Rmu_sc0005901.1_g000001

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005901.1
OG0004570
Physical Location & Seq
Reverse (-)
1 .. 2003
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
atggcacctgcgccgctgccggcaaccaccccacccatgaaggccacttcaaatggatcatttcaaggtgaaaatccacttgattacgctcttcccttactgatcctccaaatcatcttggtcgtggcgttcaccaggtttcttgcatatcttctaaaacctctccggcaaccccgagtcatagccgaagttataggcggatgtctgctgggcccctccgcattcgggaggagcaagacctttctacacatgattttcccgccaaaaggcatgacggtgctagacacggtagccaacatcgggctctgcttcttcctcttcctcgtcggccttgagctcgacatcggcgccatccgtcgcacaggaaagaagtccctgggcatcgccttctgcgggatcaccctccccttcgtcctcggcatcggcacctccgaagccctacgcgccactgtctcgaaaggcgtgaaacaaggccccttcctcgttttcaacggcgtcgccctctccatcaccgccttcccagtcctcgcccgcatcctcgctgagctcaagctcctcaccaccgacgtcggccgcatcgccatgtccgccgccgccgtcaacgacgtcgccgcctggatccttctagccctcgccatcgccctcaccggcaacaacacctccccgctcgtctccgtctgggtcctcctctgcggtgccggcttcgtcgggttcgccgtcgtcgccatccgccccatactggtcatgatggcgcgaaggtcccaggagggcgaacccgtgaaggagctctacatctgcatcactctctcgttggttttggccgcggggttcgtgacggacacgattgggatacacgcgctgttcggcgcgttcgtggtggggatcataatgccgaaggagggtccgttcgcgggtgtgttgatagagaagattgaggacctggtttcggggcttctgttgccgctttattttgtatccagtgggctcaagactaattttttgactataagcgggggggtgtcgtggggtatgctggtgctggtgatatgtacagcgtcgtttgggaagatcgtcgggactgtcactgtgtcgatgctgttcaaggtgcctttcagggaagccttggcgcttggcttccttatgaatacaaagggcttggtggagctcatcgtcctcaatattggcaaggatagaaag

Protein Analysis

399

Amino Acids

42.22

Weight (kDa)

9.51

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 45 - 397 3.8e-55 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
AatII GACGTC 2 cut(s) 570, 609
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 5 cut(s) 4, 347, 425, 695, 1105
AccBSI CCGCTC 1 cut(s) 667
AccII CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
AclWI GGATC 6 cut(s) 64, 97, 404, 613, 626, 890
AcoI YGGCCR 2 cut(s) 571, 819
AcyI GRCGYC 4 cut(s) 348, 495, 567, 606
AfaI GTAC 1 cut(s) 1051
AfiI CCNNNNNNNGG 8 cut(s) 225, 266, 300, 393, 739, 899, 911, 946
AgsI TTSAA 4 cut(s) 51, 65, 490, 1102
AjnI CCWGG 5 cut(s) 134, 375, 614, 762, 939
AloI GAACNNNNNNTCC 2 cut(s) 890, 922
AluBI AGCT 5 cut(s) 337, 547, 553, 787, 1165
AluI AGCT 5 cut(s) 337, 547, 553, 787, 1165
Alw21I GWGCWC 4 cut(s) 339, 549, 789, 1167
Alw26I GTCTC 2 cut(s) 457, 676
AlwI GGATC 6 cut(s) 64, 97, 404, 613, 626, 890
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 6 cut(s) 42, 211, 328, 472, 571, 819
ApaI GGGCCC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 982, 1014
AspLEI GCGC 7 cut(s) 13, 350, 446, 754, 859, 869, 1129
AspS9I GGNCC 7 cut(s) 211, 212, 473, 682, 759, 902, 937
AsuHPI GGTGA 7 cut(s) 80, 124, 391, 502, 550, 637, 1054
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 4 cut(s) 682, 759, 902, 937
BaeGI GKGCMC 1 cut(s) 215
BamHI GGATCC 1 cut(s) 618
