Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0007474.1

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

81015 .. 88177

Gene Structure 7163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

atgagtctcactgctcttgacatttcccttgaaggatcacaatctccccatgatatcatgcatttcgtccccagaaggtatcaagtgaaagtgttcgaagtcgcaaagcgtaagaacataattgcagtgttggaaactggtgctgggaaaactatgattgctgtgatgctcatcaaacacattgcagatcaacacatcatcaagtccattggccacaagaagttgataattttcatggccccaacagttcatcttgtcattcagataatggtcatgacacctcaaattctcctggatgctttgcggaatgcatttttgaagatagagatgatttgcttgatagtctttgatgaatgccaccgtgccaccggcaatcatccctacacgaaaataatgaaggtttatacaatagaagacaggacagagattgaagagcatgttccctcagcagtccaaatttgtaaattttatgacccgacatggttgatctgtgtggatttgaaagccaaaatggaagctttatggtcaaaggtagttgatctgcatcatattgaatggaatttcttacatgtttttgctcaaacaacgagggataggcaagttctgtgccttattttcgtagagagaattgtcgcagccaaagtaatgcaaagatatgtgaagaaactgccatgtttatctcatttcacagcttcatatttgaccggaagtaccacatcagttgatgcacttgcaccgaaattgcagaaggaaattttggaatcattttacagtgggaaggtcaatctactatttgccactgatgtagttgaggagggaattcatgtccagaactgctcctgtgtgatccggtttgatcttcccaagacggttcgtagctatgttcagtctcggggacgagcacgaaaaaatgattcccaatatatcataatgcttgaaagggacaacaagaagcaaagagaccatctctttgacataatcaggagtgagtactcaatgacagatacatcgagaaatcgagatcctgatggatgcaatttaagagcttgtaactttgaagatgcaaatgcatactttgtagaagctactggagcatcagttactccagactctagtgtcagcctcatatatcggtactgtgaaaagctccctggagacaggtactttattcctaggccaaattttcacttcagactatttgaagggttgtatcagtgtgaaataacattgcctccaaatgcggcatttcaaacactagttgttcctatgagtagaaacggtcattcatcaaagcagcttgtaagcttggaagcttgtaaaatgctgcatcaaatgggtgctttggatgatcatcttctcccacttgtcgaagtgcctcttgataatgaagtgattgtgaaaagcaaagaattatctgcaggtgctggaactacaaaaaggaaagagttacatgggatgacttgcattcgtgcattaagtggaacttggagagagaaagttgatggtgccagttttgaggcctacaaatttgagttctcctgtaatgttgatgagacatattcaggatttgtactactagtcgagatgaaacttgatgatgacgtgggaaatgctgaagtggatctctacttaattgcaaagatggtcaaagcttacgtttcttcatgtggaaaggtgcatctcaatgcagatcagataaaggaagccatgtgctttcaggaatttttctttaatggtttgtttgggagattgtttcttggatccgaagcaaaaggcacagaaagaaaatttctacttcagactgaaataaaatcactatggtgctcatcgtacagctatttacttttgccgactgagacatctgacagttcaaataagaaatcttggaacatagattggatgggaatctgttcctgtgctaatgtggtggattttcttaggaaacactattccaatggtcttgagaatttcaaaggcggtagaggaaatatattactttctaggactggttcatccatgacagattgcaaggccgaaaatttagtccagtttgctaatagttcaattgatgtcaatgatcttgaagatatggtagtgttggctattcacacaggaagaatctactctgttattgaagttgttagtagcatttctgctgagagtcccttggaagaaaatgctgatgctgcctcatcagattatacttatgttgagtacttcaaaacaacgtcaattctagaagcactcacaacacttagatgctgtgaaaatttttcattggagcgtttggagttgcttggagattcagttctcaaatatgctgtgagctcctacctctttcttaaatatcccgagaaacatgaaggacaattaactgctcgacgaacaagagctatttgcaactcaaacctgcacaaactaggaacgaaaaacaagttacagggatacatacgagacagtgcatttgaaccacgacgttgggctgctccagggcagctttctagattcccagatccatgtcaatgcggagtggatactttggaagtgcccttggacagtagatttcaaactgaaaaggctgttaaggttggaaaattctgtgataaaggtcacagatggatgaactcaaaaaccattgctgattgtgttgaggccctcataggagcttattatgttggtggaggattgcttgctgcacttcatttgatgaagtggctcagcattgattctgaaaccggaccctcatttgctgatgaagccattacaagggcatcacttcgatcctacaatcccaaaactaatgaacttgctaacttggagttgaagcttagctatgaattttcagtcaagggcttgttacatgaggccatcacacatgcttctcagcaaaaactagactctgtgttggatctgctaactacacagcatctctatctcagccacacagatattgatcctggagtgttgactgatttgcggtcagctgctgttagtaatgaaaactttgctcaagctgctattagacaaaaccttcatccacatcttcaacattgctctgggttacttcggagtcacgtaaccgagtatgagaggttgcttacagaagctgagcacagtaccagctcagtggaagacattaagtgtccaaaggctcttggtgacatggtggaaagtatagcaggagctatactaattgatacaaagcttaatcttgatgaagtatggagggtttttaaaccaatattgtctccaattgtgaccccttctagtcttcaattgcatccgctacgtaaactgtctgaattatgtgattcacttgggtactttgtgaaagaaacatgtactaaagatgatacactagtaaatgtggaactaagtttgcagctggaagatgttcttttggttggaaacggcttagaccggagtagaaaagctgcgaaacataaggcagctcgtcaagtgttgaaggcgctcgaggttagttattatggtacttctcttctaagtgcctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1170

Amino Acids

131.63

Weight (kDa)

6.47

Isoelectric Point (pI)

37.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	198 - 301	1.8e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	372 - 456	2e-21	Dicer dimerisation domain
Ribonuclease_3	PF00636	761 - 894	1.5e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	761 - 818	2e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	966 - 1067	4.1e-14	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1409
AasI	GACNNNNNNGTC	1 cut(s)	1115
Acc36I	ACCTGC	2 cut(s)	1409, 2418
AccB1I	GGYRCC	1 cut(s)	1505
AciI	CCGC	6 cut(s)	304, 1241, 1977, 2526, 2977, 3284
AcoI	YGGCCR	1 cut(s)	211
AcuI	CTGAAG	3 cut(s)	1174, 1635, 1781
