Rmu_sc0004065.1_g000007

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004065.1
OG0001909
Physical Location & Seq
Reverse (-)
31983 .. 42888
10906 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4902 bp
atgcatttgccagaggaagaaggccagattcccctgaaacgcagcctcactgaaaccactgatgatcatggtgccgccgccgatcaaggagtttcaaagcgcctcaatcccagaaggtatcagacacaagtgtatgaggttgctaggaggaggaacacaatagctgttatggagacaggttctgggaagacaatgatggctgtgatgctcattgatgaaattgtggaggagtattacggagccaagggggttgatgcgtgggctaaggagcattgggaaaaggaatttaaaacccatgatgtactggttatgacaccccagattcttttggatgccttaagaaaggcattcttgaccatagaagcagtatgcttgatgattatggatgagtgccatcggactaccggaaaccacccttataaaaagataatgaaggagttttatcacaggtctagtatcaagccaaaagtttttggaatgacggcttctcctgtaattcgaaagggtgtctcatccacaatggactgtgaagatcaaatatctgaacttgaaagcattttggattcccagatttatactatagaggataaaacagagatggaagtatatgcccctactgcaaaagaaactcgtagattttataacccaacatggttttcgagtttggatttgaaagaaaagatcaaggcctcttggtcgaagaatgatgcttcatttttaaagctgcaagaatcaattcaaagcaatttcaaagatttggatgacaaagttaaggccctgcggaaacgattgtctaatgactatgaaaagatcctgtattgcctggacgaccttggtctcatatgtgcttatgaggctgtgaaggtctgtctagagaatgctcctactagcgaagagtgtgaattttatagagaaagttctttgcagtgcagatattttcttgaggaagtttttggtataatcaagcaatctctttcaaatggttgtgaaaattttttggatgttgggtttgactatttgaaggcagtagatttgggctacatatctccaaagttaaatgaacttgttcagcttttccattcgtttggagtatctagggaactactgtgcctcatctttgtggatagaattgtcactgctaaagtgattgaaagatttatgagaaaagttacctccttatctcatttcaaaatttcctatttgactgggagtactacatcagttgatgctctgacaccaaaagtacaaaaggagacattgaagttattcagctctggggaggtcaatttattattttctaccgatgtagttgaggagggaattcatgttccaaactgctcctgtgtgatacgttttgacttgccaaagacagtgcgtagttatgtccagtccagaggacgggctcgtcaagataactctcaattcattataatgctagaaaggggaaacaagcaacagagggatcaactatatgagatcatcaagagcgaaggtttgatgacaaatacagctctaaatagagatcctgaagaaagcgttttgaaaccatgtacacttgatgaaataagtgaatatgttgtgaatgccaccggagcatcagttacagcagattccagtgtccgtactatacataagtattgtgacacgcttcccagagacaagtactttgttccaagaccagcatttcagttttcatatctcggagattcttatgaatgtaagattacattgcctccaaatgcggctttccaaaccttggtcggtcctgcatgcaaaaactccgctttatcaaagcaggttgtatgcttagaagcttgtaagaagctgcatcaattgggtgccttgaatgatcatcttctcccgtccactgataagcctttggagaaagatataaatgtagaaagcaaagggccagcttctggtgcaggaacaactaaaaggaaggaactacatgggacaactggcattcgcgctctgtcaggaacttgggcagaaaaactggatggtgccgttttttacgcctataaatttgacttctcctgtgatattgttaccgagctatattctggatttattcttctgattgagtcagaccttgctaaggatgtggggaatattgaattgacactttacttggtttcaaagaaagttaaagcttctgtttcttcctgtgggcaagttcgtttggatggagaacagatggcaaaagcaaagctctttcaggaatttttcttcaatggattgtttgggaaattgtttcatcgagtcaacccagctgaaaagcagagagagtttttacttatgaaagaaacaagaaagctgtggaggcaatcatacatgtatttggttcttccgcttgaaacattggatgattcaaataatgaatcttggacaataaattggggagggatcagttcttgtgtttctgtggtagagttcttgaagcaaaatgcattgttgggtgctcagcattgtaagggtgatgcaaggaattcatcgcaaagcagaagtggcgcatctgggactggatgcaatagttcacagatattccactttgctgattcttctgttaatgaaaaaaatctcaaagatatggtggtagtggccatccacaccggaaaaatatattctgtttttgaggttttgagtgacacatccgctgagagtccttttgacacatcacagtacaatacctatgctgagtacttccacaaaaaatatggggttctgctcatgtatccgggtcagcctctgttgcggctgaagcaaaatcataatccacacaaccttcttgtgaacttcaatggcgaaggtggtagtggtaagacatcagaatctggccttgttaatggaaaggaacgtatgtatgcccatatgcctcctgagcttctagtctgtactggattgcgaagagacgttttgaagtcattttatctactaccatcattgattcatcgattggaatccttgatgttagccagccaactcagagaagagattgacagtcactgcagcttacagatatcaagctcattgattctagaagcacttacatcactcagatgcagtgaagatttttccatggagcgtttggaattgcttggtgattcagtgctaaagtacacagtaggctgccacctcttcctaaaatatcccaaaaaacatgatggacaattatcttctctgcgccagtctgtaatttgcaatgctaacctgcataaattgggaatagatcgaaacttacagggatatatacgtgatggtgcttttgatccacgccgttgggttggtccaggacaggtctctatacgacctgttccttgtgagtgtggagtggataccttagaagttccagtagatagcaagtttcaaactagagaccgtgaagttgtggttggaaagtgctgtgacaagggccatagatggatgggttccaaaacaatctcggattgcgttgaagcccttataggagcttactatgtagctggtggattatctgctgcagtccattttatgaagtggctccaaattgatgtagaacttgagccatccttggtttctgaagcaattactactgcatcacttcattcctgcaacccaaaggtggatgagattggaatcctagagtcaaagcttcgttatgtattttctaccaagggtctcttacaggaagctataacacatgcatctgagcaagaatcaaaacttggctattcttatgagagactagagtttctcggtgattgtgtgttggacctgcttatcacatggtatctctatcagaaccacaaagatattgatccaggggagttaactgacttgcgtgccgcttcagttaacaatgaaaactttgctcaagctgctgtgagacacaaccttcaacagcatctacaacattgctctgggttacttcaaagtcaaataacagaatatgtaaagttgctttctgaacatgatagtggtgacaaacttcaatggccaaaaggtcctaaggctcttggagacatggtggaaagtattgcaggggcaatactaattgatacggagctcaaccttgatgaagtgtggagagtatacaaaccactgttatctccaattgtgacccctgataaacttgagctgcctcccttgcgtgaactgattgaattatgtggctcacttgggtacttttataaagaacaaactgtaaagaagggtgaaatagttcacgcagagcttagtgtacagctgaaagatgtcctgttgattggagaggggattgatcggagtaagaaaatcgcaaaaggagaagcagctcatcatttgttgaaggagcttgagaaaagaggtattacacactctgggtctgccaggaagagaaaactgggtgctgaccaagttgatgattcatctccaataaagcaaaagaaaactgagatgcatctgcccatggatgcaactggactttctttgccagccactgacagaaaaataagtggttcgaaaaatgacatcccagttatcaaagcaatcaacatgaagaaaggaggacctcgagtctctctttacgagctctgcaaacaactacaatggcaaatgcctaccttcaaaccaacagaaaaggattccagtttcatatcaacaataacgttgtacatacccaatttgggcaacatcgaatgcacaggagatcctaggtcagataagaagagttcactagactctgcagcacttctcatgctttatgatctcgaaaggcaggactcagaccaatatcaggcaatcaatatccggattgaccttgggtccccattttaccacatcaattcaacaattggcaacggacacgtggctcgtaaacaacagcccctacgtggatatgccacgtggtcggggacacggggcatcgtgaataagcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1633

