Leucine aminopeptidase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 4254 |
| AarI | CACCTGC | 1 cut(s) | 1859 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 894, 3420 |
| Acc36I | ACCTGC | 1 cut(s) | 1859 |
| Acc65I | GGTACC | 1 cut(s) | 2592 |
| AccB1I | GGYRCC | 4 cut(s) | 782, 2499, 2592, 4277 |
| AccI | GTMKAC | 6 cut(s) | 772, 886, 1114, 1486, 1691, 2818 |
| AccII | CGCG | 1 cut(s) | 1110 |
| AciI | CCGC | 6 cut(s) | 89, 153, 165, 444, 1110, 3983 |
| AclI | AACGTT | 1 cut(s) | 4584 |
| AclWI | GGATC | 6 cut(s) | 129, 664, 2853, 4012, 4157, 4635 |
| AcoI | YGGCCR | 3 cut(s) | 1629, 1679, 3804 |
| AcsI | RAATTY | 8 cut(s) | 105, 345, 368, 2216, 2261, 3797, 4144, 4349 |
| AcyI | GRCGYC | 1 cut(s) | 4278 |
| AflII | CTTAAG | 2 cut(s) | 2450, 2768 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2708, 2740, 3875, 3907 |
| AleI | CACNNNNGTG | 1 cut(s) | 4488 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1446, 1478, 2094, 2126 |
| Alw21I | GWGCWC | 3 cut(s) | 622, 2000, 2350 |
| Alw26I | GTCTC | 8 cut(s) | 904, 942, 1915, 2026, 2170, 2293, 2389, 2401 |
| AlwI | GGATC | 6 cut(s) | 129, 664, 2853, 4012, 4157, 4635 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2928 |
| Ama87I | CYCGRG | 2 cut(s) | 2442, 2597 |
| ApeKI | GCWGC | 7 cut(s) | 1242, 1850, 1910, 2928, 3404, 3617, 4685 |
| ApoI | RAATTY | 8 cut(s) | 105, 345, 368, 2216, 2261, 3797, 4144, 4349 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 2025, 2029, 2057, 2061, 4097, 4129 |
| AseI | ATTAAT | 1 cut(s) | 3666 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 345, 1020, 1653, 4584, 4596 |
| Asp718I | GGTACC | 1 cut(s) | 2592 |
| AspLEI | GCGC | 3 cut(s) | 1110, 2437, 4280 |
| AspS9I | GGNCC | 7 cut(s) | 866, 1193, 2446, 3292, 3896, 4158, 4558 |
| AsuC2I | CCSGG | 3 cut(s) | 2443, 2444, 3446 |
| AvaI | CYCGRG | 2 cut(s) | 2442, 2597 |
| AvaII | GGWCC | 5 cut(s) | 1193, 2446, 3292, 3896, 4158 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1079, 1112 |
| BalI | TGGCCA | 2 cut(s) | 1631, 3806 |
| BanI | GGYRCC | 4 cut(s) | 782, 2499, 2592, 4277 |
| BanII | GRGCYC | 1 cut(s) | 2000 |
| BauI | CACGAG | 2 cut(s) | 1289, 2851 |
| BbsI | GAAGAC | 2 cut(s) | 908, 2609 |
| Bbv12I | GWGCWC | 3 cut(s) | 622, 2000, 2350 |
| BbvCI | CCTCAGC | 1 cut(s) | 966 |
| BbvI | GCAGC | 7 cut(s) | 1254, 1862, 1897, 2915, 3416, 3629, 4697 |
| BceAI | ACGGC | 2 cut(s) | 957, 4265 |
| BcnI | CCSGG | 3 cut(s) | 2443, 2444, 3446 |
