Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0007547

Physical Location & Seq

Genomic Coordinates Forward (+)

70044655 .. 70051098

Gene Structure 6444 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0575400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6393 bp

ATGGCGGGAGGTGGTGGCGCGGAAGCTCACGCGCGAAGCAACCAATACCAGCCGCGATCGAATTCCAGTCTGGTCCTCCTGGCGTCCTCCGACTCTCGCTCACGTGAGGGTCACGTGCCCAGCGGCGAGGCTGAGTCTCTCCGCGGCAGAATCGACCCCAAAAGCTTCGGTGATAGGGTTCGCAGGGGAAGGCCGAAAAGGCACCGCCGCGACCCGAGTCCTCCGGAACCTTTTGCTTCGAGCAAACGGCGCCGTATTTGGGAAGAGAGCGTGCTCACTGCGACGGAGGAAGACGGCGTCGTTTACAGGCCGAAGACGAAGGAGACCCGGGCTGCGTACGAGGCGATGCTTCACGTGATTCGGCGGCTGTTGGGTGACCAGCCGGCGAGTGTGGTGTACGGCGCCGCCGACGAGGCTCTGGCGATGATTAAAGGTAAGAGATTTGATAAGAGAAAAGAGATTGAGAATTTGTTGAATTCAGTCGGTGATAATGTATTTGATCAGTTTGTTCAGATTGGGAAGCTGATCACCGATTACGGTGATGATGACGACGACGACGACATAGTTGGTTGTGGTGATGGTGATTTCGATGTGGGAGTTGCGGTTGATTTTGAAGAGGATGAGGATGAGGGTGTTGGTGCGGTTGTGGAGGATGAGGAGGAGGAACAAAAGGAGTCGGTGGCGGGCGGAGCGATTGTTGATGGTGATGATGTAATGGTGGAATGTAGTGAAGGTGTGAGTGTGAATGTTCATGAGATTGATGCTTACTGGGTGCAGAGGAAGATATATGAGGCTTATGAGAAACAGATTGAGCCACAACAGTGCCAGGAGCTAGCTGAAGAGGTGGTTAGTATACTCGGTGAAGGTGGTGTTAGGGATGTGGAATCCAGGCTTTTGGAGAAGCTCCGGTTTGACAAGTTTGGGCTTATTAAGTTTTTGTTGCTGAACAGGGTGAAGATTGTGTGGTGTACTCGTTTGGCTAGAGCTGGGGGACAGGAGGAGAGGAAGAGAATCGAGGAGGAGATGTTGCGTTTGGGTCCTGATTCGGCTGCAATTGTAGAGGAGCTGTGTTTGATGAGGGCTAGTGTGAAACAGAGGCAAAAGAGTAAGGAGATGAGTATTAAAGCAGAGGCTCGGAAGTTGAAGAAGGCTGGGGTTGTTGACAGAGATGGAGAAAGTGGTGATTGGTGGAAGAACCAGGATCAGCAGCTTGATCTCGACAGCATTGCACAAGAACAAAGTAGAAGATTTTTGGTGCCAAACAAGTTTGAGCTTCCGAAAGGGTCTTACAGACATTCAAGCAAGGGATATGAAGAAATCCATGTGCCGGCATTGAAGCCTAGACCTTTCAAGTCTGATGAGAAACTTGTGCTAGTATCTGCCATGCCAGAATGGGCTCAACCGGCTTTCAAAGGAATGACTCAGTTGAATAGGGTACAAAGTCGAGTATATAGGACTGCCCTTGAAGCAGGGGACAATATTCTCTTATGTGCTCCTACTGGGTCTGGAAAAACTAATGTTGCAGTGCTCACTATACTTCAGCAGTTGGGGCTGCACATGAATCAGGAGGACGGTTCAATAGACCACAGTAAGTACAAAATTGTATATATTGCGCCCATGAAAGCTCTTGTTGCTGAAGTTGTTGGAAATCTGTCTAATCGTTTGCAGGACTATGGTGTCACGGTGAGGGAGCTAAGTGGTGACCACACACTGACTCACCAACAAATTGAAGAGACTCAAATTATCGTCTCAACTCCTGAGAAGTGGGACATCATTACCAGGAAGTCGGGAGATTATACTTGTACAAAACGGGTCAAGCTTCTCATCATTGACGAAATTCATCTTCTCCATGATAGTAGAGGCCCGGTTCTTGAAAGTATTGTTGCCCGAACTTTCAGGCAGATTGAGACCACAAAAGAGCATGTCCGCCTGGTGGGATTATCTGCTACACTTCCTAACTATGAAGATGCGGCATCATTCTTGCGTGTTGATAAGAAGGGTCTGTTTTATTTTGACAATAGTTGCAGACCTATCCCTCTTTCGCAACAGTATATTGGAATTACTGCAAGGAAGTCATGGCAGAAGTACCAGTTGATGAAGCAACTCTGCTATGAAAAGGTGATGGCTGTAGCTGGAAAACATCAAGTTCTTATTTTTGTACACTCAAGGAAAGAAACAGCTAAAACAGCTGGTGATATAAGAGATTCTGCACTAGCTAATGATTCCCTTGGTAGATTTCTGAAAGAAGACAGCGGTAGCTGTGAAATCATTCGCAGTGAATTGGTCAAAATCAAGAGCAATGATCTCAAAAGTCTTTTACCATATGGTTTTGCTATTCATCATGCTGGTATGGACAGAGCAGACCGCCAACTGGTTGAGGATCTCTTTGCTAAAGGGCATGTACAAGTTTTGGTTTCCACTGCAACGCTTGCTTGGGGTGTGAATCTGCCAGCTCACACTGTGATTATCAAAGGGACCGAAATCTATGATCCAGAAAAGGGAGCATGGACTGAACTAAGTCCGCTTGATGTTATGCAGATGTTGGGTCGTGCAGGAAGACCTCAGTATGATTCGTGTGGAGAAGGGATAATCATTACTGGCCACAAGGAACTACATCACTATCTTTCTTTAATGAACCAACAACTTCCTATTGAAAGTCAATTTCTGTCCAAGTTGGCTGATCAATTGAATGCAGAAATTGTTCTTGGAAATGTTCAGAATGCTAGAGAAGCTTCTCATTGGTTAAAATACACTTACATGTATGTTCGCATGCTAAAAAGTCCTATCCTTTATGGCTTGGAAGCTGATGTTCTGACAAAAGATAGTAAGTTGGAGGAGAGGCGAGCTGATTTGATCCATTCTGCTGCAACCATCTTGGAAAAGAACAATATGATTGTATATGATAGAAAAAGTGGATACTTCCAGGTTACAGACTTGGGTCGCATTGCCAGTCATTATTATATAACACATGGAACAGTGTCCACATATAATGAGCATTTAAAGCCAACGATGGGGGACTGTGAGCTTTGTCGATTGTTCTCTCTCAGTGAAGAATTTAAGTATGTAACTGTGCGGCAGGATGAAAAGATTGAATTGGCAAAACTTTTGGACCGCGTACCAATTGTGGTCAAGGAAAGCCTGGACGAGCCCAGTGCCAAGATTAATGTTTTGCTGCAAGCCTATATTTCACAGCTGAAGCTTGAAGGTTTTTCATTGGCATCTGATATGAGTTTTATTACTCAGAGTGCAGGGCGCCTTCTTCGAGCTCTTTTTGAGATTGCATTGAAACGAGGATGGGCTGAAGTGGCAGAAAAGGCTTTGAACTTGTGTAAAATGGTTAGCAGGAGGATGTGGACTGTCCAAACACCGTTTCGCCAATTCAATGGATTGATAACAAATGATATTTTGATGAAGTTAGAGAAGAAGGATTTAGCCTGGGAAAGGTATTATGACCTCTCTTCACAGGAGCTAGGGGAACTTATTCATTTCCCTACTTTGGGCAGAACACTTCATAAGTTGGTCCACCAGTTCCCCAAATTAAACCTCGCAGCCCATTTGCAGCCAATTACTCACACAGTATTGAAATTTGAGCTCACTATAACACCGGATTTTCAATGGGAGGACAAAGTTCATGGATATGTGGAGCCATTTTGGGTAATAGTGGAGAATAATGATCGAGAGCATATTCTTCATCATGAATATTTCCTGCTGAAAAAACAGTATATGGATGAAGATCATACTTTGATCTTTACGGTGCCAATATATGAACCTCTTCCACCTCAATATTTTATCCGTGTTGTGTCTGATAGGTGGCTTAGGTCCGAGACTGTTTTACCTGTTTTATTCAGGGACCTCATCTTACCTGCAAAGTGTCCTCCACCGACTGAGTTATTGGACTTGCAACCACTTCCTGTGACTGCATTGAGGAATCCGTTATATGAGGCTCTGTATGATCAAGATCTTATGCATTTCAATTCTGTCCAGACTCAGGTCTTCACTGCTTTGTATAATTCAGATGACAATGTTTTAGTTGCTGCACGAACAGGAAATGGGAAGACCATATGTGCAGAGTTTGCGATATTGAGGAATCATCAGAAGAGCACTGAACCTGAAGGTTGTGTCATGCGTGTTGTGTACATTGCACCTTTTGATGCAATTGCCGAGGTAACTTATGTTGACTGGAAGAAGAAATTTGGAGATGGTCTCAAACTGAACGTTGTAAAATTAACTGGGGAATTAGCCATGGACTTGAAATTGCTCGAGCAAGGTCAAGTTATCATCAGCACTCCAGAGAAATGGGACTATCTATCCCGTTGCTGGAAACGGAGAGAGAATATTCAACTAGTTAGTCTTTTTATTGTGGATGAACTCCACCTGATTAGTGGTGAGGGCGGACAGATCATAGAGACAATAGTCTCTAGAATGAGACTGATAGCCAGTCGACCCAAGACCAAGATTCGCATTGTGGCTCTTTCAACTTCTGTTGCAAATGCAAAGGACCTTGGAGAATGGATAGGAGATGCCTGCATTTTCAATTTTTCTCCAGGTGTGCGTCAAGTGCCTCTGGATATTGACATTCAGGGGGTTGATTTGGCAAACTTTTATGCAAGGATGGAAGCTATGGTGAAAGCAACATACACTGCAATTGTTCAACATGCCAAGAATGGGAAACAAGCTCTTGTGTACGTTCCTACAATGAAACATGTCCGACTAACTGCTAAGAATCTGGTGACTTACTCAAATTCTGACACTGTCGAGAAGTTGTCATTTTCATTGCAGTTTTCAGAAGATATTGGAAAAGTTAGTGATGAAAAACTGCGAAGCACTTTGAGCTGTGGGGTGGGCTACTTGCATGAGGGATTACCCAGCGAGGATCAAGAAGCTGTGACAAAGCTTTTTAAAGATCGATCAATCCAAGTCTGTGTCGTGAGCAGTTCAATGTGTTGGGGAGTGCCGTTGTCAGCCCATTTGGTGGTTGTGATGGGAACCCAGTATTATGATGGCACTGATTACCCTGTTACTGATCTGTTGCAGATGATGGGTCATGCCAATCTCCCGCAGCCAAATGATTCTGGGAAATGTGTTATCCTTTGTCACGCGCCTCGTATAGAATACTACAGGAAATTCTTGTACAAAGCATTCCCTGTTGAAAGCCAATTGCACCATTACCTACATGATATCCTAAATGCTGAAGTTGTTTCTGGTATTATTGAAACCATGCAAGACGCTGTTGATTACCTTAGTTGGACTTTCTTGTTTAGGAGGCTGACGCAAAATCCCAACTATTATGAACTCCAGGGAGTTACTTACCGACATTTATCTGATCACCTCTCAAGGCTTGTTGAAACTACAGTTTATGATTTGAAAAGAAGTGGGTGTGTTAGCATCGAGGATGACATGGATCTTTATCCTTTGAACCTTGGTGTGATAGCTACATATTATCACGCTAAGTATACTACCATCGAGCGTTTCAGTTCTTCCTTAACTTCCAAAACAACAGTGAAGGGTCTTCTGCAGATTCTAGCTCAAGCTTCGGAGTTTTCAGAACTTCCTATACGACCAGGGGAGGAAGAGGTGATCCAAAGGTTAGCAAACCACCAGAGGTTTTCCTTTGAGAATCCCAAATACACAGATTCTCATGTTAAAGCACTTGCTCTGCTTCAGGCCCATTTCTCTAGACAACCTGTGGGTGCGGAGCTGGCTTTGGACCAGCAAGAGGTGCTGACAAATGCAAGTAGGTTGATTCATGCAATGGTTGATGTGTGTTGCACCAACGGGTGGCTAAGAGTTGCTCTTCTAGTGATGGAAGTCAGCCAGATGCTGACCCAGGGATTGTGGAAGGGTGACTCAGAGTTTCTACAGCTTCCTCACTTCACAAAGGAGTTGGCAAAGAGATGCAAAGAAAATCCTGGAAAGAGTGTGCAAACCATATCTGATTTGAAGGAGATGAAGGATGACGAGAGGCATCAACTCCTTCAGTTGCCAGAGACGGAGTTGATGGCCATTGAACATTTTTGCGAACAATTTCCTGACATTGTGATAGGTTATGAGGTGCTGGAGAGTGAGAATGTACGGGCCGGGGAAGAATTCACTCTGGAAGTGAATCTCGAGTTTAGGCATAAGGTGAGAACAGCCCAGTTGGGCTGTGATGACGTGAAGAGGTACCGCAAGGACAAAGAAGACGGGTGTTGGGTTGTGGTGAGCGATAACAAGCTTGACTCAGTTGTCTTTATAAAGAGAGTTCCCCATGTTAAGAAGTTGACGAAACTCCCGGTTATAATTGCTGCTCCTGCAAAAGCTGGAACGACGGCACAGTATAAGCTTTTTTTGATGTGTGATTCCTATATGGGATGCGATCAGGAATCTGACTTGATTCCTGTTGAGATAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp12 WD40

Protein Analysis

2130

Amino Acids

241.53

Weight (kDa)

5.96

Isoelectric Point (pI)

43.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRR2_plug	PF21188	109 - 171	5e-06	Pre-mRNA-splicing helicase BRR2 plug domain
Helicase_PWI	PF18149	247 - 356	1.9e-29	N-terminal helicase PWI domain
ResIII	PF04851	477 - 652	1.7e-12	Type III restriction enzyme, res subunit
DEAD	PF00270	478 - 656	6.9e-33	DEAD/DEAH box helicase
Helicase_C	PF00271	756 - 854	2.8e-10	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	885 - 961	2e-21	Winged Helix-turn-helix domain
Sec63	PF02889	977 - 1280	4e-96	Sec63 Brl domain
ResIII	PF04851	1323 - 1468	1.1e-08	Type III restriction enzyme, res subunit
DEAD	PF00270	1326 - 1499	5.6e-25	DEAD/DEAH box helicase
WHD_SNRNP200	PF23445	1717 - 1798	2.3e-34	Winged Helix-turn-helix domain
Sec63	PF02889	1804 - 2123	1.7e-57	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007547)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0174141 RchiOBHm_Chr2g0174151 RchiOBHm_Chr5g0012111
rosa_laevigata	RLG00000022250 RLG00000022251
rosa_multiflora	Rmu_sc0003188.1_g000038 Rmu_sc0038402.1_g000002
rosa_roxburghii	Rroxscaffold_2G00078420
rosa_rugosa	Rorug02G0575000 Rorug02G0575100 Rorug02G0575100 Rorug02G0575300 Rorug02G0575400
rosa_samantha	Rh2AG654600 Rh2AG654700 Rh2AG654800 Rh2BG665800 Rh2BG666000 Rh2CG630000 Rh2CG630100 Rh2DG680400 Rh2DG680500 Rh7BG432400
rosa_wichuraiana	Rw2G053730 Rw2G053740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	6233, 6278
AasI	GACNNNNNNGTC	2 cut(s)	1676, 6224
Acc36I	ACCTGC	1 cut(s)	3871
Acc65I	GGTACC	1 cut(s)	6162
AccB1I	GGYRCC	7 cut(s)	201, 249, 401, 1255, 3252, 3754, 6162