AfiI	CCNNNNNNNGG	1 cut(s)	2766
AflIII	ACRYGT	2 cut(s)	568, 3338
AhlI	ACTAGT	3 cut(s)	1255, 1576, 3358
AjiI	CACGTC	1 cut(s)	1603
AjnI	CCWGG	4 cut(s)	291, 1150, 2488, 2956
AjuI	GAANNNNNNNTTGG	2 cut(s)	740, 772
AleI	CACNNNNGTG	1 cut(s)	1819
AloI	GAACNNNNNNTCC	2 cut(s)	2292, 2324
Alw21I	GWGCWC	4 cut(s)	904, 1826, 2330, 3114
Alw26I	GTCTC	8 cut(s)	11, 894, 954, 1149, 1547, 1850, 2448, 3252
AlwNI	CAGNNNCTG	3 cut(s)	1424, 2987, 3107
Ama87I	CYCGRG	3 cut(s)	891, 2351, 3473
AoxI	GGCC	7 cut(s)	211, 237, 1175, 1518, 2031, 2652, 2865
ArsI	GACNNNNNNTTYG	6 cut(s)	953, 985, 2028, 2060, 2447, 2479
Asp700I	GAANNNNTTC	3 cut(s)	92, 1909, 3393
AspA2I	CCTAGG	1 cut(s)	1172
AspLEI	GCGC	1 cut(s)	3472
AspS9I	GGNCC	3 cut(s)	238, 2653, 2738
AsuHPI	GGTGA	1 cut(s)	3170
AsuII	TTCGAA	1 cut(s)	96
AvaI	CYCGRG	3 cut(s)	891, 2351, 3473
AvaII	GGWCC	1 cut(s)	2738
AvrII	CCTAGG	1 cut(s)	1172
BaeGI	GKGCMC	1 cut(s)	2550
BaeI	ACNNNNGTAYC	2 cut(s)	3345, 3378
BalI	TGGCCA	1 cut(s)	213
BamHI	GGATCC	1 cut(s)	1760
BanI	GGYRCC	1 cut(s)	1505
BanII	GRGCYC	1 cut(s)	2330
BbsI	GAAGAC	3 cut(s)	420, 3138, 3263
Bbv12I	GWGCWC	4 cut(s)	904, 1826, 2330, 3114
BbvCI	CCTCAGC	1 cut(s)	445
BccI	CCATC	7 cut(s)	972, 1022, 1496, 1636, 1894, 2610, 2876
BceAI	ACGGC	1 cut(s)	3427
BciT130I	CCWGG	4 cut(s)	293, 1152, 2490, 2958
BciVI	GTATCC	2 cut(s)	2438, 2527
BclI	TGATCA	1 cut(s)	1348
BcoDI	GTCTC	8 cut(s)	11, 894, 954, 1149, 1547, 1850, 2448, 3252
BcuI	ACTAGT	3 cut(s)	1255, 1576, 3358
BfmI	CTRYAG	1 cut(s)	1416
BfoI	RGCGCY	1 cut(s)	3473
BfuAI	ACCTGC	2 cut(s)	1409, 2418
BfuI	GTATCC	2 cut(s)	2438, 2527
BlnI	CCTAGG	1 cut(s)	1172
BlpI	GCTNAGC	3 cut(s)	2717, 2828, 3108
BmcAI	AGTACT	2 cut(s)	992, 2216
Bme1390I	CCNGG	4 cut(s)	293, 1152, 2490, 2958
Bme18I	GGWCC	1 cut(s)	2738
BmeT110I	CYCGRG	3 cut(s)	891, 2351, 3473
BmgBI	CACGTC	1 cut(s)	1603
BmgT120I	GGNCC	3 cut(s)	238, 2653, 2738
BmiI	GGNNCC	4 cut(s)	240, 1507, 1762, 2740
BmrFI	CCNGG	4 cut(s)	293, 1152, 2490, 2958
BpiI	GAAGAC	3 cut(s)	420, 3138, 3263
BplI	GAGNNNNNCTC	2 cut(s)	951, 983
BpmI	CTGGAG	5 cut(s)	1089, 1110, 1173, 2472, 2979
Bpu10I	CCTNAGC	1 cut(s)	445
Bpu1102I	GCTNAGC	3 cut(s)	2717, 2828, 3108
Bpu14I	TTCGAA	1 cut(s)	96
BpuEI	CTTGAG	2 cut(s)	1982, 2994
BsaAI	YACGTR	2 cut(s)	3076, 3290
BsaBI	GATNNNNATC	6 cut(s)	40, 170, 543, 1350, 1904, 2952
