Rmu_sc0003624.1_g000024

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003624.1
OG0006424
Physical Location & Seq
Forward (+)
89147 .. 89777
631 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ctgccactgtctctccctcaacgcacaggcggatctacaacctgtcatcctttccctcttctctcggttcggcgccttcaccaaacgcacgcacttgaagctctcatctcgctgcggtgccggaatctcaagcacctgaagctccgcgcgtgcctcgagttgactgacgcaggcatgctggcatccgcgaagaactgcaatggtttgaagaagctctcatgtggatcatacaccttcggagccaaaggaatgaacgccgtgttagaaaattgcttggcgctggaagagttatcagtgaagcggctccgtggtatttgggtcggacggtgtcctgacgggtggaagtcaaaccgtgtgggaaatggaggtttgatttcagtggccaagagttgccctaaccttcaggaattggttcttatcgacgtgaacctaaccaaagcgagcttggacaagttggcaacgaattgccccaatctggagaggttggcattgtgcaggagcaataatatgtttattggtggcaataatatcattattgtggggcaataa

Protein Analysis

182

Amino Acids

19.72

Weight (kDa)

9.55

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 127 - 169 6.4e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 72, 117
AccII CGCG 3 cut(s) 147, 149, 188
AciI CCGC 5 cut(s) 30, 115, 145, 186, 301
AclWI GGATC 2 cut(s) 40, 232
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 2 cut(s) 158, 386
AcyI GRCGYC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 2 cut(s) 98, 208
AjiI CACGTC 1 cut(s) 424
AluBI AGCT 4 cut(s) 101, 142, 214, 444
AluI AGCT 4 cut(s) 101, 142, 214, 444
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 2 cut(s) 40, 232
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 112
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 3 cut(s) 75, 149, 280
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 155
BalI TGGCCA 1 cut(s) 383
BanI GGYRCC 2 cut(s) 72, 117
BbvI GCAGC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 76, 281
BisI GCNGC 2 cut(s) 113, 302
BlsI GCNGC 2 cut(s) 114, 303
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 1 cut(s) 424
BmiI GGNNCC 4 cut(s) 74, 119, 241, 305
BmsI GCATC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 307
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse3DI GCAATG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 2 cut(s) 46, 182
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 1 cut(s) 205
BseXI GCAGC 1 cut(s) 99
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 3 cut(s) 147, 149, 188
BshFI GGCC 1 cut(s) 383
BshNI GGYRCC 2 cut(s) 72, 117
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 121
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 15
BsnI GGCC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 155
Bsp143I GATC 2 cut(s) 32, 224
BspACI CCGC 5 cut(s) 30, 115, 145, 186, 301
BspANI GGCC 1 cut(s) 383
BspFNI CGCG 3 cut(s) 147, 149, 188
BspLI GGNNCC 4 cut(s) 74, 119, 241, 305
BspPI GGATC 2 cut(s) 40, 232
BspT107I GGYRCC 2 cut(s) 72, 117
BsrDI GCAATG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 2 cut(s) 32, 224
BssNI GRCGYC 1 cut(s) 73
Bst4CI ACNGT 3 cut(s) 9, 327, 353
Bst6I CTCTTC 2 cut(s) 63, 279
BstACI GRCGYC 1 cut(s) 73
BstC8I GCNNGC 6 cut(s) 90, 151, 172, 176, 180, 442
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 2 cut(s) 46, 182
BstFNI CGCG 3 cut(s) 147, 149, 188
BstH2I RGCGCY 2 cut(s) 76, 281
BstHHI GCGC 3 cut(s) 75, 149, 280
BstKTI GATC 2 cut(s) 35, 227
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 2 cut(s) 32, 224
BstMWI GCNNNNNNNGC 2 cut(s) 98, 139
BstNSI RCATGY 1 cut(s) 178
BstUI CGCG 3 cut(s) 147, 149, 188
BstV1I GCAGC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 307
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 2 cut(s) 46, 182
BtsIMutI CAGTG 3 cut(s) 5, 300, 384
Cac8I GCNNGC 6 cut(s) 90, 151, 172, 176, 180, 442
