Rh7CG256500

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008103
Physical Location & Seq
Forward (+)
24901283 .. 24907183
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG256500.1

Sequence Viewer

Length: 1338 bp
ATGCAGAATCCTTCAGCTGAACTTGAAGACATTCTGAGGAACAGTATAGACGCGTATCCAAATCCTCCGGTATCGGCGCCACACTGGATCTATCTCTCTTCCAATCCTCCACCATATCTTGATGCGACTCTAGAAAACCTTCAATACATTAAACACCAACAATTCGTAGGGATAGATGACTTTGCAACGATACAAATATTCAAAAATCGTGATGATGCTTATAGACATTGGAGGATTCAGAAGAGCCTCGACTCGAAATTTTTCATACAGCCACAACGATGTGTACAATTTCAAGGACCGACATCAAAAACCTTATATTATGTGCTTATTTTCGACAAAATTATTAGAGAATTCAGAGTCTGGGTTAAAGCACATGCTAACAGTCAAGTCTCCGATAAGGTGTTGTGGGAGCTCTTGAAGAAGATTGGAAGAAACATACGATATTTGTCTACAGAGATACAAGCACCTCGACCACATAACAATCTTCTTCAAGGTATAGGTATAACAGCAGACCTTCAAGTTTTCTTCTTCAACTTGGGTAGTGCTGCTACAAACACGTGCCTTAAGGACGTTCAAGAGTTCGATGGAATTCTTACTTCAATCGAACCGAACTTGGTGGACTTGGAGATAAAAGAGCGTGTTAATAGGTGGAGGTCATTTTTCTCCACGAGTTGTTTAGCACCTAATCCAACTCCAATCAAGGGTTCACATCTGTATAATCCCCTTAATCTTGACCATGTTAAATTGATCTTCAACTATCCGAGTGTCTGGAACGATGAAGACAATCTCCGATTTCTGGCAAATATGCATAAACTATGCACGGATATATGCCGTAAGCATCGGCATTCTAATTTGTTAGATAATCAGGAATTTAGAAATACATTCAATATTCCCTATTCTCAATGGGACAGCCGAGTGACGGCCACAACTAGTGCCTTAAATGAAGTGTACCAATTTGGTATTGCCAAAGGAAATTACAAAAGGTTTGACCGAATTACCTGTACTCGGGTACTATCGGGGGCATCCATTGTTACTTTTGCCCACAATTGTGTCAAACACTTTTGTGAGAGGGCGCAAAACAATAACAATCATGGTGGTATACAAATGATCAATCTCTCTTTTCTCCGATCCAAATCTCAAACCCAAATCAATAACAACTCAGCCCTCACCGTCTCAACCATTTTTGACTCCGACAAGGCTTCCTCTCGCTTCCAACGCCTTTGCTCCGTTTCCAATTCGCCACTCTCCCTGTCCGACGATCTACACGTCAAGCCGTCTATGCTCTTTGTAACCACTCTCACGGAGTCCGTTTCGTGTTCCGGCGTTGGTGTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

445

Amino Acids

50.98

Weight (kDa)

