Rorug01G0474500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008103
Physical Location & Seq
Reverse (-)
56491025 .. 56497000
5976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0474500.1

Sequence Viewer

Length: 1410 bp
ATGTTTGAGGGCAAAGCTTTGAAGAGAAACCGAGGCACTACTGAGCCCATCAACACCAAGCCTTTGATTGGCTATGCAGTTGTAGGCCCCATTGGGAATGTTTCTAACACTGTTGCTTCAACCCTGGAACGTGCTGATAGAATCCAACCTGCTGTTTCTGTCAGTAGTCTGGGTAATATGGAGAACAGCGGGCTTGTTGATGTTAAACCAGGGATCCCTGATGTTATAGACAAGATTAAGAGATGCAAAGTTTCTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCCGACTCTAAAACAGCTGGAAAGGTTGTGAGACTAATGTACACCAATGACGGCTTGGCTCTGTTAGCGCTGGCCTCCAATGCTGTTCACAAGATTTGGAAATGGCCGCGTGATGACAGGAATAATCCATCAGGAAAGGCTAGTGCATTTGTTGTTCCACAGTTGTGGCAGCCTCGGAATGGAATTCTTATGGCTAATGATGTAAATGACAATAAGCCTGCTGAGGAATATACTGCATGCATTGCTGTATATAAAAATGATTCTTACATGATGTCTGCATCTGGCGGTAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGAAAACATTTGTGTCTCCTCCTCCTGCTGCCACTTTTCTGGCATTCCATCCTCAAAATAATAATATAATTGCTATTGGCATGGAGGACTCTACTATTTTAATATATAATATCAGGGTTGATGAGGTCGAAACCAAACTGAAGGGTCACCGGAATCGGATAATGGGCCTTGCGTTTTCCCAAACTCTTAACATACTGGTTTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTTTTGGGTGGGAGAAAAAGAAAACAACGTTAATACAAGCACCAACTGGTCGGCAATCCCCTTTGGTTGGAGAAACAAAAATTCAGTTTCATAACGATCACACACATCTATTGGTTGCCCATGAAAGCCAAATTGCTGTGTATGACAGCAAGCTTGATTGTTTACGCTCTTGGACTCCCAAAGATGCACTTGCTGCTCCCATCTCATGTGCAATATATTCATGCGATGGTTTGGTTGTTTATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTTGATGCTAATAGCTTAAGACTCAGATGTCGAATAGTGCCTTCTGCTTATATACCATCATTTTCTCTCAGCGGTGGCAACCCCTCCTATCCTCTGGTTGTAGCAGCTCATCCATCTGAGCCTAACCAGATTGCAGTTGGCATGACTGACGGGTCAGTCCATGTGGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGCACACCCTCTCAAGATATTGGTCCTTCCAATTCATCAAATCATTCTCTTAGTAGTCAAGCATCAGAACTTCCTTCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

469

Amino Acids

50.74

Weight (kDa)

