Rmu_ssc0000126.1_g000065

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000126.1
OG0004858
Physical Location & Seq
Forward (+)
309019 .. 313958
4940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2256 bp
atggcaacaaccgaaccgattgaactcgaattcaaaaggtacttctccggtggcgttgagcttcaaatgtccggtgaggcgcgtcgccttcacccttctctcaaggtacactattatcacactctcaactcctcccgacccacctctgattttcacagcacctttgattttctcactcagttcttgccattctcttctatcaaacccaattcccaatgctctataaactccaatgagcggcgtaaagtcactgttgggttatccaggatcataaagaaccaacagggctatgtactgaagggcttttccagaaccttctgcccattttttcttgtaaagattatgaaactgttctgttgtagagaaactgcatttgggttctttaaactagcctttagggatgataatcagcagactgtggagtcatgctgtgtggctgcacatgttttggcggccgaaaatctacggtttctggctcaagatgtggtttcgtgtgtgattgggaggattggcccgagtaggagtagggatttggtggggtttatgtggggagggcattgtgaatacgagtcggatttttcggttcttgatacactaatgagggggttcttgaatgcggaaatgggcgccgaggctttggaggttttgaggaggatgagggaggtgggagtgaggccgagtttgtcagcggttgcgattctgttcaggttgttgataagggttggtgattatggtagtgtgtggaaggtgtttagggatatgattagagaagggccttatccatgtaattatgtttttagtgcaatgattcttgggttttgtcaaaaagggttggttagagttggggagagtttgttacaggtgatgtggaaattccaatgtgaaccggatgtttttgcttataacattgtgatctatgcaaattgtgcgagagggcagacagctgatgcgcttgaatgggtggagttgatgattgcgaggggatgtaaaccgagcattgttacatttaatacagtcataagtgcattgtgtaaggagggaaatgtggtggaggcaaggaagctttttgatgatgtcccagacatgggtgtttatccgaataccataatttacaagctcatctactattctactttgtcaaattttaaatttctattattagatattcattttctgcttaaagtttctgttagtatcccgatggatcaaaaacttgaatccgaaagcagagtttgcgtcacgggagcttctggctttctggcatcttggcttattaagcgacttctcatgtcaggatatcatgtaactggaactgtcagagatccaggaaaccagaagaaattggcacatttgtggaggctggaaggagctaaagaaagactgagattggtgaaggctgatttagttgaagaaagtagctttgatgatgcaatcttcgggtgccatggtgtcttccactctgcttctcctgtacccaaactttcatctgatccaaagaaagaaatcctagaccccgctattgaaggcacgttgaatgtgcttcgttcatgtaagaagaatccatctctaaggcgtgtggttctcacctcatcttcttcagctgtaagggtgagagatggtgatgattttgaccccaatattccattggatgagtcatcttggagctctgtggaactctgccagaaactgcaggcaatgttgagaaagttgaggcagggtctcagcaatgctacccttctagtatggaattcgatactggtgtggatgatcctaggactcctacgcgctgagagggcagcattggatttctgcaaagcaaatgggatcgatttagtaaccgttctacccacattcgtcgttggacctagtttgccacctgatttatgttctactgcatctgatatacttggcctgctcaagggtgaaacagagaggttcaaattgcatggaagaatgggatatgttcacatcgatgatgttgcactttgccacatcctagtttatgagcacaaaagtgctcatggtcgatacatttgcaactcgacagtgcttgacaacaatgagttagcatccttgctatccacaagatatccttccatacctatccctgaaaggtttgagcaactggaaaggccacactatgatttcaacacctcaaagatgagggctctaggatttaagttcaagacagtccaagagatgtttgatgattgcattgccgctctggtggagcaaggccatctttctccattctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

751

Amino Acids

84.59

Weight (kDa)

8.41

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 261 - 309 2.8e-06 PPR repeat family
