Rmu_sc0006005.1_g000002

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006005.1
OG0004544
Physical Location & Seq
Forward (+)
1191 .. 4357
3167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
atgagactcctccgattacttcatcgcagcagcgtgcgggaattttgtggtggtctgaattggggtttggcgggaagaggtgtaattgtaaaggacaaagctttccaaaatttgaactcctcgcaactctatctccatggcgcaacagttgcagaatcactgtctggacttgcggtttatgttagaggaaatgtcaccggagaagctcgtaaaatttccaagccgcagtttggcaaaattttaaaacaggcacgtttgctctttctcttgttctctctttgcttagttaccaatcacatatcatccatgtcaaacatatatgtccatgatggggcagttggttcatcactcaaagctggtgcgaaagttcgcgttatacgtgatagcccatctgctgtattgtcactttccagcattctctgggggacacccacccgtgcagtctctcatgattctcgtcctttaacagtttatgtggctacttctataagtccagacattggagacattattggccttgaattgaaaggagataatgcacttatagctgctgatgttgagcgttcttcacttgttttatgtggcaatgccttctcagattcaaatgcaactaaacaagcactagctgccttgtctggacctgttatatttgcccgaagaggccttcccctgtctgcaaggcttttagtttttggtgattctgtgcttcttttatttgcaccagaagataccattaaggtgtgtggagatctgttggtgccaggagatataggtgttattctttcttctgaaggtgttgccccattgtttcaaactggaagctctggtagctcaaatctattcaagttgccagttgctgtagtccttgcaacttttgacagcagttctggtgttataccttctgtatcaaagcttgcacctggccaggcagcttatcacttcctggctggttatcaaaatgggaagttcatgcctgcatacagcaaaggcccttcatctattgacctgttggaactagcaaaggagtttctgttgaagttgaaagatgaccggatctcatcctacctggttaatgccaatggaggagaaaagggtgcaactggcaaagattttgctcggttggtggaatcaactctgtcaaataacatcccaccgttccaagcaaaaggtgggaatcttcaagacacatacaagagcttcttatccagcagatttggagaagtgccagaagaattctcattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

414

Amino Acids

43.91

Weight (kDa)