BanI GGYRCC 5 cut(s) 4, 347, 425, 695, 1105
BanII GRGCYC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
BarI GAAGNNNNNNTAC 2 cut(s) 773, 805
Bbv12I GWGCWC 4 cut(s) 339, 549, 789, 1167
BbvI GCAGC 1 cut(s) 3
BccI CCATC 5 cut(s) 359, 515, 644, 734, 742
BceAI ACGGC 3 cut(s) 508, 581, 701
BciT130I CCWGG 5 cut(s) 136, 377, 616, 764, 941
BciVI GTATCC 2 cut(s) 843, 985
BcoDI GTCTC 2 cut(s) 457, 676
BfaI CTAG 2 cut(s) 281, 626
BfoI RGCGCY 2 cut(s) 351, 1130
BfuAI ACCTGC 1 cut(s) 16
BfuI GTATCC 2 cut(s) 843, 985
BisI GCNGC 8 cut(s) 14, 17, 574, 591, 594, 612, 822, 962
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 8 cut(s) 15, 18, 575, 592, 595, 613, 823, 963
Bme1390I CCNGG 5 cut(s) 136, 377, 616, 764, 941
Bme18I GGWCC 4 cut(s) 682, 759, 902, 937
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 7 cut(s) 211, 212, 473, 682, 759, 902, 937
BmrFI CCNGG 5 cut(s) 136, 377, 616, 764, 941
BmrI ACTGGG 1 cut(s) 515
BmsI GCATC 6 cut(s) 390, 429, 543, 585, 807, 1083
BmuI ACTGGG 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 543
BpuEI CTTGAG 3 cut(s) 353, 533, 971
BsaHI GRCGYC 4 cut(s) 348, 495, 567, 606
BsaJI CCNNGG 6 cut(s) 375, 376, 415, 762, 822, 1122
BsaXI ACNNNNNCTCC 6 cut(s) 90, 120, 644, 674, 890, 920
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 266, 300, 393, 739, 899, 911, 946
Bse118I RCCGGY 3 cut(s) 19, 647, 698
Bse1I ACTGG 3 cut(s) 521, 744, 978
BseBI CCWGG 5 cut(s) 136, 377, 616, 764, 941
BseDI CCNNGG 6 cut(s) 375, 376, 415, 762, 822, 1122
BseGI GGATG 4 cut(s) 206, 351, 534, 726
BseLI CCNNNNNNNGG 8 cut(s) 225, 266, 300, 393, 739, 899, 911, 946
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 3 cut(s) 521, 744, 978
BseRI GAGGAG 3 cut(s) 244, 545, 677
BseSI GKGCMC 1 cut(s) 215
BseX3I CGGCCG 1 cut(s) 571
BseXI GCAGC 1 cut(s) 3
BseYI CCCAGC 1 cut(s) 208
Bsh1236I CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 6 cut(s) 44, 213, 330, 474, 573, 821
BshNI GGYRCC 5 cut(s) 4, 347, 425, 695, 1105
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 4 cut(s) 339, 549, 789, 1167
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 4 cut(s) 20, 166, 648, 699
BslFI GGGAC 3 cut(s) 358, 745, 1090
BslI CCNNNNNNNGG 8 cut(s) 225, 266, 300, 393, 739, 899, 911, 946
BsmAI GTCTC 2 cut(s) 457, 676
BsmBI CGTCTC 1 cut(s) 676
BsmFI GGGAC 3 cut(s) 358, 745, 1090
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 6 cut(s) 44, 213, 330, 474, 573, 821
BsoBI CYCGRG 1 cut(s) 174
Bsp120I GGGCCC 1 cut(s) 211
Bsp1286I GDGCHC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
Bsp1407I TGTACA 1 cut(s) 1049
Bsp143I GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
Bsp1720I GCTNAGC 1 cut(s) 543
BspANI GGCC 6 cut(s) 44, 213, 330, 474, 573, 821
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
BspHI TCATGA 1 cut(s) 744