AccB7I	CCANNNNNTGG	2 cut(s)	4972, 5778
AccI	GTMKAC	3 cut(s)	853, 4441, 5453
AccII	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
AccIII	TCCGGA	1 cut(s)	223
AclI	AACGTT	1 cut(s)	4215
AclWI	GGATC	7 cut(s)	1209, 2388, 2483, 2848, 4881, 5409, 5572
AcoI	YGGCCR	2 cut(s)	2599, 6000
AcuI	CTGAAG	9 cut(s)	858, 1523, 1656, 3215, 3321, 4131, 5211, 5645, 5960
AcvI	CACGTG	3 cut(s)	104, 115, 355
AcyI	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
AdeI	CACNNNGTG	1 cut(s)	2461
AflIII	ACRYGT	2 cut(s)	2757, 4702
AhlI	ACTAGT	1 cut(s)	4342
AjiI	CACGTC	1 cut(s)	6154
AjuI	GAANNNNNNNTTGG	4 cut(s)	2688, 2720, 3354, 3386
AleI	CACNNNNGTG	1 cut(s)	820
Alw21I	GWGCWC	6 cut(s)	276, 1495, 1530, 3268, 3594, 4103
AlwI	GGATC	7 cut(s)	1209, 2388, 2483, 2848, 4881, 5409, 5572
AlwNI	CAGNNNCTG	1 cut(s)	5821
Ama87I	CYCGRG	4 cut(s)	214, 327, 4259, 6107
Aor13HI	TCCGGA	1 cut(s)	223
AoxI	GGCC	7 cut(s)	191, 308, 1861, 2599, 5664, 6000, 6075
AseI	ATTAAT	1 cut(s)	3162
Asp700I	GAANNNNTTC	5 cut(s)	2268, 2700, 3480, 3771, 6024
Asp718I	GGTACC	1 cut(s)	6162
AspLEI	GCGC	7 cut(s)	20, 34, 252, 404, 1615, 3255, 5101
AsuC2I	CCSGG	5 cut(s)	328, 329, 1865, 6079, 6272
AsuNHI	GCTAGC	1 cut(s)	832
AvaI	CYCGRG	4 cut(s)	214, 327, 4259, 6107
AvaII	GGWCC	9 cut(s)	73, 1037, 2475, 3109, 3520, 3819, 3850, 4498, 5707
BaeGI	GKGCMC	1 cut(s)	120
BalI	TGGCCA	2 cut(s)	2601, 6002
BanI	GGYRCC	7 cut(s)	201, 249, 401, 1255, 3252, 3754, 6162
BanII	GRGCYC	4 cut(s)	1399, 3150, 3268, 3594
BbrPI	CACGTG	3 cut(s)	104, 115, 355
BbsI	GAAGAC	8 cut(s)	297, 320, 2253, 2563, 3985, 4061, 5501, 6186
Bbv12I	GWGCWC	6 cut(s)	276, 1495, 1530, 3268, 3594, 4103
BceAI	ACGGC	6 cut(s)	237, 263, 310, 415, 4939, 6324
BcgI	CGANNNNNNTGC	4 cut(s)	1208, 1242, 3134, 3168
BciVI	GTATCC	1 cut(s)	2909
BclI	TGATCA	5 cut(s)	499, 525, 2680, 3952, 5323
BcnI	CCSGG	5 cut(s)	328, 329, 1865, 6079, 6272
BcuI	ACTAGT	1 cut(s)	4342
BfmI	CTRYAG	5 cut(s)	2127, 5116, 5349, 5513, 5858
BfoI	RGCGCY	3 cut(s)	253, 405, 3256
BfuAI	ACCTGC	1 cut(s)	3871
BfuI	GTATCC	1 cut(s)	2909
BglI	GCCNNNNNGGC	2 cut(s)	199, 413
BglII	AGATCT	1 cut(s)	3958
Bme18I	GGWCC	9 cut(s)	73, 1037, 2475, 3109, 3520, 3819, 3850, 4498, 5707
BmeT110I	CYCGRG	4 cut(s)	214, 327, 4259, 6107
BmgBI	CACGTC	1 cut(s)	6154
BmrI	ACTGGG	6 cut(s)	778, 1509, 3144, 4239, 4984, 6130
BmtI	GCTAGC	1 cut(s)	836
BmuI	ACTGGG	6 cut(s)	778, 1509, 3144, 4239, 4984, 6130
BoxI	GACNNNNGTC	4 cut(s)	216, 2937, 3989, 4412
BpiI	GAAGAC	8 cut(s)	297, 320, 2253, 2563, 3985, 4061, 5501, 6186
BplI	GAGNNNNNCTC	2 cut(s)	4188, 4220
BpmI	CTGGAG	4 cut(s)	4272, 4527, 5279, 6077
Bpu10I	CCTNAGC	1 cut(s)	3815
BpuEI	CTTGAG	3 cut(s)	2149, 5317, 5511
BpuMI	CCSGG	5 cut(s)	328, 329, 1865, 6079, 6272
Bsa29I	ATCGAT	1 cut(s)	4906
BsaAI	YACGTR	3 cut(s)	104, 115, 355
BsaBI	GATNNNNATC	3 cut(s)	3732, 3957, 5406
BsaHI	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
