Rmu_sc0004464.1_g000008

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004464.1
OG0003891
Physical Location & Seq
Forward (+)
23114 .. 31106
7993 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3156 bp
atgtggacggcgaccggtgacctgaatccagcgaaagttggccgaattccggcagccggtgacgggactccggctgccgatgacagagctccgaaaaaagaagcaataaaaactgatacaagggtttgcatagaagtatacaataacctacatatcaagtcatcaccatccttgcctggaatcttgggcgtgttcttattcttaaaggcaatggaagggacgagttttgcagcagaatctcagccaaaagaggttcattcttctaacaacaaccagagaagtgacaacatagttgagtgctctggcagtcctctaagtcgaaagatatcagctagatgggatccagatgaagcatgccggcctgtcattgatgatgcacctgtattttatccaactgccgaggagttcgaagatacacttggttacatagcaaagatttggccacaggctgaatcttatggtatttgtcggattgtccctccacgttcctggatccctccttgccctcttaaagagaaagacatgtgggaaaatgctaaattctctactcgtgttcagcaagttgacttgcttcaaaatagagagtccatgaaaaagaaaactagaggtcggaagcgaaaacgaagaagacactcaaggagacctgcagaagctaatgctgcttctgagaccgatgagaagtttgggttccagtcaggatcggactttacatttgcagagtttcagagacaagcctctactttcaaggaaagttactttggaacacatgaatgtaaggagggttcaaattctggtggaactaacaacaaaagatgggaaccttctgtggaggatattgaaggtgaatactggcgaatagtagagcaaccaacagacgaggttgaggtttactatggagctgatttggaaacaggagtatttggaagtgggtttcccaaggcatcgtctgtggtaactaggagtgagtcagatcattatgcaatgtcaggctggaatctaaataacttgcctcgcttgcctggttctgtactatgttttgaggaaagcgatatctcgggagttctagtcccatggctctatgttgggatgtgcttttcttcgttttgttggcatgttgaggaccaccacctctattccctaaattatctacacttcggtgacccaaaggtatggtatggagtgcctggaagccatgctacctctttggaacaagcaatgagaaagcatttgccggatttgtttgaggaacaacctgatttactcaatgaactggtcactcagctatctccttcagttttaaagtctgagggtgtacctgtgcatcgagctatccaacattctggggagtttgttcttacctttccgagggcatatcactctggatttaattgtggcttcaattgtgctgaggcagtaaatgtcgcccctgttgactggttgcaacatggccaaactgcagtcgagctttacagtaagcagtgtcgcaagacatcactgtcacacgacaaattgttactggggtcagctctggaagccgtgcaggtcctcggggaaatatcactcggaacaaaatttattaccaaccggagctggcaaaaagtttgtggcaaagatggtgtgctcaccaaagcagttaagatggaaaaagaatttgattcaaacactgagagagaatgcttttcttgctcctatgacttgcacttatctgctgccagctgcaactgctctcctgaccgattttcatgtcttaaacatgcaaagaatttctgttcatgtgaaatgacccagagatatgtccttctccgctacagtattgaggaattaaatttgctggttaaagcattggaaggggagttggatgccattcacgtatgggcatctaaggattctggagtagtttctatagactacactcgtaagtgcactgctaagaagcctaagctggatcaagattccaagtcttgcgatccaatggaaattttgcctggttgcgcagtttcagaagataaggttaacatgaatggatcttgcagttcctctagtcctgtttcttctgcagtagttcagtcaggatcacaggatggtcataatgaagatcaaactttggccgtgaatgctgaagcaaaggtggagcatgattgcagttttgatttgaatctcaattgtatgtctgatgaacatgaaagcaagataattgacgtatctgacggctgtgataataaggctagtacagttgaggaagagacatccacatcaatgtccaatcaagagaagactagcacgtccgagggtaataagttgtttggtgttgatcttggcttatcacgtcctgcttcaaacataccaccaatcagttcttcacaaactgagattggcgatacctcagctgtgaatgcatccatgggagaccaaagctatcgattaagatgttcgagtgttgttgagcctttgaatttcggatctgtcatggctgggaaatactggtgcaccaagccggccatatatccaaaagggtttagaagccgagttaatttctacagtgtgctggacccaacaaaactgtgtagttatatatcagaagttctcgatgctgggcttcttggacccctatttaaggttagtttagaagaattcccagatgaggcttttgcaaatgtatcagcggacaagtgctgggagatggtactacatagactaaacaatgaaataagtagacggagtagtctagacgaaggagggccccctttacagtctttgcagagcattaacgggtttgcaatgtttggctttctctcccaacctattgttgaggctattgaggcccttgatcctgatcatctatgcacagagtactggaatcacaggtgtacgcagcagcattcatctgtaccaagttgctctctaggagcgccgcagacaaagctatttggtatgaacatgacaaacaaggagcataatcaaccatctgtggagggtcatcactcaatcgatgagatgcagttagttttacgaagactcttagagaaggcgaatccaaatcccaaagagttgaggacattacatagaatgttatccagtcagtcagccgaagcaagagtggcatgcgcagaattgattgaagagatgcagagaaatttagataacagtaagtcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1051

Amino Acids

116.75

Weight (kDa)

5.45

Isoelectric Point (pI)

53.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 127 - 160 8.5e-16 jmjN domain
JmjC PF02373 358 - 474 2.8e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 559 - 609 2e-14 C5HC2 zinc finger
FYRN PF05964 808 - 863 1.9e-11 F/Y-rich N-terminus
FYRC PF05965 868 - 955 9.9e-21 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1495, 2281, 2721
Acc16I TGCGCA 2 cut(s) 1993, 3106
Acc36I ACCTGC 2 cut(s) 652, 1531
AccI GTMKAC 2 cut(s) 138, 2713
AciI CCGC 3 cut(s) 1804, 2663, 2912
AcoI YGGCCR 5 cut(s) 40, 440, 1447, 2106, 2496
AcuI CTGAAG 2 cut(s) 1275, 2139
AfaI GTAC 7 cut(s) 1029, 1314, 2230, 2685, 2852, 2869, 2889
AfiI CCNNNNNNNGG 6 cut(s) 49, 56, 63, 1365, 2614, 2641
AflIII ACRYGT 1 cut(s) 522
AgeI ACCGGT 1 cut(s) 14
AjiI CACGTC 2 cut(s) 2283, 2327
AjnI CCWGG 5 cut(s) 175, 488, 1018, 1183, 1984
AjuI GAANNNNNNNTTGG 6 cut(s) 402, 434, 741, 773, 2341, 2373
AleI CACNNNNGTG 1 cut(s) 1155
Alw21I GWGCWC 5 cut(s) 91, 302, 1623, 1925, 2489
Alw26I GTCTC 5 cut(s) 634, 662, 721, 2237, 2400
Alw44I GTGCAC 2 cut(s) 1921, 2485
Ama87I CYCGRG 2 cut(s) 1054, 1547
