Rmu_sc0004928.1_g000018

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004928.1
OG0008176
Physical Location & Seq
Reverse (-)
61839 .. 63126
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
atggcttctattgtgactacttctctccaaacaaggcttataaaccaaagatgtggactgaagcctagattggatggtaagttattgaggcttgtgacctatacttggtcggtgtatcaagatggggagccgatagaggaagatagaactgccgtactagaactcatggatatccgcaaacacttcacagcaaaatatggaaaggagtttgtctctatagctgttgaactacggccggatagtggtgtaagccgcatgttaatagagaaattatctaccaaatcacttgatcgccctaccaagatgaaaattttggctgcaattgcagaggaacataatgtcaaatgggatcccgaggcatttgaagagaagcctcctgaggacctgctgaacggaccaaatacatttggaatggccagtatggattctgctccacctccacctactcatgatgtcaggggacctcctaatgtccaagttccgcctaatagcatggccagtaaagctcatgtccacctgatcaacctccaattgctcaagttccacgcagaaattatgagtatcacgatggacctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

192

Amino Acids

21.55

Weight (kDa)

6.31

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 51 - 115 1.1e-13 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 393
AciI CCGC 3 cut(s) 175, 253, 482
AclWI GGATC 2 cut(s) 344, 357
AcoI YGGCCR 3 cut(s) 233, 414, 495
AcsI RAATTY 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 80
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 105, 242
AgsI TTSAA 2 cut(s) 227, 365
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 2 cut(s) 221, 506
AluI AGCT 2 cut(s) 221, 506
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 2 cut(s) 344, 357
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 233, 414, 495
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 309
AspS9I GGNCC 4 cut(s) 382, 395, 461, 571
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 4 cut(s) 382, 395, 461, 571
AxyI CCTNAGG 1 cut(s) 378
BalI TGGCCA 2 cut(s) 416, 497
BamHI GGATCC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 304
BccI CCATC 3 cut(s) 68, 116, 562
BceAI ACGGC 2 cut(s) 137, 248
BclI TGATCA 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 2 cut(s) 66, 158
BfmI CTRYAG 1 cut(s) 216
BfuAI ACCTGC 1 cut(s) 393
BisI GCNGC 2 cut(s) 253, 318
BlsI GCNGC 2 cut(s) 254, 319
Bme18I GGWCC 4 cut(s) 382, 395, 461, 571
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 4 cut(s) 382, 395, 461, 571
BmiI GGNNCC 3 cut(s) 129, 351, 462
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpuEI CTTGAG 1 cut(s) 521
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 242
Bse1I ACTGG 2 cut(s) 417, 498
Bse21I CCTNAGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 79
BseLI CCNNNNNNNGG 2 cut(s) 105, 242
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 417, 498
BseX3I CGGCCG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 3 cut(s) 235, 416, 497
BsiEI CGRYCG 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 2 cut(s) 105, 242
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 3 cut(s) 235, 416, 497
BsoBI CYCGRG 1 cut(s) 353
Bsp143I GATC 3 cut(s) 289, 349, 519
BspACI CCGC 3 cut(s) 175, 253, 482
BspANI GGCC 3 cut(s) 235, 416, 497
BspCNI CTCAG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 129, 351, 462
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 344, 357
BsrI ACTGG 2 cut(s) 417, 498
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 3 cut(s) 289, 349, 519
Bst6I CTCTTC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 79
BstKTI GATC 3 cut(s) 292, 352, 522
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 3 cut(s) 289, 349, 519
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 2 cut(s) 323, 503
BstNSI RCATGY 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 349
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 233
