Rmu_ssc0000075.1_g000016

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000075.1
OG0003589
Physical Location & Seq
Reverse (-)
76508 .. 78164
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
atgaatttggctaatccaaaacaagcctcaaggcttaagcacgttgatacgataaaatcaaatcaaatgccggcagcagcagcatcaatcacaccaataccaaatttccatttccattcccacaaggtagctcccattaggtttcgtctctgggcctcgattctcagcattgccgccgtagccaaccgagccaccatggtcaccactgccgccaccagcaacagctgtcgcggtggcgcggatcctcgtgctactcacaattgctgggcatacaaggtgggagatcagtaccggtctaatgatgacggtgagccatctggaacggccgggaagcccattcattctgctattgagtcttcaggagttgatagagtaatggtggtggtcatccgttattttggaggaattaaattggggactggaggactagtcagggcatatggaggagtaacatcggaatgcttgagaaatgccccaactcatctcgtaaaatcgaaggtaagaatgggtgtagaggttccatttgatcttatcggcattctataccatcagctacaatcttttcaagttgaagacatcaaacaagattatgagaccggtaaagatggtatcaccatggttacctttcaagttgattttgactgtgttgataaattagaggatgctatcaaaaataactgtagccgggagttagtgttctataagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

235

Amino Acids

25.71

Weight (kDa)