8.81

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccI GTMKAC 2 cut(s) 449, 1099
AccII CGCG 1 cut(s) 53
AclWI GGATC 2 cut(s) 95, 1122
AcoI YGGCCR 1 cut(s) 921
AcsI RAATTY 4 cut(s) 257, 350, 588, 869
AcvI CACGTG 1 cut(s) 558
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 285, 950, 1003, 1011
AfiI CCNNNNNNNGG 4 cut(s) 701, 796, 919, 1005
AflII CTTAAG 1 cut(s) 563
AflIII ACRYGT 3 cut(s) 51, 555, 1264
AhlI ACTAGT 1 cut(s) 929
AjiI CACGTC 1 cut(s) 1267
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AleI CACNNNNGTG 2 cut(s) 1047, 1062
AluBI AGCT 2 cut(s) 17, 412
AluI AGCT 2 cut(s) 17, 412
Alw21I GWGCWC 2 cut(s) 414, 1335
Alw26I GTCTC 2 cut(s) 394, 1177
Alw44I GTGCAC 1 cut(s) 1331
AlwI GGATC 2 cut(s) 95, 1122
AlwNI CAGNNNCTG 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 1005
AoxI GGCC 1 cut(s) 921
ApaLI GTGCAC 1 cut(s) 1331
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 4 cut(s) 257, 350, 588, 869
Asp700I GAANNNNTTC 3 cut(s) 30, 138, 260
AspLEI GCGC 2 cut(s) 79, 1075
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 1159
AvaI CYCGRG 1 cut(s) 1005
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 1335
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 1 cut(s) 667
BbrPI CACGTG 1 cut(s) 558
BbsI GAAGAC 2 cut(s) 33, 786
Bbv12I GWGCWC 2 cut(s) 414, 1335
BbvI GCAGC 1 cut(s) 532
BccI CCATC 1 cut(s) 578
BceAI ACGGC 3 cut(s) 816, 936, 1258
BciVI GTATCC 1 cut(s) 66
BclI TGATCA 1 cut(s) 1107
BcoDI GTCTC 2 cut(s) 394, 1177
BcuI ACTAGT 1 cut(s) 929
BfaI CTAG 2 cut(s) 131, 930
BfmI CTRYAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 80
BfrI CTTAAG 1 cut(s) 563
BfuI GTATCC 1 cut(s) 66
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 1005
BmgBI CACGTC 1 cut(s) 1267
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 78
BmsI GCATC 4 cut(s) 112, 205, 847, 1031
BpiI GAAGAC 2 cut(s) 33, 786
BsaAI YACGTR 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 1132
BsaHI GRCGYC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 4 cut(s) 701, 796, 919, 1005
Bse1I ACTGG 1 cut(s) 89
Bse8I GATNNNNATC 1 cut(s) 1132
BseGI GGATG 1 cut(s) 1022
BseJI GATNNNNATC 1 cut(s) 1132
BseLI CCNNNNNNNGG 4 cut(s) 701, 796, 919, 1005
BseMII CTCAG 2 cut(s) 26, 1173
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 1335
BseXI GCAGC 1 cut(s) 532
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 923
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 414, 1335
BsiHKCI CYCGRG 1 cut(s) 1005
BsiSI CCGG 2 cut(s) 68, 1320
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 4 cut(s) 701, 796, 919, 1005
BsmAI GTCTC 2 cut(s) 394, 1177
BsmBI CGTCTC 1 cut(s) 1177
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 1 cut(s) 923
BsoBI CYCGRG 1 cut(s) 1005
Bsp1286I GDGCHC 2 cut(s) 414, 1335
Bsp1407I TGTACA 1 cut(s) 283
Bsp143I GATC 5 cut(s) 87, 747, 1107, 1127, 1258
BspANI GGCC 1 cut(s) 923
BspCNI CTCAG 2 cut(s) 27, 1172
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 2 cut(s) 95, 1122
BspQI GCTCTTC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 563
BsrGI TGTACA 1 cut(s) 283
BsrI ACTGG 1 cut(s) 89
BssMI GATC 5 cut(s) 87, 747, 1107, 1127, 1258
BssNAI GTATAC 1 cut(s) 1100
BssNI GRCGYC 1 cut(s) 77
BssSI CACGAG 1 cut(s) 667
Bst1107I GTATAC 1 cut(s) 1100
Bst2BI CACGAG 1 cut(s) 667
Bst4CI ACNGT 3 cut(s) 44, 383, 1171
Bst6I CTCTTC 2 cut(s) 103, 236
BstACI GRCGYC 1 cut(s) 77