8.5

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 166 - 287 1.5e-13 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 169 - 381 2.9e-19 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 174 - 286 1.9e-15 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 175 - 289 1.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 178 - 294 1.7e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 180 - 416 2.6e-15 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 187 - 286 8.1e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 189 - 286 1.2e-10 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 190 - 286 2e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 195 - 288 1e-08 WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st PF23770 203 - 268 7.1e-07 RIG first beta-propeller
WD40_Prp19 PF24814 214 - 381 6.2e-14 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1155, 1279, 1283
Acc36I ACCTGC 1 cut(s) 157
AccII CGCG 1 cut(s) 403
AciI CCGC 5 cut(s) 189, 263, 401, 579, 1200
AclI AACGTT 1 cut(s) 884
AclWI GGATC 4 cut(s) 208, 221, 260, 273
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 2 cut(s) 477, 936
AcuI CTGAAG 3 cut(s) 779, 807, 1337
AfaI GTAC 1 cut(s) 335
AfeI AGCGCT 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 68, 473, 923
AflII CTTAAG 1 cut(s) 1144
AgsI TTSAA 4 cut(s) 22, 120, 598, 1298
AjnI CCWGG 3 cut(s) 123, 208, 1403
AleI CACNNNNGTG 1 cut(s) 457
AluBI AGCT 8 cut(s) 17, 284, 311, 830, 1009, 1143, 1235, 1315
AluI AGCT 8 cut(s) 17, 284, 311, 830, 1009, 1143, 1235, 1315
Alw26I GTCTC 3 cut(s) 319, 592, 639
AlwI GGATC 4 cut(s) 208, 221, 260, 273
AlwNI CAGNNNCTG 1 cut(s) 830
Aor51HI AGCGCT 1 cut(s) 363
AoxI GGCC 4 cut(s) 85, 366, 398, 784
ApeKI GCWGC 4 cut(s) 463, 647, 1049, 1232
ApoI RAATTY 2 cut(s) 477, 936
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 3 cut(s) 86, 784, 1349
AsuHPI GGTGA 1 cut(s) 758
AvaII GGWCC 1 cut(s) 1349
BamHI GGATCC 2 cut(s) 213, 265
BanII GRGCYC 1 cut(s) 48
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 4 cut(s) 475, 634, 1036, 1244
BccI CCATC 8 cut(s) 56, 430, 675, 1064, 1076, 1192, 1249, 1309
BceAI ACGGC 1 cut(s) 361
BciT130I CCWGG 3 cut(s) 125, 210, 1405
BcoDI GTCTC 3 cut(s) 319, 592, 639
BfaI CTAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 365
BfrI CTTAAG 1 cut(s) 1144
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 5 cut(s) 401, 464, 648, 1050, 1233
BlsI GCNGC 5 cut(s) 402, 465, 649, 1051, 1234
Bme1390I CCNGG 3 cut(s) 125, 210, 1405
Bme18I GGWCC 1 cut(s) 1349
BmgT120I GGNCC 3 cut(s) 86, 784, 1349
BmiI GGNNCC 3 cut(s) 88, 215, 267
BmrFI CCNGG 3 cut(s) 125, 210, 1405
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 7 cut(s) 233, 581, 823, 1030, 1123, 1297, 1397
BmuI ACTGGG 1 cut(s) 832
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 1323
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 4 cut(s) 31, 123, 209, 467
BsaWI WCCGGW 1 cut(s) 768
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 473, 923
Bse1I ACTGG 3 cut(s) 819, 838, 907
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 3 cut(s) 125, 210, 1405
BseDI CCNNGG 4 cut(s) 31, 123, 209, 467
BseGI GGATG 2 cut(s) 667, 1237
BseLI CCNNNNNNNGG 3 cut(s) 68, 473, 923
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 5 cut(s) 33, 507, 1165, 1210, 1236
BseNI ACTGG 3 cut(s) 819, 838, 907
BseRI GAGGAG 2 cut(s) 627, 630
BseXI GCAGC 4 cut(s) 475, 634, 1036, 1244
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 4 cut(s) 87, 368, 400, 786
