Rmu_sc0006739.1_g000005

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006739.1
OG0004408
Physical Location & Seq
Forward (+)
21680 .. 24989
3310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
atggaaaacaaaaccctcatacataaaaggaaacggacaaaggtcgctaaggaaggtaggttggataaacgtccttgctccaacggaggtaatgagtttcccaagttggttgagaaagtagttgtcaaccgtgaggatgtcactgaggctgttgaagaaaatgtcaatctgaccaaggagccggagaatgaggagggggtagaatatgtggatttgacgatgtgtccacccacatcaaagtcaaaggtagctgaggttgaatgcaagttggcaataggttctgttgaacatattgttgcttgtgccactgttatggagtgcgaggatccttcccaacttgttcatggcactccactagggaatggcaatgtgcgcgtttctatctatgccgcgcttgaggagaaagcaaaagtgccatttcctgtgaaagatgaaattgaaacagtgaagcaggctatggggagttggggcattcctccccaacctagcaacaaggagtgtggatactttatcatgcgatacatgagagatatcattgaggataaagacatgtcattgtttcctacgaagctgcgcatggcatctgccgagctatctgtcgagccatcacacaacagttataacacatacccgttgcctgtttgtttatcagacaactgtgttccaccgttgtctgaattttcaacagacaacggctccctctacaacggtggcactccaaatcagataacagtttctgtctgtcgtctgataaggattttggcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

255

Amino Acids

28.26

Weight (kDa)