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 6 cut(s) 64, 97, 404, 613, 626, 890
BspQI GCTCTTC 1 cut(s) 96
BspT107I GGYRCC 5 cut(s) 4, 347, 425, 695, 1105
BsrBI CCGCTC 1 cut(s) 667
BsrFI RCCGGY 3 cut(s) 19, 647, 698
BsrGI TGTACA 1 cut(s) 1049
BsrI ACTGG 3 cut(s) 521, 744, 978
BssAI RCCGGY 3 cut(s) 19, 647, 698
BssECI CCNNGG 6 cut(s) 375, 376, 415, 762, 822, 1122
BssMI GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
BssNI GRCGYC 4 cut(s) 348, 495, 567, 606
BssT1I CCWWGG 1 cut(s) 1122
Bst2UI CCWGG 5 cut(s) 136, 377, 616, 764, 941
Bst4CI ACNGT 5 cut(s) 277, 289, 451, 1081, 1087
Bst6I CTCTTC 2 cut(s) 96, 323
BstACI GRCGYC 4 cut(s) 348, 495, 567, 606
BstAUI TGTACA 1 cut(s) 1049
BstC8I GCNNGC 3 cut(s) 21, 532, 700
BstDEI CTNAG 1 cut(s) 543
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 4 cut(s) 206, 351, 534, 726
BstFNI CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
BstH2I RGCGCY 2 cut(s) 351, 1130
BstHHI GCGC 7 cut(s) 13, 350, 446, 754, 859, 869, 1129
BstKTI GATC 6 cut(s) 59, 105, 399, 621, 885, 1071
BstMAI GTCTC 2 cut(s) 457, 676
BstMBI GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 5 cut(s) 443, 587, 699, 722, 958
BstNI CCWGG 5 cut(s) 136, 377, 616, 764, 941
BstSCI CCNGG 5 cut(s) 134, 375, 614, 762, 939
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
BstV1I GCAGC 1 cut(s) 3
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
BstZI CGGCCG 1 cut(s) 571
BsuI GTATCC 2 cut(s) 843, 985
BsuRI GGCC 6 cut(s) 44, 213, 330, 474, 573, 821
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 3 cut(s) 367, 562, 622
BtsCI GGATG 4 cut(s) 206, 351, 534, 726
BtsIMutI CAGTG 3 cut(s) 447, 985, 1083
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 3 cut(s) 21, 532, 700
CciI TCATGA 1 cut(s) 744
CfoI GCGC 7 cut(s) 13, 350, 446, 754, 859, 869, 1129
Cfr10I RCCGGY 3 cut(s) 19, 647, 698
Cfr13I GGNCC 7 cut(s) 211, 212, 473, 682, 759, 902, 937
Cfr42I CCGCGG 1 cut(s) 825
CseI GACGC 2 cut(s) 484, 1044
CsiI ACCWGGT 2 cut(s) 134, 939
Csp6I GTAC 1 cut(s) 1050
CviAII CATG 5 cut(s) 37, 250, 271, 583, 745
CviQI GTAC 1 cut(s) 1050
DdeI CTNAG 1 cut(s) 543
DinI GGCGCC 1 cut(s) 349
DpnI GATC 6 cut(s) 58, 104, 398, 620, 884, 1070
DpnII GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
EaeI YGGCCR 2 cut(s) 571, 819
EagI CGGCCG 1 cut(s) 571
Eam1104I CTCTTC 2 cut(s) 96, 323
EarI CTCTTC 2 cut(s) 96, 323
EciI GGCGGA 3 cut(s) 213, 577, 719
Ecl136II GAGCTC 4 cut(s) 337, 547, 787, 1165
EclXI CGGCCG 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 1122
Eco24I GRGCYC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
Eco47I GGWCC 4 cut(s) 682, 759, 902, 937
Eco52I CGGCCG 1 cut(s) 571
Eco53kI GAGCTC 4 cut(s) 337, 547, 787, 1165
Eco88I CYCGRG 1 cut(s) 174
EcoICRI GAGCTC 4 cut(s) 337, 547, 787, 1165
EcoO109I RGGNCCY 5 cut(s) 212, 473, 682, 759, 937
EcoRII CCWGG 5 cut(s) 134, 375, 614, 762, 939
