 64
AciI CCGC 2 cut(s) 64, 841
AclWI GGATC 1 cut(s) 177
AcsI RAATTY 2 cut(s) 15, 870
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 3 cut(s) 328, 329, 847
AhdI GACNNNNNGTC 1 cut(s) 695
AjnI CCWGG 1 cut(s) 519
AleI CACNNNNGTG 1 cut(s) 880
AluBI AGCT 5 cut(s) 26, 215, 311, 412, 587
AluI AGCT 5 cut(s) 26, 215, 311, 412, 587
Alw21I GWGCWC 2 cut(s) 402, 757
Alw26I GTCTC 2 cut(s) 433, 542
AlwI GGATC 1 cut(s) 177
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 437, 719
ApoI RAATTY 2 cut(s) 15, 870
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 402, 757
BbvI GCAGC 2 cut(s) 424, 706
BccI CCATC 3 cut(s) 140, 299, 715
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 844
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 433, 542
BfaI CTAG 2 cut(s) 216, 252
BfuAI ACCTGC 1 cut(s) 349
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 2 cut(s) 438, 720
BlsI GCNGC 2 cut(s) 439, 721
Bme1390I CCNGG 1 cut(s) 521
BmeRI GACNNNNNGTC 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 49, 594
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpuEI CTTGAG 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 466, 878
BsaXI ACNNNNNCTCC 4 cut(s) 238, 268, 384, 414
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 329, 847
Bse1I ACTGG 2 cut(s) 581, 859
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 466, 878
BseGI GGATG 3 cut(s) 151, 703, 707
BseLI CCNNNNNNNGG 3 cut(s) 328, 329, 847
BseMII CTCAG 1 cut(s) 737
BseNI ACTGG 2 cut(s) 581, 859
BseRI GAGGAG 2 cut(s) 252, 747
BseXI GCAGC 2 cut(s) 424, 706
Bsh1236I CGCG 1 cut(s) 64
BshFI GGCC 1 cut(s) 48
BshNI GGYRCC 1 cut(s) 592
BsiHKAI GWGCWC 2 cut(s) 402, 757
BslI CCNNNNNNNGG 3 cut(s) 328, 329, 847
BsmAI GTCTC 2 cut(s) 433, 542
BsmBI CGTCTC 1 cut(s) 433
BsnI GGCC 1 cut(s) 48
Bso31I GGTCTC 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 402, 757
Bsp143I GATC 3 cut(s) 31, 169, 229
Bsp19I CCATGG 1 cut(s) 878
BspACI CCGC 2 cut(s) 64, 841
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 738
BspFNI CGCG 1 cut(s) 64
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 49, 594
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 542
BsrI ACTGG 2 cut(s) 581, 859
BssECI CCNNGG 2 cut(s) 466, 878
BssMI GATC 3 cut(s) 31, 169, 229
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 3 cut(s) 427, 467, 664
Bst6I CTCTTC 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 461, 717
BstDEI CTNAG 2 cut(s) 497, 746
BstDSI CCRYGG 2 cut(s) 466, 878
BstF5I GGATG 3 cut(s) 151, 703, 707
BstFNI CGCG 1 cut(s) 64
BstKTI GATC 3 cut(s) 34, 172, 232
BstMAI GTCTC 2 cut(s) 433, 542
BstMBI GATC 3 cut(s) 31, 169, 229
BstMWI GCNNNNNNNGC 4 cut(s) 221, 378, 418, 446
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 519
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 2 cut(s) 424, 706
BstV2I GAAGAC 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 467
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 466, 878
BtsCI GGATG 3 cut(s) 151, 703, 707
BtsIMutI CAGTG 1 cut(s) 179
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 2 cut(s) 461, 717
CciI TCATGA 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 519
Csp6I GTAC 1 cut(s) 200
CviAII CATG 7 cut(s) 29, 110, 233, 601, 712, 716, 879
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 2 cut(s) 497, 746
DpnI GATC 3 cut(s) 33, 171, 231
DpnII GATC 3 cut(s) 31, 169, 229
DriI GACNNNNNGTC 1 cut(s) 695
Eam1104I CTCTTC 1 cut(s) 295
Eam1105I GACNNNNNGTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 878
