Rorug01G0387700

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011596
Physical Location & Seq
Reverse (-)
49894132 .. 49896254
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0387700.1

Sequence Viewer

Length: 873 bp
ATGCAGGCCAAGGAGGAAGATGTGAGTGTGGGAGCCAACAAGTTCCCGGAGAGGCAGCCGATCGGTATCGCCGCTCAAACCCATGACGAGGGCAAGGACTACAAGGAGCCACCGCCTGCGCCGCTCTTCGAGCCCGGCGAGCTGACGTCATGGTCGTTTTACAGGGCCGGGATCGCCGAGTTCGTCGCCACGTTTCTGTTTCTCTACATCACCATCTTGACGGTGATGGGCGTCTTGAAAGGTGAAACCAAGTGCAAAACCGTAGGTATTCAAGGCATCGCTTGGGCTTTTGGTGGTATGATCTTCGCCTTGGTTTACTGCACCGCCGGAATCTCAGGGGGTCACATAAACCCGGCTGTGACTTTTGGGCTTTTCTTGGCCCGGAAGCTGTCCTTGACAAGGGCGGTGTTCTACATCATCATGCAGTGCCTTGGGGCTATAGCCGGAGCCGCCGTGGTGAAAGCTTTTACGAACAAGGGTCATTTCTTCGAGATCAACAACGGTGGCGCCAACTTTGTAGCCCATGGCTACACCAAGGGCAGTGGTCTTGGTGCTGAAATTATTGGCACCTTTGTCCTTGTCTACACCGTCTTCTCTGCCACTGACGCCAAGCGTAGCGCCAGAGACTCTCATGTTCCGATTTTGGCCCCGTTGCCAATTGGGTTCGCCGTGTTCTTGGTGCACTTGGCTACCATCCCCATTACCGGAACCGGTATTAACCCAGCTAGGAGTCTTGGTGCAGCCATCATCTTCAACAAGGACAGAGCCTGGGATGACCAATGGATTTTCTGGGTTGGACCATTCATTGGGGCAGCACTTGCAGCTTTATACCATGTGGTTGTGATCAGAGCCATTCCTTTCAAATCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

290

Amino Acids

31.1

Weight (kDa)

9.06

Isoelectric Point (pI)