| BcoDI | GTCTC | 8 cut(s) | 904, 942, 1915, 2026, 2170, 2293, 2389, 2401 |
| BfaI | CTAG | 8 cut(s) | 767, 2012, 2169, 2292, 2400, 2864, 3159, 4418 |
| BfmI | CTRYAG | 4 cut(s) | 1091, 1848, 2429, 2656 |
| BfoI | RGCGCY | 1 cut(s) | 4281 |
| BfrI | CTTAAG | 2 cut(s) | 2450, 2768 |
| BfuAI | ACCTGC | 1 cut(s) | 1859 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1628 |
| BglII | AGATCT | 1 cut(s) | 2792 |
| BisI | GCNGC | 7 cut(s) | 1243, 1851, 1911, 2929, 3405, 3618, 4686 |
| BlsI | GCNGC | 7 cut(s) | 1244, 1852, 1912, 2930, 3406, 3619, 4687 |
| BmcAI | AGTACT | 2 cut(s) | 2073, 3603 |
| Bme18I | GGWCC | 5 cut(s) | 1193, 2446, 3292, 3896, 4158 |
| BmeT110I | CYCGRG | 2 cut(s) | 2442, 2597 |
| BmgT120I | GGNCC | 7 cut(s) | 866, 1193, 2446, 3292, 3896, 4158, 4558 |
| BmrI | ACTGGG | 2 cut(s) | 3113, 3271 |
| BmuI | ACTGGG | 2 cut(s) | 3113, 3271 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3894 |
| BpiI | GAAGAC | 2 cut(s) | 908, 2609 |
| BpmI | CTGGAG | 3 cut(s) | 3517, 4008, 4679 |
| Bpu10I | CCTNAGC | 2 cut(s) | 966, 3551 |
| BpuEI | CTTGAG | 3 cut(s) | 606, 2654, 3318 |
| BpuMI | CCSGG | 3 cut(s) | 2443, 2444, 3446 |
| BsaAI | YACGTR | 1 cut(s) | 2822 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2874 |
| BsaHI | GRCGYC | 1 cut(s) | 4278 |
| BsaI | GGTCTC | 5 cut(s) | 1915, 2170, 2293, 2389, 2401 |
| BsaWI | WCCGGW | 1 cut(s) | 1231 |
| Bse118I | RCCGGY | 1 cut(s) | 2016 |
| Bse3DI | GCAATG | 1 cut(s) | 3379 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2874 |
| BseJI | GATNNNNATC | 1 cut(s) | 2874 |
| BseMI | GCAATG | 1 cut(s) | 3379 |
| BseRI | GAGGAG | 7 cut(s) | 155, 775, 3514, 3626, 3944, 3957, 4625 |
| BseXI | GCAGC | 7 cut(s) | 1254, 1862, 1897, 2915, 3416, 3629, 4697 |
| BseYI | CCCAGC | 2 cut(s) | 2693, 3555 |
| Bsh1236I | CGCG | 1 cut(s) | 1110 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1114 |
| BshNI | GGYRCC | 4 cut(s) | 782, 2499, 2592, 4277 |
| BsiEI | CGRYCG | 1 cut(s) | 1114 |
| BsiHKAI | GWGCWC | 3 cut(s) | 622, 2000, 2350 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2442, 2597 |
| BsiSI | CCGG | 4 cut(s) | 1232, 2017, 2443, 3446 |
| BslFI | GGGAC | 4 cut(s) | 307, 2459, 4171, 4301 |
| BsmAI | GTCTC | 8 cut(s) | 904, 942, 1915, 2026, 2170, 2293, 2389, 2401 |
| BsmBI | CGTCTC | 1 cut(s) | 2026 |
| BsmFI | GGGAC | 4 cut(s) | 307, 2459, 4171, 4301 |
| BsmI | GAATGC | 5 cut(s) | 414, 459, 1614, 4596, 4598 |
