Rroxscaffold_3G00264010

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004554
Physical Location & Seq
Forward (+)
57798558 .. 57804200
5643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264010.1

Sequence Viewer

Length: 1197 bp
ATGACACTGAAGAGAGAACAGCTTTCAAAAGATGGTGCTGTAAGCAGGAAATTAGATAGAGCAGACCTGGAAGAGTGTAATTCAAACTGTACTTCTTCTGTGGATGTGAGGAAAGGAGGAAAGCTGGATTTGAGCTGCACATCTAATGTCGGAAAGGAAATAGTACCGTCGAACCTTTCTGATATACCAGAGTGGATACTTGTTGATATTCTGTCACTCCTCTCAGTCAAGGATGCGGCATTAATCAGTTTCACCTGCAAGGCATGGGAAAATTTGTGGACCTATTCTACAAATCTGACTTTTGTTAACCGCATATGCCCCTATTCAAACCTTGACATTGGACTGAAGATTAGAGAGAGGGAAAAGTATGTTGGTTGGGTCAGAGATGTTCTAAAGAAGTACAGAGGGACGAAGCTTGATAAGTTCCATATCTTCTTTGATTTGGACAGCAGCTATAAAAGCTACATTGATGACTGGGTAGAGTTTGCTCTCAAACACGATGTTAAAGCGTTGATCTTGGAAATGGTCAGCCCTTTTGATCCTTCTCATAATAACTATTCCTTTACTAAGCGGATTATGGGCGAGTCCCTCCAAGTATTGGTGCTTGAAAATGTTGATCTGGATGGAGAGGTTTTTCAGTATATATCTCATTTTCGAGGACTTGAACAACTATCAGTGACTGGTGCATCTGGTTTAAATCGCATCAGAATGTTTGGTTCTTCAGTACGTTTGAAGTCCTTAGTGGTCAGAAATTGTTGGAATCTTGAGATTATTGAGATCTGTGATTTACCTCATCTTGCTTTCTTTGAGTTTGAGGGAAGACCTATATGCCTGGACCTGAACTCAGTTCCAAATCTAGTGAAAGTGTCCCTTGGCGAGGATGACCTGGAATTCTTAAAAGTTGCCTTCAAACAGCTTGCTTGCTGTCTCTGTCAGCTAAGAACTCTTACGCTGGATAACACACTTAGTTTTCTGACACAAGAGAATGTTGTCTTTCCTATTCCTCAGTTACCAGAGCTTGTGTGCTTGGAGTTGCGAATCTCACCCGATGATGCATGTGTGCTGTTGCAATTGGCGTGTTTTTTGGATGCATCTCCTCGCTTGCAAAAGTTAGTTTTAAAGTTTGACTTTCTAGGGTCGGGGATAAATTTGTTGAAAGCCGAGAGCCATCCGCACAACAACTTAAAAGGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

398

Amino Acids

45.11

Weight (kDa)

