Rorug04G0079200

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011596
Physical Location & Seq
Forward (+)
12140666 .. 12141091
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0079200.1

Sequence Viewer

Length: 426 bp
ATGACGTTTTCCTCCGCTGCTTCTCGATTGGGTTTGGGGATAAGGACTGCTTCTTCCTCCACCGCCAGCAAAATCGTGGAGCCTCGAGGCCACATTCTAAGGGCCACTGGCCGGAAGGACCACCACAGCAAGGTCTGCACCGCCAAAGGCCCCAGGGACCATCGCGTCCGGCTCGCCGCCCACACCGCCATTCAATTCTACGACGTGCAAGACCGGCTCGGATACGACCAGCCTAGCAAGGCCGTCGATTGGCTCATCAAGAAAGCCAAGGCCGCAATCGACGAACTGCCGCCGTGGAACCCGAACTCAATTTCTGTTCAAACAACATGCATCTTCTCCGACGGTGCCGATATCCGCCGCGCAGGACATGCAGAACGCGAGCCTCCATTTCTCAATGGTGGAGGCTCCGAGTTCTTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

141

Amino Acids

15.4

Weight (kDa)

9.55

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCP PF03634 37 - 101 7e-29 TCP family transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AbsI CCTCGAGG 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 344
AccII CGCG 3 cut(s) 165, 360, 378
AciI CCGC 9 cut(s) 15, 63, 141, 177, 186, 273, 290, 355, 358
AcoI YGGCCR 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 111, 130, 249
AgsI TTSAA 2 cut(s) 194, 320
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 6 cut(s) 88, 102, 109, 148, 240, 270
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 4 cut(s) 102, 118, 149, 157
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 2 cut(s) 118, 157
BanI GGYRCC 1 cut(s) 344
BbvI GCAGC 1 cut(s) 4
BccI CCATC 1 cut(s) 168
BceAI ACGGC 2 cut(s) 227, 277
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 154
BciVI GTATCC 1 cut(s) 215
BfaI CTAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 215
BisI GCNGC 5 cut(s) 18, 177, 273, 290, 358
BlsI GCNGC 5 cut(s) 19, 178, 274, 291, 359
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 2 cut(s) 118, 157
BmeT110I CYCGRG 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 4 cut(s) 102, 118, 149, 157
BmiI GGNNCC 6 cut(s) 81, 151, 158, 299, 346, 406
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 152, 153, 267, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 130, 249
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 4 cut(s) 152, 153, 267, 293
BseLI CCNNNNNNNGG 3 cut(s) 111, 130, 249
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 3 cut(s) 165, 360, 378
BshFI GGCC 6 cut(s) 90, 104, 111, 150, 242, 272
BshNI GGYRCC 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 3 cut(s) 112, 169, 214
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 111, 130, 249
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 6 cut(s) 90, 104, 111, 150, 242, 272
BsoBI CYCGRG 1 cut(s) 84
BspACI CCGC 9 cut(s) 15, 63, 141, 177, 186, 273, 290, 355, 358
BspANI GGCC 6 cut(s) 90, 104, 111, 150, 242, 272
BspFNI CGCG 3 cut(s) 165, 360, 378
BspLI GGNNCC 6 cut(s) 81, 151, 158, 299, 346, 406
BspT107I GGYRCC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 4 cut(s) 152, 153, 267, 293
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 344
BstAPI GCANNNNNTGC 2 cut(s) 135, 368
BstC8I GCNNGC 3 cut(s) 67, 174, 380
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 293
BstFNI CGCG 3 cut(s) 165, 360, 378
BstHHI GCGC 1 cut(s) 362
BstMWI GCNNNNNNNGC 5 cut(s) 135, 185, 214, 272, 368
BstNI CCWGG 1 cut(s) 154
BstNSI RCATGY 2 cut(s) 330, 371
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 3 cut(s) 165, 360, 378
BstV1I GCAGC 1 cut(s) 4
BsuI GTATCC 1 cut(s) 215
BsuRI GGCC 6 cut(s) 90, 104, 111, 150, 242, 272