AoxI GGCC 8 cut(s) 40, 359, 440, 1447, 2106, 2496, 2738, 2820
ApaI GGGCCC 1 cut(s) 2742
ApaLI GTGCAC 2 cut(s) 1921, 2485
ApeKI GCWGC 8 cut(s) 53, 74, 230, 659, 1709, 1716, 2872, 2875
ArsI GACNNNNNNTTYG 2 cut(s) 695, 727
AsiGI ACCGGT 1 cut(s) 14
AspLEI GCGC 3 cut(s) 1994, 2911, 3107
AspS9I GGNCC 7 cut(s) 1120, 1543, 2548, 2603, 2738, 2739, 2821
AsuHPI GGTGA 6 cut(s) 29, 71, 156, 854, 1169, 1615
AsuII TTCGAA 1 cut(s) 408
AvaI CYCGRG 2 cut(s) 1054, 1547
AvaII GGWCC 4 cut(s) 1120, 1543, 2548, 2603
BaeGI GKGCMC 3 cut(s) 1925, 2489, 2742
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BalI TGGCCA 2 cut(s) 442, 1449
BamHI GGATCC 2 cut(s) 340, 492
BanII GRGCYC 2 cut(s) 91, 2742
BarI GAAGNNNNNNTAC 2 cut(s) 1180, 1212
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 3 cut(s) 634, 2279, 3019
Bbv12I GWGCWC 5 cut(s) 91, 302, 1623, 1925, 2489
BbvCI CCTCAGC 2 cut(s) 1407, 2383
BbvI GCAGC 8 cut(s) 61, 65, 242, 646, 1696, 1703, 2884, 2887
BccI CCATC 8 cut(s) 175, 330, 807, 1607, 1633, 2075, 2674, 2971
BceAI ACGGC 4 cut(s) 24, 1520, 2093, 2224
BciT130I CCWGG 5 cut(s) 177, 490, 1020, 1185, 1986
BclI TGATCA 1 cut(s) 2833
BcoDI GTCTC 5 cut(s) 634, 662, 721, 2237, 2400
BfaI CTAG 9 cut(s) 333, 603, 957, 1064, 2040, 2226, 2277, 2726, 2903
BfmI CTRYAG 6 cut(s) 645, 1455, 1807, 1902, 2055, 2536
BfoI RGCGCY 1 cut(s) 2912
BfuAI ACCTGC 2 cut(s) 652, 1531
BisI GCNGC 9 cut(s) 54, 75, 231, 660, 1710, 1717, 2873, 2876, 2912
BlsI GCNGC 9 cut(s) 55, 76, 232, 661, 1711, 1718, 2874, 2877, 2913
BmcAI AGTACT 1 cut(s) 2852
Bme1390I CCNGG 5 cut(s) 177, 490, 1020, 1185, 1986
Bme18I GGWCC 4 cut(s) 1120, 1543, 2548, 2603
BmeT110I CYCGRG 2 cut(s) 1054, 1547
BmgBI CACGTC 2 cut(s) 2283, 2327
BmgT120I GGNCC 7 cut(s) 1120, 1543, 2548, 2603, 2738, 2739, 2821
BmiI GGNNCC 8 cut(s) 342, 494, 689, 819, 2550, 2605, 2740, 2741
BmrFI CCNGG 5 cut(s) 177, 490, 1020, 1185, 1986
BmrI ACTGGG 1 cut(s) 1526
BmsI GCATC 9 cut(s) 364, 950, 1330, 1849, 1886, 2405, 2578, 2985, 3114
BmuI ACTGGG 1 cut(s) 1526
BpiI GAAGAC 3 cut(s) 634, 2279, 3019
BpmI CTGGAG 1 cut(s) 1911
Bpu10I CCTNAGC 3 cut(s) 1407, 1938, 2383
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 619
Bsa29I ATCGAT 2 cut(s) 2419, 2988
BsaAI YACGTR 1 cut(s) 1870
BsaBI GATNNNNATC 2 cut(s) 2154, 2832
BsaI GGTCTC 3 cut(s) 634, 662, 2400
BsaJI CCNNGG 7 cut(s) 399, 936, 1070, 1364, 1546, 2286, 2400