25.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 47 - 278 3e-82 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 151
AatII GACGTC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 506, 566
AccB7I CCANNNNNTGG 1 cut(s) 806
AccBSI CCGCTC 2 cut(s) 74, 124
AccI GTMKAC 1 cut(s) 582
AciI CCGC 6 cut(s) 72, 113, 122, 324, 404, 450
AclWI GGATC 1 cut(s) 179
AcyI GRCGYC 4 cut(s) 146, 231, 507, 606
AfiI CCNNNNNNNGG 4 cut(s) 88, 399, 704, 806
AgeI ACCGGT 1 cut(s) 710
AgsI TTSAA 4 cut(s) 238, 272, 754, 862
AjnI CCWGG 1 cut(s) 767
AluBI AGCT 5 cut(s) 142, 388, 464, 725, 824
AluI AGCT 5 cut(s) 142, 388, 464, 725, 824
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 1 cut(s) 618
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 768
AoxI GGCC 4 cut(s) 6, 165, 378, 645
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 4 cut(s) 55, 740, 812, 821
AsiGI ACCGGT 1 cut(s) 710
AspLEI GCGC 3 cut(s) 121, 509, 620
AspS9I GGNCC 4 cut(s) 165, 379, 646, 797
AsuC2I CCSGG 5 cut(s) 47, 135, 169, 353, 382
AsuHPI GGTGA 4 cut(s) 202, 235, 254, 469
AvaII GGWCC 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 684
BanI GGYRCC 2 cut(s) 506, 566
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 67, 752, 824, 833
BccI CCATC 4 cut(s) 220, 221, 701, 752
BceAI ACGGC 2 cut(s) 437, 653
BciT130I CCWGG 1 cut(s) 769
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 5 cut(s) 47, 135, 169, 353, 382
BcoDI GTCTC 1 cut(s) 618
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 2 cut(s) 510, 621
BisI GCNGC 7 cut(s) 56, 72, 122, 450, 741, 813, 822
BlsI GCNGC 7 cut(s) 57, 73, 123, 451, 742, 814, 823
Bme1390I CCNGG 6 cut(s) 47, 135, 169, 353, 382, 769
Bme18I GGWCC 1 cut(s) 797
BmgT120I GGNCC 4 cut(s) 165, 379, 646, 797
BmiI GGNNCC 7 cut(s) 34, 108, 448, 508, 568, 648, 709
BmrFI CCNGG 6 cut(s) 47, 135, 169, 353, 382, 769
BmsI GCATC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 583
BpuMI CCSGG 5 cut(s) 47, 135, 169, 353, 382
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 4 cut(s) 146, 231, 507, 606
BsaJI CCNNGG 7 cut(s) 9, 309, 430, 453, 523, 534, 768
BsaWI WCCGGW 2 cut(s) 704, 710
BsaXI ACNNNNNCTCC 4 cut(s) 438, 468, 721, 751
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 399, 704, 806
Bse118I RCCGGY 1 cut(s) 710
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 1 cut(s) 769
BseDI CCNNGG 7 cut(s) 9, 309, 430, 453, 523, 534, 768
BseGI GGATG 2 cut(s) 693, 778
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 4 cut(s) 88, 399, 704, 806
BseMII CTCAG 1 cut(s) 348
BseSI GKGCMC 1 cut(s) 684
BseXI GCAGC 4 cut(s) 67, 752, 824, 833
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 304, 759
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 8, 167, 380, 647
BshNI GGYRCC 2 cut(s) 506, 566
BshTI ACCGGT 1 cut(s) 710
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 9 cut(s) 47, 135, 168, 327, 353, 382, 444, 705, 711
BslI CCNNNNNNNGG 4 cut(s) 88, 399, 704, 806
BsmAI GTCTC 1 cut(s) 618
BsnI GGCC 4 cut(s) 8, 167, 380, 647
Bsp1286I GDGCHC 2 cut(s) 135, 684
Bsp143I GATC 5 cut(s) 60, 171, 300, 492, 843
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 6 cut(s) 72, 113, 122, 324, 404, 450
BspANI GGCC 4 cut(s) 8, 167, 380, 647
BspCNI CTCAG 1 cut(s) 347
BspLI GGNNCC 7 cut(s) 34, 108, 448, 508, 568, 648, 709
BspPI GGATC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 2 cut(s) 506, 566
BsrBI CCGCTC 2 cut(s) 74, 124
BsrFI RCCGGY 1 cut(s) 710