5.63

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 195 - 374 1.2e-12 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 598
AciI CCGC 4 cut(s) 236, 310, 571, 1172
AclWI GGATC 1 cut(s) 533
AcsI RAATTY 3 cut(s) 271, 890, 1147
AcuI CTGAAG 3 cut(s) 29, 365, 705
AfaI GTAC 4 cut(s) 91, 165, 401, 726
AfiI CCNNNNNNNGG 2 cut(s) 598, 877
AgsI TTSAA 8 cut(s) 27, 84, 327, 608, 665, 733, 910, 1156
AjnI CCWGG 3 cut(s) 66, 831, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 9 cut(s) 22, 124, 135, 415, 453, 462, 916, 937, 1018
AluI AGCT 9 cut(s) 22, 124, 135, 415, 453, 462, 916, 937, 1018
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 680
ApeKI GCWGC 2 cut(s) 135, 450
ApoI RAATTY 3 cut(s) 271, 890, 1147
AseI ATTAAT 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 279, 835
AsuHPI GGTGA 2 cut(s) 244, 1035
AvaII GGWCC 2 cut(s) 279, 835
BbsI GAAGAC 1 cut(s) 826
BbvI GCAGC 2 cut(s) 122, 462
BccI CCATC 3 cut(s) 26, 617, 1176
BciT130I CCWGG 3 cut(s) 68, 833, 887
BciVI GTATCC 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 2 cut(s) 857, 1133
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 189
BglII AGATCT 1 cut(s) 777
BisI GCNGC 3 cut(s) 136, 237, 451
BlsI GCNGC 3 cut(s) 137, 238, 452
Bme1390I CCNGG 3 cut(s) 68, 833, 887
Bme18I GGWCC 2 cut(s) 279, 835
BmgT120I GGNCC 2 cut(s) 279, 835
BmrFI CCNGG 3 cut(s) 68, 833, 887
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 6 cut(s) 223, 695, 711, 1042, 1078, 1100
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 826
BpuEI CTTGAG 1 cut(s) 785
BsaJI CCNNGG 1 cut(s) 871
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 877
Bse1I ACTGG 2 cut(s) 479, 685
BseBI CCWGG 3 cut(s) 68, 833, 887
BseDI CCNNGG 1 cut(s) 871
BseGI GGATG 6 cut(s) 109, 238, 628, 886, 1093, 1168
BseLI CCNNNNNNNGG 2 cut(s) 598, 877
BseMII CTCAG 3 cut(s) 237, 858, 1019
BseNI ACTGG 2 cut(s) 479, 685
BseRI GAGGAG 2 cut(s) 209, 1086
BseXI GCAGC 2 cut(s) 122, 462
BsgI GTGCAG 1 cut(s) 121
BslFI GGGAC 3 cut(s) 421, 571, 853
BslI CCNNNNNNNGG 2 cut(s) 598, 877
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 3 cut(s) 421, 571, 853
Bsp143I GATC 4 cut(s) 513, 538, 616, 777
BspACI CCGC 4 cut(s) 236, 310, 571, 1172
BspCNI CTCAG 3 cut(s) 236, 857, 1018
BspMI ACCTGC 1 cut(s) 263
BspPI GGATC 1 cut(s) 533
BsrI ACTGG 2 cut(s) 479, 685
BssECI CCNNGG 1 cut(s) 871
BssMI GATC 4 cut(s) 513, 538, 616, 777
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 3 cut(s) 68, 833, 887
Bst4CI ACNGT 2 cut(s) 89, 168
Bst6I CTCTTC 2 cut(s) 5, 66
BstC8I GCNNGC 3 cut(s) 918, 922, 1103
BstDEI CTNAG 7 cut(s) 223, 567, 739, 844, 938, 965, 1005
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 6 cut(s) 109, 238, 628, 886, 1093, 1168
BstKTI GATC 4 cut(s) 516, 541, 619, 780
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 4 cut(s) 513, 538, 616, 777
BstMWI GCNNNNNNNGC 1 cut(s) 459
BstNI CCWGG 3 cut(s) 68, 833, 887
BstNSI RCATGY 1 cut(s) 1059
BstSCI CCNGG 3 cut(s) 66, 831, 885
BstV1I GCAGC 2 cut(s) 122, 462
BstV2I GAAGAC 1 cut(s) 826
BstX2I RGATCY 1 cut(s) 777
BstYI RGATCY 1 cut(s) 777
BsuI GTATCC 1 cut(s) 189
BtsCI GGATG 6 cut(s) 109, 238, 628, 886, 1093, 1168
BtsIMutI CAGTG 2 cut(s) 5, 681
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 3 cut(s) 918, 922, 1103
CaiI CAGNNNCTG 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 279, 835
Csp6I GTAC 4 cut(s) 90, 164, 400, 725
CviAII CATG 2 cut(s) 264, 1056
CviQI GTAC 4 cut(s) 90, 164, 400, 725
DdeI CTNAG 7 cut(s) 223, 567, 739, 844, 938, 965, 1005
DpnI GATC 4 cut(s) 515, 540, 618, 779
DpnII GATC 4 cut(s) 513, 538, 616, 777
DraI TTTAAA 2 cut(s) 696, 1119
Eam1104I CTCTTC 2 cut(s) 5, 66
EarI CTCTTC 2 cut(s) 5, 66
Eco130I CCWWGG 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 279, 835
Eco57I CTGAAG 3 cut(s) 29, 365, 705
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 890
EcoRII CCWGG 3 cut(s) 66, 831, 885
EcoT14I CCWWGG 1 cut(s) 871
EcoT22I ATGCAT 2 cut(s) 1057, 1093