BsaWI WCCGGW 2 cut(s) 14, 1584
BsaXI ACNNNNNCTCC 2 cut(s) 1050, 1080
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 56, 63, 1365, 2614, 2641
Bse118I RCCGGY 4 cut(s) 14, 56, 357, 2494
Bse1I ACTGG 8 cut(s) 691, 854, 1275, 1439, 1521, 2486, 2858, 3075
Bse3DI GCAATG 4 cut(s) 216, 987, 1221, 2784
Bse8I GATNNNNATC 2 cut(s) 2154, 2832
BseBI CCWGG 5 cut(s) 177, 490, 1020, 1185, 1986
BseCI ATCGAT 2 cut(s) 2419, 2988
BseDI CCNNGG 7 cut(s) 399, 936, 1070, 1364, 1546, 2286, 2400
BseGI GGATG 6 cut(s) 167, 1092, 1864, 2086, 2246, 2396
BseJI GATNNNNATC 2 cut(s) 2154, 2832
BseLI CCNNNNNNNGG 6 cut(s) 49, 56, 63, 1365, 2614, 2641
BseMI GCAATG 4 cut(s) 216, 987, 1221, 2784
BseMII CTCAG 8 cut(s) 254, 657, 1292, 1296, 1398, 1656, 2358, 2397
BseNI ACTGG 8 cut(s) 691, 854, 1275, 1439, 1521, 2486, 2858, 3075
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 3 cut(s) 1925, 2489, 2742
BseXI GCAGC 8 cut(s) 61, 65, 242, 646, 1696, 1703, 2884, 2887
BseYI CCCAGC 3 cut(s) 2471, 2591, 2673
BsgI GTGCAG 1 cut(s) 1559
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 8 cut(s) 42, 361, 442, 1449, 2108, 2498, 2740, 2822
BshTI ACCGGT 1 cut(s) 14
BshVI ATCGAT 2 cut(s) 2419, 2988
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 5 cut(s) 91, 302, 1623, 1925, 2489
BsiHKCI CYCGRG 2 cut(s) 1054, 1547
BsiSI CCGG 8 cut(s) 15, 50, 57, 71, 358, 1232, 1585, 2495
BslFI GGGAC 4 cut(s) 79, 232, 461, 1052
BslI CCNNNNNNNGG 6 cut(s) 49, 56, 63, 1365, 2614, 2641
BsmAI GTCTC 5 cut(s) 634, 662, 721, 2237, 2400
BsmFI GGGAC 4 cut(s) 79, 232, 461, 1052
BsmI GAATGC 4 cut(s) 1679, 2119, 2398, 2878
BsnI GGCC 8 cut(s) 42, 361, 442, 1449, 2108, 2498, 2740, 2822
Bso31I GGTCTC 3 cut(s) 634, 662, 2400
BsoBI CYCGRG 2 cut(s) 1054, 1547
Bsp119I TTCGAA 1 cut(s) 408
Bsp120I GGGCCC 1 cut(s) 2738
Bsp1286I GDGCHC 6 cut(s) 91, 302, 1623, 1925, 2489, 2742
Bsp19I CCATGG 2 cut(s) 1070, 2400
BspACI CCGC 3 cut(s) 1804, 2663, 2912
BspANI GGCC 8 cut(s) 42, 361, 442, 1449, 2108, 2498, 2740, 2822
BspCNI CTCAG 8 cut(s) 253, 658, 1291, 1297, 1399, 1657, 2359, 2396
BspDI ATCGAT 2 cut(s) 2419, 2988
BspLI GGNNCC 8 cut(s) 342, 494, 689, 819, 2550, 2605, 2740, 2741
BspMAI CTGCAG 3 cut(s) 649, 1459, 2059
BspMI ACCTGC 2 cut(s) 652, 1531
BspT104I TTCGAA 1 cut(s) 408
BspTNI GGTCTC 3 cut(s) 634, 662, 2400
