Rmu_sc0004135.1_g000023

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004135.1
OG0003157
Physical Location & Seq
Reverse (-)
122972 .. 124902
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
atgggtcgtgacggcgtggtctggacaatgttagtcgtggcggcatggtccgaacgatgtcaaggcagtgagtcgaggcagaagcgcattagaggcttctggtacggcggtggttgctgggtagattcaatgagctccaacacaactgagacgaagaaggagaagaagggtcctcctcaaattgttaaattgaataaagctttggctttggccgaaaaatgggtgagtaacatgagtaaactgccagcagaagatgagtcttttgagacacagagtcgacctgctaggcttggactaggtgctaaagttccccgtgcatccacatttgtgccttcagatgatccccttgaaagaaagctatattacaagttgaatgctcaaaagagaaaagctgccaaaattgcagaggagttagcaccagctgctgcagatggtgatgatgacgatgaagataatgaagacagcagaacccgtgcgtttgagaagaaaaagagagcagctaatccactgagcccacctttagggaaaaagaaaaggagaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

181

Amino Acids

20.24

Weight (kDa)

9.69

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 60 - 179 2.7e-12 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 277
AciI CCGC 2 cut(s) 41, 108
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 219, 521
AgsI TTSAA 4 cut(s) 129, 193, 350, 373
AhdI GACNNNNNGTC 1 cut(s) 273
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 6 cut(s) 135, 200, 358, 392, 422, 500
AluI AGCT 6 cut(s) 135, 200, 358, 392, 422, 500
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 2 cut(s) 143, 260
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 4 cut(s) 392, 422, 425, 497
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 48, 170
AsuHPI GGTGA 2 cut(s) 235, 446
AvaII GGWCC 2 cut(s) 48, 170
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BanII GRGCYC 2 cut(s) 137, 515
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 4 cut(s) 379, 409, 412, 509
BccI CCATC 1 cut(s) 425
BceAI ACGGC 2 cut(s) 28, 121
BcoDI GTCTC 2 cut(s) 143, 260
BfaI CTAG 2 cut(s) 285, 296
BfmI CTRYAG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 5 cut(s) 42, 393, 423, 426, 498
BlsI GCNGC 5 cut(s) 43, 394, 424, 427, 499
Bme18I GGWCC 2 cut(s) 48, 170
BmeRI GACNNNNNGTC 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 48, 170
BmiI GGNNCC 1 cut(s) 171
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 465
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 521
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 219, 521
BseMII CTCAG 2 cut(s) 138, 500
BseRI GAGGAG 2 cut(s) 165, 422
BseXI GCAGC 4 cut(s) 379, 409, 412, 509
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 219, 521
BsmAI GTCTC 2 cut(s) 143, 260
BsmBI CGTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 137, 515
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 2 cut(s) 41, 108
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 139, 501
BspLI GGNNCC 1 cut(s) 171
BspMAI CTGCAG 1 cut(s) 430
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 335
BssMI GATC 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 147, 509
BstF5I GGATG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 143, 260
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 93, 114, 401, 422
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 379, 409, 412, 509
BstV2I GAAGAC 1 cut(s) 465
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 317
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 506
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 246
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 48, 170
Csp6I GTAC 1 cut(s) 103
CviAII CATG 2 cut(s) 45, 232
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 147, 509
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
DrdI GACNNNNNNGTC 1 cut(s) 17
DriI GACNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 135
Eco24I GRGCYC 2 cut(s) 137, 515
Eco47I GGWCC 2 cut(s) 48, 170
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 170
EcoT38I GRGCYC 2 cut(s) 137, 515
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 2 cut(s) 48, 235
FaiI YATR 3 cut(s) 46, 233, 361
FatI CATG 2 cut(s) 44, 231
FblI GTMKAC 1 cut(s) 277
Fnu4HI GCNGC 5 cut(s) 42, 393, 423, 426, 498
FokI GGATG 1 cut(s) 304
FriOI GRGCYC 2 cut(s) 137, 515
Fsp4HI GCNGC 5 cut(s) 42, 393, 423, 426, 498
FspBI CTAG 2 cut(s) 285, 296
GlaI GCGC 1 cut(s) 86
GluI GCNGC 5 cut(s) 42, 393, 423, 426, 498
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 1 cut(s) 212
HhaI GCGC 1 cut(s) 87
Hin1II CATG 2 cut(s) 48, 235
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 1 cut(s) 278
HindII GTYRAC 1 cut(s) 278
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 4 cut(s) 71, 125, 257, 274
HphI GGTGA 2 cut(s) 235, 446
Hpy166II GTNNAC 2 cut(s) 239, 278
Hpy188I TCNGA 2 cut(s) 52, 337
Hpy188III TCNNGA 2 cut(s) 8, 22
Hpy8I GTNNAC 2 cut(s) 239, 278
HpyAV CCTTC 3 cut(s) 151, 160, 342
HpyCH4V TGCA 3 cut(s) 317, 404, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 114, 401, 422
HpyF3I CTNAG 2 cut(s) 147, 509
Hsp92II CATG 2 cut(s) 48, 235
HspAI GCGC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 6 cut(s) 7, 85, 103, 258, 294, 432
Lsp1109I GCAGC 4 cut(s) 379, 409, 412, 509
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 285, 296
MaeIII GTNAC 2 cut(s) 8, 227
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 6 cut(s) 166, 175, 263, 461, 470, 496
MhlI GDGCHC 2 cut(s) 137, 515
MluCI AATT 3 cut(s) 180, 188, 399
MlyI GAGTC 3 cut(s) 80, 266, 283
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 5 cut(s) 69, 86, 183, 186, 400
MseI TTAA 1 cut(s) 186
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 379
MwoI GCNNNNNNNGC 4 cut(s) 93, 114, 401, 422
NdeII GATC 1 cut(s) 340
NlaIII CATG 2 cut(s) 48, 235
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 326
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 125
PkrI GCNGC 5 cut(s) 43, 394, 424, 427, 499
PleI GAGTC 3 cut(s) 79, 265, 282
PpsI GAGTC 3 cut(s) 79, 265, 282
PpuMI RGGWCCY 1 cut(s) 170
Psp124BI GAGCTC 1 cut(s) 137
Psp5II RGGWCCY 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 2 cut(s) 48, 170
PspPPI RGGWCCY 1 cut(s) 170
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 137
SalI GTCGAC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 5 cut(s) 42, 393, 423, 426, 498
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 2 cut(s) 48, 170
SchI GAGTC 3 cut(s) 80, 266, 283
SduI GDGCHC 2 cut(s) 137, 515
SetI ASST 9 cut(s) 137, 202, 283, 301, 360, 394, 424, 502, 520
SfaNI GCATC 1 cut(s) 326
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 48, 170
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 3 cut(s) 180, 188, 399
SsiI CCGC 2 cut(s) 41, 108
SspMI CTAG 2 cut(s) 285, 296
SstI GAGCTC 1 cut(s) 137
TaqI TCGA 2 cut(s) 74, 277
TasI AATT 3 cut(s) 180, 188, 399
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 2 cut(s) 73, 513
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 4 cut(s) 392, 422, 425, 497
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 2 cut(s) 462, 471
TspRI CASTG 2 cut(s) 73, 513
VpaK11BI GGWCC 2 cut(s) 48, 170
XmiI GTMKAC 1 cut(s) 277
XspI CTAG 2 cut(s) 285, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.