BsaI	GGTCTC	3 cut(s)	317, 1901, 4208
BsaWI	WCCGGW	3 cut(s)	223, 906, 3604
BsaXI	ACNNNNNCTCC	6 cut(s)	588, 618, 681, 711, 1010, 1040
Bse118I	RCCGGY	3 cut(s)	382, 1327, 1402
Bse3DI	GCAATG	6 cut(s)	1224, 2305, 2943, 4137, 4772, 5757
Bse8I	GATNNNNATC	3 cut(s)	3732, 3957, 5406
BseAI	TCCGGA	1 cut(s)	223
BseCI	ATCGAT	1 cut(s)	4906
BseJI	GATNNNNATC	3 cut(s)	3732, 3957, 5406
BseMI	GCAATG	6 cut(s)	1224, 2305, 2943, 4137, 4772, 5757
BseRI	GAGGAG	7 cut(s)	671, 674, 1013, 1031, 1034, 1076, 2849
BseSI	GKGCMC	1 cut(s)	120
BseYI	CCCAGC	4 cut(s)	119, 986, 1151, 4865
BsgI	GTGCAG	7 cut(s)	794, 1538, 2193, 2571, 3267, 4020, 4086
Bsh1236I	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
Bsh1285I	CGRYCG	1 cut(s)	59
BshFI	GGCC	7 cut(s)	193, 310, 1863, 2601, 5666, 6002, 6077
BshNI	GGYRCC	7 cut(s)	201, 249, 401, 1255, 3252, 3754, 6162
BshVI	ATCGAT	1 cut(s)	4906
BsiEI	CGRYCG	1 cut(s)	59
BsiHKAI	GWGCWC	6 cut(s)	276, 1495, 1530, 3268, 3594, 4103
BsiHKCI	CYCGRG	4 cut(s)	214, 327, 4259, 6107
BsiWI	CGTACG	1 cut(s)	336
BslFI	GGGAC	7 cut(s)	1005, 1487, 1781, 2488, 3029, 3863, 4313
BsmBI	CGTCTC	2 cut(s)	1753, 5981
BsmFI	GGGAC	7 cut(s)	1005, 1487, 1781, 2488, 3029, 3863, 4313
BsmI	GAATGC	3 cut(s)	2695, 2725, 5138
BsnI	GGCC	7 cut(s)	193, 310, 1863, 2601, 5666, 6002, 6077
Bso31I	GGTCTC	3 cut(s)	317, 1901, 4208
BsoBI	CYCGRG	4 cut(s)	214, 327, 4259, 6107
Bsp1286I	GDGCHC	9 cut(s)	120, 276, 1399, 1495, 1530, 3150, 3268, 3594, 4103
Bsp13I	TCCGGA	1 cut(s)	223
Bsp1407I	TGTACA	5 cut(s)	1802, 2158, 2401, 4134, 5130
Bsp19I	CCATGG	1 cut(s)	4242
BspANI	GGCC	7 cut(s)	193, 310, 1863, 2601, 5666, 6002, 6077
BspDI	ATCGAT	1 cut(s)	4906
BspEI	TCCGGA	1 cut(s)	223
BspFNI	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
BspHI	TCATGA	2 cut(s)	751, 3694
BspMAI	CTGCAG	1 cut(s)	5517
BspMI	ACCTGC	1 cut(s)	3871
BspOI	GCTAGC	1 cut(s)	836
BspPI	GGATC	7 cut(s)	1209, 2388, 2483, 2848, 4881, 5409, 5572
BspQI	GCTCTTC	2 cut(s)	4091, 5799
BspT107I	GGYRCC	7 cut(s)	201, 249, 401, 1255, 3252, 3754, 6162
BspTNI	GGTCTC	3 cut(s)	317, 1901, 4208
BsrDI	GCAATG	6 cut(s)	1224, 2305, 2943, 4137, 4772, 5757
BsrFI	RCCGGY	3 cut(s)	382, 1327, 1402
BsrGI	TGTACA	5 cut(s)	1802, 2158, 2401, 4134, 5130
BssAI	RCCGGY	3 cut(s)	382, 1327, 1402
BssNAI	GTATAC	2 cut(s)	854, 5454
BssNI	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
BssT1I	CCWWGG	4 cut(s)	2227, 4242, 4501, 5419
Bst1107I	GTATAC	2 cut(s)	854, 5454
BstACI	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
BstAPI	GCANNNNNTGC	3 cut(s)	2429, 4073, 5719
BstAUI	TGTACA	5 cut(s)	1802, 2158, 2401, 4134, 5130
BstBAI	YACGTR	3 cut(s)	104, 115, 355
BstDSI	CCRYGG	2 cut(s)	142, 4242
BstEII	GGTNACC	2 cut(s)	374, 1700
BstENI	CCTNNNNNAGG	1 cut(s)	3439
BstFNI	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
BstH2I	RGCGCY	3 cut(s)	253, 405, 3256
