Rmu_ssc0000330.1_g000006

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000330.1
OG0008903
Physical Location & Seq
Reverse (-)
32083 .. 35950
3868 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
atgatcgacgttctccgtgcagctaggttggagttccagttcttgcagcaagcttggaggtcgctctatccatcaccggcgacgatttcgtgctgcaacctaaacttcgattctggtgtgcctgcctatgtgtctctcgagctccccccatctggaaatcaaatagcaccgttgagctgggtttctcacagtgcaagtctacaaccggctaagaaggaagaagagaatggagagcagccggatctctcacagtaccgtgggagggtgccggtgaccggtgatcggattcaagtgaaagttggccggaatcaggcgacatgtgaccggattcctgcaaaatttggtcggaatcctgcgaccagtgacaggattcaagctgccggtgtaaacgagcaaggtggagtggagattaaggtggacaaagtggatttccgatcactggcaagagaagacaaagaacccgtaaaggagaaggtggaaatgcttcatgagatcaacgacggaggagcaaattctggaaatacaggggttgccggctttgtgggtaaaatagctaccgcagttgcagatacctttcaatcaaccaaggatactcgagattgtcccggcgttcgtgacgagcggtccgattccatcgccggggattttggttatagcacgtgcagctccatcgtcgggaatctaaatgaacgatcgggtttagaaactcgacaagaactcgccgttgaagttgatcgacgcggtgcggaatctggttctcaaaggcttgcattcgtcttggactggaaaaatatgcacgatgagattgtattacgttacctaatattcattgaatattatttcttgattcaagatacaacggaaaatagatgtggacgtcgatccgacccggttgaggaaattcggatcgacctggtctttctagttcatcgtgaacaccatgactctctcttcggcgcaacacgagttgagcaagccatccatgaagttcgatatgccggagaagctgaccgccgtcaatttctcggcgaagaaccgatccaggtccttgatgaggatcaccgacttgctcgtggtctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

40.2

Weight (kDa)

5.22

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 199
AccII CGCG 1 cut(s) 741
AciI CCGC 5 cut(s) 558, 622, 741, 746, 1012
AclWI GGATC 5 cut(s) 249, 876, 914, 1033, 1065
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 3 cut(s) 338, 511, 900
AcvI CACGTG 1 cut(s) 660
AcyI GRCGYC 1 cut(s) 877
AfaI GTAC 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 317
AgeI ACCGGT 1 cut(s) 275
AgsI TTSAA 6 cut(s) 290, 374, 578, 728, 833, 851
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 2 cut(s) 912, 1041
AloI GAACNNNNNNTCC 2 cut(s) 23, 55
AluBI AGCT 8 cut(s) 23, 53, 142, 177, 377, 554, 666, 1007
AluI AGCT 8 cut(s) 23, 53, 142, 177, 377, 554, 666, 1007
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 5 cut(s) 249, 876, 914, 1033, 1065
Ama87I CYCGRG 2 cut(s) 137, 594
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 6 cut(s) 20, 46, 93, 235, 377, 663
ApoI RAATTY 3 cut(s) 338, 511, 900
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AsiGI ACCGGT 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 959
AspS9I GGNCC 2 cut(s) 624, 1045
AsuC2I CCSGG 3 cut(s) 606, 640, 890
AsuHPI GGTGA 4 cut(s) 66, 283, 290, 1052
AvaI CYCGRG 2 cut(s) 137, 594
AvaII GGWCC 2 cut(s) 624, 1045
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 144
BauI CACGAG 2 cut(s) 963, 1071
BbrPI CACGTG 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 6 cut(s) 32, 58, 80, 247, 364, 675
BccI CCATC 5 cut(s) 79, 157, 641, 677, 986
BceAI ACGGC 2 cut(s) 707, 999
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 2 cut(s) 914, 1043
BciVI GTATCC 1 cut(s) 583
BcnI CCSGG 3 cut(s) 606, 640, 890
