Rh1DG310400

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002727
Physical Location & Seq
Forward (+)
53727160 .. 53727924
765 bp
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UTR
Exon/CDS
Intron
Rh1DG310400.1

Sequence Viewer

Length: 330 bp
ATGGAAGTTAACAACGAACATAACACAATTCAACAGCAACTAACTGTGGAGCAAGAAGTTCAGCAAATTTTGAATGCTTCCAGAAATGCTAAAATGGCTAAACTAATACAGGCTAAAGAGGAAGCTGAGAAGGAGAATTCTGATTTTCGTGCTCAAACGGAAGTTGACTTTCAGAGAAAACTTGCAGAGGGAAGTGGACATTCAGGTCCTAATCTGACGAGGATTGAGCAAGAGACAGAGGCAAAGATCCATCATTTGAAGATTGATGCTGCAAGAATATCAAATGACATTGTGCGGATGCTTTTGGAATATACAACTGTGACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
V-ATPase_G

Protein Analysis

109

Amino Acids

12.46

Weight (kDa)

5.35

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 106 4.4e-20 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 204
AciI CCGC 1 cut(s) 295
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 2 cut(s) 66, 136
AgsI TTSAA 3 cut(s) 32, 73, 259
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 2 cut(s) 66, 136
AspS9I GGNCC 1 cut(s) 206
AvaII GGWCC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 256
BccI CCATC 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 227
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmsI GCATC 2 cut(s) 256, 288
BseGI GGATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 117
BseXI GCAGC 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 154
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 1 cut(s) 295
BspCNI CTCAG 1 cut(s) 118
BspPI GGATC 1 cut(s) 241
BssMI GATC 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 46, 319
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 303
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 256
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BtsCI GGATG 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 206
CviJI RGCY 3 cut(s) 98, 113, 125
CviKI_1 RGCY 3 cut(s) 98, 113, 125
DdeI CTNAG 1 cut(s) 126
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DrdI GACNNNNNNGTC 1 cut(s) 204
DseDI GACNNNNNNGTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 136
FaiI YATR 2 cut(s) 21, 312
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 270
HincII GTYRAC 2 cut(s) 10, 166
HindII GTYRAC 2 cut(s) 10, 166
HpaI GTTAAC 1 cut(s) 10
Hpy166II GTNNAC 3 cut(s) 10, 166, 197
Hpy188I TCNGA 3 cut(s) 142, 174, 216
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 3 cut(s) 10, 166, 197
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 2 cut(s) 46, 319
HpyCH4V TGCA 2 cut(s) 185, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 1 cut(s) 126
KspAI GTTAAC 1 cut(s) 10
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 3 cut(s) 94, 95, 189
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 2 cut(s) 256, 288
MaeIII GTNAC 1 cut(s) 319
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 271
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 3 cut(s) 27, 66, 136
MnlI CCTC 4 cut(s) 112, 181, 213, 232
MseI TTAA 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 95
NdeII GATC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 319
PctI GAATGC 1 cut(s) 79
PkrI GCNGC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
PspPI GGNCC 1 cut(s) 206
PspPPI RGGWCCY 1 cut(s) 206
PsuI RGATCY 1 cut(s) 246
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 154
SetI ASST 2 cut(s) 127, 208
SfaNI GCATC 2 cut(s) 256, 288
SgeI CNNG 9 cut(s) 65, 93, 122, 161, 194, 216, 231, 242, 285
SinI GGWCC 1 cut(s) 206
Sse9I AATT 3 cut(s) 27, 66, 136
SsiI CCGC 1 cut(s) 295
TaaI ACNGT 2 cut(s) 46, 319
TasI AATT 3 cut(s) 27, 66, 136
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 319
TspGWI ACGGA 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 2 cut(s) 66, 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.