9.23

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 282 - 319 4.1e-06 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 757
AccB7I CCANNNNNTGG 1 cut(s) 500
AccII CGCG 1 cut(s) 372
AciI CCGC 4 cut(s) 37, 71, 173, 224
AclWI GGATC 1 cut(s) 1061
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 5 cut(s) 41, 109, 213, 237, 1232
AcuI CTGAAG 1 cut(s) 810
AfiI CCNNNNNNNGG 3 cut(s) 230, 331, 500
AgsI TTSAA 9 cut(s) 115, 521, 526, 603, 812, 844, 1036, 1042, 1181
AjnI CCWGG 5 cut(s) 760, 919, 924, 942, 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
Alw26I GTCTC 2 cut(s) 448, 498
AlwI GGATC 1 cut(s) 1061
AlwNI CAGNNNCTG 1 cut(s) 857
AoxI GGCC 4 cut(s) 514, 661, 922, 988
ApeKI GCWGC 5 cut(s) 27, 30, 548, 626, 929
ApoI RAATTY 5 cut(s) 41, 109, 213, 237, 1232
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 638, 989
AsuHPI GGTGA 2 cut(s) 187, 707
AvaII GGWCC 1 cut(s) 638
BalI TGGCCA 1 cut(s) 924
BanI GGYRCC 1 cut(s) 757
BbvI GCAGC 5 cut(s) 39, 42, 535, 613, 941
BccI CCATC 2 cut(s) 323, 397
BciT130I CCWGG 5 cut(s) 762, 921, 926, 944, 1067
BcoDI GTCTC 2 cut(s) 448, 498
BfaI CTAG 2 cut(s) 623, 1016
BfmI CTRYAG 1 cut(s) 858
BglII AGATCT 1 cut(s) 748
BisI GCNGC 6 cut(s) 28, 31, 224, 549, 627, 930
BlsI GCNGC 6 cut(s) 29, 32, 225, 550, 628, 931
Bme1390I CCNGG 5 cut(s) 762, 921, 926, 944, 1067
Bme18I GGWCC 1 cut(s) 638
BmgT120I GGNCC 2 cut(s) 638, 989
BmiI GGNNCC 1 cut(s) 759
BmrFI CCNGG 5 cut(s) 762, 921, 926, 944, 1067
BsaAI YACGTR 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 2 cut(s) 197, 1050
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 1016, 1046
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 331, 500
Bse1I ACTGG 3 cut(s) 820, 851, 1105
Bse3DI GCAATG 1 cut(s) 592
BseBI CCWGG 5 cut(s) 762, 921, 926, 944, 1067
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 302, 1058, 1146
BseLI CCNNNNNNNGG 3 cut(s) 230, 331, 500
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 3 cut(s) 820, 851, 1105
BseRI GAGGAG 2 cut(s) 109, 1098
BseXI GCAGC 5 cut(s) 39, 42, 535, 613, 941
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 4 cut(s) 516, 663, 924, 990
BshNI GGYRCC 1 cut(s) 757
BsiSI CCGG 2 cut(s) 198, 1051
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 3 cut(s) 230, 331, 500
BsmAI GTCTC 2 cut(s) 448, 498
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 4 cut(s) 516, 663, 924, 990
Bsp143I GATC 2 cut(s) 748, 1053
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 4 cut(s) 37, 71, 173, 224
BspANI GGCC 4 cut(s) 516, 663, 924, 990
BspCNI CTCAG 1 cut(s) 608
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 759
BspPI GGATC 1 cut(s) 1061
BspT107I GGYRCC 1 cut(s) 757
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 3 cut(s) 820, 851, 1105
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 748, 1053
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 5 cut(s) 762, 921, 926, 944, 1067
Bst4CI ACNGT 4 cut(s) 148, 162, 469, 1155
Bst6I CTCTTC 2 cut(s) 70, 652
BstAPI GCANNNNNTGC 2 cut(s) 149, 626
BstBAI YACGTR 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 35, 915, 975
BstDEI CTNAG 2 cut(s) 283, 595
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 302, 1058, 1146
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 751, 1056
BstMAI GTCTC 2 cut(s) 448, 498
BstMBI GATC 2 cut(s) 748, 1053
BstMWI GCNNNNNNNGC 4 cut(s) 149, 545, 626, 828
BstNI CCWGG 5 cut(s) 762, 921, 926, 944, 1067
BstSCI CCNGG 5 cut(s) 760, 919, 924, 942, 1065
BstSFI CTRYAG 1 cut(s) 858
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 5 cut(s) 39, 42, 535, 613, 941
BstX2I RGATCY 2 cut(s) 748, 1053
BstYI RGATCY 2 cut(s) 748, 1053
BsuRI GGCC 4 cut(s) 516, 663, 924, 990
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 8
BtsCI GGATG 3 cut(s) 302, 1058, 1146
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 3 cut(s) 35, 915, 975
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 638, 989
CsiI ACCWGGT 1 cut(s) 1065
CviAII CATG 5 cut(s) 137, 307, 326, 449, 970
DdeI CTNAG 2 cut(s) 283, 595
DpnI GATC 2 cut(s) 750, 1055
DpnII GATC 2 cut(s) 748, 1053
DraI TTTAAA 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 2 cut(s) 70, 652
EarI CTCTTC 2 cut(s) 70, 652
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 810