BsrDI GCAATG 4 cut(s) 216, 987, 1221, 2784
BsrFI RCCGGY 4 cut(s) 14, 56, 357, 2494
BsrI ACTGG 8 cut(s) 691, 854, 1275, 1439, 1521, 2486, 2858, 3075
BssAI RCCGGY 4 cut(s) 14, 56, 357, 2494
BssECI CCNNGG 7 cut(s) 399, 936, 1070, 1364, 1546, 2286, 2400
BssNAI GTATAC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 3 cut(s) 936, 1070, 2400
Bst1107I GTATAC 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 5 cut(s) 177, 490, 1020, 1185, 1986
Bst4CI ACNGT 8 cut(s) 1472, 1497, 1811, 2233, 2540, 2562, 2751, 3146
Bst6I CTCTTC 2 cut(s) 2235, 3114
BstBAI YACGTR 1 cut(s) 1870
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 7 cut(s) 355, 359, 1016, 1592, 1714, 2496, 3103
BstDSI CCRYGG 2 cut(s) 1070, 2400
BstEII GGTNACC 2 cut(s) 17, 1157
BstENI CCTNNNNNAGG 2 cut(s) 1363, 2612
BstF5I GGATG 6 cut(s) 167, 1092, 1864, 2086, 2246, 2396
BstH2I RGCGCY 1 cut(s) 2912
BstHHI GCGC 3 cut(s) 1994, 2911, 3107
BstMAI GTCTC 5 cut(s) 634, 662, 721, 2237, 2400
BstMCI CGRYCG 1 cut(s) 15
BstNI CCWGG 5 cut(s) 177, 490, 1020, 1185, 1986
BstNSI RCATGY 5 cut(s) 357, 526, 1115, 1757, 3105
BstPI GGTNACC 2 cut(s) 17, 1157
BstSCI CCNGG 5 cut(s) 175, 488, 1018, 1183, 1984
BstSFI CTRYAG 6 cut(s) 645, 1455, 1807, 1902, 2055, 2536
BstSLI GKGCMC 3 cut(s) 1925, 2489, 2742
BstV1I GCAGC 8 cut(s) 61, 65, 242, 646, 1696, 1703, 2884, 2887
BstV2I GAAGAC 3 cut(s) 634, 2279, 3019
BstX2I RGATCY 4 cut(s) 340, 492, 2024, 2459
BstXI CCANNNNNNTGG 3 cut(s) 489, 1170, 1340
BstYI RGATCY 4 cut(s) 340, 492, 2024, 2459
BstZ17I GTATAC 1 cut(s) 139
Bsu15I ATCGAT 2 cut(s) 2419, 2988
BsuRI GGCC 8 cut(s) 42, 361, 442, 1449, 2108, 2498, 2740, 2822
BsuTUI ATCGAT 2 cut(s) 2419, 2988
BtgI CCRYGG 2 cut(s) 1070, 2400
BtrI CACGTC 2 cut(s) 2283, 2327
BtsCI GGATG 6 cut(s) 167, 1092, 1864, 2086, 2246, 2396
BtsI GCAGTG 2 cut(s) 1484, 1923
BtsIMutI CAGTG 5 cut(s) 1484, 1493, 1662, 1923, 2545
BveI ACCTGC 2 cut(s) 652, 1531
Cac8I GCNNGC 7 cut(s) 355, 359, 1016, 1592, 1714, 2496, 3103
CfoI GCGC 3 cut(s) 1994, 2911, 3107
Cfr10I RCCGGY 4 cut(s) 14, 56, 357, 2494
Cfr13I GGNCC 7 cut(s) 1120, 1543, 2548, 2603, 2738, 2739, 2821
ClaI ATCGAT 2 cut(s) 2419, 2988
Csp6I GTAC 7 cut(s) 1028, 1313, 2229, 2684, 2851, 2868, 2888
CspAI ACCGGT 1 cut(s) 14
CviQI GTAC 7 cut(s) 1028, 1313, 2229, 2684, 2851, 2868, 2888
DraI TTTAAA 1 cut(s) 1299