BsaI	GGTCTC	1 cut(s)	954
BsaJI	CCNNGG	5 cut(s)	1150, 1172, 2166, 2489, 2550
BsaWI	WCCGGW	4 cut(s)	704, 849, 2735, 3420
BsaXI	ACNNNNNCTCC	6 cut(s)	1083, 1113, 1216, 1246, 2292, 2322
Bsc4I	CCNNNNNNNGG	1 cut(s)	2766
Bse118I	RCCGGY	1 cut(s)	368
Bse1I	ACTGG	5 cut(s)	142, 1093, 1509, 2011, 2047
Bse3DI	GCAATG	4 cut(s)	180, 1226, 2634, 3049
Bse8I	GATNNNNATC	6 cut(s)	40, 170, 543, 1350, 1904, 2952
BseBI	CCWGG	4 cut(s)	293, 1152, 2490, 2958
BseDI	CCNNGG	5 cut(s)	1150, 1172, 2166, 2489, 2550
BseJI	GATNNNNATC	6 cut(s)	40, 170, 543, 1350, 1904, 2952
BseLI	CCNNNNNNNGG	1 cut(s)	2766
BseMI	GCAATG	4 cut(s)	180, 1226, 2634, 3049
BseMII	CTCAG	8 cut(s)	459, 1845, 2148, 2731, 2897, 2950, 3099, 3138
BseNI	ACTGG	5 cut(s)	142, 1093, 1509, 2011, 2047
BseRI	GAGGAG	1 cut(s)	827
BseSI	GKGCMC	1 cut(s)	2550
BseYI	CCCAGC	1 cut(s)	143
BsgI	GTGCAG	2 cut(s)	2396, 2679
BshFI	GGCC	7 cut(s)	213, 239, 1177, 1520, 2033, 2654, 2867
BshNI	GGYRCC	1 cut(s)	1505
BsiHKAI	GWGCWC	4 cut(s)	904, 1826, 2330, 3114
BsiHKCI	CYCGRG	3 cut(s)	891, 2351, 3473
BsiSI	CCGG	5 cut(s)	369, 705, 850, 2736, 3421
BslFI	GGGAC	4 cut(s)	53, 909, 956, 2148
BslI	CCNNNNNNNGG	1 cut(s)	2766
BsmAI	GTCTC	8 cut(s)	11, 894, 954, 1149, 1547, 1850, 2448, 3252
BsmFI	GGGAC	4 cut(s)	53, 909, 956, 2148
BsmI	GAATGC	3 cut(s)	313, 359, 1464
BsnI	GGCC	7 cut(s)	213, 239, 1177, 1520, 2033, 2654, 2867
Bso31I	GGTCTC	1 cut(s)	954
BsoBI	CYCGRG	3 cut(s)	891, 2351, 3473
Bsp119I	TTCGAA	1 cut(s)	96
Bsp1286I	GDGCHC	5 cut(s)	904, 1826, 2330, 2550, 3114
Bsp1720I	GCTNAGC	3 cut(s)	2717, 2828, 3108
BspACI	CCGC	6 cut(s)	304, 1241, 1977, 2526, 2977, 3284
BspANI	GGCC	7 cut(s)	213, 239, 1177, 1520, 2033, 2654, 2867
BspCNI	CTCAG	8 cut(s)	458, 1846, 2149, 2730, 2896, 2949, 3100, 3137
BspHI	TCATGA	1 cut(s)	273
BspLI	GGNNCC	4 cut(s)	240, 1507, 1762, 2740
BspMAI	CTGCAG	1 cut(s)	1420
BspMI	ACCTGC	2 cut(s)	1409, 2418
BspQI	GCTCTTC	1 cut(s)	426
BspT104I	TTCGAA	1 cut(s)	96
BspT107I	GGYRCC	1 cut(s)	1505
BspTNI	GGTCTC	1 cut(s)	954
BsrDI	GCAATG	4 cut(s)	180, 1226, 2634, 3049
BsrFI	RCCGGY	1 cut(s)	368
BsrI	ACTGG	5 cut(s)	142, 1093, 1509, 2011, 2047
BssAI	RCCGGY	1 cut(s)	368
BssECI	CCNNGG	5 cut(s)	1150, 1172, 2166, 2489, 2550
BssT1I	CCWWGG	3 cut(s)	1172, 2166, 2550
Bst2UI	CCWGG	4 cut(s)	293, 1152, 2490, 2958