BstAFI CTTAAG 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 283
BstBAI YACGTR 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 35, 1159
BstF5I GGATG 1 cut(s) 1022
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 79, 1075
BstKTI GATC 5 cut(s) 90, 750, 1110, 1130, 1261
BstMAI GTCTC 2 cut(s) 394, 1177
BstMBI GATC 5 cut(s) 87, 747, 1107, 1127, 1258
BstMWI GCNNNNNNNGC 2 cut(s) 1215, 1279
BstNSI RCATGY 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 1335
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 532
BstV2I GAAGAC 2 cut(s) 33, 786
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BstZ17I GTATAC 1 cut(s) 1100
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 923
BtrI CACGTC 1 cut(s) 1267
BtsCI GGATG 1 cut(s) 1022
BtsIMutI CAGTG 2 cut(s) 82, 1333
CaiI CAGNNNCTG 1 cut(s) 360
CfoI GCGC 2 cut(s) 79, 1075
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 59
Csp6I GTAC 4 cut(s) 284, 949, 1002, 1010
CspCI CAANNNNNGTGG 2 cut(s) 1075, 1110
CviAII CATG 3 cut(s) 374, 737, 1091
CviJI RGCY 9 cut(s) 17, 246, 271, 412, 912, 923, 1163, 1199, 1273
CviKI_1 RGCY 9 cut(s) 17, 246, 271, 412, 912, 923, 1163, 1199, 1273
CviQI GTAC 4 cut(s) 284, 949, 1002, 1010
DdeI CTNAG 2 cut(s) 35, 1159
DinI GGCGCC 1 cut(s) 78
DpnI GATC 5 cut(s) 89, 749, 1109, 1129, 1260
DpnII GATC 5 cut(s) 87, 747, 1107, 1127, 1258
EaeI YGGCCR 1 cut(s) 921
Eam1104I CTCTTC 2 cut(s) 103, 236
EarI CTCTTC 2 cut(s) 103, 236
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 412
Eco72I CACGTG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 1005
EcoICRI GAGCTC 1 cut(s) 412
EcoRI GAATTC 2 cut(s) 350, 588
EcoT22I ATGCAT 1 cut(s) 810
EcoT38I GRGCYC 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 1177
FaeI CATG 3 cut(s) 377, 740, 1094
FaqI GGGAC 1 cut(s) 920
FatI CATG 3 cut(s) 373, 736, 1090
FbaI TGATCA 1 cut(s) 1107
FblI GTMKAC 2 cut(s) 449, 1099
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 1 cut(s) 1009
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 2 cut(s) 131, 930
GlaI GCGC 2 cut(s) 78, 1074
GluI GCNGC 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 1 cut(s) 923
HapII CCGG 2 cut(s) 68, 1320
HgaI GACGC 1 cut(s) 59
HhaI GCGC 2 cut(s) 79, 1075
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 3 cut(s) 377, 740, 1094
Hin6I GCGC 2 cut(s) 77, 1073
HinP1I GCGC 2 cut(s) 77, 1073
HinfI GANTC 7 cut(s) 7, 127, 235, 251, 357, 1187, 1304
HpaII CCGG 2 cut(s) 68, 1320
HphI GGTGA 1 cut(s) 1159
Hpy166II GTNNAC 7 cut(s) 284, 450, 619, 707, 949, 1100, 1333
Hpy188I TCNGA 9 cut(s) 36, 240, 356, 394, 762, 791, 1127, 1192, 1255
Hpy188III TCNNGA 8 cut(s) 119, 131, 209, 415, 575, 731, 769, 866
Hpy8I GTNNAC 7 cut(s) 284, 450, 619, 707, 949, 1100, 1333
Hpy99I CGWCG 1 cut(s) 1259
HpyAV CCTTC 3 cut(s) 21, 149, 524
HpyCH4III ACNGT 3 cut(s) 44, 383, 1171
HpyCH4IV ACGT 3 cut(s) 557, 570, 1266
HpyCH4V TGCA 5 cut(s) 4, 185, 808, 819, 1333
HpyF10VI GCNNNNNNNGC 2 cut(s) 1215, 1279
HpyF3I CTNAG 2 cut(s) 35, 1159
HpySE526I ACGT 3 cut(s) 557, 570, 1266
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 3 cut(s) 377, 740, 1094
HspAI GCGC 2 cut(s) 77, 1073
KasI GGCGCC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 1107
Kzo9I GATC 5 cut(s) 87, 747, 1107, 1127, 1258
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 2 cut(s) 409, 1229
LpnPI CCDG 9 cut(s) 70, 81, 346, 754, 782, 851, 1012, 1262, 1333