BstHHI	GCGC	7 cut(s)	20, 34, 252, 404, 1615, 3255, 5101
BstMCI	CGRYCG	1 cut(s)	59
BstNSI	RCATGY	6 cut(s)	1925, 2402, 2761, 2773, 4658, 4706
BstPAI	GACNNNNGTC	4 cut(s)	216, 2937, 3989, 4412
BstPI	GGTNACC	2 cut(s)	374, 1700
BstSFI	CTRYAG	5 cut(s)	2127, 5116, 5349, 5513, 5858
BstSLI	GKGCMC	1 cut(s)	120
BstUI	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
BstV2I	GAAGAC	8 cut(s)	297, 320, 2253, 2563, 3985, 4061, 5501, 6186
BstX2I	RGATCY	3 cut(s)	2380, 3958, 5401
BstXI	CCANNNNNNTGG	3 cut(s)	895, 3383, 4296
BstYI	RGATCY	3 cut(s)	2380, 3958, 5401
BstZ17I	GTATAC	2 cut(s)	854, 5454
Bsu15I	ATCGAT	1 cut(s)	4906
BsuI	GTATCC	1 cut(s)	2909
BsuRI	GGCC	7 cut(s)	193, 310, 1863, 2601, 5666, 6002, 6077
BsuTUI	ATCGAT	1 cut(s)	4906
BtgI	CCRYGG	2 cut(s)	142, 4242
BtgZI	GCGATG	2 cut(s)	359, 437
BtrI	CACGTC	1 cut(s)	6154
BtsI	GCAGTG	6 cut(s)	276, 1530, 2281, 2418, 3996, 4638
BveI	ACCTGC	1 cut(s)	3871
CaiI	CAGNNNCTG	1 cut(s)	5821
CciI	TCATGA	2 cut(s)	751, 3694
CfoI	GCGC	7 cut(s)	20, 34, 252, 404, 1615, 3255, 5101
Cfr10I	RCCGGY	3 cut(s)	382, 1327, 1402
Cfr42I	CCGCGG	1 cut(s)	145
Cfr9I	CCCGGG	1 cut(s)	327
ClaI	ATCGAT	1 cut(s)	4906
CseI	GACGC	5 cut(s)	72, 286, 4541, 5234, 5278
DinI	GGCGCC	3 cut(s)	251, 403, 3254
DraI	TTTAAA	2 cut(s)	3000, 4900
DraIII	CACNNNGTG	1 cut(s)	2461
DrdI	GACNNNNNNGTC	2 cut(s)	1676, 6224
DseDI	GACNNNNNNGTC	2 cut(s)	1676, 6224
EaeI	YGGCCR	2 cut(s)	2599, 6000
EciI	GGCGGA	3 cut(s)	702, 1916, 4407
Ecl136II	GAGCTC	2 cut(s)	3266, 3592
Eco130I	CCWWGG	4 cut(s)	2227, 4242, 4501, 5419
Eco24I	GRGCYC	4 cut(s)	1399, 3150, 3268, 3594
Eco31I	GGTCTC	3 cut(s)	317, 1901, 4208
Eco32I	GATATC	1 cut(s)	5179
Eco47I	GGWCC	9 cut(s)	73, 1037, 2475, 3109, 3520, 3819, 3850, 4498, 5707
Eco53kI	GAGCTC	2 cut(s)	3266, 3592
Eco57I	CTGAAG	9 cut(s)	858, 1523, 1656, 3215, 3321, 4131, 5211, 5645, 5960
Eco72I	CACGTG	3 cut(s)	104, 115, 355
Eco88I	CYCGRG	4 cut(s)	214, 327, 4259, 6107
Eco91I	GGTNACC	2 cut(s)	374, 1700
EcoICRI	GAGCTC	2 cut(s)	3266, 3592
EcoNI	CCTNNNNNAGG	1 cut(s)	3439
EcoO109I	RGGNCCY	3 cut(s)	1037, 3850, 4498
EcoO65I	GGTNACC	2 cut(s)	374, 1700
EcoRI	GAATTC	3 cut(s)	61, 475, 6086
EcoRV	GATATC	1 cut(s)	5179
EcoT14I	CCWWGG	4 cut(s)	2227, 4242, 4501, 5419
EcoT22I	ATGCAT	1 cut(s)	3969
EcoT38I	GRGCYC	4 cut(s)	1399, 3150, 3268, 3594
EgeI	GGCGCC	3 cut(s)	251, 403, 3254
EheI	GGCGCC	3 cut(s)	251, 403, 3254
ErhI	CCWWGG	4 cut(s)	2227, 4242, 4501, 5419
Esp3I	CGTCTC	2 cut(s)	1753, 5981
FaqI	GGGAC	7 cut(s)	1005, 1487, 1781, 2488, 3029, 3863, 4313
FauI	CCCGC	2 cut(s)	676, 5064
FauNDI	CATATG	2 cut(s)	2323, 4061
FbaI	TGATCA	5 cut(s)	499, 525, 2680, 3952, 5323
FblI	GTMKAC	3 cut(s)	853, 4441, 5453
FriOI	GRGCYC	4 cut(s)	1399, 3150, 3268, 3594
GlaI	GCGC	7 cut(s)	19, 33, 251, 403, 1614, 3254, 5100
GsaI	CCCAGC	4 cut(s)	123, 990, 1155, 4869
GsuI	CTGGAG	4 cut(s)	4272, 4527, 5279, 6077