Amino Acids

183.52

Weight (kDa)

6.51

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 352 - 468 2.7e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 539 - 625 1.8e-23 Dicer dimerisation domain
PAZ PF02170 855 - 982 2.4e-11 PAZ domain
Ribonucleas_3_3 PF14622 1027 - 1086 5.2e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1029 - 1164 2.4e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1220 - 1347 1.4e-13 Ribonuclease-III-like
Ribonuclease_3 PF00636 1241 - 1348 1.9e-19 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 420, 642, 1430, 4173
AasI GACNNNNNNGTC 1 cut(s) 1404
Acc36I ACCTGC 3 cut(s) 1786, 3222, 3765
AccB1I GGYRCC 3 cut(s) 71, 1838, 2006
AccB7I CCANNNNNTGG 2 cut(s) 1756, 1919
AccI GTMKAC 1 cut(s) 4074
AccII CGCG 1 cut(s) 1971
AccIII TCCGGA 1 cut(s) 4770
AciI CCGC 9 cut(s) 75, 78, 781, 1742, 1782, 2354, 2658, 2758, 3828
AclI AACGTT 1 cut(s) 4628
AclWI GGATC 7 cut(s) 805, 1470, 1517, 2417, 3266, 3794, 4664
AcoI YGGCCR 2 cut(s) 2604, 3977
AcsI RAATTY 8 cut(s) 284, 902, 991, 1191, 1320, 2027, 2225, 2491
AcuI CTGAAG 4 cut(s) 1548, 2783, 3582, 3816
AcvI CACGTG 2 cut(s) 4828, 4866
AfiI CCNNNNNNNGG 8 cut(s) 1398, 1756, 1919, 2101, 3604, 4646, 4756, 4853
AflII CTTAAG 1 cut(s) 337
AflIII ACRYGT 2 cut(s) 2337, 4825
AhdI GACNNNNNGTC 2 cut(s) 4535, 4783
AjnI CCWGG 4 cut(s) 822, 3292, 3802, 4349
AjuI GAANNNNNNNTTGG 6 cut(s) 640, 672, 3378, 3410, 3690, 3722
AloI GAACNNNNNNTCC 2 cut(s) 1311, 1343
Alw21I GWGCWC 3 cut(s) 2467, 4050, 4554
AlwI GGATC 7 cut(s) 805, 1470, 1517, 2417, 3266, 3794, 4664
AlwNI CAGNNNCTG 6 cut(s) 182, 1919, 2752, 2837, 3009, 4460
Ama87I CYCGRG 1 cut(s) 4533
Aor13HI TCCGGA 1 cut(s) 4770
AoxI GGCC 8 cut(s) 22, 687, 774, 1910, 2604, 2839, 3415, 3977
ApoI RAATTY 8 cut(s) 284, 902, 991, 1191, 1320, 2027, 2225, 2491
ArsI GACNNNNNNTTYG 2 cut(s) 1649, 1681
Asp700I GAANNNNTTC 4 cut(s) 735, 1065, 1265, 4203
AspA2I CCTAGG 1 cut(s) 4673
AspLEI GCGC 4 cut(s) 102, 1973, 2516, 3189
AspS9I GGNCC 9 cut(s) 775, 1763, 1910, 3290, 3415, 3754, 3986, 4529, 4785
AsuC2I CCSGG 1 cut(s) 2742
AsuHPI GGTGA 5 cut(s) 2492, 3116, 3752, 3974, 4208
AsuII TTCGAA 2 cut(s) 499, 4481
AvaI CYCGRG 1 cut(s) 4533
AvaII GGWCC 6 cut(s) 1763, 3290, 3754, 3986, 4529, 4785
AvrII CCTAGG 1 cut(s) 4673
AxyI CCTNAGG 1 cut(s) 3990
BalI TGGCCA 2 cut(s) 2606, 3979