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 2 cut(s) 24, 923
BfuI GTATCC 1 cut(s) 583
BisI GCNGC 6 cut(s) 21, 47, 94, 236, 378, 664
BlsI GCNGC 6 cut(s) 22, 48, 95, 237, 379, 665
Bme1390I CCNGG 5 cut(s) 606, 640, 890, 914, 1043
Bme18I GGWCC 2 cut(s) 624, 1045
BmeRI GACNNNNNGTC 1 cut(s) 622
BmeT110I CYCGRG 2 cut(s) 137, 594
BmgT120I GGNCC 2 cut(s) 624, 1045
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 5 cut(s) 606, 640, 890, 914, 1043
BoxI GACNNNNGTC 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 456
BpuMI CCSGG 3 cut(s) 606, 640, 890
BsaAI YACGTR 1 cut(s) 660
BsaBI GATNNNNATC 1 cut(s) 1056
BsaHI GRCGYC 1 cut(s) 877
BsaJI CCNNGG 3 cut(s) 256, 585, 639
BsaWI WCCGGW 2 cut(s) 275, 324
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bse118I RCCGGY 6 cut(s) 76, 205, 268, 275, 380, 533
Bse1I ACTGG 4 cut(s) 37, 360, 444, 788
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 2 cut(s) 914, 1043
BseDI CCNNGG 3 cut(s) 256, 585, 639
BseGI GGATG 1 cut(s) 978
BseJI GATNNNNATC 1 cut(s) 1056
BseNI ACTGG 4 cut(s) 37, 360, 444, 788
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 6 cut(s) 32, 58, 80, 247, 364, 675
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 2 cut(s) 39, 682
Bsh1236I CGCG 1 cut(s) 741
Bsh1285I CGRYCG 1 cut(s) 695
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 265
BshTI ACCGGT 1 cut(s) 275
BsiEI CGRYCG 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 2 cut(s) 137, 594
BslFI GGGAC 1 cut(s) 588
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 1 cut(s) 303
BsoBI CYCGRG 2 cut(s) 137, 594
Bsp1286I GDGCHC 1 cut(s) 144
BspACI CCGC 5 cut(s) 558, 622, 741, 746, 1012
BspANI GGCC 1 cut(s) 303
BspFNI CGCG 1 cut(s) 741
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 5 cut(s) 249, 876, 914, 1033, 1065
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 622
BsrFI RCCGGY 6 cut(s) 76, 205, 268, 275, 380, 533
BsrI ACTGG 4 cut(s) 37, 360, 444, 788
BssAI RCCGGY 6 cut(s) 76, 205, 268, 275, 380, 533
BssECI CCNNGG 3 cut(s) 256, 585, 639
BssNI GRCGYC 1 cut(s) 877
BssSI CACGAG 2 cut(s) 963, 1071
BssT1I CCWWGG 1 cut(s) 585
Bst2BI CACGAG 2 cut(s) 963, 1071
Bst2UI CCWGG 2 cut(s) 914, 1043
Bst4CI ACNGT 4 cut(s) 171, 191, 252, 257
Bst6I CTCTTC 2 cut(s) 216, 956
BstACI GRCGYC 1 cut(s) 877
BstBAI YACGTR 1 cut(s) 660
BstC8I GCNNGC 5 cut(s) 51, 123, 535, 768, 975
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 256
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 1052
BstF5I GGATG 1 cut(s) 978
BstFNI CGCG 1 cut(s) 741
BstHHI GCGC 1 cut(s) 959
BstMAI GTCTC 1 cut(s) 138
BstMCI CGRYCG 1 cut(s) 695
BstMWI GCNNNNNNNGC 2 cut(s) 663, 1004
BstNI CCWGG 2 cut(s) 914, 1043
BstNSI RCATGY 1 cut(s) 321
BstPAI GACNNNNGTC 1 cut(s) 1014
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 5 cut(s) 604, 638, 888, 912, 1041
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 6 cut(s) 32, 58, 80, 247, 364, 675
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuI GTATCC 1 cut(s) 583
BsuRI GGCC 1 cut(s) 303
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 619
