Rroxscaffold_1G00033380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003699
Physical Location & Seq
Reverse (-)
48178234 .. 48183758
5525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033380.1

Sequence Viewer

Length: 789 bp
ATGTCGGCTTTCCGATCTGAGAAATCATCCTCGTTCGGTGGAGCCCGAGCGCATGAGCTGGCCTCCGGCCATGCCGTCGTCATGGGGTCTGTTGCCGGACGGGTATTGATGGCTAATACTCTTGTTCCATCGAGATGGGGTTTGCTCGGGGCTTGTCGGCGTCAACAAAGCAAAGCTTCGCCAAGTTCTTTAGCAAGCTCGTGGAGAATCAACGGGCACCAAGCACACGTGTCGATTCATCCGTCATCAAAGCTAAAGAACACTCACCACGTGACACCGGTTTGTGGTTCAAAGCCGATCAAGAGCAAGCCTCTCGCATCCCTTGATCAACCGCCTTCTACAAGCCGTCAACAAAGAAAATCTTCTATCAAGTTCTTCATCAAGCTCGTGGAGAATCAACAAGCACCAAACACATGTGTCAGTTCAACCTTTACCAAAGCCAAAGAATGCTCACCACATGACACCATTCGTGGCCTAATTCGATCAAGATCAAGCCTCTACAGCCCTTGGTCAACCAATCTTTCCCAAATCGTCAACATAACAAGAAGTTCACACTACAACAGTTCGATTCAAGATCAAATGATCGCTAATCTTGGATCAAACCAACAACGGAGATTGAATCGAGGAAATCTTGTGAAAGAATCTAAAGCAACAAAATTCAAGATGTGTGTGAAAATGAGAACAAAGTCGGGCAAGGCATGCATCAAAAGAGTAGCTTGCATTGAGCCATTGGCCATCAAAACAATTACTGGACTGAGATCTGCCAGCTCTGACATCACAAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

28.56

Weight (kDa)

10.94

Isoelectric Point (pI)

64.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AciI CCGC 1 cut(s) 332
AclWI GGATC 1 cut(s) 604
AcoI YGGCCR 2 cut(s) 67, 732
AcsI RAATTY 1 cut(s) 656
AcvI CACGTG 2 cut(s) 229, 271
AcyI GRCGYC 1 cut(s) 160
AdeI CACNNNGTG 1 cut(s) 271
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 3 cut(s) 226, 228, 413
AgeI ACCGGT 1 cut(s) 277
AgsI TTSAA 5 cut(s) 291, 426, 572, 619, 661
AluBI AGCT 7 cut(s) 58, 176, 198, 253, 385, 716, 768
AluI AGCT 7 cut(s) 58, 176, 198, 253, 385, 716, 768
AlwI GGATC 1 cut(s) 604
Ama87I CYCGRG 2 cut(s) 45, 146
AoxI GGCC 4 cut(s) 60, 67, 472, 732
ApoI RAATTY 1 cut(s) 656
AsiGI ACCGGT 1 cut(s) 277
Asp700I GAANNNNTTC 1 cut(s) 361
AspLEI GCGC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 257, 444
AvaI CYCGRG 2 cut(s) 45, 146
BaeGI GKGCMC 1 cut(s) 219
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 46
BauI CACGAG 2 cut(s) 199, 386
BbrPI CACGTG 2 cut(s) 229, 271
BccI CCATC 4 cut(s) 103, 129, 136, 743
BceAI ACGGC 2 cut(s) 59, 330
BcgI CGANNNNNNTGC 4 cut(s) 213, 247, 295, 329
BclI TGATCA 1 cut(s) 325
BfmI CTRYAG 1 cut(s) 499
BglII AGATCT 1 cut(s) 758
BmeT110I CYCGRG 2 cut(s) 45, 146
BmiI GGNNCC 2 cut(s) 43, 218
BmsI GCATC 2 cut(s) 326, 711
BplI GAGNNNNNCTC 4 cut(s) 47, 79, 295, 327
BsaAI YACGTR 2 cut(s) 229, 271
BsaBI GATNNNNATC 1 cut(s) 487
BsaHI GRCGYC 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 506
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 754
Bse8I GATNNNNATC 1 cut(s) 487
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 3 cut(s) 26, 238, 317
BseJI GATNNNNATC 1 cut(s) 487
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 2 cut(s) 9, 746
BseNI ACTGG 1 cut(s) 754
BseSI GKGCMC 1 cut(s) 219
BshFI GGCC 4 cut(s) 62, 69, 474, 734
BshNI GGYRCC 1 cut(s) 216
BshTI ACCGGT 1 cut(s) 277
BsiHKCI CYCGRG 2 cut(s) 45, 146
BsiSI CCGG 3 cut(s) 66, 96, 278
BslI CCNNNNNNNGG 1 cut(s) 284
BsmI GAATGC 1 cut(s) 452
BsnI GGCC 4 cut(s) 62, 69, 474, 734
BsoBI CYCGRG 2 cut(s) 45, 146
Bsp1286I GDGCHC 2 cut(s) 46, 219
Bsp143I GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
BspACI CCGC 1 cut(s) 332
BspANI GGCC 4 cut(s) 62, 69, 474, 734
BspCNI CTCAG 2 cut(s) 10, 747
BspHI TCATGA 1 cut(s) 785
BspLI GGNNCC 2 cut(s) 43, 218
BspPI GGATC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
BssNI GRCGYC 1 cut(s) 160
BssSI CACGAG 2 cut(s) 199, 386
BssT1I CCWWGG 1 cut(s) 506
Bst2BI CACGAG 2 cut(s) 199, 386
Bst4CI ACNGT 1 cut(s) 563
BstACI GRCGYC 1 cut(s) 160
BstAPI GCANNNNNTGC 1 cut(s) 699
BstBAI YACGTR 2 cut(s) 229, 271
BstC8I GCNNGC 6 cut(s) 60, 196, 308, 700, 718, 766
BstDEI CTNAG 2 cut(s) 18, 755
BstF5I GGATG 3 cut(s) 26, 238, 317
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 9 cut(s) 17, 300, 328, 485, 491, 577, 585, 599, 761
BstMBI GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
BstMWI GCNNNNNNNGC 2 cut(s) 501, 699
BstNSI RCATGY 2 cut(s) 417, 702
BstSFI CTRYAG 1 cut(s) 499
BstSLI GKGCMC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 758
BstXI CCANNNNNNTGG 1 cut(s) 135
BstYI RGATCY 1 cut(s) 758
BsuRI GGCC 4 cut(s) 62, 69, 474, 734
BtsCI GGATG 3 cut(s) 26, 238, 317
Cac8I GCNNGC 6 cut(s) 60, 196, 308, 700, 718, 766
CciI TCATGA 1 cut(s) 785
CfoI GCGC 1 cut(s) 52
Cfr10I RCCGGY 1 cut(s) 277
CseI GACGC 1 cut(s) 149
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 7 cut(s) 53, 71, 82, 414, 458, 699, 786
DdeI CTNAG 2 cut(s) 18, 755
DpnI GATC 9 cut(s) 16, 299, 327, 484, 490, 576, 584, 598, 760
DpnII GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
DraIII CACNNNGTG 1 cut(s) 271
EaeI YGGCCR 2 cut(s) 67, 732
Eco130I CCWWGG 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 46
Eco72I CACGTG 2 cut(s) 229, 271
Eco88I CYCGRG 2 cut(s) 45, 146
