Rorug02G0141800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002007
Physical Location & Seq
Reverse (-)
12493526 .. 12500085
6560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0141800.1

Sequence Viewer

Length: 495 bp
ATGGAAAGGTGGTGGTTTGGCAAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACCTTCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCTGGCATTATCATCAGGTACTTCAACAAGATTTTCCGTAGCATCCCAGGCAGTGAACTTAATAAGGTCAAAAATGAACATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTCCAGCACAGCTAGATCTAAATGATACAGCACCAGAGAGCAGTCATCTTAAGTTGCGTGACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGATGGGTAAAGAGGGATACGGAGGCATGTGTTGTTTGCTAGATTTCGCGGAAGTGGGGAGTTCTCTTGAACCAGCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

164

Amino Acids

18.22

Weight (kDa)

7.75

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 8 - 136 1.7e-09 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 461
AciI CCGC 2 cut(s) 72, 461
AcsI RAATTY 1 cut(s) 386
AcvI CACGTG 1 cut(s) 119
AfaI GTAC 2 cut(s) 101, 187
AflII CTTAAG 1 cut(s) 341
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 3 cut(s) 80, 192, 482
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 1 cut(s) 304
AluI AGCT 1 cut(s) 304
Alw26I GTCTC 1 cut(s) 401
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 83, 115
BbrPI CACGTG 1 cut(s) 119
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 215
BciVI GTATCC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 305, 452
BfrI CTTAAG 1 cut(s) 341
BfuI GTATCC 1 cut(s) 422
BglII AGATCT 1 cut(s) 307
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 2 cut(s) 46, 218
BpmI CTGGAG 1 cut(s) 279
BsaAI YACGTR 2 cut(s) 103, 119
BsaJI CCNNGG 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 401
BsnI GGCC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 108, 295
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 72, 461
BspANI GGCC 1 cut(s) 128
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 341
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 1 cut(s) 86
BstAFI CTTAAG 1 cut(s) 341
BstBAI YACGTR 2 cut(s) 103, 119
BstF5I GGATG 1 cut(s) 209
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 3 cut(s) 71, 170, 215
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 1 cut(s) 209
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 3 cut(s) 132, 225, 400
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 100, 186
CviAII CATG 2 cut(s) 247, 439
CviJI RGCY 4 cut(s) 65, 128, 293, 304
CviKI_1 RGCY 4 cut(s) 65, 128, 293, 304
CviQI GTAC 2 cut(s) 100, 186
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 295
Eco72I CACGTG 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 295
FaeI CATG 2 cut(s) 250, 442
FaiI YATR 4 cut(s) 68, 248, 440, 493
FatI CATG 2 cut(s) 246, 438
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 2 cut(s) 305, 452
GluI GCNGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 1 cut(s) 128
Hin1II CATG 2 cut(s) 250, 442
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 374
HpaI GTTAAC 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 223, 361
Hpy188III TCNNGA 3 cut(s) 80, 145, 479
Hpy8I GTNNAC 2 cut(s) 223, 361
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 80, 123
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 3 cut(s) 102, 118, 257
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 170, 215
HpySE526I ACGT 3 cut(s) 102, 118, 257
Hsp92II CATG 2 cut(s) 250, 442
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 307
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 9 cut(s) 110, 120, 130, 155, 168, 200, 227, 309, 339
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 46, 218
MaeI CTAG 2 cut(s) 305, 452
MaeII ACGT 3 cut(s) 102, 118, 257
MaeIII GTNAC 2 cut(s) 34, 350
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 1 cut(s) 103
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 108, 295
MluCI AATT 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 3 cut(s) 140, 418, 428
MseI TTAA 4 cut(s) 227, 342, 360, 390
MspCI CTTAAG 1 cut(s) 341
MspR9I CCNGG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 3 cut(s) 71, 170, 215
NdeII GATC 1 cut(s) 307
NlaIII CATG 2 cut(s) 250, 442
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 1 cut(s) 163
PmaCI CACGTG 1 cut(s) 119
PmlI CACGTG 1 cut(s) 119
Ppu21I YACGTR 2 cut(s) 103, 119
Psp6I CCWGG 1 cut(s) 213
PspCI CACGTG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 127
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 2 cut(s) 101, 187
RsaNI GTAC 2 cut(s) 100, 186
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 4 cut(s) 227, 342, 360, 390
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 2 cut(s) 108, 295
SetI ASST 8 cut(s) 11, 105, 115, 121, 187, 236, 260, 306
SfaNI GCATC 2 cut(s) 46, 218
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 1 cut(s) 386
SsiI CCGC 2 cut(s) 72, 461
SspMI CTAG 2 cut(s) 305, 452
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 3 cut(s) 105, 121, 260
TasI AATT 1 cut(s) 386
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 4 cut(s) 227, 342, 360, 390
Tru9I TTAA 4 cut(s) 227, 342, 360, 390
TscAI CASTG 3 cut(s) 139, 225, 400
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 99, 257, 263, 387
TspGWI ACGGA 2 cut(s) 193, 447
TspRI CASTG 3 cut(s) 139, 225, 400
Vha464I CTTAAG 1 cut(s) 341
XapI RAATTY 1 cut(s) 386
XceI RCATGY 1 cut(s) 442
XspI CTAG 2 cut(s) 305, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.