BsiSI CCGG 1 cut(s) 769
BslI CCNNNNNNNGG 3 cut(s) 68, 473, 923
BsmAI GTCTC 3 cut(s) 319, 592, 639
BsmI GAATGC 1 cut(s) 662
BsnI GGCC 4 cut(s) 87, 368, 400, 786
Bso31I GGTCTC 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 48
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 3 cut(s) 213, 265, 952
BspACI CCGC 5 cut(s) 189, 263, 401, 579, 1200
BspANI GGCC 4 cut(s) 87, 368, 400, 786
BspCNI CTCAG 5 cut(s) 34, 508, 1164, 1209, 1237
BspFNI CGCG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 88, 215, 267
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 4 cut(s) 208, 221, 260, 273
BspTI CTTAAG 1 cut(s) 1144
BspTNI GGTCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 3 cut(s) 819, 838, 907
BssECI CCNNGG 4 cut(s) 31, 123, 209, 467
BssMI GATC 3 cut(s) 213, 265, 952
Bst2UI CCWGG 3 cut(s) 125, 210, 1405
Bst4CI ACNGT 3 cut(s) 112, 456, 1115
Bst6I CTCTTC 1 cut(s) 17
BstAFI CTTAAG 1 cut(s) 1144
BstAPI GCANNNNNTGC 2 cut(s) 536, 1049
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 5 cut(s) 191, 366, 513, 532, 1007
BstDEI CTNAG 6 cut(s) 42, 516, 1151, 1196, 1245, 1376
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 2 cut(s) 667, 1237
BstFNI CGCG 1 cut(s) 403
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 3 cut(s) 216, 268, 955
BstMAI GTCTC 3 cut(s) 319, 592, 639
BstMBI GATC 3 cut(s) 213, 265, 952
BstMWI GCNNNNNNNGC 4 cut(s) 359, 374, 536, 1049
BstNI CCWGG 3 cut(s) 125, 210, 1405
BstNSI RCATGY 1 cut(s) 534
BstPI GGTNACC 1 cut(s) 764
BstSCI CCNGG 3 cut(s) 123, 208, 1403
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 4 cut(s) 475, 634, 1036, 1244
BstX2I RGATCY 2 cut(s) 213, 265
BstXI CCANNNNNNTGG 2 cut(s) 459, 658
BstYI RGATCY 2 cut(s) 213, 265
BsuRI GGCC 4 cut(s) 87, 368, 400, 786
BtgZI GCGATG 1 cut(s) 1095
BtsCI GGATG 2 cut(s) 667, 1237
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 5 cut(s) 191, 366, 513, 532, 1007
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 1 cut(s) 618
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 86, 784, 1349
Csp6I GTAC 1 cut(s) 334
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 6 cut(s) 42, 516, 1151, 1196, 1245, 1376
DpnI GATC 3 cut(s) 215, 267, 954
DpnII GATC 3 cut(s) 213, 265, 952
DrdI GACNNNNNNGTC 3 cut(s) 1155, 1279, 1283
DseDI GACNNNNNNGTC 3 cut(s) 1155, 1279, 1283
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 1349
Eco47III AGCGCT 1 cut(s) 363
Eco57I CTGAAG 3 cut(s) 779, 807, 1337
Eco91I GGTNACC 1 cut(s) 764
EcoO109I RGGNCCY 1 cut(s) 86
EcoO65I GGTNACC 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 3 cut(s) 123, 208, 1403
EcoT22I ATGCAT 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 48
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 5 cut(s) 401, 464, 648, 1050, 1233
FokI GGATG 2 cut(s) 654, 1224
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 5 cut(s) 401, 464, 648, 1050, 1233
FspBI CTAG 1 cut(s) 435
GlaI GCGC 1 cut(s) 363
GluI GCNGC 5 cut(s) 401, 464, 648, 1050, 1233
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 4 cut(s) 87, 368, 400, 786
HapII CCGG 1 cut(s) 769
HhaI GCGC 1 cut(s) 364
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HindIII AAGCTT 3 cut(s) 15, 282, 1007
HinfI GANTC 7 cut(s) 141, 299, 554, 707, 772, 1030, 1149
HpaII CCGG 1 cut(s) 769
HphI GGTGA 1 cut(s) 758
Hpy166II GTNNAC 3 cut(s) 336, 382, 1019
Hpy188I TCNGA 7 cut(s) 256, 471, 777, 1154, 1246, 1306, 1393
Hpy188III TCNNGA 4 cut(s) 426, 619, 825, 1340