DrdI GACNNNNNNGTC 3 cut(s) 1495, 2281, 2721
DseDI GACNNNNNNGTC 3 cut(s) 1495, 2281, 2721
EaeI YGGCCR 5 cut(s) 40, 440, 1447, 2106, 2496
Eam1104I CTCTTC 2 cut(s) 2235, 3114
EarI CTCTTC 2 cut(s) 2235, 3114
Ecl136II GAGCTC 1 cut(s) 89
Eco130I CCWWGG 3 cut(s) 936, 1070, 2400
Eco24I GRGCYC 2 cut(s) 91, 2742
Eco31I GGTCTC 3 cut(s) 634, 662, 2400
Eco32I GATATC 2 cut(s) 327, 1051
Eco47I GGWCC 4 cut(s) 1120, 1543, 2548, 2603
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 1275, 2139
Eco88I CYCGRG 2 cut(s) 1054, 1547
Eco91I GGTNACC 2 cut(s) 17, 1157
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 2 cut(s) 1363, 2612
EcoO109I RGGNCCY 4 cut(s) 1543, 2738, 2739, 2821
EcoO65I GGTNACC 2 cut(s) 17, 1157
EcoRI GAATTC 2 cut(s) 45, 2630
EcoRII CCWGG 5 cut(s) 175, 488, 1018, 1183, 1984
EcoRV GATATC 2 cut(s) 327, 1051
EcoT14I CCWWGG 3 cut(s) 936, 1070, 2400
EcoT22I ATGCAT 1 cut(s) 2398
EcoT38I GRGCYC 2 cut(s) 91, 2742
ErhI CCWWGG 3 cut(s) 936, 1070, 2400
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 4 cut(s) 79, 232, 461, 1052
FbaI TGATCA 1 cut(s) 2833
FblI GTMKAC 2 cut(s) 138, 2713
Fnu4HI GCNGC 9 cut(s) 54, 75, 231, 660, 1710, 1717, 2873, 2876, 2912
FokI GGATG 6 cut(s) 154, 1099, 1871, 2093, 2233, 2383
FriOI GRGCYC 2 cut(s) 91, 2742
Fsp4HI GCNGC 9 cut(s) 54, 75, 231, 660, 1710, 1717, 2873, 2876, 2912
FspBI CTAG 9 cut(s) 333, 603, 957, 1064, 2040, 2226, 2277, 2726, 2903
FspI TGCGCA 2 cut(s) 1993, 3106
GlaI GCGC 3 cut(s) 1993, 2910, 3106
GluI GCNGC 9 cut(s) 54, 75, 231, 660, 1710, 1717, 2873, 2876, 2912
GsaI CCCAGC 3 cut(s) 2475, 2595, 2677
GsuI CTGGAG 1 cut(s) 1911
HaeII RGCGCY 1 cut(s) 2912
HaeIII GGCC 8 cut(s) 42, 361, 442, 1449, 2108, 2498, 2740, 2822
HapII CCGG 8 cut(s) 15, 50, 57, 71, 358, 1232, 1585, 2495
HhaI GCGC 3 cut(s) 1994, 2911, 3107
Hin6I GCGC 3 cut(s) 1992, 2909, 3105
HinP1I GCGC 3 cut(s) 1992, 2909, 3105
HincII GTYRAC 3 cut(s) 565, 1432, 2014
HindII GTYRAC 3 cut(s) 565, 1432, 2014
HpaI GTTAAC 1 cut(s) 2014
HpaII CCGG 8 cut(s) 15, 50, 57, 71, 358, 1232, 1585, 2495
HphI GGTGA 6 cut(s) 29, 71, 156, 854, 1169, 1615
HpyAV CCTTC 8 cut(s) 209, 831, 833, 1299, 1808, 1841, 2726, 3019
HpyCH4III ACNGT 8 cut(s) 1472, 1497, 1811, 2233, 2540, 2562, 2751, 3146
HpyCH4IV ACGT 5 cut(s) 484, 1869, 2199, 2282, 2326
HpySE526I ACGT 5 cut(s) 484, 1869, 2199, 2282, 2326
