Rmu_ssc0000024.1_g000014

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000024.1
OG0005002
Physical Location & Seq
Forward (+)
68585 .. 70036
1452 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
atgattcgcgttccaaagaggacgtccgcattttcgctaaagtgcggacgacggcgctgcagcccaactcaggccacgacggcgcgacgcggcttgcaggagggaggccaggggtcgtgcggaggggtctgcgttgtgaagagtttaaagtatgacaagtcgcatgagtgggcgagagttgatgggatgttggctacaatagggttaagtgatcctgctcagaagcacttgggtggtattattatccatgttgaactgcctgatgtcggtgaagatgtagcacaaggcaccagatgtggtactgttgagtctgtcaaagccagcaagggcctccactctcctctctctggcaatgtagttgaagtaaatcaaaagcttcgtacctctcctgctttggtgaatagaagcccgtatgatgatggatggatcatgaaggtagaaatgagcaacaaagaggagctgaataatctgatggactatgatgagtacgcgagctatgtgaaaaccaagttgccaaacattgatgaaccagttgaaaacacttag

Protein Analysis

181

Amino Acids

19.77

Weight (kDa)

8.25

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 49 - 169 2.6e-39 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 4 cut(s) 9, 85, 90, 491
AciI CCGC 4 cut(s) 27, 45, 90, 120
AclWI GGATC 2 cut(s) 206, 434
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 3 cut(s) 301, 382, 488
AfiI CCNNNNNNNGG 3 cut(s) 70, 266, 347
AgsI TTSAA 3 cut(s) 254, 362, 536
AjnI CCWGG 1 cut(s) 108
AleI CACNNNNGTG 1 cut(s) 231
AluBI AGCT 3 cut(s) 376, 460, 495
AluI AGCT 3 cut(s) 376, 460, 495
AlwI GGATC 2 cut(s) 206, 434
AoxI GGCC 3 cut(s) 72, 106, 328
ApeKI GCWGC 2 cut(s) 57, 60
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AspLEI GCGC 2 cut(s) 57, 85
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 2 cut(s) 281, 409
BanI GGYRCC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 44, 72
BccI CCATC 4 cut(s) 176, 413, 417, 466
BceAI ACGGC 2 cut(s) 68, 96
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BciT130I CCWGG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 58
BfoI RGCGCY 1 cut(s) 58
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 3 cut(s) 58, 61, 91
BlsI GCNGC 3 cut(s) 59, 62, 92
Bme1390I CCNGG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 266, 347
Bse1I ACTGG 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 2 cut(s) 192, 428
BseLI CCNNNNNNNGG 3 cut(s) 70, 266, 347
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 2 cut(s) 83, 233
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 2 cut(s) 330, 470
BseXI GCAGC 2 cut(s) 44, 72
Bsh1236I CGCG 4 cut(s) 9, 85, 90, 491
BshFI GGCC 3 cut(s) 74, 108, 330
BshNI GGYRCC 1 cut(s) 287
BslI CCNNNNNNNGG 3 cut(s) 70, 266, 347
BsnI GGCC 3 cut(s) 74, 108, 330
Bsp143I GATC 2 cut(s) 211, 426
BspACI CCGC 4 cut(s) 27, 45, 90, 120
BspANI GGCC 3 cut(s) 74, 108, 330
BspCNI CTCAG 2 cut(s) 82, 232
BspFNI CGCG 4 cut(s) 9, 85, 90, 491
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 62
BspPI GGATC 2 cut(s) 206, 434
BspT107I GGYRCC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 358
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 2 cut(s) 211, 426
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 3 cut(s) 95, 322, 493
BstDEI CTNAG 3 cut(s) 69, 219, 543
BstF5I GGATG 2 cut(s) 192, 428
BstFNI CGCG 4 cut(s) 9, 85, 90, 491
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 2 cut(s) 57, 85
BstKTI GATC 2 cut(s) 214, 429
BstMBI GATC 2 cut(s) 211, 426
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 58
BstUI CGCG 4 cut(s) 9, 85, 90, 491
BstV1I GCAGC 2 cut(s) 44, 72
BsuRI GGCC 3 cut(s) 74, 108, 330
BtsCI GGATG 2 cut(s) 192, 428
Cac8I GCNNGC 3 cut(s) 95, 322, 493
CciI TCATGA 1 cut(s) 429
CfoI GCGC 2 cut(s) 57, 85
Cfr13I GGNCC 1 cut(s) 328
CseI GACGC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 300, 381, 487
CviAII CATG 3 cut(s) 164, 248, 430
CviQI GTAC 3 cut(s) 300, 381, 487
DdeI CTNAG 3 cut(s) 69, 219, 543
DpnI GATC 2 cut(s) 213, 428
DpnII GATC 2 cut(s) 211, 426
DraI TTTAAA 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 108
FaeI CATG 3 cut(s) 167, 251, 433
FaiI YATR 7 cut(s) 153, 165, 249, 414, 431, 480, 498
FatI CATG 3 cut(s) 163, 247, 429
Fnu4HI GCNGC 3 cut(s) 58, 61, 91
FokI GGATG 2 cut(s) 199, 435
Fsp4HI GCNGC 3 cut(s) 58, 61, 91
GlaI GCGC 2 cut(s) 56, 84
GluI GCNGC 3 cut(s) 58, 61, 91
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 3 cut(s) 74, 108, 330
HgaI GACGC 1 cut(s) 96
HhaI GCGC 2 cut(s) 57, 85
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 167, 251, 433
Hin6I GCGC 2 cut(s) 55, 83
HinP1I GCGC 2 cut(s) 55, 83
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 4, 308
HphI GGTGA 2 cut(s) 281, 409
Hpy188I TCNGA 2 cut(s) 222, 471
Hpy188III TCNNGA 1 cut(s) 430
Hpy99I CGWCG 3 cut(s) 54, 82, 90
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 60, 97
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 3 cut(s) 69, 219, 543
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 167, 251, 433
HspAI GCGC 2 cut(s) 55, 83
Kzo9I GATC 2 cut(s) 211, 426
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 2 cut(s) 44, 72
MaeII ACGT 1 cut(s) 23
MalI GATC 2 cut(s) 213, 428
MboI GATC 2 cut(s) 211, 426
MboII GAAGA 2 cut(s) 151, 284
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 8 cut(s) 12, 94, 98, 116, 341, 351, 394, 448
MseI TTAA 2 cut(s) 146, 206
MslI CAYNNNNRTG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 110
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 4 cut(s) 9, 85, 90, 491
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 2 cut(s) 211, 426
NlaIII CATG 3 cut(s) 167, 251, 433
NlaIV GGNNCC 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 231
PagI TCATGA 1 cut(s) 429
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 3 cut(s) 59, 62, 92
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 328
PstI CTGCAG 1 cut(s) 62
RsaI GTAC 3 cut(s) 301, 382, 488
RsaNI GTAC 3 cut(s) 300, 381, 487
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 146, 206
SatI GCNGC 3 cut(s) 58, 61, 91
Sau3AI GATC 2 cut(s) 211, 426
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 110
SetI ASST 6 cut(s) 26, 378, 386, 438, 462, 497
SfcI CTRYAG 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 231
SsiI CCGC 4 cut(s) 27, 45, 90, 120
StyD4I CCNGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 26
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 146, 206
Tru9I TTAA 2 cut(s) 146, 206
TseI GCWGC 2 cut(s) 57, 60
TspDTI ATGAA 2 cut(s) 446, 540
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.