EcoT14I CCWWGG 1 cut(s) 1122
EcoT38I GRGCYC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 1122
Esp3I CGTCTC 1 cut(s) 676
FaeI CATG 5 cut(s) 40, 253, 274, 586, 748
FaqI GGGAC 3 cut(s) 358, 745, 1090
FatI CATG 5 cut(s) 36, 249, 270, 582, 744
FauI CCCGC 7 cut(s) 267, 386, 539, 672, 817, 904, 1004
Fnu4HI GCNGC 8 cut(s) 14, 17, 574, 591, 594, 612, 822, 962
FokI GGATG 4 cut(s) 213, 338, 521, 713
FriOI GRGCYC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
Fsp4HI GCNGC 8 cut(s) 14, 17, 574, 591, 594, 612, 822, 962
FspBI CTAG 2 cut(s) 281, 626
GlaI GCGC 7 cut(s) 12, 349, 445, 753, 858, 868, 1128
GluI GCNGC 8 cut(s) 14, 17, 574, 591, 594, 612, 822, 962
GsaI CCCAGC 1 cut(s) 212
HaeII RGCGCY 2 cut(s) 351, 1130
HaeIII GGCC 6 cut(s) 44, 213, 330, 474, 573, 821
HapII CCGG 4 cut(s) 20, 166, 648, 699
HgaI GACGC 2 cut(s) 484, 1044
HhaI GCGC 7 cut(s) 13, 350, 446, 754, 859, 869, 1129
Hin1I GRCGYC 4 cut(s) 348, 495, 567, 606
Hin1II CATG 5 cut(s) 40, 253, 274, 586, 748
Hin6I GCGC 7 cut(s) 11, 348, 444, 752, 857, 867, 1127
HinP1I GCGC 7 cut(s) 11, 348, 444, 752, 857, 867, 1127
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 1 cut(s) 177
HpaII CCGG 4 cut(s) 20, 166, 648, 699
HphI GGTGA 7 cut(s) 80, 124, 391, 502, 550, 637, 1054
Hpy166II GTNNAC 2 cut(s) 132, 601
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 6 cut(s) 226, 454, 745, 832, 988, 1075
Hpy8I GTNNAC 2 cut(s) 132, 601
HpyAV CCTTC 9 cut(s) 34, 397, 418, 487, 526, 632, 750, 775, 889
HpyCH4III ACNGT 5 cut(s) 277, 289, 451, 1081, 1087
HpyCH4IV ACGT 2 cut(s) 567, 606
HpyCH4V TGCA 2 cut(s) 146, 798
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 587, 699, 722, 958
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 2 cut(s) 567, 606
Hsp92I GRCGYC 4 cut(s) 348, 495, 567, 606
Hsp92II CATG 5 cut(s) 40, 253, 274, 586, 748
HspAI GCGC 7 cut(s) 11, 348, 444, 752, 857, 867, 1127
KasI GGCGCC 1 cut(s) 347
KroI GCCGGC 2 cut(s) 19, 698
KroNI GCCGGC 2 cut(s) 21, 700
KspI CCGCGG 1 cut(s) 825
Kzo9I GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 4 cut(s) 231, 558, 784, 1162
Lsp1109I GCAGC 1 cut(s) 3
LweI GCATC 6 cut(s) 390, 429, 543, 585, 807, 1083
MabI ACCWGGT 2 cut(s) 134, 939
MaeI CTAG 2 cut(s) 281, 626
MaeII ACGT 2 cut(s) 567, 606
MaeIII GTNAC 2 cut(s) 832, 1081
MalI GATC 6 cut(s) 58, 104, 398, 620, 884, 1070
MbiI CCGCTC 1 cut(s) 667
MboI GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
MboII GAAGA 6 cut(s) 83, 143, 304, 310, 940, 1078
MflI RGATCY 1 cut(s) 618
MhlI GDGCHC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
MluCI AATT 1 cut(s) 994
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 1 cut(s) 186
MroNI GCCGGC 2 cut(s) 19, 698
MslI CAYNNNNRTG 2 cut(s) 275, 581
MspA1I CMGCKG 2 cut(s) 16, 824
MspI CCGG 4 cut(s) 20, 166, 648, 699
MspR9I CCNGG 5 cut(s) 136, 377, 616, 764, 941