PPR_3 PF13812 286 - 343 1.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 296 - 345 1.1e-12 PPR repeat family
PPR_1 PF12854 327 - 358 6.6e-12 PPR repeat
PPR_2 PF13041 331 - 369 1.7e-09 PPR repeat family
PPR PF01535 334 - 364 7.2e-07 PPR repeat
Epimerase PF01370 411 - 534 1e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 413 - 536 6.6e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 413 - 532 6.1e-09 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 413 - 544 1.7e-07 Male sterility protein
NAD_binding_10 PF13460 415 - 539 4.7e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AasI GACNNNNNNGTC 1 cut(s) 421
AccB1I GGYRCC 2 cut(s) 626, 1440
AccBSI CCGCTC 2 cut(s) 238, 2222
AccII CGCG 2 cut(s) 82, 1785
AciI CCGC 6 cut(s) 238, 452, 617, 689, 1515, 2220
AclWI GGATC 6 cut(s) 275, 1212, 1316, 1484, 1762, 1832
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 5 cut(s) 29, 872, 1141, 1148, 1747
AcuI CTGAAG 2 cut(s) 317, 1581
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 4 cut(s) 41, 108, 294, 1473
AfiI CCNNNNNNNGG 4 cut(s) 237, 1084, 1085, 1918
AflIII ACRYGT 1 cut(s) 442
AjnI CCWGG 2 cut(s) 263, 1324
AjuI GAANNNNNNNTTGG 2 cut(s) 357, 389
AleI CACNNNNGTG 2 cut(s) 1351, 2013
AluBI AGCT 9 cut(s) 61, 944, 1063, 1117, 1247, 1370, 1419, 1601, 1665
AluI AGCT 9 cut(s) 61, 944, 1063, 1117, 1247, 1370, 1419, 1601, 1665
Alw21I GWGCWC 3 cut(s) 1667, 2010, 2020
Alw26I GTCTC 1 cut(s) 1724
AlwI GGATC 6 cut(s) 275, 1212, 1316, 1484, 1762, 1832
AlwNI CAGNNNCTG 1 cut(s) 1687
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 7 cut(s) 453, 511, 674, 773, 1909, 2132, 2236
ApeKI GCWGC 2 cut(s) 437, 1796
ApoI RAATTY 5 cut(s) 29, 872, 1141, 1148, 1747
Asp700I GAANNNNTTC 1 cut(s) 350
AspA2I CCTAGG 1 cut(s) 1771
AspLEI GCGC 4 cut(s) 82, 629, 952, 1787
AspS9I GGNCC 3 cut(s) 512, 773, 1862
AsuHPI GGTGA 9 cut(s) 83, 86, 737, 874, 1402, 1576, 1621, 1631, 1934
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 1 cut(s) 1862
AvrII CCTAGG 1 cut(s) 1771
BaeI ACNNNNGTAYC 2 cut(s) 1745, 1778
BanI GGYRCC 2 cut(s) 626, 1440
BanII GRGCYC 2 cut(s) 1667, 2170
BbsI GAAGAC 1 cut(s) 1444
Bbv12I GWGCWC 3 cut(s) 1667, 2010, 2020
BbvI GCAGC 2 cut(s) 424, 1808
BccI CCATC 4 cut(s) 1195, 1570, 1610, 2247
BcgI CGANNNNNNTGC 4 cut(s) 1961, 1995, 2016, 2050
BciT130I CCWGG 2 cut(s) 265, 1326
BciVI GTATCC 1 cut(s) 1205
BcoDI GTCTC 1 cut(s) 1724
BfaI CTAG 8 cut(s) 389, 1508, 1739, 1772, 1866, 1997, 2171, 2254
BfmI CTRYAG 1 cut(s) 1688
BfoI RGCGCY 1 cut(s) 630
BfuI GTATCC 1 cut(s) 1205
BisI GCNGC 5 cut(s) 239, 438, 453, 1797, 2220
BlnI CCTAGG 1 cut(s) 1771
BlsI GCNGC 5 cut(s) 240, 439, 454, 1798, 2221
Bme1390I CCNGG 2 cut(s) 265, 1326