CfoI GCGC 3 cut(s) 75, 149, 280
CseI GACGC 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
CviAII CATG 2 cut(s) 175, 219
CviJI RGCY 7 cut(s) 101, 142, 214, 242, 304, 383, 444
CviKI_1 RGCY 7 cut(s) 101, 142, 214, 242, 304, 383, 444
DinI GGCGCC 1 cut(s) 74
DpnI GATC 2 cut(s) 34, 226
DpnII GATC 2 cut(s) 32, 224
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 2 cut(s) 63, 279
EarI CTCTTC 2 cut(s) 63, 279
EciI GGCGGA 1 cut(s) 45
Eco57I CTGAAG 2 cut(s) 158, 386
Eco88I CYCGRG 1 cut(s) 155
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
FaeI CATG 2 cut(s) 178, 222
FaiI YATR 4 cut(s) 176, 220, 229, 509
FatI CATG 2 cut(s) 174, 218
Fnu4HI GCNGC 2 cut(s) 113, 302
FokI GGATG 2 cut(s) 33, 169
Fsp4HI GCNGC 2 cut(s) 113, 302
GlaI GCGC 3 cut(s) 74, 148, 279
GluI GCNGC 2 cut(s) 113, 302
GsuI CTGGAG 1 cut(s) 497
HaeII RGCGCY 2 cut(s) 76, 281
HaeIII GGCC 1 cut(s) 383
HapII CCGG 1 cut(s) 121
HgaI GACGC 1 cut(s) 176
HhaI GCGC 3 cut(s) 75, 149, 280
Hin1I GRCGYC 1 cut(s) 73
Hin1II CATG 2 cut(s) 178, 222
Hin6I GCGC 3 cut(s) 73, 147, 278
HinP1I GCGC 3 cut(s) 73, 147, 278
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 1 cut(s) 124
HpaII CCGG 1 cut(s) 121
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 162, 427
Hpy188I TCNGA 2 cut(s) 239, 323
Hpy188III TCNNGA 3 cut(s) 332, 404, 476
Hpy8I GTNNAC 2 cut(s) 162, 427
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 3 cut(s) 86, 244, 410
HpyCH4III ACNGT 3 cut(s) 9, 327, 353
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 2 cut(s) 198, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 139
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 73
Hsp92II CATG 2 cut(s) 178, 222
HspAI GCGC 3 cut(s) 73, 147, 278
KasI GGCGCC 1 cut(s) 72
Kzo9I GATC 2 cut(s) 32, 224
LmnI GCTCC 4 cut(s) 147, 239, 309, 498
Lsp1109I GCAGC 1 cut(s) 99
LweI GCATC 1 cut(s) 191
MaeII ACGT 1 cut(s) 423
MalI GATC 2 cut(s) 34, 226
MboI GATC 2 cut(s) 32, 224
MboII GAAGA 4 cut(s) 50, 202, 220, 296
MflI RGATCY 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 3 cut(s) 268, 407, 463
MluNI TGGCCA 1 cut(s) 383
Mly113I GGCGCC 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 5 cut(s) 27, 66, 164, 359, 474
Mox20I TGGCCA 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 1 cut(s) 121
MvnI CGCG 3 cut(s) 147, 149, 188
MwoI GCNNNNNNNGC 2 cut(s) 98, 139
NarI GGCGCC 1 cut(s) 73
NdeII GATC 2 cut(s) 32, 224
NlaIII CATG 2 cut(s) 178, 222
NlaIV GGNNCC 4 cut(s) 74, 119, 241, 305
NspI RCATGY 1 cut(s) 178
PaeI GCATGC 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 327
PkrI GCNGC 2 cut(s) 114, 303
PluTI GGCGCC 1 cut(s) 76
PspN4I GGNNCC 4 cut(s) 74, 119, 241, 305
PspXI VCTCGAGB 1 cut(s) 155
PsuI RGATCY 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 327
RseI CAYNNNNRTG 1 cut(s) 536
SatI GCNGC 2 cut(s) 113, 302
Sau3AI GATC 2 cut(s) 32, 224
SfaNI GCATC 1 cut(s) 191
SfoI GGCGCC 1 cut(s) 74
Sfr274I CTCGAG 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 128, 155
SmoI CTYRAG 2 cut(s) 128, 155
SphI GCATGC 1 cut(s) 178
Sse9I AATT 3 cut(s) 268, 407, 463
SsiI CCGC 5 cut(s) 30, 115, 145, 186, 301
SspDI GGCGCC 1 cut(s) 72
TaaI ACNGT 3 cut(s) 9, 327, 353
TaiI ACGT 1 cut(s) 426
TaqI TCGA 2 cut(s) 156, 420
TasI AATT 3 cut(s) 268, 407, 463
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 1 cut(s) 124
TscAI CASTG 3 cut(s) 12, 300, 384
TseI GCWGC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 3 cut(s) 12, 300, 384
Tth111I GACNNNGTC 1 cut(s) 327
XceI RCATGY 1 cut(s) 178
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XhoI CTCGAG 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.