Rroxscaffold_4G00319380

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003699
Physical Location & Seq
Reverse (-)
47161197 .. 47179298
18102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319380.1

Sequence Viewer

Length: 756 bp
ATGGATACCATATATATGAACAAAATATTGGATAGACACCCACAAATCATTCGGGAGGTGTATAGAATGTATCCTGATGTCTTTCAAAAATTATATAGCATCATAAAAGAGAAAACACCTTTACGAGATACAAGACATATTCTCGTGGAGAATCATCAAGCACCAAACACACGTGCCACTTCATCCCCTACCAAAGCCGAGGAACGCTCACCACGTGACACCACTCGTGGCCTAAATCTGATCAAGATCAAGCCTCTACAACCCTTGGTCAATCGTCTTCCTCAAATCGTCAACATAACAAGAAGTTCACACTACAACCGTTCGATTCAAGATCAAGTGCTCGCCACCCTTAGATCAAATCAACATCGGAGATCAAATCGAGAGGAGATTCTTGTGAAAGAGCATCTACGAGAGATTTCGAGTAAGACTACCACTTTCACAACATTAGGGGCAAACATTACTAAGCAAGGAAGTGTAGCATACTTAAGCAACTCAAGAGAGTATTCTCCTTCGCCTATTGCCGGTGTTGATCCGCCACTTTCAACATCCCAAAAAGGACCCACTTCCACAAAACAATCCTCAACAACTCAACGAGTTAAATCCGGAGGTTCCCATCCCTCGAAGATCATAACTCGCGGAAACGAACTTCTTCTAATACGAACCTACAAGACGATCTTTACGGAAGAGAAACTACGAGGACTGCCGGTGAGAGTTGGTGTTGACAGGCTGAAGAAGGATGAGTGCCTATCGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

251

Amino Acids

28.64

Weight (kDa)