| Bso31I | GGTCTC | 5 cut(s) | 1915, 2170, 2293, 2389, 2401 |
| BsoBI | CYCGRG | 2 cut(s) | 2442, 2597 |
| Bsp1286I | GDGCHC | 3 cut(s) | 622, 2000, 2350 |
| Bsp1407I | TGTACA | 2 cut(s) | 2827, 3454 |
| Bsp19I | CCATGG | 1 cut(s) | 4212 |
| BspACI | CCGC | 6 cut(s) | 89, 153, 165, 444, 1110, 3983 |
| BspFNI | CGCG | 1 cut(s) | 1110 |
| BspHI | TCATGA | 2 cut(s) | 3424, 4399 |
| BspMAI | CTGCAG | 3 cut(s) | 1095, 1852, 2660 |
| BspMI | ACCTGC | 1 cut(s) | 1859 |
| BspPI | GGATC | 6 cut(s) | 129, 664, 2853, 4012, 4157, 4635 |
| BspT107I | GGYRCC | 4 cut(s) | 782, 2499, 2592, 4277 |
| BspTI | CTTAAG | 2 cut(s) | 2450, 2768 |
| BspTNI | GGTCTC | 5 cut(s) | 1915, 2170, 2293, 2389, 2401 |
| BsrDI | GCAATG | 1 cut(s) | 3379 |
| BsrFI | RCCGGY | 1 cut(s) | 2016 |
| BsrGI | TGTACA | 2 cut(s) | 2827, 3454 |
| BssAI | RCCGGY | 1 cut(s) | 2016 |
| BssNAI | GTATAC | 1 cut(s) | 2819 |
| BssNI | GRCGYC | 1 cut(s) | 4278 |
| BssSI | CACGAG | 2 cut(s) | 1289, 2851 |
| BssT1I | CCWWGG | 6 cut(s) | 439, 3782, 4153, 4212, 4466, 4486 |
| Bst1107I | GTATAC | 1 cut(s) | 2819 |
| Bst2BI | CACGAG | 2 cut(s) | 1289, 2851 |
| Bst6I | CTCTTC | 8 cut(s) | 684, 735, 752, 2024, 2156, 2835, 3765, 4443 |
| BstACI | GRCGYC | 1 cut(s) | 4278 |
| BstAFI | CTTAAG | 2 cut(s) | 2450, 2768 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1182, 2664, 4682, 4694 |
| BstAUI | TGTACA | 2 cut(s) | 2827, 3454 |
| BstBAI | YACGTR | 1 cut(s) | 2822 |
| BstC8I | GCNNGC | 6 cut(s) | 995, 2018, 3370, 3402, 4002, 4622 |
| BstDSI | CCRYGG | 1 cut(s) | 4212 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 2453, 2672, 4490 |
| BstFNI | CGCG | 1 cut(s) | 1110 |
| BstH2I | RGCGCY | 1 cut(s) | 4281 |
| BstHHI | GCGC | 3 cut(s) | 1110, 2437, 4280 |
| BstMAI | GTCTC | 8 cut(s) | 904, 942, 1915, 2026, 2170, 2293, 2389, 2401 |
| BstMCI | CGRYCG | 1 cut(s) | 1114 |
| BstNSI | RCATGY | 4 cut(s) | 3351, 3372, 3404, 4004 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3894 |
| BstSFI | CTRYAG | 4 cut(s) | 1091, 1848, 2429, 2656 |
| BstUI | CGCG | 1 cut(s) | 1110 |
| BstV1I | GCAGC | 7 cut(s) | 1254, 1862, 1897, 2915, 3416, 3629, 4697 |
| BstV2I | GAAGAC | 2 cut(s) | 908, 2609 |
| BstX2I | RGATCY | 5 cut(s) | 121, 2792, 2845, 4017, 4640 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1001 |
| BstYI | RGATCY | 5 cut(s) | 121, 2792, 2845, 4017, 4640 |
| BstZ17I | GTATAC | 1 cut(s) | 2819 |
| BtgI | CCRYGG | 1 cut(s) | 4212 |
| BtgZI | GCGATG | 2 cut(s) | 2103, 3895 |