BanI GGYRCC 3 cut(s) 71, 1838, 2006
BanII GRGCYC 3 cut(s) 1405, 4050, 4554
BbrPI CACGTG 2 cut(s) 4828, 4866
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 3 cut(s) 2467, 4050, 4554
BceAI ACGGC 3 cut(s) 498, 1994, 3264
BciT130I CCWGG 4 cut(s) 824, 3294, 3804, 4351
BciVI GTATCC 2 cut(s) 2748, 3331
BclI TGATCA 2 cut(s) 64, 1849
BcnI CCSGG 1 cut(s) 2742
BfmI CTRYAG 4 cut(s) 579, 3010, 3501, 4704
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 337
BfuAI ACCTGC 3 cut(s) 1786, 3222, 3765
BfuI GTATCC 2 cut(s) 2748, 3331
BlnI CCTAGG 1 cut(s) 4673
BlpI GCTNAGC 1 cut(s) 2466
BmcAI AGTACT 3 cut(s) 1213, 1664, 2705
Bme1390I CCNGG 5 cut(s) 824, 2742, 3294, 3804, 4351
Bme18I GGWCC 6 cut(s) 1763, 3290, 3754, 3986, 4529, 4785
BmeRI GACNNNNNGTC 2 cut(s) 4535, 4783
BmeT110I CYCGRG 1 cut(s) 4533
BmgT120I GGNCC 9 cut(s) 775, 1763, 1910, 3290, 3415, 3754, 3986, 4529, 4785
BmiI GGNNCC 8 cut(s) 73, 241, 1840, 2008, 3433, 3524, 4786, 4787
BmrFI CCNGG 5 cut(s) 824, 2742, 3294, 3804, 4351
BmrI ACTGGG 3 cut(s) 1215, 4373, 4490
BmuI ACTGGG 3 cut(s) 1215, 4373, 4490
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 194
Bpu10I CCTNAGC 3 cut(s) 264, 2100, 2883
Bpu1102I GCTNAGC 1 cut(s) 2466
Bpu14I TTCGAA 2 cut(s) 499, 4481
BpuEI CTTGAG 5 cut(s) 962, 3563, 3840, 4136, 4337
BpuMI CCSGG 1 cut(s) 2742
Bsa29I ATCGAT 1 cut(s) 2956
BsaAI YACGTR 4 cut(s) 3257, 4828, 4853, 4866
BsaBI GATNNNNATC 3 cut(s) 2962, 3617, 3798
BsaI GGTCTC 4 cut(s) 842, 3307, 3372, 3665
BsaWI WCCGGW 4 cut(s) 404, 1589, 2615, 4770
BsaXI ACNNNNNCTCC 8 cut(s) 472, 502, 1584, 1614, 1717, 1747, 3993, 4023
Bsc4I CCNNNNNNNGG 8 cut(s) 1398, 1756, 1919, 2101, 3604, 4646, 4756, 4853
Bse21I CCTNAGG 1 cut(s) 3990
Bse3DI GCAATG 3 cut(s) 1727, 3211, 3895
Bse8I GATNNNNATC 3 cut(s) 2962, 3617, 3798
BseAI TCCGGA 1 cut(s) 4770
BseBI CCWGG 4 cut(s) 824, 3294, 3804, 4351
BseCI ATCGAT 1 cut(s) 2956
BseJI GATNNNNATC 3 cut(s) 2962, 3617, 3798
BseLI CCNNNNNNNGG 8 cut(s) 1398, 1756, 1919, 2101, 3604, 4646, 4756, 4853
BseMI GCAATG 3 cut(s) 1727, 3211, 3895
BseMII CTCAG 9 cut(s) 2480, 2652, 2691, 2874, 3001, 3073, 3681, 4404, 4758
BseRI GAGGAG 3 cut(s) 163, 242, 1328
BseYI CCCAGC 1 cut(s) 2272
BsgI GTGCAG 2 cut(s) 949, 1944
Bsh1236I CGCG 1 cut(s) 1971
BshFI GGCC 8 cut(s) 24, 689, 776, 1912, 2606, 2841, 3417, 3979
BshNI GGYRCC 3 cut(s) 71, 1838, 2006
BshVI ATCGAT 1 cut(s) 2956
BsiHKAI GWGCWC 3 cut(s) 2467, 4050, 4554
BsiHKCI CYCGRG 1 cut(s) 4533
BsiSI CCGG 5 cut(s) 405, 1590, 2616, 2741, 4771
BslFI GGGAC 4 cut(s) 1969, 2536, 4771, 4888