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 7 cut(s) 9, 309, 430, 453, 523, 534, 768
BssMI GATC 5 cut(s) 60, 171, 300, 492, 843
BssNI GRCGYC 4 cut(s) 146, 231, 507, 606
BssT1I CCWWGG 5 cut(s) 9, 309, 430, 523, 534
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 4 cut(s) 223, 262, 503, 589
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 4 cut(s) 146, 231, 507, 606
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 3 cut(s) 6, 117, 140
BstDEI CTNAG 1 cut(s) 334
BstDSI CCRYGG 2 cut(s) 453, 523
BstENI CCTNNNNNAGG 1 cut(s) 397
BstF5I GGATG 2 cut(s) 693, 778
BstH2I RGCGCY 2 cut(s) 510, 621
BstHHI GCGC 3 cut(s) 121, 509, 620
BstKTI GATC 5 cut(s) 63, 174, 303, 495, 846
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 5 cut(s) 60, 171, 300, 492, 843
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 8 cut(s) 121, 130, 139, 173, 449, 605, 818, 821
BstNI CCWGG 1 cut(s) 769
BstSCI CCNGG 6 cut(s) 45, 133, 167, 351, 380, 767
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 684
BstV1I GCAGC 4 cut(s) 67, 752, 824, 833
BstV2I GAAGAC 1 cut(s) 583
BsuRI GGCC 4 cut(s) 8, 167, 380, 647
BtgI CCRYGG 2 cut(s) 453, 523
BtgZI GCGATG 1 cut(s) 262
BtsCI GGATG 2 cut(s) 693, 778
BtsI GCAGTG 2 cut(s) 431, 547
BtsIMutI CAGTG 3 cut(s) 431, 547, 600
Cac8I GCNNGC 3 cut(s) 6, 117, 140
CaiI CAGNNNCTG 1 cut(s) 768
CfoI GCGC 3 cut(s) 121, 509, 620
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 4 cut(s) 165, 379, 646, 797
CseI GACGC 2 cut(s) 220, 614
CspAI ACCGGT 1 cut(s) 710
CspCI CAANNNNNGTGG 4 cut(s) 484, 519, 523, 558
CviAII CATG 6 cut(s) 83, 150, 421, 524, 632, 833
DdeI CTNAG 1 cut(s) 334
DinI GGCGCC 1 cut(s) 508
DpnI GATC 5 cut(s) 62, 173, 302, 494, 845
DpnII GATC 5 cut(s) 60, 171, 300, 492, 843
DrdI GACNNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 5 cut(s) 9, 309, 430, 523, 534
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 767
EcoT14I CCWWGG 5 cut(s) 9, 309, 430, 523, 534
EcoT38I GRGCYC 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
ErhI CCWWGG 5 cut(s) 9, 309, 430, 523, 534
FaeI CATG 6 cut(s) 86, 153, 424, 527, 635, 836
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FatI CATG 6 cut(s) 82, 149, 420, 523, 631, 832
FbaI TGATCA 1 cut(s) 843
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 7 cut(s) 56, 72, 122, 450, 741, 813, 822
FokI GGATG 2 cut(s) 680, 785
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 7 cut(s) 56, 72, 122, 450, 741, 813, 822
FspBI CTAG 1 cut(s) 726
GlaI GCGC 3 cut(s) 120, 508, 619
GluI GCNGC 7 cut(s) 56, 72, 122, 450, 741, 813, 822
GsaI CCCAGC 1 cut(s) 725
HaeII RGCGCY 2 cut(s) 510, 621
HaeIII GGCC 4 cut(s) 8, 167, 380, 647
HapII CCGG 9 cut(s) 47, 135, 168, 327, 353, 382, 444, 705, 711
HgaI GACGC 2 cut(s) 220, 614
HhaI GCGC 3 cut(s) 121, 509, 620
Hin1I GRCGYC 4 cut(s) 146, 231, 507, 606
Hin1II CATG 6 cut(s) 86, 153, 424, 527, 635, 836
Hin6I GCGC 3 cut(s) 119, 507, 618
HinP1I GCGC 3 cut(s) 119, 507, 618
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 3 cut(s) 330, 626, 730
HpaII CCGG 9 cut(s) 47, 135, 168, 327, 353, 382, 444, 705, 711
HphI GGTGA 4 cut(s) 202, 235, 254, 469
Hpy166II GTNNAC 3 cut(s) 316, 583, 682
Hpy188I TCNGA 2 cut(s) 639, 848
Hpy188III TCNNGA 3 cut(s) 217, 235, 490
Hpy8I GTNNAC 3 cut(s) 316, 583, 682
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 4 cut(s) 223, 262, 503, 589
HpyCH4IV ACGT 2 cut(s) 146, 191
HpyCH4V TGCA 7 cut(s) 4, 255, 321, 424, 682, 740, 821
