Rorug06G0066500

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011596
Physical Location & Seq
Forward (+)
8802204 .. 8803282
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0066500.1

Sequence Viewer

Length: 687 bp
ATGGCTCGCTTTCTCCTTTCATTGGTCTTTGCTTCTCTCTTAGCCCTAGCTACTGTCCATGAAACTCAAGCTATTGAGTACATCGTCGAGAACCGGGCTCTCAGCACCCCTGGCGGAGTCCGTTTTAGAGACGAGCTGGGCGTGGACTACACCAAGCAAAGAATGAGAGACGCGACTGAATTCATTTGGAACCTCTTTAAGCAAACTACAGCTGCAGAGAGAAGGGACACGCCGCGCATAAGCTTGTTCGTCGAAGAGAGAGAAGGCATTGCAGTCTCCAGCAATGACATTATTCATGTTTCAGCCAAGTACATCGAGGACCTATCGGCAGCAAACCTCAAAAACGACTTCAATTGGGTACTTTACCATGAGATCACACACTCATTGCAGTGGAATGGAAACGGTCAAGCTCCTACCGGAGTGGTAGAAGGGATTGCTGAGTTTGTGACCTTGAAGTCTGGTTTTATACCCTCCTCGTTTGCAAAGCCCGGTGATGGTACCAGTTGGGACGAGGGTTACAGCGTAACTGCACGGTTCCTGGATTATTGCAATGGCCTTAAAGACGGGTTTGTGGCAGAACTTAACAAGAAGATGAGAGATGGTTACAGTAACGATTTCTTTAATCAGTTGCTCGGGAAGACGGTCGAACAGCTGTGGACCGACTACAAAGCCAAATATGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

228

Amino Acids

25.74

Weight (kDa)

5.3

Isoelectric Point (pI)