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 2 cut(s) 267, 1059
FalI AAGNNNNNCTT 4 cut(s) 855, 887, 1112, 1144
FaqI GGGAC 3 cut(s) 421, 571, 853
FatI CATG 2 cut(s) 263, 1055
FauNDI CATATG 1 cut(s) 314
Fnu4HI GCNGC 3 cut(s) 136, 237, 451
FokI GGATG 6 cut(s) 116, 245, 635, 893, 1100, 1155
Fsp4HI GCNGC 3 cut(s) 136, 237, 451
FspBI CTAG 2 cut(s) 857, 1133
GluI GCNGC 3 cut(s) 136, 237, 451
Hin1II CATG 2 cut(s) 267, 1059
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 3 cut(s) 584, 760, 1038
HpaI GTTAAC 1 cut(s) 307
HphI GGTGA 2 cut(s) 244, 1035
Hpy166II GTNNAC 2 cut(s) 279, 307
Hpy188I TCNGA 7 cut(s) 152, 181, 297, 383, 707, 749, 975
Hpy188III TCNNGA 2 cut(s) 620, 764
Hpy8I GTNNAC 2 cut(s) 279, 307
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 2 cut(s) 552, 916
HpyCH4III ACNGT 2 cut(s) 89, 168
HpyCH4IV ACGT 1 cut(s) 727
HpyCH4V TGCA 7 cut(s) 138, 258, 686, 1055, 1069, 1091, 1105
HpyF10VI GCNNNNNNNGC 1 cut(s) 459
HpyF3I CTNAG 7 cut(s) 223, 567, 739, 844, 938, 965, 1005
HpySE526I ACGT 1 cut(s) 727
Hsp92II CATG 2 cut(s) 267, 1059
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 4 cut(s) 513, 538, 616, 777
Lsp1109I GCAGC 2 cut(s) 122, 462
LweI GCATC 6 cut(s) 223, 695, 711, 1042, 1078, 1100
MaeI CTAG 2 cut(s) 857, 1133
MaeII ACGT 1 cut(s) 727
MaeIII GTNAC 3 cut(s) 213, 676, 1008
MalI GATC 4 cut(s) 515, 540, 618, 779
MboI GATC 4 cut(s) 513, 538, 616, 777
MboII GAAGA 7 cut(s) 22, 83, 87, 358, 424, 711, 831
MfeI CAATTG 1 cut(s) 1070
MflI RGATCY 1 cut(s) 777
MluCI AATT 7 cut(s) 50, 79, 271, 751, 890, 1070, 1147
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 2 cut(s) 130, 737
Mph1103I ATGCAT 2 cut(s) 1057, 1093
MseI TTAA 7 cut(s) 242, 306, 504, 695, 896, 1118, 1184
MslI CAYNNNNRTG 1 cut(s) 707
MspR9I CCNGG 3 cut(s) 68, 833, 887
MunI CAATTG 1 cut(s) 1070
MvaI CCWGG 3 cut(s) 68, 833, 887
MwoI GCNNNNNNNGC 1 cut(s) 459
NdeI CATATG 1 cut(s) 314
NdeII GATC 4 cut(s) 513, 538, 616, 777
NlaIII CATG 2 cut(s) 267, 1059
NmeAIII GCCGAG 1 cut(s) 1186
NmuCI GTSAC 2 cut(s) 213, 676
NsiI ATGCAT 2 cut(s) 1057, 1093
NspI RCATGY 1 cut(s) 1059
PaqCI CACCTGC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 760, 1038
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 3 cut(s) 137, 238, 452
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 242
Psp6I CCWGG 3 cut(s) 66, 831, 885
PspGI CCWGG 3 cut(s) 66, 831, 885
PspPI GGNCC 2 cut(s) 279, 835
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 777
RsaI GTAC 4 cut(s) 91, 165, 401, 726
RsaNI GTAC 4 cut(s) 90, 164, 400, 725
RseI CAYNNNNRTG 1 cut(s) 707
SaqAI TTAA 7 cut(s) 242, 306, 504, 695, 896, 1118, 1184
SatI GCNGC 3 cut(s) 136, 237, 451
Sau3AI GATC 4 cut(s) 513, 538, 616, 777
Sau96I GGNCC 2 cut(s) 279, 835
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 3 cut(s) 68, 833, 887
SfaNI GCATC 6 cut(s) 223, 695, 711, 1042, 1078, 1100
SinI GGWCC 2 cut(s) 279, 835
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 7 cut(s) 50, 79, 271, 751, 890, 1070, 1147
SsiI CCGC 4 cut(s) 236, 310, 571, 1172
SspMI CTAG 2 cut(s) 857, 1133
StyD4I CCNGG 3 cut(s) 66, 831, 885
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 2 cut(s) 89, 168
TaiI ACGT 1 cut(s) 730
TaqI TCGA 2 cut(s) 170, 655
TasI AATT 7 cut(s) 50, 79, 271, 751, 890, 1070, 1147
TatI WGTACW 2 cut(s) 89, 399
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 2 cut(s) 760, 1038
Tru1I TTAA 7 cut(s) 242, 306, 504, 695, 896, 1118, 1184
Tru9I TTAA 7 cut(s) 242, 306, 504, 695, 896, 1118, 1184
TscAI CASTG 2 cut(s) 12, 681
TseFI GTSAC 2 cut(s) 213, 676
TseI GCWGC 2 cut(s) 135, 450
Tsp45I GTSAC 2 cut(s) 213, 676
TspRI CASTG 2 cut(s) 12, 681
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 279, 835
VspI ATTAAT 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 3 cut(s) 271, 890, 1147
XceI RCATGY 1 cut(s) 1059
XspI CTAG 2 cut(s) 857, 1133
Zsp2I ATGCAT 2 cut(s) 1057, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.