Hpy8I GTNNAC 3 cut(s) 336, 382, 1019
HpyAV CCTTC 5 cut(s) 279, 754, 1179, 1362, 1410
HpyCH4III ACNGT 3 cut(s) 112, 456, 1115
HpyCH4IV ACGT 2 cut(s) 130, 884
HpyF10VI GCNNNNNNNGC 4 cut(s) 359, 374, 536, 1049
HpyF3I CTNAG 6 cut(s) 42, 516, 1151, 1196, 1245, 1376
HpySE526I ACGT 2 cut(s) 130, 884
HspAI GCGC 1 cut(s) 362
Kzo9I GATC 3 cut(s) 213, 265, 952
LmnI GCTCC 3 cut(s) 827, 852, 1057
Lsp1109I GCAGC 4 cut(s) 475, 634, 1036, 1244
LweI GCATC 7 cut(s) 233, 581, 823, 1030, 1123, 1297, 1397
MaeI CTAG 1 cut(s) 435
MaeII ACGT 2 cut(s) 130, 884
MaeIII GTNAC 2 cut(s) 764, 1109
MalI GATC 3 cut(s) 215, 267, 954
MboI GATC 3 cut(s) 213, 265, 952
MboII GAAGA 2 cut(s) 34, 813
MflI RGATCY 2 cut(s) 213, 265
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 5 cut(s) 477, 687, 936, 987, 1357
MlyI GAGTC 4 cut(s) 293, 701, 1024, 1143
MmeI TCCRAC 2 cut(s) 169, 904
Mph1103I ATGCAT 1 cut(s) 536
MseI TTAA 8 cut(s) 204, 237, 287, 612, 719, 807, 887, 1145
MslI CAYNNNNRTG 1 cut(s) 457
MspA1I CMGCKG 3 cut(s) 189, 311, 1200
MspCI CTTAAG 1 cut(s) 1144
MspI CCGG 1 cut(s) 769
MspR9I CCNGG 3 cut(s) 125, 210, 1405
Mva1269I GAATGC 1 cut(s) 662
MvaI CCWGG 3 cut(s) 125, 210, 1405
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 4 cut(s) 359, 374, 536, 1049
NdeII GATC 3 cut(s) 213, 265, 952
NlaIV GGNNCC 3 cut(s) 88, 215, 267
NmuCI GTSAC 2 cut(s) 764, 1109
NsiI ATGCAT 1 cut(s) 536
NspI RCATGY 1 cut(s) 534
OliI CACNNNNGTG 1 cut(s) 457
PaeI GCATGC 1 cut(s) 534
PagI TCATGA 1 cut(s) 618
PctI GAATGC 1 cut(s) 662
PfeI GAWTC 3 cut(s) 141, 554, 772
PkrI GCNGC 5 cut(s) 402, 465, 649, 1051, 1234
PleI GAGTC 4 cut(s) 293, 701, 1024, 1143
PpsI GAGTC 4 cut(s) 293, 701, 1024, 1143
Psp1406I AACGTT 1 cut(s) 884
Psp6I CCWGG 3 cut(s) 123, 208, 1403
PspEI GGTNACC 1 cut(s) 764
PspGI CCWGG 3 cut(s) 123, 208, 1403
PspN4I GGNNCC 3 cut(s) 88, 215, 267
PspPI GGNCC 3 cut(s) 86, 784, 1349
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 213, 265
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 457
SaqAI TTAA 8 cut(s) 204, 237, 287, 612, 719, 807, 887, 1145
SatI GCNGC 5 cut(s) 401, 464, 648, 1050, 1233
Sau3AI GATC 3 cut(s) 213, 265, 952
Sau96I GGNCC 3 cut(s) 86, 784, 1349
SchI GAGTC 4 cut(s) 293, 701, 1024, 1143
ScrFI CCNGG 3 cut(s) 125, 210, 1405
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 7 cut(s) 233, 581, 823, 1030, 1123, 1297, 1397
SinI GGWCC 1 cut(s) 1349
SmiMI CAYNNNNRTG 1 cut(s) 457
SmlI CTYRAG 2 cut(s) 1144, 1338
SmoI CTYRAG 2 cut(s) 1144, 1338
SphI GCATGC 1 cut(s) 534
Sse9I AATT 5 cut(s) 477, 687, 936, 987, 1357
SsiI CCGC 5 cut(s) 189, 263, 401, 579, 1200
SspMI CTAG 1 cut(s) 435
StyD4I CCNGG 3 cut(s) 123, 208, 1403
TaaI ACNGT 3 cut(s) 112, 456, 1115
TaiI ACGT 2 cut(s) 133, 887
TaqI TCGA 2 cut(s) 747, 1159
TasI AATT 5 cut(s) 477, 687, 936, 987, 1357
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 3 cut(s) 141, 554, 772
Tru1I TTAA 8 cut(s) 204, 237, 287, 612, 719, 807, 887, 1145
Tru9I TTAA 8 cut(s) 204, 237, 287, 612, 719, 807, 887, 1145
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 764, 1109
TseI GCWGC 4 cut(s) 463, 647, 1049, 1232
Tsp45I GTSAC 2 cut(s) 764, 1109
TspDTI ATGAA 6 cut(s) 293, 635, 935, 993, 1065, 1350
TspRI CASTG 1 cut(s) 115
Vha464I CTTAAG 1 cut(s) 1144
VpaK11BI GGWCC 1 cut(s) 1349
XapI RAATTY 2 cut(s) 477, 936
XceI RCATGY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 2 cut(s) 346, 1262
XspI CTAG 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.