Bst6I	CTCTTC	2 cut(s)	426, 3504
BstBAI	YACGTR	2 cut(s)	3076, 3290
BstBI	TTCGAA	1 cut(s)	96
BstC8I	GCNNGC	1 cut(s)	2691
BstH2I	RGCGCY	1 cut(s)	3473
BstHHI	GCGC	1 cut(s)	3472
BstMAI	GTCTC	8 cut(s)	11, 894, 954, 1149, 1547, 1850, 2448, 3252
BstMWI	GCNNNNNNNGC	4 cut(s)	1091, 2186, 2756, 3014
BstNI	CCWGG	4 cut(s)	293, 1152, 2490, 2958
BstNSI	RCATGY	4 cut(s)	440, 572, 2879, 3342
BstSCI	CCNGG	4 cut(s)	291, 1150, 2488, 2956
BstSFI	CTRYAG	1 cut(s)	1416
BstSLI	GKGCMC	1 cut(s)	2550
BstSNI	TACGTA	1 cut(s)	3290
BstV2I	GAAGAC	3 cut(s)	420, 3138, 3263
BstX2I	RGATCY	5 cut(s)	1021, 1621, 1760, 2512, 2908
BstXI	CCANNNNNNTGG	2 cut(s)	2478, 3127
BstYI	RGATCY	5 cut(s)	1021, 1621, 1760, 2512, 2908
BsuI	GTATCC	2 cut(s)	2438, 2527
BsuRI	GGCC	7 cut(s)	213, 239, 1177, 1520, 2033, 2654, 2867
BtrI	CACGTC	1 cut(s)	1603
BtsI	GCAGTG	2 cut(s)	9, 132
BtsIMutI	CAGTG	7 cut(s)	9, 132, 778, 798, 1220, 2464, 3132
BveI	ACCTGC	2 cut(s)	1409, 2418
Cac8I	GCNNGC	1 cut(s)	2691
CaiI	CAGNNNCTG	3 cut(s)	1424, 2987, 3107
CciI	TCATGA	1 cut(s)	273
CfoI	GCGC	1 cut(s)	3472
Cfr10I	RCCGGY	1 cut(s)	368
Cfr13I	GGNCC	3 cut(s)	238, 2653, 2738
CspCI	CAANNNNNGTGG	2 cut(s)	703, 738
DraI	TTTAAA	1 cut(s)	3235
DrdI	GACNNNNNNGTC	1 cut(s)	1115
DseDI	GACNNNNNNGTC	1 cut(s)	1115
EaeI	YGGCCR	1 cut(s)	211
Eam1104I	CTCTTC	2 cut(s)	426, 3504
EarI	CTCTTC	2 cut(s)	426, 3504
Ecl136II	GAGCTC	1 cut(s)	2328
Eco105I	TACGTA	1 cut(s)	3290
Eco130I	CCWWGG	3 cut(s)	1172, 2166, 2550
Eco147I	AGGCCT	1 cut(s)	1520
Eco24I	GRGCYC	1 cut(s)	2330
Eco31I	GGTCTC	1 cut(s)	954
Eco32I	GATATC	1 cut(s)	55
Eco47I	GGWCC	1 cut(s)	2738
Eco53kI	GAGCTC	1 cut(s)	2328
Eco57I	CTGAAG	3 cut(s)	1174, 1635, 1781
Eco88I	CYCGRG	3 cut(s)	891, 2351, 3473
EcoICRI	GAGCTC	1 cut(s)	2328
EcoO109I	RGGNCCY	1 cut(s)	2653
EcoRI	GAATTC	1 cut(s)	819
EcoRII	CCWGG	4 cut(s)	291, 1150, 2488, 2956
EcoRV	GATATC	1 cut(s)	55
EcoT14I	CCWWGG	3 cut(s)	1172, 2166, 2550
EcoT22I	ATGCAT	3 cut(s)	63, 313, 1072
EcoT38I	GRGCYC	1 cut(s)	2330
ErhI	CCWWGG	3 cut(s)	1172, 2166, 2550
FalI	AAGNNNNNCTT	6 cut(s)	1362, 1394, 1468, 1500, 3381, 3413
FaqI	GGGAC	4 cut(s)	53, 909, 956, 2148
FbaI	TGATCA	1 cut(s)	1348
FriOI	GRGCYC	1 cut(s)	2330
GlaI	GCGC	1 cut(s)	3471
GsaI	CCCAGC	1 cut(s)	147
GsuI	CTGGAG	5 cut(s)	1089, 1110, 1173, 2472, 2979