Bme18I GGWCC 1 cut(s) 1862
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 3 cut(s) 512, 773, 1862
BmiI GGNNCC 2 cut(s) 628, 1442
BmrFI CCNGG 2 cut(s) 265, 1326
BmsI GCATC 5 cut(s) 937, 1271, 1417, 1904, 2078
BpiI GAAGAC 1 cut(s) 1444
BpuEI CTTGAG 3 cut(s) 86, 462, 1901
Bsa29I ATCGAT 2 cut(s) 1827, 1971
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 1724
BsaJI CCNNGG 3 cut(s) 630, 1444, 1771
BsaWI WCCGGW 3 cut(s) 47, 71, 886
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 1084, 1085, 1918
Bse1I ACTGG 3 cut(s) 1312, 1761, 2130
Bse3DI GCAATG 4 cut(s) 810, 1701, 1732, 2214
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 2 cut(s) 265, 1326
BseCI ATCGAT 2 cut(s) 1827, 1971
BseDI CCNNGG 3 cut(s) 630, 1444, 1771
BseGI GGATG 8 cut(s) 406, 660, 895, 989, 1654, 1770, 1992, 2069
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 4 cut(s) 237, 1084, 1085, 1918
BseMI GCAATG 4 cut(s) 810, 1701, 1732, 2214
BseMII CTCAG 4 cut(s) 191, 1373, 1735, 1779
BseNI ACTGG 3 cut(s) 1312, 1761, 2130
BseRI GAGGAG 2 cut(s) 121, 664
BseX3I CGGCCG 1 cut(s) 453
BseXI GCAGC 2 cut(s) 424, 1808
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 2 cut(s) 82, 1785
Bsh1285I CGRYCG 1 cut(s) 456
BshFI GGCC 7 cut(s) 455, 513, 676, 775, 1911, 2134, 2238
BshNI GGYRCC 2 cut(s) 626, 1440
BshVI ATCGAT 2 cut(s) 1827, 1971
BsiEI CGRYCG 1 cut(s) 456
BsiHKAI GWGCWC 3 cut(s) 1667, 2010, 2020
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 3 cut(s) 48, 72, 887
BslFI GGGAC 1 cut(s) 1061
BslI CCNNNNNNNGG 4 cut(s) 237, 1084, 1085, 1918
BsmAI GTCTC 1 cut(s) 1724
BsmFI GGGAC 1 cut(s) 1061
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 7 cut(s) 455, 513, 676, 775, 1911, 2134, 2238
Bso31I GGTCTC 1 cut(s) 1724
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 4 cut(s) 1667, 2010, 2020, 2170
Bsp143I GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
Bsp19I CCATGG 1 cut(s) 1444
BspACI CCGC 6 cut(s) 238, 452, 617, 689, 1515, 2220
BspANI GGCC 7 cut(s) 455, 513, 676, 775, 1911, 2134, 2238
BspCNI CTCAG 4 cut(s) 190, 1374, 1734, 1780
BspDI ATCGAT 2 cut(s) 1827, 1971
BspFNI CGCG 2 cut(s) 82, 1785
BspLI GGNNCC 2 cut(s) 628, 1442
BspMAI CTGCAG 1 cut(s) 1692
BspPI GGATC 6 cut(s) 275, 1212, 1316, 1484, 1762, 1832
BspT107I GGYRCC 2 cut(s) 626, 1440
BspTNI GGTCTC 1 cut(s) 1724
BsrBI CCGCTC 2 cut(s) 238, 2222
BsrDI GCAATG 4 cut(s) 810, 1701, 1732, 2214
BsrI ACTGG 3 cut(s) 1312, 1761, 2130
BssECI CCNNGG 3 cut(s) 630, 1444, 1771
BssMI GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
BssNI GRCGYC 1 cut(s) 627
BssT1I CCWWGG 2 cut(s) 1444, 1771
Bst2UI CCWGG 2 cut(s) 265, 1326
Bst4CI ACNGT 9 cut(s) 253, 351, 418, 468, 1015, 1315, 1840, 2047, 2191