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 4 cut(s) 112, 205, 847, 1031
MaeI CTAG 2 cut(s) 131, 930
MaeII ACGT 3 cut(s) 557, 570, 1266
MaeIII GTNAC 3 cut(s) 916, 1030, 1288
MalI GATC 5 cut(s) 89, 749, 1109, 1129, 1260
MboI GATC 5 cut(s) 87, 747, 1107, 1127, 1258
MfeI CAATTG 1 cut(s) 1045
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 2 cut(s) 414, 1335
MluI ACGCGT 1 cut(s) 51
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 5 cut(s) 121, 245, 366, 1181, 1313
MmeI TCCRAC 4 cut(s) 713, 1215, 1237, 1278
Mph1103I ATGCAT 1 cut(s) 810
MroXI GAANNNNTTC 3 cut(s) 30, 138, 260
MseI TTAA 7 cut(s) 150, 366, 564, 642, 726, 741, 938
MslI CAYNNNNRTG 3 cut(s) 277, 1047, 1062
MspA1I CMGCKG 1 cut(s) 17
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 2 cut(s) 68, 1320
MunI CAATTG 1 cut(s) 1045
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 1215, 1279
NarI GGCGCC 1 cut(s) 77
NdeII GATC 5 cut(s) 87, 747, 1107, 1127, 1258
NlaIII CATG 3 cut(s) 377, 740, 1094
NlaIV GGNNCC 1 cut(s) 78
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 1 cut(s) 916
NsiI ATGCAT 1 cut(s) 810
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 2 cut(s) 1047, 1062
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 1263
PctI GAATGC 1 cut(s) 844
PdmI GAANNNNTTC 3 cut(s) 30, 138, 260
PfeI GAWTC 2 cut(s) 7, 235
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 5 cut(s) 121, 245, 365, 1181, 1312
PluTI GGCGCC 1 cut(s) 80
PmaCI CACGTG 1 cut(s) 558
PmlI CACGTG 1 cut(s) 558
PpsI GAGTC 5 cut(s) 121, 245, 365, 1181, 1312
Ppu21I YACGTR 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 414
PspCI CACGTG 1 cut(s) 558
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 360
PsuI RGATCY 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 4 cut(s) 285, 950, 1003, 1011
RsaNI GTAC 4 cut(s) 284, 949, 1002, 1010
RseI CAYNNNNRTG 3 cut(s) 277, 1047, 1062
SacI GAGCTC 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 7 cut(s) 150, 366, 564, 642, 726, 741, 938
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 87, 747, 1107, 1127, 1258
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 5 cut(s) 121, 245, 366, 1181, 1313
SduI GDGCHC 2 cut(s) 414, 1335
SfaNI GCATC 4 cut(s) 112, 205, 847, 1031
SfcI CTRYAG 1 cut(s) 450
SfoI GGCGCC 1 cut(s) 78
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 3 cut(s) 277, 1047, 1062
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
SpeI ACTAGT 1 cut(s) 929
SspDI GGCGCC 1 cut(s) 76
SspI AATATT 2 cut(s) 198, 889
SspMI CTAG 2 cut(s) 131, 930
SstI GAGCTC 1 cut(s) 414
TaaI ACNGT 3 cut(s) 44, 383, 1171
TaiI ACGT 3 cut(s) 560, 573, 1269
TaqI TCGA 6 cut(s) 249, 254, 333, 469, 582, 603
TaqII GACCGA 2 cut(s) 313, 1005
TatI WGTACW 2 cut(s) 283, 1001
TfiI GAWTC 2 cut(s) 7, 235
Tru1I TTAA 7 cut(s) 150, 366, 564, 642, 726, 741, 938
Tru9I TTAA 7 cut(s) 150, 366, 564, 642, 726, 741, 938
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 916
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 916
TspDTI ATGAA 3 cut(s) 253, 792, 957
TspGWI ACGGA 4 cut(s) 836, 1216, 1297, 1316
TspRI CASTG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 563
VneI GTGCAC 1 cut(s) 1331
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 4 cut(s) 257, 350, 588, 869
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 449, 1099
XmnI GAANNNNTTC 3 cut(s) 30, 138, 260
XspI CTAG 2 cut(s) 131, 930
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.