| BtsI | GCAGTG | 1 cut(s) | 3013 |
| BtsIMutI | CAGTG | 8 cut(s) | 727, 2218, 2341, 2513, 3013, 3297, 3493, 4676 |
| BveI | ACCTGC | 1 cut(s) | 1859 |
| Cac8I | GCNNGC | 6 cut(s) | 995, 2018, 3370, 3402, 4002, 4622 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2928 |
| CciI | TCATGA | 2 cut(s) | 3424, 4399 |
| CfoI | GCGC | 3 cut(s) | 1110, 2437, 4280 |
| Cfr10I | RCCGGY | 1 cut(s) | 2016 |
| Cfr13I | GGNCC | 7 cut(s) | 866, 1193, 2446, 3292, 3896, 4158, 4558 |
| Cfr9I | CCCGGG | 1 cut(s) | 2442 |
| CseI | GACGC | 1 cut(s) | 4006 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 715, 750 |
| DinI | GGCGCC | 1 cut(s) | 4279 |
| DraI | TTTAAA | 1 cut(s) | 2547 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 894, 3420 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 894, 3420 |
| EaeI | YGGCCR | 3 cut(s) | 1629, 1679, 3804 |
| Eam1104I | CTCTTC | 8 cut(s) | 684, 735, 752, 2024, 2156, 2835, 3765, 4443 |
| EarI | CTCTTC | 8 cut(s) | 684, 735, 752, 2024, 2156, 2835, 3765, 4443 |
| Ecl136II | GAGCTC | 1 cut(s) | 1998 |
| Eco130I | CCWWGG | 6 cut(s) | 439, 3782, 4153, 4212, 4466, 4486 |
| Eco147I | AGGCCT | 6 cut(s) | 438, 462, 500, 1813, 2167, 2290 |
| Eco24I | GRGCYC | 1 cut(s) | 2000 |
| Eco31I | GGTCTC | 5 cut(s) | 1915, 2170, 2293, 2389, 2401 |
| Eco47I | GGWCC | 5 cut(s) | 1193, 2446, 3292, 3896, 4158 |
| Eco53kI | GAGCTC | 1 cut(s) | 1998 |
| Eco88I | CYCGRG | 2 cut(s) | 2442, 2597 |
| EcoICRI | GAGCTC | 1 cut(s) | 1998 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 2453, 2672, 4490 |
| EcoO109I | RGGNCCY | 3 cut(s) | 2446, 3292, 3896 |
| EcoRI | GAATTC | 1 cut(s) | 2216 |
| EcoT14I | CCWWGG | 6 cut(s) | 439, 3782, 4153, 4212, 4466, 4486 |
| EcoT22I | ATGCAT | 4 cut(s) | 553, 1460, 2082, 4598 |
| EcoT38I | GRGCYC | 1 cut(s) | 2000 |
| EgeI | GGCGCC | 1 cut(s) | 4279 |
| EheI | GGCGCC | 1 cut(s) | 4279 |
| ErhI | CCWWGG | 6 cut(s) | 439, 3782, 4153, 4212, 4466, 4486 |
| Esp3I | CGTCTC | 1 cut(s) | 2026 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1005, 1037, 3875, 3907 |
| FaqI | GGGAC | 4 cut(s) | 307, 2459, 4171, 4301 |
| FauI | CCCGC | 1 cut(s) | 82 |
| FauNDI | CATATG | 1 cut(s) | 4270 |
| FblI | GTMKAC | 6 cut(s) | 772, 886, 1114, 1486, 1691, 2818 |
| Fnu4HI | GCNGC | 7 cut(s) | 1243, 1851, 1911, 2929, 3405, 3618, 4686 |
| FriOI | GRGCYC | 1 cut(s) | 2000 |
| FseI | GGCCGGCC | 1 cut(s) | 2020 |
| Fsp4HI | GCNGC | 7 cut(s) | 1243, 1851, 1911, 2929, 3405, 3618, 4686 |