10.25

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 4 - 51 5.9e-07 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 602
AciI CCGC 2 cut(s) 533, 636
AclWI GGATC 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 749
AcvI CACGTG 2 cut(s) 173, 215
AdeI CACNNNGTG 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 521
AflII CTTAAG 1 cut(s) 484
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 3 cut(s) 86, 329, 543
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 1 cut(s) 229
Asp700I GAANNNNTTC 1 cut(s) 648
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 2 cut(s) 201, 718
AvaII GGWCC 1 cut(s) 557
BarI GAAGNNNNNNTAC 2 cut(s) 675, 707
BauI CACGAG 2 cut(s) 143, 225
BbrPI CACGTG 2 cut(s) 173, 215
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 342
BccI CCATC 1 cut(s) 621
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 240
BfrI CTTAAG 1 cut(s) 484
BfuI GTATCC 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 559, 610
BmsI GCATC 2 cut(s) 108, 412
BpiI GAAGAC 1 cut(s) 269
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpuEI CTTGAG 1 cut(s) 478
Bsa29I ATCGAT 1 cut(s) 749
BsaAI YACGTR 2 cut(s) 173, 215
BsaBI GATNNNNATC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 198, 264
BsaWI WCCGGW 1 cut(s) 602
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 2 cut(s) 521, 703
Bse8I GATNNNNATC 1 cut(s) 245
BseAI TCCGGA 1 cut(s) 602
BseCI ATCGAT 1 cut(s) 749
BseDI CCNNGG 2 cut(s) 198, 264
BseGI GGATG 4 cut(s) 182, 545, 613, 742
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 521
BseRI GAGGAG 1 cut(s) 398
Bsh1236I CGCG 1 cut(s) 636
BshFI GGCC 1 cut(s) 231
BshVI ATCGAT 1 cut(s) 749
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 3 cut(s) 522, 603, 704
BslI CCNNNNNNNGG 1 cut(s) 521
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 342
Bsp13I TCCGGA 1 cut(s) 602
Bsp143I GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
BspACI CCGC 2 cut(s) 533, 636
BspANI GGCC 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 749
BspEI TCCGGA 1 cut(s) 602
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 559, 610
BspPI GGATC 1 cut(s) 524
BspTI CTTAAG 1 cut(s) 484
BsrFI RCCGGY 2 cut(s) 521, 703
BssAI RCCGGY 2 cut(s) 521, 703
BssECI CCNNGG 2 cut(s) 198, 264
BssMI GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
BssSI CACGAG 2 cut(s) 143, 225
BssT1I CCWWGG 1 cut(s) 264
Bst2BI CACGAG 2 cut(s) 143, 225
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 484
BstBAI YACGTR 2 cut(s) 173, 215
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 2 cut(s) 350, 462
BstF5I GGATG 4 cut(s) 182, 545, 613, 742
BstFNI CGCG 1 cut(s) 636
BstKTI GATC 8 cut(s) 243, 249, 334, 356, 374, 532, 627, 675
BstMBI GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
BstUI CGCG 1 cut(s) 636
BstV2I GAAGAC 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 749
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 1 cut(s) 231
BsuTUI ATCGAT 1 cut(s) 749
BtsCI GGATG 4 cut(s) 182, 545, 613, 742
Cac8I GCNNGC 1 cut(s) 342
Cfr10I RCCGGY 2 cut(s) 521, 703
Cfr13I GGNCC 1 cut(s) 557
ClaI ATCGAT 1 cut(s) 749
CviJI RGCY 4 cut(s) 197, 231, 253, 727
CviKI_1 RGCY 4 cut(s) 197, 231, 253, 727
DdeI CTNAG 2 cut(s) 350, 462
DpnI GATC 8 cut(s) 242, 248, 333, 355, 373, 531, 626, 674
DpnII GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
DraIII CACNNNGTG 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
EciI GGCGGA 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 749
Eco72I CACGTG 2 cut(s) 173, 215
EcoO109I RGGNCCY 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FbaI TGATCA 1 cut(s) 240
FokI GGATG 4 cut(s) 169, 532, 600, 749
HaeIII GGCC 1 cut(s) 231
HapII CCGG 3 cut(s) 522, 603, 704
HincII GTYRAC 2 cut(s) 292, 721
HindII GTYRAC 2 cut(s) 292, 721
HinfI GANTC 3 cut(s) 151, 325, 388
HpaII CCGG 3 cut(s) 522, 603, 704
HphI GGTGA 2 cut(s) 201, 718
Hpy166II GTNNAC 3 cut(s) 292, 308, 721
Hpy188I TCNGA 2 cut(s) 240, 369
Hpy188III TCNNGA 7 cut(s) 53, 74, 244, 329, 380, 495, 603
Hpy8I GTNNAC 3 cut(s) 292, 308, 721
HpyAV CCTTC 2 cut(s) 519, 727
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 2 cut(s) 172, 214
HpyF3I CTNAG 2 cut(s) 350, 462
HpySE526I ACGT 2 cut(s) 172, 214
Kpn2I TCCGGA 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
LpnPI CCDG 5 cut(s) 87, 535, 616, 709, 717
LweI GCATC 2 cut(s) 108, 412
MaeII ACGT 2 cut(s) 172, 214
MaeIII GTNAC 1 cut(s) 215
MalI GATC 8 cut(s) 242, 248, 333, 355, 373, 531, 626, 674
MboI GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
MboII GAAGA 5 cut(s) 269, 634, 641, 695, 742
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 1 cut(s) 89
MnlI CCTC 9 cut(s) 49, 193, 264, 291, 376, 589, 599, 628, 689
MroI TCCGGA 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 2 cut(s) 485, 597
MslI CAYNNNNRTG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 3 cut(s) 522, 603, 704
MvnI CGCG 1 cut(s) 636
NdeII GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
NlaIV GGNNCC 2 cut(s) 559, 610
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 151, 325, 388
PmaCI CACGTG 2 cut(s) 173, 215
PmlI CACGTG 2 cut(s) 173, 215
Ppu21I YACGTR 2 cut(s) 173, 215
PpuMI RGGWCCY 1 cut(s) 557
Psp5II RGGWCCY 1 cut(s) 557
PspCI CACGTG 2 cut(s) 173, 215
PspN4I GGNNCC 2 cut(s) 559, 610
PspPI GGNCC 1 cut(s) 557
PspPPI RGGWCCY 1 cut(s) 557
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 2 cut(s) 485, 597
Sau3AI GATC 8 cut(s) 240, 246, 331, 353, 371, 529, 624, 672
Sau96I GGNCC 1 cut(s) 557
SduI GDGCHC 1 cut(s) 342
SetI ASST 6 cut(s) 60, 121, 175, 217, 610, 665
SfaNI GCATC 2 cut(s) 108, 412
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 484, 493
SmoI CTYRAG 2 cut(s) 484, 493
Sse9I AATT 1 cut(s) 89
SsiI CCGC 2 cut(s) 533, 636
SspI AATATT 1 cut(s) 27
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 2 cut(s) 175, 217
TaqI TCGA 5 cut(s) 323, 379, 419, 620, 749
TasI AATT 1 cut(s) 89
TfiI GAWTC 3 cut(s) 151, 325, 388
Tru1I TTAA 2 cut(s) 485, 597
Tru9I TTAA 2 cut(s) 485, 597
TseFI GTSAC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 32, 171
TspGWI ACGGA 1 cut(s) 695
Vha464I CTTAAG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.