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 627
BstAPI GCANNNNNTGC 2 cut(s) 926, 1233
BstC8I GCNNGC 2 cut(s) 1692, 1913
BstDEI CTNAG 5 cut(s) 177, 1382, 1568, 1721, 1788
BstDSI CCRYGG 1 cut(s) 1444
BstENI CCTNNNNNAGG 1 cut(s) 1916
BstF5I GGATG 8 cut(s) 406, 660, 895, 989, 1654, 1770, 1992, 2069
BstFNI CGCG 2 cut(s) 82, 1785
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 4 cut(s) 82, 629, 952, 1787
BstKTI GATC 7 cut(s) 270, 915, 1207, 1324, 1492, 1770, 1827
BstMAI GTCTC 1 cut(s) 1724
BstMBI GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
BstMCI CGRYCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 4 cut(s) 926, 1233, 1276, 1793
BstNI CCWGG 2 cut(s) 265, 1326
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 263, 1324
BstSFI CTRYAG 1 cut(s) 1688
BstUI CGCG 2 cut(s) 82, 1785
BstV1I GCAGC 2 cut(s) 424, 1808
BstV2I GAAGAC 1 cut(s) 1444
BstX2I RGATCY 1 cut(s) 1321
BstYI RGATCY 1 cut(s) 1321
BstZI CGGCCG 1 cut(s) 453
Bsu15I ATCGAT 2 cut(s) 1827, 1971
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 7 cut(s) 455, 513, 676, 775, 1911, 2134, 2238
BsuTUI ATCGAT 2 cut(s) 1827, 1971
BtgI CCRYGG 1 cut(s) 1444
BtsCI GGATG 8 cut(s) 406, 660, 895, 989, 1654, 1770, 1992, 2069
BtsIMutI CAGTG 2 cut(s) 249, 2052
Cac8I GCNNGC 2 cut(s) 1692, 1913
CaiI CAGNNNCTG 1 cut(s) 1687
CfoI GCGC 4 cut(s) 82, 629, 952, 1787
Cfr13I GGNCC 3 cut(s) 512, 773, 1862
ClaI ATCGAT 2 cut(s) 1827, 1971
CseI GACGC 2 cut(s) 71, 1225
Csp6I GTAC 4 cut(s) 40, 107, 293, 1472
CviQI GTAC 4 cut(s) 40, 107, 293, 1472
DdeI CTNAG 5 cut(s) 177, 1382, 1568, 1721, 1788
DinI GGCGCC 1 cut(s) 628
DpnI GATC 7 cut(s) 269, 914, 1206, 1323, 1491, 1769, 1826
DpnII GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
DraI TTTAAA 2 cut(s) 385, 1147
DrdI GACNNNNNNGTC 1 cut(s) 421
DseDI GACNNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 453
EagI CGGCCG 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 1665
EclXI CGGCCG 1 cut(s) 453
Eco130I CCWWGG 2 cut(s) 1444, 1771
Eco24I GRGCYC 2 cut(s) 1667, 2170
Eco31I GGTCTC 1 cut(s) 1724
Eco32I GATATC 2 cut(s) 1298, 2090
Eco47I GGWCC 1 cut(s) 1862
Eco52I CGGCCG 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 1665
Eco57I CTGAAG 2 cut(s) 317, 1581
Eco88I CYCGRG 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 1665
EcoNI CCTNNNNNAGG 1 cut(s) 1916
EcoO109I RGGNCCY 1 cut(s) 773
EcoRI GAATTC 2 cut(s) 29, 1747
EcoRII CCWGG 2 cut(s) 263, 1324
EcoRV GATATC 2 cut(s) 1298, 2090
EcoT14I CCWWGG 2 cut(s) 1444, 1771
EcoT38I GRGCYC 2 cut(s) 1667, 2170
EgeI GGCGCC 1 cut(s) 628
EheI GGCGCC 1 cut(s) 628
ErhI CCWWGG 2 cut(s) 1444, 1771
FalI AAGNNNNNCTT 3 cut(s) 2077, 2109, 2226
FaqI GGGAC 1 cut(s) 1061