| FspBI | CTAG | 8 cut(s) | 767, 2012, 2169, 2292, 2400, 2864, 3159, 4418 |
| GlaI | GCGC | 3 cut(s) | 1109, 2436, 4279 |
| GluI | GCNGC | 7 cut(s) | 1243, 1851, 1911, 2929, 3405, 3618, 4686 |
| GsaI | CCCAGC | 2 cut(s) | 2697, 3559 |
| GsuI | CTGGAG | 3 cut(s) | 3517, 4008, 4679 |
| HaeII | RGCGCY | 1 cut(s) | 4281 |
| HapII | CCGG | 4 cut(s) | 1232, 2017, 2443, 3446 |
| HgaI | GACGC | 1 cut(s) | 4006 |
| HhaI | GCGC | 3 cut(s) | 1110, 2437, 4280 |
| Hin1I | GRCGYC | 1 cut(s) | 4278 |
| Hin6I | GCGC | 3 cut(s) | 1108, 2435, 4278 |
| HinP1I | GCGC | 3 cut(s) | 1108, 2435, 4278 |
| HincII | GTYRAC | 9 cut(s) | 773, 887, 1115, 1285, 1487, 1692, 3029, 4320, 4605 |
| HindII | GTYRAC | 9 cut(s) | 773, 887, 1115, 1285, 1487, 1692, 3029, 4320, 4605 |
| HindIII | AAGCTT | 4 cut(s) | 200, 1431, 2052, 3384 |
| HpaI | GTTAAC | 3 cut(s) | 1285, 4320, 4605 |
| HpaII | CCGG | 4 cut(s) | 1232, 2017, 2443, 3446 |
| Hpy99I | CGWCG | 2 cut(s) | 3473, 4302 |
| HpyCH4IV | ACGT | 4 cut(s) | 2821, 3244, 4472, 4584 |
| HpySE526I | ACGT | 4 cut(s) | 2821, 3244, 4472, 4584 |
| Hsp92I | GRCGYC | 1 cut(s) | 4278 |
| HspAI | GCGC | 3 cut(s) | 1108, 2435, 4278 |
| KasI | GGCGCC | 1 cut(s) | 4277 |
| KpnI | GGTACC | 1 cut(s) | 2596 |
| KroI | GCCGGC | 1 cut(s) | 2016 |
| KroNI | GCCGGC | 1 cut(s) | 2018 |
| KspAI | GTTAAC | 3 cut(s) | 1285, 4320, 4605 |
| LmnI | GCTCC | 6 cut(s) | 53, 683, 1789, 2785, 3290, 3931 |
| Lsp1109I | GCAGC | 7 cut(s) | 1254, 1862, 1897, 2915, 3416, 3629, 4697 |
| MaeI | CTAG | 8 cut(s) | 767, 2012, 2169, 2292, 2400, 2864, 3159, 4418 |
| MaeII | ACGT | 4 cut(s) | 2821, 3244, 4472, 4584 |
| MfeI | CAATTG | 2 cut(s) | 2354, 2831 |
| MflI | RGATCY | 5 cut(s) | 121, 2792, 2845, 4017, 4640 |
| MhlI | GDGCHC | 3 cut(s) | 622, 2000, 2350 |
| MlsI | TGGCCA | 2 cut(s) | 1631, 3806 |
| MluNI | TGGCCA | 2 cut(s) | 1631, 3806 |
| Mly113I | GGCGCC | 1 cut(s) | 4278 |
| MlyI | GAGTC | 3 cut(s) | 1237, 1960, 4217 |
| MmeI | TCCRAC | 4 cut(s) | 1057, 1621, 1912, 1918 |
| Mox20I | TGGCCA | 2 cut(s) | 1631, 3806 |
| Mph1103I | ATGCAT | 4 cut(s) | 553, 1460, 2082, 4598 |
| MroNI | GCCGGC | 1 cut(s) | 2016 |
| MroXI | GAANNNNTTC | 5 cut(s) | 345, 1020, 1653, 4584, 4596 |
| MscI | TGGCCA | 2 cut(s) | 1631, 3806 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1824, 4488, 4553 |
| Msp20I | TGGCCA | 2 cut(s) | 1631, 3806 |
| MspA1I | CMGCKG | 1 cut(s) | 3407 |
| MspCI | CTTAAG | 2 cut(s) | 2450, 2768 |