BslI CCNNNNNNNGG 8 cut(s) 1398, 1756, 1919, 2101, 3604, 4646, 4756, 4853
BsmBI CGTCTC 1 cut(s) 2907
BsmFI GGGAC 4 cut(s) 1969, 2536, 4771, 4888
BsmI GAATGC 5 cut(s) 347, 883, 1588, 1965, 4664
BsnI GGCC 8 cut(s) 24, 689, 776, 1912, 2606, 2841, 3417, 3979
Bso31I GGTCTC 4 cut(s) 842, 3307, 3372, 3665
BsoBI CYCGRG 1 cut(s) 4533
Bsp119I TTCGAA 2 cut(s) 499, 4481
Bsp1286I GDGCHC 4 cut(s) 1405, 2467, 4050, 4554
Bsp13I TCCGGA 1 cut(s) 4770
Bsp1407I TGTACA 3 cut(s) 1550, 4222, 4632
Bsp1720I GCTNAGC 1 cut(s) 2466
Bsp19I CCATGG 2 cut(s) 3081, 4428
BspACI CCGC 9 cut(s) 75, 78, 781, 1742, 1782, 2354, 2658, 2758, 3828
BspANI GGCC 8 cut(s) 24, 689, 776, 1912, 2606, 2841, 3417, 3979
BspCNI CTCAG 9 cut(s) 2479, 2653, 2692, 2875, 3000, 3072, 3682, 4405, 4757
BspDI ATCGAT 1 cut(s) 2956
BspEI TCCGGA 1 cut(s) 4770
BspFNI CGCG 1 cut(s) 1971
BspLI GGNNCC 8 cut(s) 73, 241, 1840, 2008, 3433, 3524, 4786, 4787
BspMAI CTGCAG 3 cut(s) 3014, 3505, 4708
BspMI ACCTGC 3 cut(s) 1786, 3222, 3765
BspPI GGATC 7 cut(s) 805, 1470, 1517, 2417, 3266, 3794, 4664
BspT104I TTCGAA 2 cut(s) 499, 4481
BspT107I GGYRCC 3 cut(s) 71, 1838, 2006
BspTI CTTAAG 1 cut(s) 337
BspTNI GGTCTC 4 cut(s) 842, 3307, 3372, 3665
BsrDI GCAATG 3 cut(s) 1727, 3211, 3895
BsrGI TGTACA 3 cut(s) 1550, 4222, 4632
BssNAI GTATAC 1 cut(s) 4075
BssT1I CCWWGG 9 cut(s) 243, 832, 1755, 3081, 3552, 3654, 4428, 4673, 4780
Bst1107I GTATAC 1 cut(s) 4075
Bst2UI CCWGG 4 cut(s) 824, 3294, 3804, 4351
Bst4CI ACNGT 9 cut(s) 527, 1107, 1372, 2685, 3005, 3127, 3383, 4086, 4186
Bst6I CTCTTC 6 cut(s) 888, 2905, 2988, 3146, 4349, 4682
BstAFI CTTAAG 1 cut(s) 337
BstAUI TGTACA 3 cut(s) 1550, 4222, 4632
BstBAI YACGTR 4 cut(s) 3257, 4828, 4853, 4866
BstBI TTCGAA 2 cut(s) 499, 4481
BstC8I GCNNGC 5 cut(s) 1771, 1914, 2980, 3825, 4455
BstDSI CCRYGG 2 cut(s) 3081, 4428
BstENI CCTNNNNNAGG 1 cut(s) 2099
BstFNI CGCG 1 cut(s) 1971
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 4 cut(s) 102, 1973, 2516, 3189
BstNI CCWGG 4 cut(s) 824, 3294, 3804, 4351
BstNSI RCATGY 3 cut(s) 1773, 2341, 3686
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 5 cut(s) 822, 2740, 3292, 3802, 4349
BstSFI CTRYAG 4 cut(s) 579, 3010, 3501, 4704
BstUI CGCG 1 cut(s) 1971
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 3 cut(s) 810, 1522, 4669
BstXI CCANNNNNNTGG 2 cut(s) 1085, 3282
BstYI RGATCY 3 cut(s) 810, 1522, 4669
BstZ17I GTATAC 1 cut(s) 4075
Bsu15I ATCGAT 1 cut(s) 2956
Bsu36I CCTNAGG 1 cut(s) 3990
BsuI GTATCC 2 cut(s) 2748, 3331
