Rorug01G0306800

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002727
Physical Location & Seq
Reverse (-)
42376080 .. 42379387
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0306800.1

Sequence Viewer

Length: 1191 bp
ATGGCGAAGAAAAAAGACCGCAGAGTCGTCGCGCCGAGTTCCGATCGCCGCAAAGTCCGAGTCGAACTGTCGCACCAACTCTCCCACTCAGCCTCATCGTCCTCAAATCGGAAGCTCGTAATCACCTTCGTCCTCTTCTTCGTCGTATCTTCAGCAATCTCGGAAAATGCAGTTGTTTCGGAAAATACGACTGGCAGGCACTATACTTATCCTGTATTAGCTTACATTACTCCATGGAATTCTAAGGGCTATGACTTGGCAAAAAGGTTTAACTCCAAATTTACACATATATCTCCAGTCTGGTATGATCTGAAGAGTCAAGGGACCAGCTTAATTCTGGAAGGCAGACATAATGCTGATATTGGGTGGATCTCAGAACTTAGAATAGCAGGAGACGCTTGGGTATTGCCTAGAATTGTTCTGGAAGCTTTTCCAGCAGAGTTGCTTACAAAGAAAAAGCAGAGGAATAAGGCTATTGATCTTATAGTAAGCGAGTGCAAGGAAATGGGCTATGATGGAATTGTGCTGGAATCTTGGTCAAGGTGGGCAGCTTACCGAGTTTTGCATGATCCAAAGATGCGGAATTTGGCATTACAGTTTATAAAAGATCTTGGAGATGCACTGCATGCTGTGACTTCAGAGAGGAACAATAAGCACCGCTTGCAGTTGGTGTATGTTATAGGCCCACCACATTCAGAGAAGCTCCAAGTGCATGATTTTGGACCTAAAGATCTTCAGAGCTTGAGTGATGCTGTGGATGGATTCTCACTTATGACGTATGACTTCTCAGGGCCTCATAATCCAGGTCCCAATGCACCTCTGAAGTGGATCGATTCCACCCTCCAGCTGCTCCTTGGTACCAATAAAAACAGTGCTCTGGCCAGCAAGATACTTCTTGGCATCAATTTCTATGGAAATGATTACATCCTCTCAGGAGGTCCTGGTGGTGGTGCTATCACCGGAAGAGATTACCTCTCCTTGTTGGAGAAGCACAAGCCTGCATTTCGATGGGAAAAGAACAGCGCAGAACATTTGTTCTTGTACACTGATAATGATCATAACAATCACGCTGTGTTCTACCCATCGTTACTGTCAATTTCCATGCGACTAGAGGAAGCTCGTAAATGGGGTTGTGGCATCTCGATATGGGAAATTGGGCAAGGTCTGGATTACTTTTACGATCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
V-ATPase_G

Protein Analysis

396

Amino Acids

44.67

Weight (kDa)