| MspI | CCGG | 4 cut(s) | 1232, 2017, 2443, 3446 |
| MunI | CAATTG | 2 cut(s) | 2354, 2831 |
| Mva1269I | GAATGC | 5 cut(s) | 414, 459, 1614, 4596, 4598 |
| MvnI | CGCG | 1 cut(s) | 1110 |
| NaeI | GCCGGC | 1 cut(s) | 2018 |
| NarI | GGCGCC | 1 cut(s) | 4278 |
| NciI | CCSGG | 3 cut(s) | 2443, 2444, 3446 |
| NcoI | CCATGG | 1 cut(s) | 4212 |
| NdeI | CATATG | 1 cut(s) | 4270 |
| NgoMIV | GCCGGC | 1 cut(s) | 2016 |
| NmeAIII | GCCGAG | 1 cut(s) | 2329 |
| NmuCI | GTSAC | 7 cut(s) | 98, 233, 2336, 2888, 2962, 3821, 3968 |
| NsiI | ATGCAT | 4 cut(s) | 553, 1460, 2082, 4598 |
| NspI | RCATGY | 4 cut(s) | 3351, 3372, 3404, 4004 |
| OliI | CACNNNNGTG | 1 cut(s) | 4488 |
| PaeI | GCATGC | 3 cut(s) | 3372, 3404, 4004 |
| PagI | TCATGA | 2 cut(s) | 3424, 4399 |
| PaqCI | CACCTGC | 1 cut(s) | 1859 |
| PceI | AGGCCT | 6 cut(s) | 438, 462, 500, 1813, 2167, 2290 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2041 |
| PctI | GAATGC | 5 cut(s) | 414, 459, 1614, 4596, 4598 |
| PdiI | GCCGGC | 1 cut(s) | 2018 |
| PdmI | GAANNNNTTC | 5 cut(s) | 345, 1020, 1653, 4584, 4596 |
| PfoI | TCCNGGA | 1 cut(s) | 1132 |
| PkrI | GCNGC | 7 cut(s) | 1244, 1852, 1912, 2930, 3406, 3619, 4687 |
| PleI | GAGTC | 3 cut(s) | 1236, 1959, 4216 |
| PluTI | GGCGCC | 1 cut(s) | 4281 |
| PpsI | GAGTC | 3 cut(s) | 1236, 1959, 4216 |
| Ppu21I | YACGTR | 1 cut(s) | 2822 |
| PpuMI | RGGWCCY | 3 cut(s) | 2446, 3292, 3896 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3894 |
| PshBI | ATTAAT | 1 cut(s) | 3666 |
| PsiI | TTATAA | 1 cut(s) | 4254 |
| Psp124BI | GAGCTC | 1 cut(s) | 2000 |
| Psp1406I | AACGTT | 1 cut(s) | 4584 |
| Psp5II | RGGWCCY | 3 cut(s) | 2446, 3292, 3896 |
| PspFI | CCCAGC | 2 cut(s) | 2693, 3555 |
| PspPI | GGNCC | 7 cut(s) | 866, 1193, 2446, 3292, 3896, 4158, 4558 |
| PspPPI | RGGWCCY | 3 cut(s) | 2446, 3292, 3896 |
| PstI | CTGCAG | 3 cut(s) | 1095, 1852, 2660 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2928 |
| PsuI | RGATCY | 5 cut(s) | 121, 2792, 2845, 4017, 4640 |
| PvuII | CAGCTG | 1 cut(s) | 3407 |
| RigI | GGCCGGCC | 1 cut(s) | 2020 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1824, 4488, 4553 |
| SacI | GAGCTC | 1 cut(s) | 2000 |
| SalI | GTCGAC | 5 cut(s) | 771, 885, 1113, 1485, 1690 |
| SatI | GCNGC | 7 cut(s) | 1243, 1851, 1911, 2929, 3405, 3618, 4686 |
| Sau96I | GGNCC | 7 cut(s) | 866, 1193, 2446, 3292, 3896, 4158, 4558 |
| ScaI | AGTACT | 2 cut(s) | 2073, 3603 |