EcoT14I CCWWGG 1 cut(s) 506
EcoT22I ATGCAT 1 cut(s) 704
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 506
FaeI CATG 7 cut(s) 56, 74, 85, 417, 461, 702, 789
FaiI YATR 8 cut(s) 54, 72, 83, 415, 459, 539, 700, 787
FalI AAGNNNNNCTT 6 cut(s) 160, 192, 346, 378, 700, 732
FatI CATG 7 cut(s) 52, 70, 81, 413, 457, 698, 785
FbaI TGATCA 1 cut(s) 325
FokI GGATG 3 cut(s) 13, 225, 304
FriOI GRGCYC 1 cut(s) 46
GlaI GCGC 1 cut(s) 51
HaeIII GGCC 4 cut(s) 62, 69, 474, 734
HapII CCGG 3 cut(s) 66, 96, 278
HgaI GACGC 1 cut(s) 149
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 7 cut(s) 56, 74, 85, 417, 461, 702, 789
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 4 cut(s) 164, 350, 513, 535
HindII GTYRAC 4 cut(s) 164, 350, 513, 535
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 6 cut(s) 207, 235, 394, 568, 619, 641
HpaII CCGG 3 cut(s) 66, 96, 278
HphI GGTGA 2 cut(s) 257, 444
Hpy166II GTNNAC 5 cut(s) 164, 350, 513, 535, 551
Hpy188I TCNGA 3 cut(s) 14, 19, 772
Hpy188III TCNNGA 6 cut(s) 132, 301, 486, 572, 661, 786
Hpy8I GTNNAC 5 cut(s) 164, 350, 513, 535, 551
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 345
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4IV ACGT 2 cut(s) 228, 270
HpyCH4V TGCA 2 cut(s) 702, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 501, 699
HpyF3I CTNAG 2 cut(s) 18, 755
HpySE526I ACGT 2 cut(s) 228, 270
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 7 cut(s) 56, 74, 85, 417, 461, 702, 789
HspAI GCGC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 6 cut(s) 44, 79, 109, 291, 735, 778
LweI GCATC 2 cut(s) 326, 711
MaeII ACGT 2 cut(s) 228, 270
MaeIII GTNAC 1 cut(s) 271
MalI GATC 9 cut(s) 16, 299, 327, 484, 490, 576, 584, 598, 760
MboI GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
MboII GAAGA 2 cut(s) 354, 367
MflI RGATCY 1 cut(s) 758
MhlI GDGCHC 2 cut(s) 46, 219
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 3 cut(s) 477, 656, 744
MluNI TGGCCA 1 cut(s) 734
MnlI CCTC 5 cut(s) 40, 73, 321, 506, 617
Mox20I TGGCCA 1 cut(s) 734
Mph1103I ATGCAT 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 361
MscI TGGCCA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 133
Msp20I TGGCCA 1 cut(s) 734
MspI CCGG 3 cut(s) 66, 96, 278
Mva1269I GAATGC 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 501, 699
NdeII GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
NlaIII CATG 7 cut(s) 56, 74, 85, 417, 461, 702, 789
NlaIV GGNNCC 2 cut(s) 43, 218
NmuCI GTSAC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 2 cut(s) 417, 702
PaeI GCATGC 1 cut(s) 702
PagI TCATGA 1 cut(s) 785
PciI ACATGT 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 6 cut(s) 207, 235, 394, 568, 619, 641
PinAI ACCGGT 1 cut(s) 277
PmaCI CACGTG 2 cut(s) 229, 271
PmlI CACGTG 2 cut(s) 229, 271
Ppu21I YACGTR 2 cut(s) 229, 271
PscI ACATGT 1 cut(s) 413
PspCI CACGTG 2 cut(s) 229, 271
PspN4I GGNNCC 2 cut(s) 43, 218
PsuI RGATCY 1 cut(s) 758
RseI CAYNNNNRTG 1 cut(s) 133
Sau3AI GATC 9 cut(s) 14, 297, 325, 482, 488, 574, 582, 596, 758
SduI GDGCHC 2 cut(s) 46, 219
SfaNI GCATC 2 cut(s) 326, 711
SfcI CTRYAG 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 133
SphI GCATGC 1 cut(s) 702
Sse9I AATT 3 cut(s) 477, 656, 744
SsiI CCGC 1 cut(s) 332
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 1 cut(s) 563
TaiI ACGT 2 cut(s) 231, 273
TaqI TCGA 5 cut(s) 131, 233, 481, 566, 622
TasI AATT 3 cut(s) 477, 656, 744
TfiI GAWTC 6 cut(s) 207, 235, 394, 568, 619, 641
TseFI GTSAC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 227, 367
TspGWI ACGGA 2 cut(s) 231, 625
XapI RAATTY 1 cut(s) 656
XceI RCATGY 2 cut(s) 417, 702
XmnI GAANNNNTTC 1 cut(s) 361
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.