9.02

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 148 - 387 1.8e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AasI GACNNNNNNGTC 1 cut(s) 23
Acc65I GGTACC 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 857
AccII CGCG 1 cut(s) 32
AciI CCGC 4 cut(s) 19, 49, 580, 658
AclWI GGATC 3 cut(s) 377, 563, 836
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 3 cut(s) 238, 278, 583
AcuI CTGAAG 5 cut(s) 135, 332, 621, 719, 842
AdeI CACNNNGTG 1 cut(s) 1072
AfaI GTAC 2 cut(s) 859, 1043
AfiI CCNNNNNNNGG 2 cut(s) 108, 947
AjnI CCWGG 2 cut(s) 802, 940
AluBI AGCT 9 cut(s) 115, 221, 330, 428, 551, 703, 741, 847, 1118
AluI AGCT 9 cut(s) 115, 221, 330, 428, 551, 703, 741, 847, 1118
Alw21I GWGCWC 1 cut(s) 877
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 3 cut(s) 377, 563, 836
AoxI GGCC 3 cut(s) 682, 791, 879
ApeKI GCWGC 2 cut(s) 548, 847
ApoI RAATTY 3 cut(s) 238, 278, 583
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
Asp700I GAANNNNTTC 1 cut(s) 429
Asp718I GGTACC 1 cut(s) 857
AspLEI GCGC 2 cut(s) 34, 1025
AspS9I GGNCC 6 cut(s) 324, 683, 722, 791, 806, 938
AsuHPI GGTGA 2 cut(s) 115, 949
AvaII GGWCC 4 cut(s) 324, 722, 806, 938
BalI TGGCCA 1 cut(s) 881
BanI GGYRCC 1 cut(s) 857
Bbv12I GWGCWC 1 cut(s) 877
BbvI GCAGC 2 cut(s) 560, 834
BccI CCATC 4 cut(s) 509, 752, 1002, 1090
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 159, 193
BciT130I CCWGG 2 cut(s) 804, 942
BclI TGATCA 1 cut(s) 1054
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 2 cut(s) 411, 1109
BglII AGATCT 2 cut(s) 607, 730
BisI GCNGC 3 cut(s) 49, 549, 848
BlsI GCNGC 3 cut(s) 50, 550, 849
Bme1390I CCNGG 2 cut(s) 804, 942
Bme18I GGWCC 4 cut(s) 324, 722, 806, 938
BmgT120I GGNCC 6 cut(s) 324, 683, 722, 791, 806, 938
BmiI GGNNCC 3 cut(s) 325, 808, 859
BmrFI CCNGG 2 cut(s) 804, 942
BmsI GCATC 5 cut(s) 567, 607, 739, 909, 1146
BplI GAGNNNNNCTC 2 cut(s) 957, 989
BpmI CTGGAG 2 cut(s) 279, 827
BpuEI CTTGAG 1 cut(s) 763
Bsa29I ATCGAT 1 cut(s) 831
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 2 cut(s) 233, 853
BsaWI WCCGGW 1 cut(s) 959
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 947
Bse1I ACTGG 2 cut(s) 196, 296
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 2 cut(s) 804, 942
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 2 cut(s) 233, 853
BseGI GGATG 2 cut(s) 763, 924
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 2 cut(s) 108, 947
BseMII CTCAG 4 cut(s) 102, 387, 801, 945
BseNI ACTGG 2 cut(s) 196, 296
BseXI GCAGC 2 cut(s) 560, 834
Bsh1236I CGCG 1 cut(s) 32
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 3 cut(s) 684, 793, 881
BshNI GGYRCC 1 cut(s) 857
BshVI ATCGAT 1 cut(s) 831
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 877
BsiSI CCGG 1 cut(s) 960
BslFI GGGAC 2 cut(s) 337, 792
BslI CCNNNNNNNGG 2 cut(s) 108, 947
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 2 cut(s) 337, 792
BsnI GGCC 3 cut(s) 684, 793, 881
Bsp1286I GDGCHC 1 cut(s) 877
Bsp1407I TGTACA 1 cut(s) 1041
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 4 cut(s) 19, 49, 580, 658
BspANI GGCC 3 cut(s) 684, 793, 881
BspCNI CTCAG 4 cut(s) 101, 386, 800, 944
BspDI ATCGAT 1 cut(s) 831
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 3 cut(s) 325, 808, 859