BsuRI GGCC 8 cut(s) 24, 689, 776, 1912, 2606, 2841, 3417, 3979
BsuTUI ATCGAT 1 cut(s) 2956
BtgI CCRYGG 2 cut(s) 3081, 4428
BtgZI GCGATG 1 cut(s) 2481
BtsI GCAGTG 4 cut(s) 932, 1134, 3007, 3073
BveI ACCTGC 3 cut(s) 1786, 3222, 3765
Cac8I GCNNGC 5 cut(s) 1771, 1914, 2980, 3825, 4455
CaiI CAGNNNCTG 6 cut(s) 182, 1919, 2752, 2837, 3009, 4460
CfoI GCGC 4 cut(s) 102, 1973, 2516, 3189
Cfr13I GGNCC 9 cut(s) 775, 1763, 1910, 3290, 3415, 3754, 3986, 4529, 4785
ClaI ATCGAT 1 cut(s) 2956
DraI TTTAAA 2 cut(s) 289, 720
DrdI GACNNNNNNGTC 1 cut(s) 1404
DriI GACNNNNNGTC 2 cut(s) 4535, 4783
DseDI GACNNNNNNGTC 1 cut(s) 1404
EaeI YGGCCR 2 cut(s) 2604, 3977
Eam1104I CTCTTC 6 cut(s) 888, 2905, 2988, 3146, 4349, 4682
Eam1105I GACNNNNNGTC 2 cut(s) 4535, 4783
EarI CTCTTC 6 cut(s) 888, 2905, 2988, 3146, 4349, 4682
Ecl136II GAGCTC 2 cut(s) 4048, 4552
Eco130I CCWWGG 9 cut(s) 243, 832, 1755, 3081, 3552, 3654, 4428, 4673, 4780
Eco147I AGGCCT 1 cut(s) 689
Eco24I GRGCYC 3 cut(s) 1405, 4050, 4554
Eco31I GGTCTC 4 cut(s) 842, 3307, 3372, 3665
Eco32I GATATC 1 cut(s) 3024
Eco47I GGWCC 6 cut(s) 1763, 3290, 3754, 3986, 4529, 4785
Eco53kI GAGCTC 2 cut(s) 4048, 4552
Eco57I CTGAAG 4 cut(s) 1548, 2783, 3582, 3816
Eco72I CACGTG 2 cut(s) 4828, 4866
Eco81I CCTNAGG 1 cut(s) 3990
Eco88I CYCGRG 1 cut(s) 4533
EcoICRI GAGCTC 2 cut(s) 4048, 4552
EcoNI CCTNNNNNAGG 1 cut(s) 2099
EcoO109I RGGNCCY 4 cut(s) 775, 3986, 4529, 4785
EcoRI GAATTC 2 cut(s) 1320, 2491
EcoRII CCWGG 4 cut(s) 822, 3292, 3802, 4349
EcoRV GATATC 1 cut(s) 3024
EcoT14I CCWWGG 9 cut(s) 243, 832, 1755, 3081, 3552, 3654, 4428, 4673, 4780
EcoT22I ATGCAT 4 cut(s) 6, 2455, 3688, 4422
EcoT38I GRGCYC 3 cut(s) 1405, 4050, 4554
ErhI CCWWGG 9 cut(s) 243, 832, 1755, 3081, 3552, 3654, 4428, 4673, 4780
Esp3I CGTCTC 1 cut(s) 2907
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 3647, 3679
FaqI GGGAC 4 cut(s) 1969, 2536, 4771, 4888
FauNDI CATATG 2 cut(s) 842, 2874
FbaI TGATCA 2 cut(s) 64, 1849
FblI GTMKAC 1 cut(s) 4074
FriOI GRGCYC 3 cut(s) 1405, 4050, 4554
GlaI GCGC 4 cut(s) 101, 1972, 2515, 3188
GsaI CCCAGC 1 cut(s) 2276
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 8 cut(s) 24, 689, 776, 1912, 2606, 2841, 3417, 3979
HapII CCGG 5 cut(s) 405, 1590, 2616, 2741, 4771
HhaI GCGC 4 cut(s) 102, 1973, 2516, 3189
Hin6I GCGC 4 cut(s) 100, 1971, 2514, 3187
HinP1I GCGC 4 cut(s) 100, 1971, 2514, 3187
HincII GTYRAC 3 cut(s) 2269, 3813, 3838
HindII GTYRAC 3 cut(s) 2269, 3813, 3838
HindIII AAGCTT 3 cut(s) 1812, 2154, 3632
HpaI GTTAAC 2 cut(s) 3813, 3838