HaeII	RGCGCY	1 cut(s)	3473
HaeIII	GGCC	7 cut(s)	213, 239, 1177, 1520, 2033, 2654, 2867
HapII	CCGG	5 cut(s)	369, 705, 850, 2736, 3421
HhaI	GCGC	1 cut(s)	3472
Hin6I	GCGC	1 cut(s)	3470
HinP1I	GCGC	1 cut(s)	3470
HincII	GTYRAC	1 cut(s)	2967
HindII	GTYRAC	1 cut(s)	2967
HindIII	AAGCTT	6 cut(s)	516, 1303, 1311, 1650, 2825, 3203
HpaII	CCGG	5 cut(s)	369, 705, 850, 2736, 3421
HphI	GGTGA	1 cut(s)	3170
Hpy166II	GTNNAC	2 cut(s)	2967, 3293
Hpy8I	GTNNAC	2 cut(s)	2967, 3293
Hpy99I	CGWCG	2 cut(s)	2385, 2478
HpyCH4IV	ACGT	6 cut(s)	1602, 1656, 2228, 2476, 3075, 3289
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1091, 2186, 2756, 3014
HpySE526I	ACGT	6 cut(s)	1602, 1656, 2228, 2476, 3075, 3289
HspAI	GCGC	1 cut(s)	3470
Ksp22I	TGATCA	1 cut(s)	1348
LguI	GCTCTTC	1 cut(s)	426
LmnI	GCTCC	8 cut(s)	842, 1091, 1152, 2281, 2333, 2491, 2663, 3182
MaeII	ACGT	6 cut(s)	1602, 1656, 2228, 2476, 3075, 3289
MfeI	CAATTG	3 cut(s)	2064, 3252, 3275
MflI	RGATCY	5 cut(s)	1021, 1621, 1760, 2512, 2908
MhlI	GDGCHC	5 cut(s)	904, 1826, 2330, 2550, 3114
MlsI	TGGCCA	1 cut(s)	213
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	5 cut(s)	13, 1103, 2170, 2891, 3079
MmeI	TCCRAC	4 cut(s)	111, 2569, 2886, 3385
Mox20I	TGGCCA	1 cut(s)	213
Mph1103I	ATGCAT	3 cut(s)	63, 313, 1072
MroXI	GAANNNNTTC	3 cut(s)	92, 1909, 3393
MscI	TGGCCA	1 cut(s)	213
MslI	CAYNNNNRTG	4 cut(s)	1683, 1819, 2257, 2521
Msp20I	TGGCCA	1 cut(s)	213
MspA1I	CMGCKG	2 cut(s)	2984, 3385
MspI	CCGG	5 cut(s)	369, 705, 850, 2736, 3421
MspR9I	CCNGG	4 cut(s)	293, 1152, 2490, 2958
MunI	CAATTG	3 cut(s)	2064, 3252, 3275
Mva1269I	GAATGC	3 cut(s)	313, 359, 1464
MvaI	CCWGG	4 cut(s)	293, 1152, 2490, 2958
MwoI	GCNNNNNNNGC	4 cut(s)	1091, 2186, 2756, 3014
NlaIV	GGNNCC	4 cut(s)	240, 1507, 1762, 2740
NmuCI	GTSAC	4 cut(s)	2609, 3071, 3158, 3256
NsiI	ATGCAT	3 cut(s)	63, 313, 1072
NspI	RCATGY	4 cut(s)	440, 572, 2879, 3342
NspV	TTCGAA	1 cut(s)	96
OliI	CACNNNNGTG	1 cut(s)	1819
PaeR7I	CTCGAG	1 cut(s)	3473
PagI	TCATGA	1 cut(s)	273
PaqCI	CACCTGC	1 cut(s)	1409
PceI	AGGCCT	1 cut(s)	1520
PciI	ACATGT	2 cut(s)	568, 3338
PciSI	GCTCTTC	1 cut(s)	426
PctI	GAATGC	3 cut(s)	313, 359, 1464
PdmI	GAANNNNTTC	3 cut(s)	92, 1909, 3393
PfeI	GAWTC	8 cut(s)	761, 914, 1905, 2118, 2303, 2505, 2726, 3311
PfoI	TCCNGGA	2 cut(s)	291, 2956