FauI CCCGC 1 cut(s) 1522
Fnu4HI GCNGC 5 cut(s) 239, 438, 453, 1797, 2220
FokI GGATG 8 cut(s) 413, 667, 902, 996, 1661, 1777, 1979, 2056
FriOI GRGCYC 2 cut(s) 1667, 2170
Fsp4HI GCNGC 5 cut(s) 239, 438, 453, 1797, 2220
FspBI CTAG 8 cut(s) 389, 1508, 1739, 1772, 1866, 1997, 2171, 2254
GlaI GCGC 4 cut(s) 81, 628, 951, 1786
GluI GCNGC 5 cut(s) 239, 438, 453, 1797, 2220
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 7 cut(s) 455, 513, 676, 775, 1911, 2134, 2238
HapII CCGG 3 cut(s) 48, 72, 887
HgaI GACGC 2 cut(s) 71, 1225
HhaI GCGC 4 cut(s) 82, 629, 952, 1787
Hin1I GRCGYC 1 cut(s) 627
Hin6I GCGC 4 cut(s) 80, 627, 950, 1785
HinP1I GCGC 4 cut(s) 80, 627, 950, 1785
HindIII AAGCTT 1 cut(s) 1061
HinfI GANTC 8 cut(s) 422, 569, 697, 808, 1217, 1558, 1652, 1776
HpaII CCGG 3 cut(s) 48, 72, 887
HphI GGTGA 9 cut(s) 83, 86, 737, 874, 1402, 1576, 1621, 1631, 1934
Hpy166II GTNNAC 4 cut(s) 109, 884, 989, 1966
Hpy188I TCNGA 7 cut(s) 148, 574, 1098, 1222, 1319, 1489, 1900
Hpy188III TCNNGA 8 cut(s) 135, 309, 479, 587, 610, 1198, 1293, 2185
Hpy8I GTNNAC 4 cut(s) 109, 884, 989, 1966
Hpy99I CGWCG 2 cut(s) 87, 1859
HpyCH4III ACNGT 9 cut(s) 253, 351, 418, 468, 1015, 1315, 1840, 2047, 2191
HpyCH4IV ACGT 1 cut(s) 1529
HpyF10VI GCNNNNNNNGC 4 cut(s) 926, 1233, 1276, 1793
HpyF3I CTNAG 5 cut(s) 177, 1382, 1568, 1721, 1788
HpySE526I ACGT 1 cut(s) 1529
Hsp92I GRCGYC 1 cut(s) 627
HspAI GCGC 4 cut(s) 80, 627, 950, 1785
KasI GGCGCC 1 cut(s) 626
Kzo9I GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
LmnI GCTCC 4 cut(s) 1244, 1367, 1662, 2230
Lsp1109I GCAGC 2 cut(s) 424, 1808
LweI GCATC 5 cut(s) 937, 1271, 1417, 1904, 2078
MaeI CTAG 8 cut(s) 389, 1508, 1739, 1772, 1866, 1997, 2171, 2254
MaeII ACGT 1 cut(s) 1529
MaeIII GTNAC 6 cut(s) 247, 855, 1000, 1237, 1303, 1834
MalI GATC 7 cut(s) 269, 914, 1206, 1323, 1491, 1769, 1826
MbiI CCGCTC 2 cut(s) 238, 2222
MboI GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
MboII GAAGA 9 cut(s) 186, 1348, 1421, 1426, 1444, 1567, 1584, 1587, 1962
MflI RGATCY 1 cut(s) 1321
MhlI GDGCHC 4 cut(s) 1667, 2010, 2020, 2170
Mly113I GGCGCC 1 cut(s) 627
MlyI GAGTC 4 cut(s) 431, 578, 1661, 1770
MmeI TCCRAC 2 cut(s) 552, 1840
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 6 cut(s) 384, 1008, 1146, 1179, 1275, 2178
MslI CAYNNNNRTG 3 cut(s) 1351, 1971, 2013
MspA1I CMGCKG 3 cut(s) 689, 944, 1601
MspI CCGG 3 cut(s) 48, 72, 887
MspR9I CCNGG 2 cut(s) 265, 1326
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 2 cut(s) 265, 1326
MvnI CGCG 2 cut(s) 82, 1785
MwoI GCNNNNNNNGC 4 cut(s) 926, 1233, 1276, 1793
NarI GGCGCC 1 cut(s) 627
NcoI CCATGG 1 cut(s) 1444
NdeII GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