HpaII CCGG 5 cut(s) 405, 1590, 2616, 2741, 4771
HphI GGTGA 5 cut(s) 2492, 3116, 3752, 3974, 4208
HpyCH4III ACNGT 9 cut(s) 527, 1107, 1372, 2685, 3005, 3127, 3383, 4086, 4186
HpyCH4IV ACGT 8 cut(s) 1351, 2860, 2916, 3256, 4628, 4827, 4852, 4865
HpySE526I ACGT 8 cut(s) 1351, 2860, 2916, 3256, 4628, 4827, 4852, 4865
HspAI GCGC 4 cut(s) 100, 1971, 2514, 3187
KflI GGGWCCC 1 cut(s) 4785
Kpn2I TCCGGA 1 cut(s) 4770
Ksp22I TGATCA 2 cut(s) 64, 1849
KspAI GTTAAC 2 cut(s) 3813, 3838
MaeII ACGT 8 cut(s) 1351, 2860, 2916, 3256, 4628, 4827, 4852, 4865
MfeI CAATTG 3 cut(s) 1832, 4095, 4812
MflI RGATCY 3 cut(s) 810, 1522, 4669
MhlI GDGCHC 4 cut(s) 1405, 2467, 4050, 4554
MlsI TGGCCA 2 cut(s) 2606, 3979
MluNI TGGCCA 2 cut(s) 2606, 3979
MlyI GAGTC 7 cut(s) 2096, 2274, 2674, 3635, 4545, 4694, 4736
MmeI TCCRAC 2 cut(s) 3376, 3732
Mox20I TGGCCA 2 cut(s) 2606, 3979
Mph1103I ATGCAT 4 cut(s) 6, 2455, 3688, 4422
MroI TCCGGA 1 cut(s) 4770
MroXI GAANNNNTTC 4 cut(s) 735, 1065, 1265, 4203
MscI TGGCCA 2 cut(s) 2606, 3979
MslI CAYNNNNRTG 4 cut(s) 1118, 1430, 3061, 3957
Msp20I TGGCCA 2 cut(s) 2606, 3979
MspA1I CMGCKG 3 cut(s) 2276, 2660, 4228
MspCI CTTAAG 1 cut(s) 337
MspI CCGG 5 cut(s) 405, 1590, 2616, 2741, 4771
MspR9I CCNGG 5 cut(s) 824, 2742, 3294, 3804, 4351
MunI CAATTG 3 cut(s) 1832, 4095, 4812
Mva1269I GAATGC 5 cut(s) 347, 883, 1588, 1965, 4664
MvaI CCWGG 4 cut(s) 824, 3294, 3804, 4351
MvnI CGCG 1 cut(s) 1971
NciI CCSGG 1 cut(s) 2742
NcoI CCATGG 2 cut(s) 3081, 4428
NdeI CATATG 2 cut(s) 842, 2874
NlaIV GGNNCC 8 cut(s) 73, 241, 1840, 2008, 3433, 3524, 4786, 4787
NmuCI GTSAC 7 cut(s) 1132, 1640, 2648, 3005, 3407, 3962, 4099
NsiI ATGCAT 4 cut(s) 6, 2455, 3688, 4422
NspI RCATGY 3 cut(s) 1773, 2341, 3686
NspV TTCGAA 2 cut(s) 499, 4481
PaeI GCATGC 1 cut(s) 1773
PaeR7I CTCGAG 1 cut(s) 4533
PceI AGGCCT 1 cut(s) 689
PciI ACATGT 1 cut(s) 2337
PctI GAATGC 5 cut(s) 347, 883, 1588, 1965, 4664
PdmI GAANNNNTTC 4 cut(s) 735, 1065, 1265, 4203
PflMI CCANNNNNTGG 2 cut(s) 1756, 1919
PfoI TCCNGGA 1 cut(s) 3292
PleI GAGTC 7 cut(s) 2095, 2273, 2673, 3634, 4544, 4694, 4736
PmaCI CACGTG 2 cut(s) 4828, 4866
PmlI CACGTG 2 cut(s) 4828, 4866
PpsI GAGTC 7 cut(s) 2095, 2273, 2673, 3634, 4544, 4694, 4736
Ppu21I YACGTR 4 cut(s) 3257, 4828, 4853, 4866
PpuMI RGGWCCY 3 cut(s) 3986, 4529, 4785
PscI ACATGT 1 cut(s) 2337
PshAI GACNNNNGTC 1 cut(s) 834
PsiI TTATAA 4 cut(s) 420, 642, 1430, 4173
Psp124BI GAGCTC 2 cut(s) 4050, 4554
Psp1406I AACGTT 1 cut(s) 4628
Psp5II RGGWCCY 3 cut(s) 3986, 4529, 4785
Psp6I CCWGG 4 cut(s) 822, 3292, 3802, 4349