BspPI GGATC 3 cut(s) 377, 563, 836
BspT107I GGYRCC 1 cut(s) 857
BsrGI TGTACA 1 cut(s) 1041
BsrI ACTGG 2 cut(s) 196, 296
BssECI CCNNGG 2 cut(s) 233, 853
BssT1I CCWWGG 2 cut(s) 233, 853
Bst2UI CCWGG 2 cut(s) 804, 942
Bst4CI ACNGT 4 cut(s) 69, 597, 872, 1092
Bst6I CTCTTC 3 cut(s) 140, 308, 958
BstAPI GCANNNNNTGC 2 cut(s) 626, 661
BstAUI TGTACA 1 cut(s) 1041
BstC8I GCNNGC 5 cut(s) 197, 627, 662, 883, 999
BstDEI CTNAG 6 cut(s) 88, 243, 373, 380, 787, 931
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 763, 924
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 2 cut(s) 34, 1025
BstMAI GTCTC 1 cut(s) 387
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 5 cut(s) 395, 434, 626, 661, 709
BstNI CCWGG 2 cut(s) 804, 942
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 802, 940
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 560, 834
BstX2I RGATCY 3 cut(s) 369, 607, 730
BstYI RGATCY 3 cut(s) 369, 607, 730
Bsu15I ATCGAT 1 cut(s) 831
BsuRI GGCC 3 cut(s) 684, 793, 881
BsuTUI ATCGAT 1 cut(s) 831
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 2 cut(s) 763, 924
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 3 cut(s) 620, 877, 1044
Cac8I GCNNGC 5 cut(s) 197, 627, 662, 883, 999
CfoI GCGC 2 cut(s) 34, 1025
Cfr13I GGNCC 6 cut(s) 324, 683, 722, 791, 806, 938
ClaI ATCGAT 1 cut(s) 831
CseI GACGC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 858, 1042
CviAII CATG 5 cut(s) 234, 566, 626, 713, 1102
CviQI GTAC 2 cut(s) 858, 1042
DdeI CTNAG 6 cut(s) 88, 243, 373, 380, 787, 931
DraIII CACNNNGTG 1 cut(s) 1072
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 3 cut(s) 140, 308, 958
EarI CTCTTC 3 cut(s) 140, 308, 958
Eco130I CCWWGG 2 cut(s) 233, 853
Eco47I GGWCC 4 cut(s) 324, 722, 806, 938
Eco57I CTGAAG 5 cut(s) 135, 332, 621, 719, 842
EcoO109I RGGNCCY 3 cut(s) 791, 806, 938
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 802, 940
EcoT14I CCWWGG 2 cut(s) 233, 853
ErhI CCWWGG 2 cut(s) 233, 853
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 5 cut(s) 237, 569, 629, 716, 1105
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 2 cut(s) 337, 792
FatI CATG 5 cut(s) 233, 565, 625, 712, 1101
FbaI TGATCA 1 cut(s) 1054
Fnu4HI GCNGC 3 cut(s) 49, 549, 848
FokI GGATG 2 cut(s) 770, 911
Fsp4HI GCNGC 3 cut(s) 49, 549, 848
FspBI CTAG 2 cut(s) 411, 1109
GlaI GCGC 2 cut(s) 33, 1024
GluI GCNGC 3 cut(s) 49, 549, 848
GsuI CTGGAG 2 cut(s) 279, 827
HaeIII GGCC 3 cut(s) 684, 793, 881
HapII CCGG 1 cut(s) 960
HgaI GACGC 1 cut(s) 404
HhaI GCGC 2 cut(s) 34, 1025
Hin1II CATG 5 cut(s) 237, 569, 629, 716, 1105
Hin6I GCGC 2 cut(s) 32, 1023
HinP1I GCGC 2 cut(s) 32, 1023
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 6 cut(s) 24, 60, 316, 530, 762, 833
HpaII CCGG 1 cut(s) 960
HphI GGTGA 2 cut(s) 115, 949
Hpy166II GTNNAC 1 cut(s) 1044
Hpy188III TCNNGA 5 cut(s) 338, 422, 933, 1141, 1166
Hpy8I GTNNAC 1 cut(s) 1044
Hpy99I CGWCG 2 cut(s) 32, 146
HpyAV CCTTC 2 cut(s) 136, 335
HpyCH4III ACNGT 4 cut(s) 69, 597, 872, 1092
HpyCH4IV ACGT 1 cut(s) 776
HpyCH4V TGCA 9 cut(s) 170, 498, 565, 620, 625, 664, 712, 815, 1001
HpyF10VI GCNNNNNNNGC 5 cut(s) 395, 434, 626, 661, 709
HpyF3I CTNAG 6 cut(s) 88, 243, 373, 380, 787, 931