BtsCI GGATG 1 cut(s) 978
BtsIMutI CAGTG 3 cut(s) 196, 367, 437
Cac8I GCNNGC 5 cut(s) 51, 123, 535, 768, 975
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 959
Cfr10I RCCGGY 6 cut(s) 76, 205, 268, 275, 380, 533
Cfr13I GGNCC 2 cut(s) 624, 1045
CpoI CGGWCCG 1 cut(s) 624
CseI GACGC 1 cut(s) 747
CsiI ACCWGGT 1 cut(s) 912
Csp6I GTAC 1 cut(s) 253
CspAI ACCGGT 1 cut(s) 275
CspI CGGWCCG 1 cut(s) 624
CviAII CATG 4 cut(s) 318, 488, 941, 983
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 1 cut(s) 210
DriI GACNNNNNGTC 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 216, 956
Eam1105I GACNNNNNGTC 1 cut(s) 622
EarI CTCTTC 2 cut(s) 216, 956
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 2 cut(s) 624, 1045
Eco53kI GAGCTC 1 cut(s) 142
Eco72I CACGTG 1 cut(s) 660
Eco88I CYCGRG 2 cut(s) 137, 594
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 142
EcoNI CCTNNNNNAGG 1 cut(s) 1052
EcoO109I RGGNCCY 1 cut(s) 1045
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 912, 1041
EcoT14I CCWWGG 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 4 cut(s) 321, 491, 944, 986
FaiI YATR 8 cut(s) 129, 319, 489, 654, 794, 942, 984, 996
FaqI GGGAC 1 cut(s) 588
FatI CATG 4 cut(s) 317, 487, 940, 982
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 21, 47, 94, 236, 378, 664
FokI GGATG 1 cut(s) 965
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 6 cut(s) 21, 47, 94, 236, 378, 664
FspBI CTAG 2 cut(s) 24, 923
GlaI GCGC 1 cut(s) 958
GluI GCNGC 6 cut(s) 21, 47, 94, 236, 378, 664
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 303
HgaI GACGC 1 cut(s) 747
HhaI GCGC 1 cut(s) 959
Hin1I GRCGYC 1 cut(s) 877
Hin1II CATG 4 cut(s) 321, 491, 944, 986
Hin6I GCGC 1 cut(s) 957
HinP1I GCGC 1 cut(s) 957
HindIII AAGCTT 1 cut(s) 51
HphI GGTGA 4 cut(s) 66, 283, 290, 1052
Hpy166II GTNNAC 5 cut(s) 200, 388, 418, 875, 935
Hpy188I TCNGA 6 cut(s) 285, 348, 434, 628, 886, 906
Hpy8I GTNNAC 5 cut(s) 200, 388, 418, 875, 935
Hpy99I CGWCG 6 cut(s) 11, 85, 503, 677, 741, 882
HpyAV CCTTC 2 cut(s) 208, 466
HpyCH4III ACNGT 4 cut(s) 171, 191, 252, 257
HpyCH4IV ACGT 4 cut(s) 9, 659, 814, 877
HpyCH4V TGCA 9 cut(s) 20, 46, 96, 194, 335, 566, 663, 770, 796
HpyF10VI GCNNNNNNNGC 2 cut(s) 663, 1004
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 4 cut(s) 9, 659, 814, 877
Hsp92I GRCGYC 1 cut(s) 877
Hsp92II CATG 4 cut(s) 321, 491, 944, 986
HspAI GCGC 1 cut(s) 957
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
LmnI GCTCC 3 cut(s) 147, 506, 671
Lsp1109I GCAGC 6 cut(s) 32, 58, 80, 247, 364, 675
MabI ACCWGGT 1 cut(s) 912
MaeI CTAG 2 cut(s) 24, 923
MaeII ACGT 4 cut(s) 9, 659, 814, 877
MaeIII GTNAC 5 cut(s) 271, 320, 362, 614, 815
MbiI CCGCTC 1 cut(s) 622
MboII GAAGA 5 cut(s) 230, 233, 461, 943, 1043
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 4 cut(s) 338, 511, 900, 1019
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 3 cut(s) 9, 326, 909
MnlI CCTC 5 cut(s) 51, 255, 497, 889, 1048
MroNI GCCGGC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 411