HspAI GCGC 3 cut(s) 1992, 2909, 3105
KroI GCCGGC 2 cut(s) 357, 2494
KroNI GCCGGC 2 cut(s) 359, 2496
Ksp22I TGATCA 1 cut(s) 2833
KspAI GTTAAC 1 cut(s) 2014
LmnI GCTCC 7 cut(s) 94, 896, 1587, 1691, 2131, 2906, 2950
Lsp1109I GCAGC 8 cut(s) 61, 65, 242, 646, 1696, 1703, 2884, 2887
LweI GCATC 9 cut(s) 364, 950, 1330, 1849, 1886, 2405, 2578, 2985, 3114
MaeI CTAG 9 cut(s) 333, 603, 957, 1064, 2040, 2226, 2277, 2726, 2903
MaeII ACGT 5 cut(s) 484, 1869, 2199, 2282, 2326
MfeI CAATTG 2 cut(s) 1399, 2161
MflI RGATCY 4 cut(s) 340, 492, 2024, 2459
MhlI GDGCHC 6 cut(s) 91, 302, 1623, 1925, 2489, 2742
MlsI TGGCCA 2 cut(s) 442, 1449
MluNI TGGCCA 2 cut(s) 442, 1449
MlyI GAGTC 4 cut(s) 61, 593, 974, 3009
MmeI TCCRAC 5 cut(s) 416, 449, 590, 1357, 1836
Mox20I TGGCCA 2 cut(s) 442, 1449
Mph1103I ATGCAT 1 cut(s) 2398
MroNI GCCGGC 2 cut(s) 357, 2494
MscI TGGCCA 2 cut(s) 442, 1449
MslI CAYNNNNRTG 3 cut(s) 769, 1155, 2255
Msp20I TGGCCA 2 cut(s) 442, 1449
MspA1I CMGCKG 3 cut(s) 1716, 2387, 2663
MspI CCGG 8 cut(s) 15, 50, 57, 71, 358, 1232, 1585, 2495
MspR9I CCNGG 5 cut(s) 177, 490, 1020, 1185, 1986
MunI CAATTG 2 cut(s) 1399, 2161
Mva1269I GAATGC 4 cut(s) 1679, 2119, 2398, 2878
MvaI CCWGG 5 cut(s) 177, 490, 1020, 1185, 1986
NaeI GCCGGC 2 cut(s) 359, 2496
NcoI CCATGG 2 cut(s) 1070, 2400
NgoMIV GCCGGC 2 cut(s) 357, 2494
NlaIV GGNNCC 8 cut(s) 342, 494, 689, 819, 2550, 2605, 2740, 2741
NmeAIII GCCGAG 2 cut(s) 424, 2549
NmuCI GTSAC 6 cut(s) 17, 59, 281, 1157, 1273, 1497
NsbI TGCGCA 2 cut(s) 1993, 3106
NsiI ATGCAT 1 cut(s) 2398
NspI RCATGY 5 cut(s) 357, 526, 1115, 1757, 3105
NspV TTCGAA 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 1155
PaeI GCATGC 2 cut(s) 357, 3105
PciI ACATGT 1 cut(s) 522
PctI GAATGC 4 cut(s) 1679, 2119, 2398, 2878
PdiI GCCGGC 2 cut(s) 359, 2496
PfoI TCCNGGA 1 cut(s) 488
PinAI ACCGGT 1 cut(s) 14
PkrI GCNGC 9 cut(s) 55, 76, 232, 661, 1711, 1718, 2874, 2877, 2913
PleI GAGTC 4 cut(s) 61, 592, 973, 3009
PpsI GAGTC 4 cut(s) 61, 592, 973, 3009
Ppu21I YACGTR 1 cut(s) 1870
PpuMI RGGWCCY 1 cut(s) 1543
PscI ACATGT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 1543
Psp6I CCWGG 5 cut(s) 175, 488, 1018, 1183, 1984
PspEI GGTNACC 2 cut(s) 17, 1157
PspFI CCCAGC 3 cut(s) 2471, 2591, 2673
PspGI CCWGG 5 cut(s) 175, 488, 1018, 1183, 1984
PspN4I GGNNCC 8 cut(s) 342, 494, 689, 819, 2550, 2605, 2740, 2741