5.32

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 86 - 156 4.1e-08 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
Acc16I TGCGCA 1 cut(s) 575
AccII CGCG 2 cut(s) 375, 392
AciI CCGC 1 cut(s) 390
AclWI GGATC 2 cut(s) 320, 333
AcsI RAATTY 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 5 cut(s) 155, 260, 287, 440, 684
AhdI GACNNNNNGTC 1 cut(s) 222
AluBI AGCT 3 cut(s) 251, 571, 592
AluI AGCT 3 cut(s) 251, 571, 592
AlwI GGATC 2 cut(s) 320, 333
AlwNI CAGNNNCTG 1 cut(s) 737
ApeKI GCWGC 1 cut(s) 571
ApoI RAATTY 1 cut(s) 677
AspLEI GCGC 3 cut(s) 375, 394, 576
BamHI GGATCC 1 cut(s) 325
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 558
BccI CCATC 1 cut(s) 613
BceAI ACGGC 1 cut(s) 709
BciVI GTATCC 1 cut(s) 497
BfaI CTAG 2 cut(s) 356, 486
BfuI GTATCC 1 cut(s) 497
BisI GCNGC 2 cut(s) 390, 572
BlsI GCNGC 2 cut(s) 391, 573
BmeRI GACNNNNNGTC 1 cut(s) 222
BmiI GGNNCC 3 cut(s) 180, 327, 697
BmsI GCATC 1 cut(s) 590
BoxI GACNNNNGTC 1 cut(s) 41
Bpu10I CCTNAGC 2 cut(s) 48, 252
BpuEI CTTGAG 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 174
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bse3DI GCAATG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 2 cut(s) 135, 243
BseRI GAGGAG 2 cut(s) 206, 413
BseXI GCAGC 1 cut(s) 558
Bsh1236I CGCG 2 cut(s) 375, 392
BsiSI CCGG 1 cut(s) 182
BsmI GAATGC 2 cut(s) 266, 471
Bsp143I GATC 1 cut(s) 325
BspACI CCGC 1 cut(s) 390
BspCNI CTCAG 2 cut(s) 136, 244
BspFNI CGCG 2 cut(s) 375, 392
BspLI GGNNCC 3 cut(s) 180, 327, 697
BspPI GGATC 2 cut(s) 320, 333
BsrDI GCAATG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 8 cut(s) 131, 310, 445, 617, 659, 669, 710, 733
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 3 cut(s) 48, 144, 252
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 2 cut(s) 375, 392
BstHHI GCGC 3 cut(s) 375, 394, 576
BstKTI GATC 1 cut(s) 328
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 553
BstPAI GACNNNNGTC 1 cut(s) 41
BstUI CGCG 2 cut(s) 375, 392
BstV1I GCAGC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 1 cut(s) 325
BsuI GTATCC 1 cut(s) 497
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 3 cut(s) 141, 306, 450
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 3 cut(s) 375, 394, 576
CspCI CAANNNNNGTGG 2 cut(s) 481, 516
CviAII CATG 5 cut(s) 344, 514, 523, 550, 577
CviJI RGCY 8 cut(s) 149, 181, 251, 455, 571, 592, 604, 696
CviKI_1 RGCY 8 cut(s) 149, 181, 251, 455, 571, 592, 604, 696
DdeI CTNAG 3 cut(s) 48, 144, 252
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
DriI GACNNNNNGTC 1 cut(s) 222
Eam1105I GACNNNNNGTC 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 174
Eco32I GATATC 1 cut(s) 532
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 5 cut(s) 347, 517, 526, 553, 580
FatI CATG 5 cut(s) 343, 513, 522, 549, 576
Fnu4HI GCNGC 2 cut(s) 390, 572
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 390, 572
FspBI CTAG 2 cut(s) 356, 486
FspI TGCGCA 1 cut(s) 575
GlaI GCGC 3 cut(s) 374, 393, 575
GluI GCNGC 2 cut(s) 390, 572
HapII CCGG 1 cut(s) 182
HhaI GCGC 3 cut(s) 375, 394, 576
Hin1II CATG 5 cut(s) 347, 517, 526, 553, 580
Hin6I GCGC 3 cut(s) 373, 392, 574
HinP1I GCGC 3 cut(s) 373, 392, 574
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HpaII CCGG 1 cut(s) 182
Hpy166II GTNNAC 2 cut(s) 127, 227
Hpy188I TCNGA 5 cut(s) 171, 652, 676, 726, 750
Hpy8I GTNNAC 2 cut(s) 127, 227
HpyAV CCTTC 2 cut(s) 47, 339
HpyCH4III ACNGT 8 cut(s) 131, 310, 445, 617, 659, 669, 710, 733
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 3 cut(s) 48, 144, 252
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 347, 517, 526, 553, 580
HspAI GCGC 3 cut(s) 373, 392, 574
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 3 cut(s) 83, 178, 701
LpnPI CCDG 4 cut(s) 195, 435, 437, 651
Lsp1109I GCAGC 1 cut(s) 558
LweI GCATC 1 cut(s) 590
MaeI CTAG 2 cut(s) 356, 486
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 1 cut(s) 167
MflI RGATCY 1 cut(s) 325
MluCI AATT 2 cut(s) 435, 677
MmeI TCCRAC 2 cut(s) 42, 105
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 1 cut(s) 182
Mva1269I GAATGC 2 cut(s) 266, 471
MvnI CGCG 2 cut(s) 375, 392
MwoI GCNNNNNNNGC 1 cut(s) 372
NdeII GATC 1 cut(s) 325
NlaIII CATG 5 cut(s) 347, 517, 526, 553, 580
NlaIV GGNNCC 3 cut(s) 180, 327, 697
NmeAIII GCCGAG 1 cut(s) 613
NmuCI GTSAC 1 cut(s) 139
NsbI TGCGCA 1 cut(s) 575
NspI RCATGY 1 cut(s) 553
PciI ACATGT 1 cut(s) 549
PctI GAATGC 2 cut(s) 266, 471
PkrI GCNGC 2 cut(s) 391, 573
PscI ACATGT 1 cut(s) 549
PshAI GACNNNNGTC 1 cut(s) 41
PsiI TTATAA 1 cut(s) 621
PspN4I GGNNCC 3 cut(s) 180, 327, 697
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 311
SatI GCNGC 2 cut(s) 390, 572
Sau3AI GATC 1 cut(s) 325
SfaNI GCATC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 2 cut(s) 435, 677
SsiI CCGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 356, 486
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 8 cut(s) 131, 310, 445, 617, 659, 669, 710, 733
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 600
TasI AATT 2 cut(s) 435, 677
TauI GCSGC 1 cut(s) 392
TscAI CASTG 3 cut(s) 148, 313, 450
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 571
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 332, 447
TspGWI ACGGA 2 cut(s) 49, 99
TspRI CASTG 3 cut(s) 148, 313, 450
XapI RAATTY 1 cut(s) 677
XceI RCATGY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XspI CTAG 2 cut(s) 356, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.