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 130, 139, 173, 449, 605, 818, 821
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 2 cut(s) 146, 191
Hsp92I GRCGYC 4 cut(s) 146, 231, 507, 606
Hsp92II CATG 6 cut(s) 86, 153, 424, 527, 635, 836
HspAI GCGC 3 cut(s) 119, 507, 618
KasI GGCGCC 1 cut(s) 506
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 5 cut(s) 60, 171, 300, 492, 843
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 32, 106, 446
Lsp1109I GCAGC 4 cut(s) 67, 752, 824, 833
LweI GCATC 1 cut(s) 285
MaeI CTAG 1 cut(s) 726
MaeII ACGT 2 cut(s) 146, 191
MaeIII GTNAC 2 cut(s) 341, 358
MalI GATC 5 cut(s) 62, 173, 302, 494, 845
MbiI CCGCTC 2 cut(s) 74, 124
MboI GATC 5 cut(s) 60, 171, 300, 492, 843
MboII GAAGA 6 cut(s) 29, 118, 295, 478, 583, 742
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 2 cut(s) 135, 684
MluCI AATT 2 cut(s) 558, 657
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 2 cut(s) 620, 739
MmeI TCCRAC 1 cut(s) 775
MnlI CCTC 3 cut(s) 7, 45, 82
MseI TTAA 1 cut(s) 717
MslI CAYNNNNRTG 1 cut(s) 419
MspI CCGG 9 cut(s) 47, 135, 168, 327, 353, 382, 444, 705, 711
MspR9I CCNGG 6 cut(s) 47, 135, 169, 353, 382, 769
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 769
MwoI GCNNNNNNNGC 8 cut(s) 121, 130, 139, 173, 449, 605, 818, 821
NarI GGCGCC 1 cut(s) 507
NciI CCSGG 5 cut(s) 47, 135, 169, 353, 382
NcoI CCATGG 1 cut(s) 523
NdeII GATC 5 cut(s) 60, 171, 300, 492, 843
NlaIII CATG 6 cut(s) 86, 153, 424, 527, 635, 836
NlaIV GGNNCC 7 cut(s) 34, 108, 448, 508, 568, 648, 709
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 2 cut(s) 341, 358
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 152, 180
PfeI GAWTC 1 cut(s) 330
PflMI CCANNNNNTGG 1 cut(s) 806
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 710
PkrI GCNGC 7 cut(s) 57, 73, 123, 451, 742, 814, 823
Ple19I CGATCG 1 cut(s) 63
PleI GAGTC 2 cut(s) 620, 738
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 2 cut(s) 620, 738
Psp6I CCWGG 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 721
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 7 cut(s) 34, 108, 448, 508, 568, 648, 709
PspPI GGNCC 4 cut(s) 165, 379, 646, 797
PstNI CAGNNNCTG 1 cut(s) 768
PvuI CGATCG 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 7 cut(s) 56, 72, 122, 450, 741, 813, 822
Sau3AI GATC 5 cut(s) 60, 171, 300, 492, 843
Sau96I GGNCC 4 cut(s) 165, 379, 646, 797
SchI GAGTC 2 cut(s) 620, 739
ScrFI CCNGG 6 cut(s) 47, 135, 169, 353, 382, 769
SduI GDGCHC 2 cut(s) 135, 684
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 438
SfoI GGCGCC 1 cut(s) 508
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 419
Sse9I AATT 2 cut(s) 558, 657
SsiI CCGC 6 cut(s) 72, 113, 122, 324, 404, 450
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 6 cut(s) 45, 133, 167, 351, 380, 767
StyI CCWWGG 5 cut(s) 9, 309, 430, 523, 534
TaaI ACNGT 4 cut(s) 223, 262, 503, 589
TaiI ACGT 2 cut(s) 149, 194
TaqI TCGA 2 cut(s) 129, 489
TasI AATT 2 cut(s) 558, 657
TauI GCSGC 3 cut(s) 74, 124, 452
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 3 cut(s) 431, 547, 607
TseFI GTSAC 2 cut(s) 341, 358
TseI GCWGC 4 cut(s) 55, 740, 812, 821
Tsp45I GTSAC 2 cut(s) 341, 358
TspDTI ATGAA 1 cut(s) 793
TspRI CASTG 3 cut(s) 431, 547, 607
Van91I CCANNNNNTGG 1 cut(s) 806
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 797
XagI CCTNNNNNAGG 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 1 cut(s) 726
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.