HpySE526I ACGT 1 cut(s) 776
Hsp92II CATG 5 cut(s) 237, 569, 629, 716, 1105
HspAI GCGC 2 cut(s) 32, 1023
KpnI GGTACC 1 cut(s) 861
Ksp22I TGATCA 1 cut(s) 1054
LmnI GCTCC 2 cut(s) 708, 855
Lsp1109I GCAGC 2 cut(s) 560, 834
LweI GCATC 5 cut(s) 567, 607, 739, 909, 1146
MaeI CTAG 2 cut(s) 411, 1109
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 2 cut(s) 631, 1086
MboII GAAGA 8 cut(s) 19, 127, 130, 141, 325, 725, 975, 1175
MflI RGATCY 3 cut(s) 369, 607, 730
MhlI GDGCHC 1 cut(s) 877
MlsI TGGCCA 1 cut(s) 881
MluCI AATT 9 cut(s) 238, 278, 333, 414, 519, 583, 904, 1095, 1152
MluNI TGGCCA 1 cut(s) 881
MlyI GAGTC 3 cut(s) 33, 69, 325
MmeI TCCRAC 1 cut(s) 963
Mox20I TGGCCA 1 cut(s) 881
MroXI GAANNNNTTC 1 cut(s) 429
MscI TGGCCA 1 cut(s) 881
MseI TTAA 2 cut(s) 270, 332
MslI CAYNNNNRTG 1 cut(s) 1006
Msp20I TGGCCA 1 cut(s) 881
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 1 cut(s) 960
MspR9I CCNGG 2 cut(s) 804, 942
MvaI CCWGG 2 cut(s) 804, 942
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 5 cut(s) 395, 434, 626, 661, 709
NcoI CCATGG 1 cut(s) 233
NlaIII CATG 5 cut(s) 237, 569, 629, 716, 1105
NlaIV GGNNCC 3 cut(s) 325, 808, 859
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 1 cut(s) 629
PaeI GCATGC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 530, 762, 833
PkrI GCNGC 3 cut(s) 50, 550, 849
Ple19I CGATCG 1 cut(s) 46
PleI GAGTC 3 cut(s) 32, 68, 324
PpsI GAGTC 3 cut(s) 32, 68, 324
PpuMI RGGWCCY 2 cut(s) 806, 938
PsiI TTATAA 1 cut(s) 602
Psp5II RGGWCCY 2 cut(s) 806, 938
Psp6I CCWGG 2 cut(s) 802, 940
PspGI CCWGG 2 cut(s) 802, 940
PspN4I GGNNCC 3 cut(s) 325, 808, 859
PspPI GGNCC 6 cut(s) 324, 683, 722, 791, 806, 938
PspPPI RGGWCCY 2 cut(s) 806, 938
PsuI RGATCY 3 cut(s) 369, 607, 730
PvuI CGATCG 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 2 cut(s) 859, 1043
RsaNI GTAC 2 cut(s) 858, 1042
RseI CAYNNNNRTG 1 cut(s) 1006
SaqAI TTAA 2 cut(s) 270, 332
SatI GCNGC 3 cut(s) 49, 549, 848
Sau96I GGNCC 6 cut(s) 324, 683, 722, 791, 806, 938
SchI GAGTC 3 cut(s) 33, 69, 325
ScrFI CCNGG 2 cut(s) 804, 942
SduI GDGCHC 1 cut(s) 877
SfaNI GCATC 5 cut(s) 567, 607, 739, 909, 1146
SinI GGWCC 4 cut(s) 324, 722, 806, 938
SmiMI CAYNNNNRTG 1 cut(s) 1006
SmlI CTYRAG 1 cut(s) 742
SmoI CTYRAG 1 cut(s) 742
SphI GCATGC 1 cut(s) 629
Sse9I AATT 9 cut(s) 238, 278, 333, 414, 519, 583, 904, 1095, 1152
SsiI CCGC 4 cut(s) 19, 49, 580, 658
SspMI CTAG 2 cut(s) 411, 1109
StyD4I CCNGG 2 cut(s) 802, 940
StyI CCWWGG 2 cut(s) 233, 853
TaaI ACNGT 4 cut(s) 69, 597, 872, 1092
TaiI ACGT 1 cut(s) 779
TaqI TCGA 4 cut(s) 63, 831, 1006, 1142
TasI AATT 9 cut(s) 238, 278, 333, 414, 519, 583, 904, 1095, 1152
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 3 cut(s) 530, 762, 833
Tru1I TTAA 2 cut(s) 270, 332
Tru9I TTAA 2 cut(s) 270, 332
TscAI CASTG 3 cut(s) 627, 877, 1051
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 2 cut(s) 548, 847
Tsp45I GTSAC 1 cut(s) 631
TspRI CASTG 3 cut(s) 627, 877, 1051
VpaK11BI GGWCC 4 cut(s) 324, 722, 806, 938
XapI RAATTY 3 cut(s) 238, 278, 583
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 2 cut(s) 334, 851
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 2 cut(s) 411, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.