PleI	GAGTC	5 cut(s)	12, 1103, 2169, 2891, 3078
PpsI	GAGTC	5 cut(s)	12, 1103, 2169, 2891, 3078
Ppu21I	YACGTR	2 cut(s)	3076, 3290
PscI	ACATGT	2 cut(s)	568, 3338
Psp124BI	GAGCTC	1 cut(s)	2330
Psp6I	CCWGG	4 cut(s)	291, 1150, 2488, 2956
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	4 cut(s)	291, 1150, 2488, 2956
PspN4I	GGNNCC	4 cut(s)	240, 1507, 1762, 2740
PspPI	GGNCC	3 cut(s)	238, 2653, 2738
PspXI	VCTCGAGB	1 cut(s)	3473
PstI	CTGCAG	1 cut(s)	1420
PstNI	CAGNNNCTG	3 cut(s)	1424, 2987, 3107
PsuI	RGATCY	5 cut(s)	1021, 1621, 1760, 2512, 2908
PvuII	CAGCTG	2 cut(s)	2984, 3385
RseI	CAYNNNNRTG	4 cut(s)	1683, 1819, 2257, 2521
SacI	GAGCTC	1 cut(s)	2330
SapI	GCTCTTC	1 cut(s)	426
Sau96I	GGNCC	3 cut(s)	238, 2653, 2738
ScaI	AGTACT	2 cut(s)	992, 2216
SchI	GAGTC	5 cut(s)	13, 1103, 2170, 2891, 3079
ScrFI	CCNGG	4 cut(s)	293, 1152, 2490, 2958
SduI	GDGCHC	5 cut(s)	904, 1826, 2330, 2550, 3114
SfcI	CTRYAG	1 cut(s)	1416
Sfr274I	CTCGAG	1 cut(s)	3473
SfuI	TTCGAA	1 cut(s)	96
SinI	GGWCC	1 cut(s)	2738
SlaI	CTCGAG	1 cut(s)	3473
SmiMI	CAYNNNNRTG	4 cut(s)	1683, 1819, 2257, 2521
SmlI	CTYRAG	3 cut(s)	1961, 3009, 3473
SmoI	CTYRAG	3 cut(s)	1961, 3009, 3473
SnaBI	TACGTA	1 cut(s)	3290
SpeI	ACTAGT	3 cut(s)	1255, 1576, 3358
SseBI	AGGCCT	1 cut(s)	1520
SsiI	CCGC	6 cut(s)	304, 1241, 1977, 2526, 2977, 3284
SspI	AATATT	1 cut(s)	3243
SstI	GAGCTC	1 cut(s)	2330
StuI	AGGCCT	1 cut(s)	1520
StyD4I	CCNGG	4 cut(s)	291, 1150, 2488, 2956
StyI	CCWWGG	3 cut(s)	1172, 2166, 2550
TaiI	ACGT	6 cut(s)	1605, 1659, 2231, 2479, 3078, 3292
TaqI	TCGA	8 cut(s)	96, 1010, 1018, 1368, 1581, 2380, 2779, 3474
TatI	WGTACW	4 cut(s)	990, 1570, 2214, 3341
TauI	GCSGC	1 cut(s)	1244
TfiI	GAWTC	8 cut(s)	761, 914, 1905, 2118, 2303, 2505, 2726, 3311
TscAI	CASTG	7 cut(s)	16, 132, 778, 805, 1220, 2464, 3132
TseFI	GTSAC	4 cut(s)	2609, 3071, 3158, 3256
Tsp45I	GTSAC	4 cut(s)	2609, 3071, 3158, 3256
TspRI	CASTG	7 cut(s)	16, 132, 778, 805, 1220, 2464, 3132
VpaK11BI	GGWCC	1 cut(s)	2738
XbaI	TCTAGA	2 cut(s)	2236, 2501
XceI	RCATGY	4 cut(s)	440, 572, 2879, 3342
XhoI	CTCGAG	1 cut(s)	3473
XmaJI	CCTAGG	1 cut(s)	1172
XmnI	GAANNNNTTC	3 cut(s)	92, 1909, 3393
ZrmI	AGTACT	2 cut(s)	992, 2216
Zsp2I	ATGCAT	3 cut(s)	63, 313, 1072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.