EcoO109I RGGNCCY 1 cut(s) 989
EcoRI GAATTC 1 cut(s) 1232
EcoRII CCWGG 5 cut(s) 760, 919, 924, 942, 1065
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 5 cut(s) 140, 310, 329, 452, 973
FalI AAGNNNNNCTT 2 cut(s) 1184, 1216
FaqI GGGAC 1 cut(s) 439
FatI CATG 5 cut(s) 136, 306, 325, 448, 969
FauI CCCGC 2 cut(s) 30, 64
Fnu4HI GCNGC 6 cut(s) 28, 31, 224, 549, 627, 930
FokI GGATG 3 cut(s) 289, 1045, 1133
Fsp4HI GCNGC 6 cut(s) 28, 31, 224, 549, 627, 930
FspBI CTAG 2 cut(s) 623, 1016
GlaI GCGC 1 cut(s) 142
GluI GCNGC 6 cut(s) 28, 31, 224, 549, 627, 930
HaeIII GGCC 4 cut(s) 516, 663, 924, 990
HapII CCGG 2 cut(s) 198, 1051
HhaI GCGC 1 cut(s) 143
Hin1II CATG 5 cut(s) 140, 310, 329, 452, 973
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 99, 911
HinfI GANTC 7 cut(s) 6, 155, 452, 599, 698, 1127, 1174
HpaII CCGG 2 cut(s) 198, 1051
HphI GGTGA 2 cut(s) 187, 707
Hpy188I TCNGA 5 cut(s) 14, 57, 598, 790, 1244
Hpy188III TCNNGA 5 cut(s) 165, 449, 494, 636, 1181
HpyAV CCTTC 5 cut(s) 601, 674, 785, 909, 1002
HpyCH4III ACNGT 4 cut(s) 148, 162, 469, 1155
HpyCH4IV ACGT 2 cut(s) 253, 379
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 545, 626, 828
HpyF3I CTNAG 2 cut(s) 283, 595
HpySE526I ACGT 2 cut(s) 253, 379
Hsp92II CATG 5 cut(s) 140, 310, 329, 452, 973
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 2 cut(s) 748, 1053
Lsp1109I GCAGC 5 cut(s) 39, 42, 535, 613, 941
MabI ACCWGGT 1 cut(s) 1065
MaeI CTAG 2 cut(s) 623, 1016
MaeII ACGT 2 cut(s) 253, 379
MaeIII GTNAC 3 cut(s) 193, 286, 402
MalI GATC 2 cut(s) 750, 1055
MboI GATC 2 cut(s) 748, 1053
MboII GAAGA 7 cut(s) 87, 558, 669, 737, 777, 1169, 1241
MflI RGATCY 2 cut(s) 748, 1053
MlsI TGGCCA 1 cut(s) 924
MluCI AATT 8 cut(s) 41, 58, 84, 109, 213, 237, 521, 1232
MluNI TGGCCA 1 cut(s) 924
MmeI TCCRAC 1 cut(s) 990
MnlI CCTC 6 cut(s) 20, 71, 130, 179, 653, 1076
Mox20I TGGCCA 1 cut(s) 924
MscI TGGCCA 1 cut(s) 924
MseI TTAA 4 cut(s) 242, 464, 735, 1071
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 924
MspI CCGG 2 cut(s) 198, 1051
MspR9I CCNGG 5 cut(s) 762, 921, 926, 944, 1067
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 5 cut(s) 762, 921, 926, 944, 1067
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 149, 545, 626, 828
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 748, 1053
NlaIII CATG 5 cut(s) 140, 310, 329, 452, 973
NlaIV GGNNCC 1 cut(s) 759
NmuCI GTSAC 2 cut(s) 193, 402
PagI TCATGA 1 cut(s) 448
PceI AGGCCT 1 cut(s) 663
PcsI WCGNNNNNNNCGW 1 cut(s) 376
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 6 cut(s) 155, 452, 599, 698, 1127, 1174
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 6 cut(s) 29, 32, 225, 550, 628, 931
Ppu21I YACGTR 1 cut(s) 380
Psp6I CCWGG 5 cut(s) 760, 919, 924, 942, 1065
PspGI CCWGG 5 cut(s) 760, 919, 924, 942, 1065
PspN4I GGNNCC 1 cut(s) 759
PspPI GGNCC 2 cut(s) 638, 989
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 748, 1053
RseI CAYNNNNRTG 1 cut(s) 737
SaqAI TTAA 4 cut(s) 242, 464, 735, 1071
SatI GCNGC 6 cut(s) 28, 31, 224, 549, 627, 930
Sau3AI GATC 2 cut(s) 748, 1053
Sau96I GGNCC 2 cut(s) 638, 989
ScrFI CCNGG 5 cut(s) 762, 921, 926, 944, 1067
SexAI ACCWGGT 1 cut(s) 1065
SfcI CTRYAG 1 cut(s) 858
SinI GGWCC 1 cut(s) 638
SmiMI CAYNNNNRTG 1 cut(s) 737
Sse9I AATT 8 cut(s) 41, 58, 84, 109, 213, 237, 521, 1232
SseBI AGGCCT 1 cut(s) 663
SsiI CCGC 4 cut(s) 37, 71, 173, 224
SspMI CTAG 2 cut(s) 623, 1016
StuI AGGCCT 1 cut(s) 663
StyD4I CCNGG 5 cut(s) 760, 919, 924, 942, 1065
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 4 cut(s) 148, 162, 469, 1155
TaiI ACGT 2 cut(s) 256, 382
TasI AATT 8 cut(s) 41, 58, 84, 109, 213, 237, 521, 1232
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 6 cut(s) 155, 452, 599, 698, 1127, 1174
Tru1I TTAA 4 cut(s) 242, 464, 735, 1071
Tru9I TTAA 4 cut(s) 242, 464, 735, 1071
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 2 cut(s) 193, 402
TseI GCWGC 5 cut(s) 27, 30, 548, 626, 929
Tsp45I GTSAC 2 cut(s) 193, 402
TspDTI ATGAA 4 cut(s) 11, 333, 958, 984
TspRI CASTG 1 cut(s) 165
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 638
XapI RAATTY 5 cut(s) 41, 109, 213, 237, 1232
XcmI CCANNNNNNNNNTGG 1 cut(s) 1166
XspI CTAG 2 cut(s) 623, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.