NlaIV GGNNCC 2 cut(s) 628, 1442
NmeAIII GCCGAG 2 cut(s) 655, 702
NmuCI GTSAC 2 cut(s) 247, 1237
NspI RCATGY 1 cut(s) 446
OliI CACNNNNGTG 2 cut(s) 1351, 2013
PciI ACATGT 1 cut(s) 442
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 697, 808, 1217, 1558
PfoI TCCNGGA 2 cut(s) 263, 1324
PkrI GCNGC 5 cut(s) 240, 439, 454, 1798, 2221
PleI GAGTC 4 cut(s) 430, 577, 1660, 1770
PluTI GGCGCC 1 cut(s) 630
PpsI GAGTC 4 cut(s) 430, 577, 1660, 1770
PscI ACATGT 1 cut(s) 442
PsiI TTATAA 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 1667
Psp6I CCWGG 2 cut(s) 263, 1324
PspGI CCWGG 2 cut(s) 263, 1324
PspN4I GGNNCC 2 cut(s) 628, 1442
PspPI GGNCC 3 cut(s) 512, 773, 1862
PstI CTGCAG 1 cut(s) 1692
PstNI CAGNNNCTG 1 cut(s) 1687
PsuI RGATCY 1 cut(s) 1321
PvuII CAGCTG 2 cut(s) 944, 1601
RsaI GTAC 4 cut(s) 41, 108, 294, 1473
RsaNI GTAC 4 cut(s) 40, 107, 293, 1472
RseI CAYNNNNRTG 3 cut(s) 1351, 1971, 2013
SacI GAGCTC 1 cut(s) 1667
SaqAI TTAA 6 cut(s) 384, 1008, 1146, 1179, 1275, 2178
SatI GCNGC 5 cut(s) 239, 438, 453, 1797, 2220
Sau3AI GATC 7 cut(s) 267, 912, 1204, 1321, 1489, 1767, 1824
Sau96I GGNCC 3 cut(s) 512, 773, 1862
SchI GAGTC 4 cut(s) 431, 578, 1661, 1770
ScrFI CCNGG 2 cut(s) 265, 1326
SduI GDGCHC 4 cut(s) 1667, 2010, 2020, 2170
SfaNI GCATC 5 cut(s) 937, 1271, 1417, 1904, 2078
SfcI CTRYAG 1 cut(s) 1688
SfoI GGCGCC 1 cut(s) 628
SinI GGWCC 1 cut(s) 1862
SmiMI CAYNNNNRTG 3 cut(s) 1351, 1971, 2013
SmlI CTYRAG 3 cut(s) 101, 477, 1916
SmoI CTYRAG 3 cut(s) 101, 477, 1916
SsiI CCGC 6 cut(s) 238, 452, 617, 689, 1515, 2220
SspDI GGCGCC 1 cut(s) 626
SspI AATATT 1 cut(s) 1639
SspMI CTAG 8 cut(s) 389, 1508, 1739, 1772, 1866, 1997, 2171, 2254
SstI GAGCTC 1 cut(s) 1667
StyD4I CCNGG 2 cut(s) 263, 1324
StyI CCWWGG 2 cut(s) 1444, 1771
TaaI ACNGT 9 cut(s) 253, 351, 418, 468, 1015, 1315, 1840, 2047, 2191
TaiI ACGT 1 cut(s) 1532
TaqI TCGA 6 cut(s) 27, 1751, 1827, 1971, 2026, 2042
TatI WGTACW 1 cut(s) 292
TauI GCSGC 3 cut(s) 241, 455, 2222
TfiI GAWTC 4 cut(s) 697, 808, 1217, 1558
Tru1I TTAA 6 cut(s) 384, 1008, 1146, 1179, 1275, 2178
Tru9I TTAA 6 cut(s) 384, 1008, 1146, 1179, 1275, 2178
TscAI CASTG 2 cut(s) 256, 2052
TseFI GTSAC 2 cut(s) 247, 1237
TseI GCWGC 2 cut(s) 437, 1796
Tsp45I GTSAC 2 cut(s) 247, 1237
TspDTI ATGAA 4 cut(s) 359, 1157, 1473, 1536
TspRI CASTG 2 cut(s) 256, 2052
VpaK11BI GGWCC 1 cut(s) 1862
XagI CCTNNNNNAGG 1 cut(s) 1916
XapI RAATTY 5 cut(s) 29, 872, 1141, 1148, 1747
XceI RCATGY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 1642
XmaJI CCTAGG 1 cut(s) 1771
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 8 cut(s) 389, 1508, 1739, 1772, 1866, 1997, 2171, 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.