BtgI CCRYGG 1 cut(s) 293
BtgZI GCGATG 1 cut(s) 146
BtrI CACGTC 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 67, 174, 380
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 4 cut(s) 102, 118, 149, 157
CseI GACGC 1 cut(s) 154
CviAII CATG 2 cut(s) 327, 368
DdeI CTNAG 1 cut(s) 98
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 109
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 267
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 118, 157
Eco88I CYCGRG 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 152
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 2 cut(s) 330, 371
FaiI YATR 2 cut(s) 328, 369
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 1 cut(s) 170
FatI CATG 2 cut(s) 326, 367
Fnu4HI GCNGC 5 cut(s) 18, 177, 273, 290, 358
Fsp4HI GCNGC 5 cut(s) 18, 177, 273, 290, 358
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 361
GluI GCNGC 5 cut(s) 18, 177, 273, 290, 358
HaeIII GGCC 6 cut(s) 90, 104, 111, 150, 242, 272
HapII CCGG 3 cut(s) 112, 169, 214
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 362
Hin1II CATG 2 cut(s) 330, 371
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HpaII CCGG 3 cut(s) 112, 169, 214
Hpy188I TCNGA 3 cut(s) 221, 340, 409
Hpy188III TCNNGA 2 cut(s) 24, 259
Hpy99I CGWCG 4 cut(s) 206, 248, 284, 344
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 2 cut(s) 5, 204
HpyCH4V TGCA 4 cut(s) 138, 208, 330, 371
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 185, 214, 272, 368
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 2 cut(s) 5, 204
Hsp92II CATG 2 cut(s) 330, 371
HspAI GCGC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 79, 410
LpnPI CCDG 9 cut(s) 79, 93, 125, 139, 166, 182, 227, 242, 348
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 234
MaeII ACGT 2 cut(s) 5, 204
MboII GAAGA 2 cut(s) 45, 325
MluCI AATT 2 cut(s) 194, 309
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 6 cut(s) 22, 67, 80, 93, 393, 395
Mph1103I ATGCAT 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 112, 169, 214
MspR9I CCNGG 1 cut(s) 154
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 3 cut(s) 165, 360, 378
MwoI GCNNNNNNNGC 5 cut(s) 135, 185, 214, 272, 368
NlaIII CATG 2 cut(s) 330, 371
NlaIV GGNNCC 6 cut(s) 81, 151, 158, 299, 346, 406
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 2 cut(s) 330, 371
PaeR7I CTCGAG 1 cut(s) 84
PasI CCCWGGG 1 cut(s) 153
PkrI GCNGC 5 cut(s) 19, 178, 274, 291, 359
Psp6I CCWGG 1 cut(s) 152
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 6 cut(s) 81, 151, 158, 299, 346, 406
PspPI GGNCC 4 cut(s) 102, 118, 149, 157
PspXI VCTCGAGB 1 cut(s) 84
SatI GCNGC 5 cut(s) 18, 177, 273, 290, 358
Sau96I GGNCC 4 cut(s) 102, 118, 149, 157
ScrFI CCNGG 1 cut(s) 154
SetI ASST 3 cut(s) 8, 135, 207
SfaNI GCATC 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 84
SinI GGWCC 2 cut(s) 118, 157
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 2 cut(s) 194, 309
SsiI CCGC 9 cut(s) 15, 63, 141, 177, 186, 273, 290, 355, 358
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 2 cut(s) 8, 207
TaqI TCGA 4 cut(s) 25, 85, 246, 279
TasI AATT 2 cut(s) 194, 309
TauI GCSGC 4 cut(s) 179, 275, 292, 360
TscAI CASTG 1 cut(s) 112
TseI GCWGC 1 cut(s) 17
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 118, 157
XceI RCATGY 2 cut(s) 330, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 84
XspI CTAG 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.