HaeII	RGCGCY	3 cut(s)	253, 405, 3256
HaeIII	GGCC	7 cut(s)	193, 310, 1863, 2601, 5666, 6002, 6077
HgaI	GACGC	5 cut(s)	72, 286, 4541, 5234, 5278
HhaI	GCGC	7 cut(s)	20, 34, 252, 404, 1615, 3255, 5101
Hin1I	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
Hin6I	GCGC	7 cut(s)	18, 32, 250, 402, 1613, 3253, 5099
HinP1I	GCGC	7 cut(s)	18, 32, 250, 402, 1613, 3253, 5099
HincII	GTYRAC	4 cut(s)	1162, 4177, 4442, 6261
HindII	GTYRAC	4 cut(s)	1162, 4177, 4442, 6261
HindIII	AAGCTT	8 cut(s)	163, 1817, 2730, 3197, 4892, 5529, 6212, 6320
Hpy99I	CGWCG	7 cut(s)	286, 302, 413, 554, 557, 560, 6310
HpyCH4IV	ACGT	6 cut(s)	103, 114, 354, 4215, 4686, 6153
HpySE526I	ACGT	6 cut(s)	103, 114, 354, 4215, 4686, 6153
Hsp92I	GRCGYC	5 cut(s)	83, 250, 297, 402, 3253
HspAI	GCGC	7 cut(s)	18, 32, 250, 402, 1613, 3253, 5099
KasI	GGCGCC	3 cut(s)	249, 401, 3252
Kpn2I	TCCGGA	1 cut(s)	223
KpnI	GGTACC	1 cut(s)	6166
KroI	GCCGGC	2 cut(s)	382, 1327
KroNI	GCCGGC	2 cut(s)	384, 1329
Ksp22I	TGATCA	5 cut(s)	499, 525, 2680, 3952, 5323
KspI	CCGCGG	1 cut(s)	145
LguI	GCTCTTC	2 cut(s)	4091, 5799
MaeII	ACGT	6 cut(s)	103, 114, 354, 4215, 4686, 6153
MfeI	CAATTG	6 cut(s)	1053, 2684, 3120, 4155, 4644, 5156
MflI	RGATCY	3 cut(s)	2380, 3958, 5401
MhlI	GDGCHC	9 cut(s)	120, 276, 1399, 1495, 1530, 3150, 3268, 3594, 4103
MlsI	TGGCCA	2 cut(s)	2601, 6002
MluNI	TGGCCA	2 cut(s)	2601, 6002
Mly113I	GGCGCC	3 cut(s)	250, 402, 3253
MmeI	TCCRAC	5 cut(s)	114, 1624, 2811, 4732, 5225
Mox20I	TGGCCA	2 cut(s)	2601, 6002
Mph1103I	ATGCAT	1 cut(s)	3969
MroI	TCCGGA	1 cut(s)	223
MroNI	GCCGGC	2 cut(s)	382, 1327
MroXI	GAANNNNTTC	5 cut(s)	2268, 2700, 3480, 3771, 6024
MscI	TGGCCA	2 cut(s)	2601, 6002
MslI	CAYNNNNRTG	3 cut(s)	820, 2756, 3636
Msp20I	TGGCCA	2 cut(s)	2601, 6002
MspA1I	CMGCKG	5 cut(s)	123, 144, 2189, 2253, 3193
MunI	CAATTG	6 cut(s)	1053, 2684, 3120, 4155, 4644, 5156
Mva1269I	GAATGC	3 cut(s)	2695, 2725, 5138
MvnI	CGCG	8 cut(s)	20, 32, 34, 55, 144, 210, 3114, 5099
NaeI	GCCGGC	2 cut(s)	384, 1329
NarI	GGCGCC	3 cut(s)	250, 402, 3253
NciI	CCSGG	5 cut(s)	328, 329, 1865, 6079, 6272
NcoI	CCATGG	1 cut(s)	4242
NdeI	CATATG	2 cut(s)	2323, 4061
NgoMIV	GCCGGC	2 cut(s)	382, 1327
NheI	GCTAGC	1 cut(s)	832
NmeAIII	GCCGAG	1 cut(s)	4186
NmuCI	GTSAC	9 cut(s)	110, 374, 1678, 1700, 3913, 4729, 4885, 5093, 5843
NsiI	ATGCAT	1 cut(s)	3969
NspI	RCATGY	6 cut(s)	1925, 2402, 2761, 2773, 4658, 4706
OliI	CACNNNNGTG	1 cut(s)	820
PaeI	GCATGC	1 cut(s)	2773
PaeR7I	CTCGAG	2 cut(s)	4259, 6107
PagI	TCATGA	2 cut(s)	751, 3694
PasI	CCCWGGG	1 cut(s)	5827
PciI	ACATGT	2 cut(s)	2757, 4702
PciSI	GCTCTTC	2 cut(s)	4091, 5799
PcsI	WCGNNNNNNNCGW	2 cut(s)	405, 555
PctI	GAATGC	3 cut(s)	2695, 2725, 5138
PdiI	GCCGGC	2 cut(s)	384, 1329
PdmI	GAANNNNTTC	5 cut(s)	2268, 2700, 3480, 3771, 6024
Pfl23II	CGTACG	1 cut(s)	336
PflFI	GACNNNGTC	2 cut(s)	296, 4751
PflMI	CCANNNNNTGG	2 cut(s)	4972, 5778