MspR9I CCNGG 5 cut(s) 606, 640, 890, 914, 1043
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 2 cut(s) 914, 1043
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 2 cut(s) 663, 1004
NaeI GCCGGC 1 cut(s) 535
NciI CCSGG 3 cut(s) 606, 640, 890
NgoMIV GCCGGC 1 cut(s) 533
NlaIII CATG 4 cut(s) 321, 491, 944, 986
NlaIV GGNNCC 1 cut(s) 267
NmeAIII GCCGAG 1 cut(s) 1005
NmuCI GTSAC 4 cut(s) 271, 320, 362, 614
NspI RCATGY 1 cut(s) 321
PaeR7I CTCGAG 2 cut(s) 137, 594
PagI TCATGA 1 cut(s) 487
PciI ACATGT 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 624
PctI GAATGC 1 cut(s) 770
PdiI GCCGGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 483
PflFI GACNNNGTC 1 cut(s) 914
PinAI ACCGGT 1 cut(s) 275
PkrI GCNGC 6 cut(s) 22, 48, 95, 237, 379, 665
Ple19I CGATCG 1 cut(s) 695
PleI GAGTC 1 cut(s) 938
PmaCI CACGTG 1 cut(s) 660
PmlI CACGTG 1 cut(s) 660
PpsI GAGTC 1 cut(s) 938
Ppu21I YACGTR 1 cut(s) 660
PpuMI RGGWCCY 1 cut(s) 1045
PscI ACATGT 1 cut(s) 317
PshAI GACNNNNGTC 1 cut(s) 1014
Psp124BI GAGCTC 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 2 cut(s) 912, 1041
PspCI CACGTG 1 cut(s) 660
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 2 cut(s) 912, 1041
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 2 cut(s) 624, 1045
PspPPI RGGWCCY 1 cut(s) 1045
PsuI RGATCY 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 914
PvuI CGATCG 1 cut(s) 695
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 624
RsrII CGGWCCG 1 cut(s) 624
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 6 cut(s) 21, 47, 94, 236, 378, 664
Sau96I GGNCC 2 cut(s) 624, 1045
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 5 cut(s) 606, 640, 890, 914, 1043
SduI GDGCHC 1 cut(s) 144
SexAI ACCWGGT 1 cut(s) 912
Sfr274I CTCGAG 2 cut(s) 137, 594
SgrAI CRCCGGYG 1 cut(s) 76
SinI GGWCC 2 cut(s) 624, 1045
SlaI CTCGAG 2 cut(s) 137, 594
SmlI CTYRAG 2 cut(s) 137, 594
SmoI CTYRAG 2 cut(s) 137, 594
Sse9I AATT 4 cut(s) 338, 511, 900, 1019
SsiI CCGC 5 cut(s) 558, 622, 741, 746, 1012
SspI AATATT 2 cut(s) 825, 836
SspMI CTAG 2 cut(s) 24, 923
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 5 cut(s) 604, 638, 888, 912, 1041
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 4 cut(s) 171, 191, 252, 257
TaiI ACGT 4 cut(s) 12, 662, 817, 880
TaqI TCGA 9 cut(s) 6, 108, 138, 595, 709, 736, 880, 909, 991
TasI AATT 4 cut(s) 338, 511, 900, 1019
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 196, 367, 444
TseFI GTSAC 4 cut(s) 271, 320, 362, 614
TseI GCWGC 6 cut(s) 20, 46, 93, 235, 377, 663
Tsp45I GTSAC 4 cut(s) 271, 320, 362, 614
TspDTI ATGAA 5 cut(s) 476, 702, 817, 917, 999
TspGWI ACGGA 3 cut(s) 5, 516, 875
TspRI CASTG 3 cut(s) 196, 367, 444
Tth111I GACNNNGTC 1 cut(s) 914
VpaK11BI GGWCC 2 cut(s) 624, 1045
XagI CCTNNNNNAGG 1 cut(s) 1052
XapI RAATTY 3 cut(s) 338, 511, 900
XceI RCATGY 1 cut(s) 321
XhoI CTCGAG 2 cut(s) 137, 594
XmiI GTMKAC 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 24, 923
ZraI GACGTC 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.