PspCI CACGTG 2 cut(s) 4828, 4866
PspFI CCCAGC 1 cut(s) 2272
PspGI CCWGG 4 cut(s) 822, 3292, 3802, 4349
PspN4I GGNNCC 8 cut(s) 73, 241, 1840, 2008, 3433, 3524, 4786, 4787
PspPI GGNCC 9 cut(s) 775, 1763, 1910, 3290, 3415, 3754, 3986, 4529, 4785
PspPPI RGGWCCY 3 cut(s) 3986, 4529, 4785
PspXI VCTCGAGB 1 cut(s) 4533
PstI CTGCAG 3 cut(s) 3014, 3505, 4708
PstNI CAGNNNCTG 6 cut(s) 182, 1919, 2752, 2837, 3009, 4460
PsuI RGATCY 3 cut(s) 810, 1522, 4669
PvuII CAGCTG 2 cut(s) 2276, 4228
RseI CAYNNNNRTG 4 cut(s) 1118, 1430, 3061, 3957
SacI GAGCTC 2 cut(s) 4050, 4554
Sau96I GGNCC 9 cut(s) 775, 1763, 1910, 3290, 3415, 3754, 3986, 4529, 4785
ScaI AGTACT 3 cut(s) 1213, 1664, 2705
SchI GAGTC 7 cut(s) 2096, 2274, 2674, 3635, 4545, 4694, 4736
ScrFI CCNGG 5 cut(s) 824, 2742, 3294, 3804, 4351
SduI GDGCHC 4 cut(s) 1405, 2467, 4050, 4554
SfcI CTRYAG 4 cut(s) 579, 3010, 3501, 4704
Sfr274I CTCGAG 1 cut(s) 4533
SfuI TTCGAA 2 cut(s) 499, 4481
SinI GGWCC 6 cut(s) 1763, 3290, 3754, 3986, 4529, 4785
SlaI CTCGAG 1 cut(s) 4533
SmiMI CAYNNNNRTG 4 cut(s) 1118, 1430, 3061, 3957
SmlI CTYRAG 7 cut(s) 337, 941, 3542, 3855, 4115, 4316, 4533
SmoI CTYRAG 7 cut(s) 337, 941, 3542, 3855, 4115, 4316, 4533
SphI GCATGC 1 cut(s) 1773
SseBI AGGCCT 1 cut(s) 689
SsiI CCGC 9 cut(s) 75, 78, 781, 1742, 1782, 2354, 2658, 2758, 3828
SspI AATATT 1 cut(s) 2116
SstI GAGCTC 2 cut(s) 4050, 4554
StuI AGGCCT 1 cut(s) 689
StyD4I CCNGG 5 cut(s) 822, 2740, 3292, 3802, 4349
StyI CCWWGG 9 cut(s) 243, 832, 1755, 3081, 3552, 3654, 4428, 4673, 4780
TaaI ACNGT 9 cut(s) 527, 1107, 1372, 2685, 3005, 3127, 3383, 4086, 4186
TaiI ACGT 8 cut(s) 1354, 2863, 2919, 3259, 4631, 4830, 4855, 4868
TaqII GACCGA 1 cut(s) 1751
TauI GCSGC 5 cut(s) 77, 80, 1745, 2761, 3830
TseFI GTSAC 7 cut(s) 1132, 1640, 2648, 3005, 3407, 3962, 4099
Tsp45I GTSAC 7 cut(s) 1132, 1640, 2648, 3005, 3407, 3962, 4099
TspGWI ACGGA 4 cut(s) 252, 1610, 4058, 4836
Van91I CCANNNNNTGG 2 cut(s) 1756, 1919
Vha464I CTTAAG 1 cut(s) 337
VpaK11BI GGWCC 6 cut(s) 1763, 3290, 3754, 3986, 4529, 4785
XagI CCTNNNNNAGG 1 cut(s) 2099
XapI RAATTY 8 cut(s) 284, 902, 991, 1191, 1320, 2027, 2225, 2491
XbaI TCTAGA 2 cut(s) 871, 3040
XceI RCATGY 3 cut(s) 1773, 2341, 3686
XcmI CCANNNNNNNNNTGG 2 cut(s) 2717, 3088
XhoI CTCGAG 1 cut(s) 4533
XmaJI CCTAGG 1 cut(s) 4673
XmiI GTMKAC 1 cut(s) 4074
XmnI GAANNNNTTC 4 cut(s) 735, 1065, 1265, 4203
ZrmI AGTACT 3 cut(s) 1213, 1664, 2705
Zsp2I ATGCAT 4 cut(s) 6, 2455, 3688, 4422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.