PfoI	TCCNGGA	1 cut(s)	5908
Ple19I	CGATCG	1 cut(s)	59
PluTI	GGCGCC	3 cut(s)	253, 405, 3256
PmaCI	CACGTG	3 cut(s)	104, 115, 355
PmlI	CACGTG	3 cut(s)	104, 115, 355
Ppu21I	YACGTR	3 cut(s)	104, 115, 355
PpuMI	RGGWCCY	3 cut(s)	1037, 3850, 4498
PscI	ACATGT	2 cut(s)	2757, 4702
PshAI	GACNNNNGTC	4 cut(s)	216, 2937, 3989, 4412
PshBI	ATTAAT	1 cut(s)	3162
PsiI	TTATAA	2 cut(s)	6233, 6278
Psp124BI	GAGCTC	2 cut(s)	3268, 3594
Psp1406I	AACGTT	1 cut(s)	4215
Psp5II	RGGWCCY	3 cut(s)	1037, 3850, 4498
PspCI	CACGTG	3 cut(s)	104, 115, 355
PspEI	GGTNACC	2 cut(s)	374, 1700
PspFI	CCCAGC	4 cut(s)	119, 986, 1151, 4865
PspLI	CGTACG	1 cut(s)	336
PspPPI	RGGWCCY	3 cut(s)	1037, 3850, 4498
PspXI	VCTCGAGB	1 cut(s)	4259
PsrI	GAACNNNNNNTAC	2 cut(s)	5538, 5570
PstI	CTGCAG	1 cut(s)	5517
PstNI	CAGNNNCTG	1 cut(s)	5821
PsuI	RGATCY	3 cut(s)	2380, 3958, 5401
PsyI	GACNNNGTC	2 cut(s)	296, 4751
PvuI	CGATCG	1 cut(s)	59
PvuII	CAGCTG	2 cut(s)	2189, 3193
RseI	CAYNNNNRTG	3 cut(s)	820, 2756, 3636
SacI	GAGCTC	2 cut(s)	3268, 3594
SacII	CCGCGG	1 cut(s)	145
SalI	GTCGAC	1 cut(s)	4440
SapI	GCTCTTC	2 cut(s)	4091, 5799
SduI	GDGCHC	9 cut(s)	120, 276, 1399, 1495, 1530, 3150, 3268, 3594, 4103
SfcI	CTRYAG	5 cut(s)	2127, 5116, 5349, 5513, 5858
SfoI	GGCGCC	3 cut(s)	251, 403, 3254
Sfr274I	CTCGAG	2 cut(s)	4259, 6107
Sfr303I	CCGCGG	1 cut(s)	145
SgrBI	CCGCGG	1 cut(s)	145
SinI	GGWCC	9 cut(s)	73, 1037, 2475, 3109, 3520, 3819, 3850, 4498, 5707
SlaI	CTCGAG	2 cut(s)	4259, 6107
SmaI	CCCGGG	1 cut(s)	329
SmiMI	CAYNNNNRTG	3 cut(s)	820, 2756, 3636
SmlI	CTYRAG	5 cut(s)	2164, 4259, 5332, 5526, 6107
SmoI	CTYRAG	5 cut(s)	2164, 4259, 5332, 5526, 6107
SpeI	ACTAGT	1 cut(s)	4342
SphI	GCATGC	1 cut(s)	2773
SspDI	GGCGCC	3 cut(s)	249, 401, 3252
SspI	AATATT	4 cut(s)	1480, 3701, 3785, 4336
SstI	GAGCTC	2 cut(s)	3268, 3594
StyI	CCWWGG	4 cut(s)	2227, 4242, 4501, 5419
TaiI	ACGT	6 cut(s)	106, 117, 357, 4218, 4689, 6156
TaqII	GACCGA	1 cut(s)	2492
TatI	WGTACW	7 cut(s)	968, 1593, 1802, 2158, 2401, 4134, 5130
TauI	GCSGC	8 cut(s)	55, 126, 147, 210, 367, 407, 1973, 3076
TseFI	GTSAC	9 cut(s)	110, 374, 1678, 1700, 3913, 4729, 4885, 5093, 5843
Tsp45I	GTSAC	9 cut(s)	110, 374, 1678, 1700, 3913, 4729, 4885, 5093, 5843
TspGWI	ACGGA	5 cut(s)	299, 3782, 3921, 4339, 6005
TspMI	CCCGGG	1 cut(s)	327
Tth111I	GACNNNGTC	2 cut(s)	296, 4751
Van91I	CCANNNNNTGG	2 cut(s)	4972, 5778
VpaK11BI	GGWCC	9 cut(s)	73, 1037, 2475, 3109, 3520, 3819, 3850, 4498, 5707
VspI	ATTAAT	1 cut(s)	3162
XagI	CCTNNNNNAGG	1 cut(s)	3439
XbaI	TCTAGA	2 cut(s)	4418, 5675
XceI	RCATGY	6 cut(s)	1925, 2402, 2761, 2773, 4658, 4706
XcmI	CCANNNNNNNNNTGG	2 cut(s)	4379, 4997
XhoI	CTCGAG	2 cut(s)	4259, 6107
XmaI	CCCGGG	1 cut(s)	327
XmiI	GTMKAC	3 cut(s)	853, 4441, 5453
XmnI	GAANNNNTTC	5 cut(s)	2268, 2700, 3480, 3771, 6024
Zsp2I	ATGCAT	1 cut(s)	3969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.