Eco31I GGTCTC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 840
EcoRI GAATTC 2 cut(s) 15, 870
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 1 cut(s) 878
EcoT22I ATGCAT 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 433
FaeI CATG 7 cut(s) 32, 113, 236, 604, 715, 719, 882
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FatI CATG 7 cut(s) 28, 109, 232, 600, 711, 715, 878
FauI CCCGC 2 cut(s) 57, 848
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 438, 720
FokI GGATG 3 cut(s) 158, 690, 694
Fsp4HI GCNGC 2 cut(s) 438, 720
FspBI CTAG 2 cut(s) 216, 252
GluI GCNGC 2 cut(s) 438, 720
HaeIII GGCC 1 cut(s) 48
Hin1II CATG 7 cut(s) 32, 113, 236, 604, 715, 719, 882
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HinfI GANTC 5 cut(s) 8, 92, 260, 475, 671
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 660, 773
Hpy188I TCNGA 7 cut(s) 40, 328, 676, 738, 747, 781, 795
Hpy188III TCNNGA 6 cut(s) 12, 20, 29, 143, 257, 486
Hpy8I GTNNAC 2 cut(s) 660, 773
HpyAV CCTTC 4 cut(s) 47, 333, 815, 841
HpyCH4III ACNGT 3 cut(s) 427, 467, 664
HpyCH4V TGCA 3 cut(s) 421, 715, 722
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 378, 418, 446
HpyF3I CTNAG 2 cut(s) 497, 746
Hsp92II CATG 7 cut(s) 32, 113, 236, 604, 715, 719, 882
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 3 cut(s) 31, 169, 229
LmnI GCTCC 3 cut(s) 308, 405, 760
Lsp1109I GCAGC 2 cut(s) 424, 706
LweI GCATC 1 cut(s) 491
MabI ACCWGGT 1 cut(s) 519
MaeI CTAG 2 cut(s) 216, 252
MaeIII GTNAC 2 cut(s) 188, 632
MalI GATC 3 cut(s) 33, 171, 231
MboI GATC 3 cut(s) 31, 169, 229
MboII GAAGA 5 cut(s) 290, 305, 312, 597, 655
MhlI GDGCHC 2 cut(s) 402, 757
MluCI AATT 5 cut(s) 15, 134, 638, 723, 870
MlyI GAGTC 1 cut(s) 665
MnlI CCTC 5 cut(s) 230, 240, 322, 563, 768
Mph1103I ATGCAT 1 cut(s) 717
MseI TTAA 2 cut(s) 384, 537
MslI CAYNNNNRTG 1 cut(s) 880
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 4 cut(s) 221, 378, 418, 446
NcoI CCATGG 1 cut(s) 878
NdeII GATC 3 cut(s) 31, 169, 229
NlaIII CATG 7 cut(s) 32, 113, 236, 604, 715, 719, 882
NlaIV GGNNCC 2 cut(s) 49, 594
NmuCI GTSAC 1 cut(s) 632
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 1 cut(s) 719
OliI CACNNNNGTG 1 cut(s) 880
PaeI GCATGC 1 cut(s) 719
PagI TCATGA 1 cut(s) 28
PfeI GAWTC 4 cut(s) 8, 92, 260, 475
PkrI GCNGC 2 cut(s) 439, 721
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 49, 594
PspPI GGNCC 1 cut(s) 47
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 880
SaqAI TTAA 2 cut(s) 384, 537
SatI GCNGC 2 cut(s) 438, 720
Sau3AI GATC 3 cut(s) 31, 169, 229
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 2 cut(s) 402, 757
SexAI ACCWGGT 1 cut(s) 519
SfaNI GCATC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 880
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SphI GCATGC 1 cut(s) 719
Sse9I AATT 5 cut(s) 15, 134, 638, 723, 870
SsiI CCGC 2 cut(s) 64, 841
SspMI CTAG 2 cut(s) 216, 252
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 3 cut(s) 427, 467, 664
TaqI TCGA 5 cut(s) 21, 78, 205, 211, 368
TasI AATT 5 cut(s) 15, 134, 638, 723, 870
TatI WGTACW 1 cut(s) 199
TfiI GAWTC 4 cut(s) 8, 92, 260, 475
Tru1I TTAA 2 cut(s) 384, 537
Tru9I TTAA 2 cut(s) 384, 537
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 2 cut(s) 437, 719
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 3 cut(s) 527, 692, 825
TspRI CASTG 1 cut(s) 186
XapI RAATTY 2 cut(s) 15, 870
XceI RCATGY 1 cut(s) 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XspI CTAG 2 cut(s) 216, 252
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.