8.94

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 80 - 155 2.6e-28 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 231, 239
AciI CCGC 4 cut(s) 174, 210, 231, 239
AclWI GGATC 2 cut(s) 236, 249
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 2 cut(s) 4, 103
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 290
AflII CTTAAG 1 cut(s) 35
AgeI ACCGGT 2 cut(s) 291, 596
AgsI TTSAA 3 cut(s) 566, 572, 629
AhlI ACTAGT 1 cut(s) 427
AloI GAACNNNNNNTCC 1 cut(s) 680
AluBI AGCT 3 cut(s) 131, 225, 553
AluI AGCT 3 cut(s) 131, 225, 553
Alw26I GTCTC 2 cut(s) 152, 587
AlwI GGATC 2 cut(s) 236, 249
AoxI GGCC 2 cut(s) 153, 324
ApeKI GCWGC 3 cut(s) 74, 77, 80
ApoI RAATTY 2 cut(s) 4, 103
AsiGI ACCGGT 2 cut(s) 291, 596
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 2 cut(s) 328, 686
AsuHPI GGTGA 3 cut(s) 193, 320, 604
BamHI GGATCC 1 cut(s) 241
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 2 cut(s) 348, 579
BbvI GCAGC 3 cut(s) 86, 89, 92
BccI CCATC 3 cut(s) 322, 555, 599
BceAI ACGGC 2 cut(s) 161, 339
BcnI CCSGG 2 cut(s) 328, 686
BcoDI GTCTC 2 cut(s) 152, 587
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 679
BfrI CTTAAG 1 cut(s) 35
BisI GCNGC 5 cut(s) 75, 78, 81, 174, 210
BlsI GCNGC 5 cut(s) 76, 79, 82, 175, 211
Bme1390I CCNGG 2 cut(s) 328, 686
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 243, 519
BmrFI CCNGG 2 cut(s) 328, 686
BmsI GCATC 2 cut(s) 92, 652
BpiI GAAGAC 2 cut(s) 348, 579
BpmI CTGGAG 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 13, 484
BpuMI CCSGG 2 cut(s) 328, 686
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 195, 615
BsaWI WCCGGW 2 cut(s) 291, 596
BsaXI ACNNNNNCTCC 3 cut(s) 414, 444, 680
Bse118I RCCGGY 3 cut(s) 70, 291, 596
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 195, 615
BseGI GGATG 2 cut(s) 387, 667
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 1 cut(s) 459
BseX3I CGGCCG 1 cut(s) 324
BseXI GCAGC 3 cut(s) 86, 89, 92
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 2 cut(s) 231, 239
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 155, 326
BshTI ACCGGT 2 cut(s) 291, 596
BsiEI CGRYCG 1 cut(s) 327
BsiSI CCGG 5 cut(s) 71, 292, 327, 597, 685
BslFI GGGAC 1 cut(s) 430
BsmAI GTCTC 2 cut(s) 152, 587
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 2 cut(s) 464, 537
BsnI GGCC 2 cut(s) 155, 326
Bso31I GGTCTC 1 cut(s) 587
Bsp143I GATC 3 cut(s) 241, 283, 526
Bsp19I CCATGG 2 cut(s) 195, 615
BspACI CCGC 4 cut(s) 174, 210, 231, 239
BspANI GGCC 2 cut(s) 155, 326
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 2 cut(s) 231, 239
BspLI GGNNCC 2 cut(s) 243, 519
BspPI GGATC 2 cut(s) 236, 249
BspTI CTTAAG 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 3 cut(s) 70, 291, 596
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 3 cut(s) 70, 291, 596
BssECI CCNNGG 2 cut(s) 195, 615
BssMI GATC 3 cut(s) 241, 283, 526
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 195, 615
Bst2BI CACGAG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 308, 644, 680
BstAFI CTTAAG 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 164
BstDSI CCRYGG 2 cut(s) 195, 615
BstEII GGTNACC 2 cut(s) 199, 619
BstF5I GGATG 2 cut(s) 387, 667
BstFNI CGCG 2 cut(s) 231, 239
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 3 cut(s) 244, 286, 529
BstMAI GTCTC 2 cut(s) 152, 587
BstMBI GATC 3 cut(s) 241, 283, 526
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 3 cut(s) 80, 179, 188
BstPI GGTNACC 2 cut(s) 199, 619
BstSCI CCNGG 2 cut(s) 326, 684
BstSFI CTRYAG 1 cut(s) 679
BstUI CGCG 2 cut(s) 231, 239
BstV1I GCAGC 3 cut(s) 86, 89, 92
BstV2I GAAGAC 2 cut(s) 348, 579
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BstZI CGGCCG 1 cut(s) 324
BsuRI GGCC 2 cut(s) 155, 326
BtgI CCRYGG 2 cut(s) 195, 615
BtsCI GGATG 2 cut(s) 387, 667
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 72
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 3 cut(s) 70, 291, 596
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 289
CspAI ACCGGT 2 cut(s) 291, 596
CviAII CATG 2 cut(s) 196, 616
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 164
DpnI GATC 3 cut(s) 243, 285, 528
DpnII GATC 3 cut(s) 241, 283, 526
EaeI YGGCCR 1 cut(s) 324
EagI CGGCCG 1 cut(s) 324
EclXI CGGCCG 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 195, 615
Eco31I GGTCTC 1 cut(s) 587
Eco52I CGGCCG 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 342
Eco91I GGTNACC 2 cut(s) 199, 619
EcoO65I GGTNACC 2 cut(s) 199, 619
EcoT14I CCWWGG 2 cut(s) 195, 615
ErhI CCWWGG 2 cut(s) 195, 615
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 2 cut(s) 199, 619
FaiI YATR 8 cut(s) 197, 271, 439, 441, 544, 591, 617, 702
FaqI GGGAC 1 cut(s) 430
FatI CATG 2 cut(s) 195, 615
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 5 cut(s) 75, 78, 81, 174, 210
FokI GGATG 2 cut(s) 374, 674
Fsp4HI GCNGC 5 cut(s) 75, 78, 81, 174, 210
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 238
GluI GCNGC 5 cut(s) 75, 78, 81, 174, 210
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 2 cut(s) 155, 326
HapII CCGG 5 cut(s) 71, 292, 327, 597, 685
HhaI GCGC 1 cut(s) 239
Hin1II CATG 2 cut(s) 199, 619
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HinfI GANTC 2 cut(s) 160, 353
HpaII CCGG 5 cut(s) 71, 292, 327, 597, 685
HphI GGTGA 3 cut(s) 193, 320, 604
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 2 cut(s) 318, 360
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 3 cut(s) 308, 644, 680
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 179, 188
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 199, 619
HspAI GCGC 1 cut(s) 237
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 241, 283, 526
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 3 cut(s) 86, 89, 92
LweI GCATC 2 cut(s) 92, 652
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 3 cut(s) 199, 448, 619
MalI GATC 3 cut(s) 243, 285, 528
MboI GATC 3 cut(s) 241, 283, 526
MboII GAAGA 2 cut(s) 348, 584
MfeI CAATTG 1 cut(s) 259
MflI RGATCY 1 cut(s) 241
MluCI AATT 6 cut(s) 4, 103, 259, 405, 410, 653
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 8 cut(s) 37, 166, 255, 395, 416, 437, 508, 652
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 36, 408
MslI CAYNNNNRTG 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 225
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 5 cut(s) 71, 292, 327, 597, 685
MspR9I CCNGG 2 cut(s) 328, 686
MunI CAATTG 1 cut(s) 259
Mva1269I GAATGC 2 cut(s) 464, 537
MvnI CGCG 2 cut(s) 231, 239
MwoI GCNNNNNNNGC 3 cut(s) 80, 179, 188
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 2 cut(s) 328, 686
NcoI CCATGG 2 cut(s) 195, 615
NdeI CATATG 1 cut(s) 439
NdeII GATC 3 cut(s) 241, 283, 526
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 2 cut(s) 199, 619
NlaIV GGNNCC 2 cut(s) 243, 519
NmuCI GTSAC 1 cut(s) 199
PctI GAATGC 2 cut(s) 464, 537
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 160
PinAI ACCGGT 2 cut(s) 291, 596
PkrI GCNGC 5 cut(s) 76, 79, 82, 175, 211
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
PspEI GGTNACC 2 cut(s) 199, 619
PspFI CCCAGC 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 243, 519
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 457
SaqAI TTAA 2 cut(s) 36, 408
SatI GCNGC 5 cut(s) 75, 78, 81, 174, 210
Sau3AI GATC 3 cut(s) 241, 283, 526
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 328, 686
SfaNI GCATC 2 cut(s) 92, 652
SfcI CTRYAG 1 cut(s) 679
SmiMI CAYNNNNRTG 1 cut(s) 457
SmlI CTYRAG 3 cut(s) 28, 35, 463
SmoI CTYRAG 3 cut(s) 28, 35, 463
SpeI ACTAGT 1 cut(s) 427
Sse9I AATT 6 cut(s) 4, 103, 259, 405, 410, 653
SsiI CCGC 4 cut(s) 174, 210, 231, 239
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 326, 684
StyI CCWWGG 2 cut(s) 195, 615
TaaI ACNGT 3 cut(s) 308, 644, 680
TaiI ACGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 158, 494
TasI AATT 6 cut(s) 4, 103, 259, 405, 410, 653
TauI GCSGC 2 cut(s) 176, 212
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 36, 408
Tru9I TTAA 2 cut(s) 36, 408
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 3 cut(s) 74, 77, 80
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 17, 329
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 211
Vha464I CTTAAG 1 cut(s) 35
XapI RAATTY 2 cut(s) 4, 103
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.