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 3 cut(s) 235, 416, 497
BtsCI GGATG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 393
CciI TCATGA 1 cut(s) 448
Cfr13I GGNCC 4 cut(s) 382, 395, 461, 571
Csp6I GTAC 1 cut(s) 155
CviAII CATG 5 cut(s) 166, 256, 449, 493, 509
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 291, 351, 521
DpnII GATC 3 cut(s) 289, 349, 519
EaeI YGGCCR 3 cut(s) 233, 414, 495
EagI CGGCCG 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 471
EclXI CGGCCG 1 cut(s) 233
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 4 cut(s) 382, 395, 461, 571
Eco52I CGGCCG 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 80
Eco81I CCTNAGG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 382, 461
EcoRV GATATC 1 cut(s) 172
FaeI CATG 5 cut(s) 169, 259, 452, 496, 512
FaqI GGGAC 1 cut(s) 474
FatI CATG 5 cut(s) 165, 255, 448, 492, 508
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 2 cut(s) 253, 318
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 253, 318
FspBI CTAG 2 cut(s) 66, 158
GluI GCNGC 2 cut(s) 253, 318
HaeIII GGCC 3 cut(s) 235, 416, 497
HapII CCGG 1 cut(s) 236
Hin1II CATG 5 cut(s) 169, 259, 452, 496, 512
HinfI GANTC 1 cut(s) 425
HpaII CCGG 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 56, 514
Hpy188III TCNNGA 5 cut(s) 119, 353, 377, 449, 565
Hpy8I GTNNAC 2 cut(s) 56, 514
HpyCH4V TGCA 2 cut(s) 320, 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 503
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 5 cut(s) 169, 259, 452, 496, 512
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 3 cut(s) 289, 349, 519
LmnI GCTCC 2 cut(s) 127, 436
LpnPI CCDG 7 cut(s) 249, 390, 398, 430, 442, 511, 530
Lsp1109I GCAGC 1 cut(s) 304
MaeI CTAG 2 cut(s) 66, 158
MaeIII GTNAC 2 cut(s) 13, 94
MalI GATC 3 cut(s) 291, 351, 521
MboI GATC 3 cut(s) 289, 349, 519
MboII GAAGA 2 cut(s) 152, 377
MfeI CAATTG 2 cut(s) 321, 530
MflI RGATCY 1 cut(s) 349
MlsI TGGCCA 2 cut(s) 416, 497
MluCI AATT 5 cut(s) 269, 309, 321, 530, 552
MluNI TGGCCA 2 cut(s) 416, 497
MnlI CCTC 9 cut(s) 81, 130, 322, 349, 373, 384, 447, 474, 536
Mox20I TGGCCA 2 cut(s) 416, 497
MscI TGGCCA 2 cut(s) 416, 497
MseI TTAA 1 cut(s) 260
Msp20I TGGCCA 2 cut(s) 416, 497
MspI CCGG 1 cut(s) 236
MunI CAATTG 2 cut(s) 321, 530
MwoI GCNNNNNNNGC 2 cut(s) 323, 503
NdeII GATC 3 cut(s) 289, 349, 519
NlaIII CATG 5 cut(s) 169, 259, 452, 496, 512
NlaIV GGNNCC 3 cut(s) 129, 351, 462
NmuCI GTSAC 2 cut(s) 13, 94
NspI RCATGY 1 cut(s) 259
PagI TCATGA 1 cut(s) 448
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 2 cut(s) 254, 319
PpuMI RGGWCCY 2 cut(s) 382, 461
PsiI TTATAA 1 cut(s) 41
Psp5II RGGWCCY 2 cut(s) 382, 461
PspN4I GGNNCC 3 cut(s) 129, 351, 462
PspPI GGNCC 4 cut(s) 382, 395, 461, 571
PspPPI RGGWCCY 2 cut(s) 382, 461
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 2 cut(s) 253, 318
Sau3AI GATC 3 cut(s) 289, 349, 519
Sau96I GGNCC 4 cut(s) 382, 395, 461, 571
SfcI CTRYAG 1 cut(s) 216
SinI GGWCC 4 cut(s) 382, 395, 461, 571
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 5 cut(s) 269, 309, 321, 530, 552
SsiI CCGC 3 cut(s) 175, 253, 482
SspMI CTAG 2 cut(s) 66, 158
TasI AATT 5 cut(s) 269, 309, 321, 530, 552
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TseFI GTSAC 2 cut(s) 13, 94
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 2 cut(s) 13, 94
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 1 cut(s) 408
VpaK11BI GGWCC 4 cut(s) 382, 395, 461, 571
XapI RAATTY 1 cut(s) 309
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 66, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.