| SchI | GAGTC | 3 cut(s) | 1237, 1960, 4217 |
| SduI | GDGCHC | 3 cut(s) | 622, 2000, 2350 |
| SfcI | CTRYAG | 4 cut(s) | 1091, 1848, 2429, 2656 |
| SfoI | GGCGCC | 1 cut(s) | 4279 |
| SinI | GGWCC | 5 cut(s) | 1193, 2446, 3292, 3896, 4158 |
| SmaI | CCCGGG | 1 cut(s) | 2444 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1824, 4488, 4553 |
| SmlI | CTYRAG | 5 cut(s) | 621, 2450, 2669, 2768, 3333 |
| SmoI | CTYRAG | 5 cut(s) | 621, 2450, 2669, 2768, 3333 |
| SphI | GCATGC | 3 cut(s) | 3372, 3404, 4004 |
| SseBI | AGGCCT | 6 cut(s) | 438, 462, 500, 1813, 2167, 2290 |
| SsiI | CCGC | 6 cut(s) | 89, 153, 165, 444, 1110, 3983 |
| SspDI | GGCGCC | 1 cut(s) | 4277 |
| SspI | AATATT | 3 cut(s) | 679, 2719, 3670 |
| SspMI | CTAG | 8 cut(s) | 767, 2012, 2169, 2292, 2400, 2864, 3159, 4418 |
| SstI | GAGCTC | 1 cut(s) | 2000 |
| StuI | AGGCCT | 6 cut(s) | 438, 462, 500, 1813, 2167, 2290 |
| StyI | CCWWGG | 6 cut(s) | 439, 3782, 4153, 4212, 4466, 4486 |
| TaiI | ACGT | 4 cut(s) | 2824, 3247, 4475, 4587 |
| TaqI | TCGA | 9 cut(s) | 629, 772, 886, 1114, 1486, 1691, 1906, 2878, 4563 |
| TaqII | GACCGA | 2 cut(s) | 4175, 4721 |
| TatI | WGTACW | 6 cut(s) | 2071, 2827, 2883, 3454, 3529, 3601 |
| TscAI | CASTG | 8 cut(s) | 727, 2218, 2341, 2513, 3013, 3304, 3500, 4683 |
| TseFI | GTSAC | 7 cut(s) | 98, 233, 2336, 2888, 2962, 3821, 3968 |
| TseI | GCWGC | 7 cut(s) | 1242, 1850, 1910, 2928, 3404, 3617, 4685 |
| Tsp45I | GTSAC | 7 cut(s) | 98, 233, 2336, 2888, 2962, 3821, 3968 |
| TspGWI | ACGGA | 5 cut(s) | 1313, 2210, 2333, 2530, 4286 |
| TspMI | CCCGGG | 1 cut(s) | 2442 |
| TspRI | CASTG | 8 cut(s) | 727, 2218, 2341, 2513, 3013, 3304, 3500, 4683 |
| Vha464I | CTTAAG | 2 cut(s) | 2450, 2768 |
| VpaK11BI | GGWCC | 5 cut(s) | 1193, 2446, 3292, 3896, 4158 |
| VspI | ATTAAT | 1 cut(s) | 3666 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 2453, 2672, 4490 |
| XapI | RAATTY | 8 cut(s) | 105, 345, 368, 2216, 2261, 3797, 4144, 4349 |
| XbaI | TCTAGA | 1 cut(s) | 4417 |
| XceI | RCATGY | 4 cut(s) | 3351, 3372, 3404, 4004 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3570 |
| XmaI | CCCGGG | 1 cut(s) | 2442 |
| XmiI | GTMKAC | 6 cut(s) | 772, 886, 1114, 1486, 1691, 2818 |
| XmnI | GAANNNNTTC | 5 cut(s) | 345, 1020, 1653, 4584, 4596 |
| XspI | CTAG | 8 cut(s) | 767, 2012, 2169, 2292, 2400, 2864, 3159, 4418 |
| ZrmI | AGTACT | 2 cut(s) | 2073, 3603 |
| Zsp2I | ATGCAT | 4 cut(s) | 553, 1460, 2082, 4598 |