PspOMI GGGCCC 1 cut(s) 2738
PspPI GGNCC 7 cut(s) 1120, 1543, 2548, 2603, 2738, 2739, 2821
PspPPI RGGWCCY 1 cut(s) 1543
PsrI GAACNNNNNNTAC 2 cut(s) 766, 798
PstI CTGCAG 3 cut(s) 649, 1459, 2059
PsuI RGATCY 4 cut(s) 340, 492, 2024, 2459
PvuII CAGCTG 2 cut(s) 1716, 2387
RsaI GTAC 7 cut(s) 1029, 1314, 2230, 2685, 2852, 2869, 2889
RsaNI GTAC 7 cut(s) 1028, 1313, 2229, 2684, 2851, 2868, 2888
RseI CAYNNNNRTG 3 cut(s) 769, 1155, 2255
SacI GAGCTC 1 cut(s) 91
SatI GCNGC 9 cut(s) 54, 75, 231, 660, 1710, 1717, 2873, 2876, 2912
Sau96I GGNCC 7 cut(s) 1120, 1543, 2548, 2603, 2738, 2739, 2821
ScaI AGTACT 1 cut(s) 2852
SchI GAGTC 4 cut(s) 61, 593, 974, 3009
ScrFI CCNGG 5 cut(s) 177, 490, 1020, 1185, 1986
SduI GDGCHC 6 cut(s) 91, 302, 1623, 1925, 2489, 2742
SfaNI GCATC 9 cut(s) 364, 950, 1330, 1849, 1886, 2405, 2578, 2985, 3114
SfcI CTRYAG 6 cut(s) 645, 1455, 1807, 1902, 2055, 2536
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 4 cut(s) 1120, 1543, 2548, 2603
SmiMI CAYNNNNRTG 3 cut(s) 769, 1155, 2255
SmlI CTYRAG 1 cut(s) 634
SmoI CTYRAG 1 cut(s) 634
SphI GCATGC 2 cut(s) 357, 3105
SsiI CCGC 3 cut(s) 1804, 2663, 2912
SspMI CTAG 9 cut(s) 333, 603, 957, 1064, 2040, 2226, 2277, 2726, 2903
SstI GAGCTC 1 cut(s) 91
StyD4I CCNGG 5 cut(s) 175, 488, 1018, 1183, 1984
StyI CCWWGG 3 cut(s) 936, 1070, 2400
TaaI ACNGT 8 cut(s) 1472, 1497, 1811, 2233, 2540, 2562, 2751, 3146
TaiI ACGT 5 cut(s) 487, 1872, 2202, 2285, 2329
TaqI TCGA 8 cut(s) 319, 408, 1324, 1461, 2419, 2432, 2586, 2988
TaqII GACCGA 2 cut(s) 686, 1749
TatI WGTACW 3 cut(s) 1027, 2228, 2850
TauI GCSGC 1 cut(s) 2914
TscAI CASTG 5 cut(s) 1484, 1500, 1669, 1930, 2545
TseFI GTSAC 6 cut(s) 17, 59, 281, 1157, 1273, 1497
TseI GCWGC 8 cut(s) 53, 74, 230, 659, 1709, 1716, 2872, 2875
Tsp45I GTSAC 6 cut(s) 17, 59, 281, 1157, 1273, 1497
TspGWI ACGGA 1 cut(s) 2731
TspRI CASTG 5 cut(s) 1484, 1500, 1669, 1930, 2545
VneI GTGCAC 2 cut(s) 1921, 2485
VpaK11BI GGWCC 4 cut(s) 1120, 1543, 2548, 2603
XagI CCTNNNNNAGG 2 cut(s) 1363, 2612
XbaI TCTAGA 1 cut(s) 2725
XceI RCATGY 5 cut(s) 357, 526, 1115, 1757, 3105
XmiI GTMKAC 2 cut(s) 138, 2713
XspI CTAG 9 cut(s) 333, 603, 957, 1064, 2040, 2226, 2277, 2726, 2903
ZrmI AGTACT 1 cut(s) 2852
Zsp2I ATGCAT 1 cut(s) 2398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.