22.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 24 - 222 1.3e-64 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 454
Acc65I GGTACC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 497
AccII CGCG 2 cut(s) 173, 235
AciI CCGC 2 cut(s) 114, 233
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 4 cut(s) 80, 311, 360, 499
AfiI CCNNNNNNNGG 2 cut(s) 22, 494
AgsI TTSAA 2 cut(s) 352, 454
AjnI CCWGG 2 cut(s) 109, 537
AluBI AGCT 7 cut(s) 50, 71, 136, 212, 243, 410, 652
AluI AGCT 7 cut(s) 50, 71, 136, 212, 243, 410, 652
Alw26I GTCTC 3 cut(s) 123, 162, 280
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 1 cut(s) 553
ApeKI GCWGC 2 cut(s) 212, 329
ApoI RAATTY 1 cut(s) 179
Asp718I GGTACC 1 cut(s) 497
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 2 cut(s) 319, 657
AsuC2I CCSGG 2 cut(s) 95, 489
AsuHPI GGTGA 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 319, 657
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 644
BbvI GCAGC 2 cut(s) 199, 341
BccI CCATC 2 cut(s) 488, 593
BciT130I CCWGG 2 cut(s) 111, 539
BcnI CCSGG 2 cut(s) 95, 489
BcoDI GTCTC 3 cut(s) 123, 162, 280
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 2 cut(s) 207, 213
BisI GCNGC 3 cut(s) 213, 233, 330
BlsI GCNGC 3 cut(s) 214, 234, 331
Bme1390I CCNGG 4 cut(s) 95, 111, 489, 539
Bme18I GGWCC 2 cut(s) 319, 657
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 319, 657
BmiI GGNNCC 3 cut(s) 191, 499, 536
BmrFI CCNGG 4 cut(s) 95, 111, 489, 539
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 95, 489
BsaJI CCNNGG 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 416
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 494
Bse1I ACTGG 1 cut(s) 501
Bse3DI GCAATG 4 cut(s) 267, 289, 383, 556
BseBI CCWGG 2 cut(s) 111, 539
BseDI CCNNGG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 22, 494
BseMI GCAATG 4 cut(s) 267, 289, 383, 556
BseMII CTCAG 2 cut(s) 115, 429
BseNI ACTGG 1 cut(s) 501
BseRI GAGGAG 1 cut(s) 463
BseXI GCAGC 2 cut(s) 199, 341
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 2 cut(s) 173, 235
Bsh1285I CGRYCG 1 cut(s) 645
BshFI GGCC 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 497
BsiEI CGRYCG 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 3 cut(s) 94, 417, 489
BslFI GGGAC 2 cut(s) 239, 521
BslI CCNNNNNNNGG 2 cut(s) 22, 494
BsmAI GTCTC 3 cut(s) 123, 162, 280
BsmBI CGTCTC 2 cut(s) 123, 162
BsmFI GGGAC 2 cut(s) 239, 521
BsnI GGCC 1 cut(s) 555
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 1 cut(s) 372
BspACI CCGC 2 cut(s) 114, 233
BspANI GGCC 1 cut(s) 555
BspCNI CTCAG 2 cut(s) 114, 430
BspFNI CGCG 2 cut(s) 173, 235
BspLI GGNNCC 3 cut(s) 191, 499, 536
BspMAI CTGCAG 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 497
BsrDI GCAATG 4 cut(s) 267, 289, 383, 556
BsrI ACTGG 1 cut(s) 501
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 111, 539
Bst4CI ACNGT 5 cut(s) 55, 404, 534, 608, 643
Bst6I CTCTTC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 3 cut(s) 40, 101, 438
BstFNI CGCG 2 cut(s) 173, 235
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 1 cut(s) 375
BstMAI GTCTC 3 cut(s) 123, 162, 280
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 1 cut(s) 645
BstMWI GCNNNNNNNGC 2 cut(s) 111, 677
BstNI CCWGG 2 cut(s) 111, 539
BstSCI CCNGG 4 cut(s) 93, 109, 487, 537
BstSFI CTRYAG 2 cut(s) 207, 213
BstUI CGCG 2 cut(s) 173, 235
BstV1I GCAGC 2 cut(s) 199, 341
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 1 cut(s) 555
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 1 cut(s) 395
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 319, 657
CseI GACGC 1 cut(s) 179
Csp6I GTAC 4 cut(s) 79, 310, 359, 498
CviAII CATG 3 cut(s) 59, 296, 368
CviQI GTAC 4 cut(s) 79, 310, 359, 498
DdeI CTNAG 3 cut(s) 40, 101, 438
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
EciI GGCGGA 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 319, 657
Eco88I CYCGRG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 109, 537
EcoT38I GRGCYC 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 123, 162
FaeI CATG 3 cut(s) 62, 299, 371
FaiI YATR 6 cut(s) 60, 239, 297, 369, 467, 678
FaqI GGGAC 2 cut(s) 239, 521
FatI CATG 3 cut(s) 58, 295, 367
Fnu4HI GCNGC 3 cut(s) 213, 233, 330
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 3 cut(s) 213, 233, 330
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 236
GluI GCNGC 3 cut(s) 213, 233, 330
GsaI CCCAGC 1 cut(s) 140
GsuI CTGGAG 1 cut(s) 262
HaeIII GGCC 1 cut(s) 555
HapII CCGG 3 cut(s) 94, 417, 489
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 237
Hin1II CATG 3 cut(s) 62, 299, 371
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 241
HinfI GANTC 1 cut(s) 117
HpaII CCGG 3 cut(s) 94, 417, 489
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 2 cut(s) 145, 657
Hpy188III TCNNGA 2 cut(s) 88, 634
Hpy8I GTNNAC 2 cut(s) 145, 657
Hpy99I CGWCG 2 cut(s) 89, 254
HpyAV CCTTC 3 cut(s) 216, 257, 422
HpyCH4III ACNGT 5 cut(s) 55, 404, 534, 608, 643
HpyCH4V TGCA 7 cut(s) 215, 272, 388, 482, 530, 549, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 677
HpyF3I CTNAG 3 cut(s) 40, 101, 438
Hsp92II CATG 3 cut(s) 62, 299, 371
HspAI GCGC 1 cut(s) 235
KpnI GGTACC 1 cut(s) 501
Kzo9I GATC 1 cut(s) 372
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 2 cut(s) 199, 341
MaeI CTAG 1 cut(s) 47
MaeIII GTNAC 5 cut(s) 445, 515, 523, 602, 608
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MboII GAAGA 3 cut(s) 266, 601, 649
MfeI CAATTG 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 2 cut(s) 179, 352
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 6 cut(s) 203, 310, 347, 481, 484, 505
MseI TTAA 4 cut(s) 198, 558, 582, 621
MslI CAYNNNNRTG 1 cut(s) 388
MspA1I CMGCKG 2 cut(s) 212, 652
MspI CCGG 3 cut(s) 94, 417, 489
MspR9I CCNGG 4 cut(s) 95, 111, 489, 539
MunI CAATTG 1 cut(s) 352
MvaI CCWGG 2 cut(s) 111, 539
MvnI CGCG 2 cut(s) 173, 235
MwoI GCNNNNNNNGC 2 cut(s) 111, 677
NciI CCSGG 2 cut(s) 95, 489
NdeII GATC 1 cut(s) 372
NlaIII CATG 3 cut(s) 62, 299, 371
NlaIV GGNNCC 3 cut(s) 191, 499, 536
NmuCI GTSAC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 3 cut(s) 214, 234, 331
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 109, 537
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 2 cut(s) 109, 537
PspN4I GGNNCC 3 cut(s) 191, 499, 536
PspPI GGNCC 2 cut(s) 319, 657
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 1 cut(s) 217
PvuII CAGCTG 2 cut(s) 212, 652
RsaI GTAC 4 cut(s) 80, 311, 360, 499
RsaNI GTAC 4 cut(s) 79, 310, 359, 498
RseI CAYNNNNRTG 1 cut(s) 388
SaqAI TTAA 4 cut(s) 198, 558, 582, 621
SatI GCNGC 3 cut(s) 213, 233, 330
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 2 cut(s) 319, 657
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 4 cut(s) 95, 111, 489, 539
SduI GDGCHC 1 cut(s) 100
SfcI CTRYAG 2 cut(s) 207, 213
SinI GGWCC 2 cut(s) 319, 657
SmiMI CAYNNNNRTG 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 2 cut(s) 179, 352
SsiI CCGC 2 cut(s) 114, 233
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 4 cut(s) 93, 109, 487, 537
TaaI ACNGT 5 cut(s) 55, 404, 534, 608, 643
TaqI TCGA 4 cut(s) 87, 252, 315, 645
TaqII GACCGA 1 cut(s) 674
TasI AATT 2 cut(s) 179, 352
TatI WGTACW 2 cut(s) 78, 309
TauI GCSGC 1 cut(s) 235
Tru1I TTAA 4 cut(s) 198, 558, 582, 621
Tru9I TTAA 4 cut(s) 198, 558, 582, 621
TscAI CASTG 1 cut(s) 395
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 2 cut(s) 212, 329
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 4 cut(s) 9, 75, 172, 284
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 319, 657
XapI RAATTY 1 cut(s) 179
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.