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 5 cut(s) 136, 377, 616, 764, 941
MvnI CGCG 6 cut(s) 444, 754, 824, 857, 869, 911
MwoI GCNNNNNNNGC 5 cut(s) 443, 587, 699, 722, 958
NaeI GCCGGC 2 cut(s) 21, 700
NarI GGCGCC 1 cut(s) 348
NdeII GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
NgoMIV GCCGGC 2 cut(s) 19, 698
NlaIII CATG 5 cut(s) 40, 253, 274, 586, 748
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 832, 1081
PagI TCATGA 1 cut(s) 744
PaqCI CACCTGC 1 cut(s) 16
PasI CCCWGGG 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 3 cut(s) 429, 714, 870
PctI GAATGC 1 cut(s) 221
PdiI GCCGGC 2 cut(s) 21, 700
PkrI GCNGC 8 cut(s) 15, 18, 575, 592, 595, 613, 823, 963
PleI GAGTC 1 cut(s) 185
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 185
PpuMI RGGWCCY 3 cut(s) 682, 759, 937
Psp124BI GAGCTC 4 cut(s) 339, 549, 789, 1167
Psp5II RGGWCCY 3 cut(s) 682, 759, 937
Psp6I CCWGG 5 cut(s) 134, 375, 614, 762, 939
PspFI CCCAGC 1 cut(s) 208
PspGI CCWGG 5 cut(s) 134, 375, 614, 762, 939
PspOMI GGGCCC 1 cut(s) 211
PspPI GGNCC 7 cut(s) 211, 212, 473, 682, 759, 902, 937
PspPPI RGGWCCY 3 cut(s) 682, 759, 937
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 1 cut(s) 1051
RsaNI GTAC 1 cut(s) 1050
RseI CAYNNNNRTG 2 cut(s) 275, 581
SacI GAGCTC 4 cut(s) 339, 549, 789, 1167
SacII CCGCGG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 8 cut(s) 14, 17, 574, 591, 594, 612, 822, 962
Sau3AI GATC 6 cut(s) 56, 102, 396, 618, 882, 1068
Sau96I GGNCC 7 cut(s) 211, 212, 473, 682, 759, 902, 937
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 5 cut(s) 136, 377, 616, 764, 941
SduI GDGCHC 7 cut(s) 215, 306, 339, 549, 789, 987, 1167
SexAI ACCWGGT 2 cut(s) 134, 939
SfaNI GCATC 6 cut(s) 390, 429, 543, 585, 807, 1083
SfoI GGCGCC 1 cut(s) 349
Sfr303I CCGCGG 1 cut(s) 825
SgrBI CCGCGG 1 cut(s) 825
SinI GGWCC 4 cut(s) 682, 759, 902, 937
SmiMI CAYNNNNRTG 2 cut(s) 275, 581
SmlI CTYRAG 3 cut(s) 332, 548, 986
SmoI CTYRAG 3 cut(s) 332, 548, 986
Sse9I AATT 1 cut(s) 994
SspDI GGCGCC 1 cut(s) 347
SspI AATATT 1 cut(s) 1180
SspMI CTAG 2 cut(s) 281, 626
SstI GAGCTC 4 cut(s) 339, 549, 789, 1167
StyD4I CCNGG 5 cut(s) 134, 375, 614, 762, 939
StyI CCWWGG 1 cut(s) 1122
TaaI ACNGT 5 cut(s) 277, 289, 451, 1081, 1087
TaiI ACGT 2 cut(s) 570, 609
TaqI TCGA 3 cut(s) 339, 455, 1091
TasI AATT 1 cut(s) 994
TatI WGTACW 1 cut(s) 1049
TauI GCSGC 7 cut(s) 16, 576, 593, 596, 614, 824, 964
TscAI CASTG 3 cut(s) 454, 985, 1090
TseFI GTSAC 2 cut(s) 832, 1081
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 832, 1081
TspDTI ATGAA 2 cut(s) 53, 1157
TspGWI ACGGA 4 cut(s) 344, 664, 851, 894
TspRI CASTG 3 cut(s) 454, 985, 1090
VpaK11BI GGWCC 4 cut(s) 682, 759, 902, 937
XspI CTAG 2 cut(s) 281, 626
ZraI GACGTC 2 cut(s) 568, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.